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The map label for this gene is nei [H]

Identifier: 158333840

GI number: 158333840

Start: 636856

End: 637677

Strand: Direct

Name: nei [H]

Synonym: AM1_0649

Alternate gene names: 158333840

Gene position: 636856-637677 (Clockwise)

Preceding gene: 158333839

Following gene: 158333841

Centisome position: 9.79

GC content: 48.91

Gene sequence:

>822_bases
ATGCCTGAAGGACCTGAAATTAGACGTGCTGCGGATCGTATTGCGAGTGAGATCGCAAACCAACCCCTACAGGAAGTTTG
GTTCGCCTTTTCCCACCTAAAAATATACGAAGCTCAATTAGGTGCCAGTCAAATTCAACAGGTAGAAACTCGGGGTAAAG
GTCTGCTGATGCATTTTGACTGTGGTCTCAGCATCTACAGCCATAACCAGCTTTACGGCAAGTGGATGATCCGCAAAGCC
TACAACTATCCTCAAACCAAGCGACAATTGCGGCTAGCTTTGCATTGCGATCGCAAATCCGCTCTCCTTTATAGTGCATC
AGATATTGATGTCTTGGATGCGGCGGGGATTCAGGCCCATCCCTTTCTAAGTCGGATTGGACCAGATGTTCTCAGTTCTG
AGACAACCGTCGAGCAGGTACGAGAGAGACTGAGTTGCGATCGCTTTCGCCGTCGTCGCCTCACGACACTGTTACTTGAC
CAGCATTTCCTGGCTGGGTTGGGGAATTACCTCAGAAGTGAAATCTTGTTTGTTGCCAGGGTTCATCCTTCTCTCAGACC
TGCAGATTGTACCGATGAACAGATAGAGAAATTGGCAGAAGCAGCCCTAGCTTTGCCTAGACAGTCCTATGAAACCGGAG
GTATTACCAACGATATTGCCTGGGCCGCTAAATTGAAATTCCAGGGATTTGCACGGCGTGTGTATCGACATTGGGTCTTT
GCCAGAGCAGGGCAGCTCTGTTGGGTTTGTGCTACCCCGATTGTCAAAGATGCTCAAGGTGGACGTCGGTATTATTACTG
TCCCCAATGCCAAGAAGAATGA

Upstream 100 bases:

>100_bases
AGAACTGCATACCACGGCATTCTAAAGCTGCTTTGAAGCTATTTCAGGGGGATATTACTCTATTCTTTGGAGATAGTATT
TCCCCACCGTTATTAATTCC

Downstream 100 bases:

>100_bases
GTTGTTCCACTTTATAAGCCAGTGACAGTTGGTATGGCAATCGCCATATCGAGAAGGACGTAAACTACACGCCCTTTTCT
CGTAGCTAGGCCCCTTGGTG

Product: endonuclease VIII

Products: NA

Alternate protein names: DNA glycosylase/AP lyase Nei; DNA-(apurinic or apyrimidinic site) lyase Nei; Endonuclease VIII [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPEGPEIRRAADRIASEIANQPLQEVWFAFSHLKIYEAQLGASQIQQVETRGKGLLMHFDCGLSIYSHNQLYGKWMIRKA
YNYPQTKRQLRLALHCDRKSALLYSASDIDVLDAAGIQAHPFLSRIGPDVLSSETTVEQVRERLSCDRFRRRRLTTLLLD
QHFLAGLGNYLRSEILFVARVHPSLRPADCTDEQIEKLAEAALALPRQSYETGGITNDIAWAAKLKFQGFARRVYRHWVF
ARAGQLCWVCATPIVKDAQGGRRYYYCPQCQEE

Sequences:

>Translated_273_residues
MPEGPEIRRAADRIASEIANQPLQEVWFAFSHLKIYEAQLGASQIQQVETRGKGLLMHFDCGLSIYSHNQLYGKWMIRKA
YNYPQTKRQLRLALHCDRKSALLYSASDIDVLDAAGIQAHPFLSRIGPDVLSSETTVEQVRERLSCDRFRRRRLTTLLLD
QHFLAGLGNYLRSEILFVARVHPSLRPADCTDEQIEKLAEAALALPRQSYETGGITNDIAWAAKLKFQGFARRVYRHWVF
ARAGQLCWVCATPIVKDAQGGRRYYYCPQCQEE
>Mature_272_residues
PEGPEIRRAADRIASEIANQPLQEVWFAFSHLKIYEAQLGASQIQQVETRGKGLLMHFDCGLSIYSHNQLYGKWMIRKAY
NYPQTKRQLRLALHCDRKSALLYSASDIDVLDAAGIQAHPFLSRIGPDVLSSETTVEQVRERLSCDRFRRRRLTTLLLDQ
HFLAGLGNYLRSEILFVARVHPSLRPADCTDEQIEKLAEAALALPRQSYETGGITNDIAWAAKLKFQGFARRVYRHWVFA
RAGQLCWVCATPIVKDAQGGRRYYYCPQCQEE

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothy

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786932, Length=271, Percent_Identity=47.2324723247232, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1790066, Length=156, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 31246; Mature: 31115

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGPEIRRAADRIASEIANQPLQEVWFAFSHLKIYEAQLGASQIQQVETRGKGLLMHFD
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEEE
CGLSIYSHNQLYGKWMIRKAYNYPQTKRQLRLALHCDRKSALLYSASDIDVLDAAGIQAH
CCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCCCCC
PFLSRIGPDVLSSETTVEQVRERLSCDRFRRRRLTTLLLDQHFLAGLGNYLRSEILFVAR
HHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VHPSLRPADCTDEQIEKLAEAALALPRQSYETGGITNDIAWAAKLKFQGFARRVYRHWVF
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ARAGQLCWVCATPIVKDAQGGRRYYYCPQCQEE
HHCCCEEEEECCCHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
PEGPEIRRAADRIASEIANQPLQEVWFAFSHLKIYEAQLGASQIQQVETRGKGLLMHFD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEEE
CGLSIYSHNQLYGKWMIRKAYNYPQTKRQLRLALHCDRKSALLYSASDIDVLDAAGIQAH
CCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCCCCC
PFLSRIGPDVLSSETTVEQVRERLSCDRFRRRRLTTLLLDQHFLAGLGNYLRSEILFVAR
HHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VHPSLRPADCTDEQIEKLAEAALALPRQSYETGGITNDIAWAAKLKFQGFARRVYRHWVF
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ARAGQLCWVCATPIVKDAQGGRRYYYCPQCQEE
HHCCCEEEEECCCHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA