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The map label for this gene is malZ [C]

Identifier: 158333813

GI number: 158333813

Start: 609676

End: 611136

Strand: Reverse

Name: malZ [C]

Synonym: AM1_0622

Alternate gene names: 158333813

Gene position: 611136-609676 (Counterclockwise)

Preceding gene: 158333815

Following gene: 158333812

Centisome position: 9.4

GC content: 48.46

Gene sequence:

>1461_bases
ATGCAGATTAATACGCCTGATTGGGTCAAAAATGCTGTCTTTTACCAGATTTTTCCTGATCGCTTCGCTTGCCACCCCCA
TCCATCCATCCCATCCCAAGTTAATTGGGAAGCTTGGCACGCCCCACCCACCTTCCAAGGATATAAAGGGGGAAATTTGT
GGGGGATTATTGATCGCCTCGACTATTTGGCTGATTTAGGTATCACAGCCCTCTACCTCACGCCTATTTTCCAATCAGCA
TGCAACCACCGGTATCACACCCATGATTATTACCAAGTCGACCCCATTTTAGGGGGGAATGAAGCCCTAACTGATTTGCT
TACAGCAGCCCATAAACGCCACATCAAGGTAGTTCTGGACGGTGTATTTAATCATGCCAGCCGGGGATTTTTCTTTTTTA
ATGACATCTTAGAAAATGGTCCCCACTCACCTTGGGTGGATTGGTTTCGAATTGAGGGCTGGCCTGTTGCAGCGTATCAA
GGCAGTAAGCCGGCTAACTACGTGGGCTGGAGTGGTAATCGCGCCTTACCACAGTTCAACCATGACCACCCTGATGTGCG
TGAGTACATTATGCAAATTGCGGAGTACTGGATCAAACAAGGTGTTGATGGGTGGCGGCTAGATGTGCCCAATGAAATTG
AAGTGCCCGGATTCTGGCAAGAATTTCGCGATCGCGTCAAAGGTATCAATTCAGAAGCCTATATTGTGGGTGAGATCTGG
GACAACGCCCAGCCGTGGCTACAAGGCGACCAATTTGATGGGGTGATGAATTACCAATTCCGACAAGCCACTATCGCTTT
TACAGCAGGTGATCGAGTGGATGTCGCCTATACAACACAGCCTGCCTATGCCCCTTATCCTGCCCTAGACGCATCAGACT
ATCAACAACGGATTCAGGACTTACTGACCTGGTATCCCTGGGATATTCAACTCACTCAGCTTAACTTGCTGTCCAGCCAT
GATACGGCTCGACTGCTGTCCATGGTGCACCTAGACTACAGCACCATAGAACTAGCGACCCTCTTGTTGCTCACCTTTCC
CGGTGCTCCTTGTATCTACTACGGGGATGAAGTGGGGCTTCCCGGTGGAATCGATCCCGATTGCCGTCGTAGTTTTCCTG
AGGAGGCAGATTGGCATCTACCCTTACAAACCATACACAAGCAGCTGATCCAAATTCGGCACCAGCATGCTGCCCTTAGA
ACTGGTCAATACCAAGTTTTACTGGCTACAGATGAAGCCTATGTTTTTAGCCGGTCCTTGGCAGAAGAAGTGATGATTGT
AGCCATCAACACGAGTACGAAGTCTAGCTCTCTCACCATCGATCTCAGCCCGTTATCCCCATCTATTGCAAAGTTGGACT
CTATCTACGGAACCCCTAACGTCTTAGCAATAAATACGAATACTGCAACAGTAGAGTTATCCGCACGACAAGGGAGTATT
ATGATTGCCAGTTTTGAATAA

Upstream 100 bases:

>100_bases
GTTCTTGGTTTAACCTCATTGCTACAGCTAATGAAGGGATGGCTGATTCTTCAGACAATTGTTATACCATTGCCATTACT
CTGTCGCTTAGTTGGTGATA

Downstream 100 bases:

>100_bases
ATAAGGCCTCAATTCGCTTAAACCATCGTTTTGGTATCACAGACGTCGGATTACCCTAATCCGTTGGGCATCCTAAGGAT
ATGGGGGGACCCCAGATTCG

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MQINTPDWVKNAVFYQIFPDRFACHPHPSIPSQVNWEAWHAPPTFQGYKGGNLWGIIDRLDYLADLGITALYLTPIFQSA
CNHRYHTHDYYQVDPILGGNEALTDLLTAAHKRHIKVVLDGVFNHASRGFFFFNDILENGPHSPWVDWFRIEGWPVAAYQ
GSKPANYVGWSGNRALPQFNHDHPDVREYIMQIAEYWIKQGVDGWRLDVPNEIEVPGFWQEFRDRVKGINSEAYIVGEIW
DNAQPWLQGDQFDGVMNYQFRQATIAFTAGDRVDVAYTTQPAYAPYPALDASDYQQRIQDLLTWYPWDIQLTQLNLLSSH
DTARLLSMVHLDYSTIELATLLLLTFPGAPCIYYGDEVGLPGGIDPDCRRSFPEEADWHLPLQTIHKQLIQIRHQHAALR
TGQYQVLLATDEAYVFSRSLAEEVMIVAINTSTKSSSLTIDLSPLSPSIAKLDSIYGTPNVLAINTNTATVELSARQGSI
MIASFE

Sequences:

>Translated_486_residues
MQINTPDWVKNAVFYQIFPDRFACHPHPSIPSQVNWEAWHAPPTFQGYKGGNLWGIIDRLDYLADLGITALYLTPIFQSA
CNHRYHTHDYYQVDPILGGNEALTDLLTAAHKRHIKVVLDGVFNHASRGFFFFNDILENGPHSPWVDWFRIEGWPVAAYQ
GSKPANYVGWSGNRALPQFNHDHPDVREYIMQIAEYWIKQGVDGWRLDVPNEIEVPGFWQEFRDRVKGINSEAYIVGEIW
DNAQPWLQGDQFDGVMNYQFRQATIAFTAGDRVDVAYTTQPAYAPYPALDASDYQQRIQDLLTWYPWDIQLTQLNLLSSH
DTARLLSMVHLDYSTIELATLLLLTFPGAPCIYYGDEVGLPGGIDPDCRRSFPEEADWHLPLQTIHKQLIQIRHQHAALR
TGQYQVLLATDEAYVFSRSLAEEVMIVAINTSTKSSSLTIDLSPLSPSIAKLDSIYGTPNVLAINTNTATVELSARQGSI
MIASFE
>Mature_486_residues
MQINTPDWVKNAVFYQIFPDRFACHPHPSIPSQVNWEAWHAPPTFQGYKGGNLWGIIDRLDYLADLGITALYLTPIFQSA
CNHRYHTHDYYQVDPILGGNEALTDLLTAAHKRHIKVVLDGVFNHASRGFFFFNDILENGPHSPWVDWFRIEGWPVAAYQ
GSKPANYVGWSGNRALPQFNHDHPDVREYIMQIAEYWIKQGVDGWRLDVPNEIEVPGFWQEFRDRVKGINSEAYIVGEIW
DNAQPWLQGDQFDGVMNYQFRQATIAFTAGDRVDVAYTTQPAYAPYPALDASDYQQRIQDLLTWYPWDIQLTQLNLLSSH
DTARLLSMVHLDYSTIELATLLLLTFPGAPCIYYGDEVGLPGGIDPDCRRSFPEEADWHLPLQTIHKQLIQIRHQHAALR
TGQYQVLLATDEAYVFSRSLAEEVMIVAINTSTKSSSLTIDLSPLSPSIAKLDSIYGTPNVLAINTNTATVELSARQGSI
MIASFE

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=411, Percent_Identity=23.8442822384428, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1786604, Length=505, Percent_Identity=33.8613861386139, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1790687, Length=398, Percent_Identity=23.3668341708543, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322245, Length=228, Percent_Identity=28.9473684210526, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321726, Length=231, Percent_Identity=27.7056277056277, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322241, Length=231, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322021, Length=231, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324416, Length=231, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=225, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=225, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586587, Length=424, Percent_Identity=25.4716981132075, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24586591, Length=413, Percent_Identity=24.2130750605327, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24586599, Length=555, Percent_Identity=23.963963963964, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24586593, Length=406, Percent_Identity=26.3546798029557, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24583747, Length=549, Percent_Identity=23.4972677595628, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24586589, Length=408, Percent_Identity=25.4901960784314, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221330053, Length=233, Percent_Identity=28.3261802575107, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583749, Length=226, Percent_Identity=28.7610619469027, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583745, Length=590, Percent_Identity=21.864406779661, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586597, Length=413, Percent_Identity=23.9709443099274, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45549022, Length=553, Percent_Identity=21.6998191681736, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 55176; Mature: 55176

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQINTPDWVKNAVFYQIFPDRFACHPHPSIPSQVNWEAWHAPPTFQGYKGGNLWGIIDRL
CCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEHHHHH
DYLADLGITALYLTPIFQSACNHRYHTHDYYQVDPILGGNEALTDLLTAAHKRHIKVVLD
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEEE
GVFNHASRGFFFFNDILENGPHSPWVDWFRIEGWPVAAYQGSKPANYVGWSGNRALPQFN
HHHHHCCCCEEEEHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCEECCCCCCCCCCCC
HDHPDVREYIMQIAEYWIKQGVDGWRLDVPNEIEVPGFWQEFRDRVKGINSEAYIVGEIW
CCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEC
DNAQPWLQGDQFDGVMNYQFRQATIAFTAGDRVDVAYTTQPAYAPYPALDASDYQQRIQD
CCCCCCCCCCCCCCEECEEEEEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
LLTWYPWDIQLTQLNLLSSHDTARLLSMVHLDYSTIELATLLLLTFPGAPCIYYGDEVGL
HHHCCCCEEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCC
PGGIDPDCRRSFPEEADWHLPLQTIHKQLIQIRHQHAALRTGQYQVLLATDEAYVFSRSL
CCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
AEEVMIVAINTSTKSSSLTIDLSPLSPSIAKLDSIYGTPNVLAINTNTATVELSARQGSI
CCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEEEEECCCCEE
MIASFE
EEEECC
>Mature Secondary Structure
MQINTPDWVKNAVFYQIFPDRFACHPHPSIPSQVNWEAWHAPPTFQGYKGGNLWGIIDRL
CCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEHHHHH
DYLADLGITALYLTPIFQSACNHRYHTHDYYQVDPILGGNEALTDLLTAAHKRHIKVVLD
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEEE
GVFNHASRGFFFFNDILENGPHSPWVDWFRIEGWPVAAYQGSKPANYVGWSGNRALPQFN
HHHHHCCCCEEEEHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCEECCCCCCCCCCCC
HDHPDVREYIMQIAEYWIKQGVDGWRLDVPNEIEVPGFWQEFRDRVKGINSEAYIVGEIW
CCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEC
DNAQPWLQGDQFDGVMNYQFRQATIAFTAGDRVDVAYTTQPAYAPYPALDASDYQQRIQD
CCCCCCCCCCCCCCEECEEEEEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
LLTWYPWDIQLTQLNLLSSHDTARLLSMVHLDYSTIELATLLLLTFPGAPCIYYGDEVGL
HHHCCCCEEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCC
PGGIDPDCRRSFPEEADWHLPLQTIHKQLIQIRHQHAALRTGQYQVLLATDEAYVFSRSL
CCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
AEEVMIVAINTSTKSSSLTIDLSPLSPSIAKLDSIYGTPNVLAINTNTATVELSARQGSI
CCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEEEEECCCCEE
MIASFE
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644767; 8425614 [H]