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The map label for this gene is insG [H]

Identifier: 158333730

GI number: 158333730

Start: 518372

End: 519820

Strand: Reverse

Name: insG [H]

Synonym: AM1_0538

Alternate gene names: 158333730

Gene position: 519820-518372 (Counterclockwise)

Preceding gene: 158333731

Following gene: 158333728

Centisome position: 7.99

GC content: 50.24

Gene sequence:

>1449_bases
TTGATAGATATAGGATCAAGACTTGCACGCCATAAACTTCTGCCTTTCACTATGTCTGAGTCAGCCGAAATTCTCAAGCA
GCAATTTTCCCAAAGCCTGGGTTTACCGTGGACAGATATCCTTCCAGCCTCAAGGTTAGAGGAACTCCTGAAAGAAGAAG
CATTCTCCTATCGCAACCGGATATATAGCCCCATCGTTACGCTGTGGGCCATGCTTTACCAAGTGCTATCAGCCGATAAA
AGCCTTCGTAACACAGTCAAATGCATCACGACTTGGCTCACAGCGGCTGGCATCCAACCGCCCTCATCTGATACCGGAGC
CTACAGCAAAGCCAGAAGTCGCTTTCCAGAGTCACTGTTGCAACGTTTAATTCCCGAAAGCGCTGAGTGCTTAGCGCAAC
CCCTCTCCCCAGAGCACCTCTGGTGTGGTCGGCCCGTCAAGGTCTACGACGGAACCACCGTGTTGATGGCTGATAGTGCG
GCCAACCAAGCATCATATCCACAACATGGCAATCAAACAGCAGGCTGTGGTTTTCCCATCGCTCGCTTGGTAGTGTTCTT
CTGTTTGGTTACCGGTGCAGTGGCGTCAGCTTGTATTGCCTCCTGGGACACCAGTGAAATTGTCATGAGTCGTTTGCTCT
ATCAAGACCTTGAGGTCGGTGATGTGGTCATGGCGGACCAAGCTTATGGCAGCTATGTTGATCTAGCCATCATTCAACAA
CACAGGGCTGATGGAGTGTTGCGTAAACATCATGCTCGCAAGACTGATTTCCGCAAAGGCAACAAGCATGGCATTGGTGA
TCATCAGGTGACATGGCATAAGCCAGCCCAACGGCCTGAGCACATGAGTGAGCAAGATTTTGCCCTGATTCCTCAAACAT
TGGTCGTTAGAGAGGTGTGTTTGCGCTTATCCCTTAAGGGCTTTCGCGACCAGCACATTATTGTGGTGACGACGCTGCTG
GATGCTCAACGCTACAGCGCTGGGCAACTGACTCGCTTGTATGGCTGGCGTTGGCCAGTGGCGGAAGTCAATCTGCGCCA
TCTCAAAACCACCTTAAAAATGGAGATGCTCAGTGCCAAAACTCCGGATATGGTGCGCAAGGACATTTGGGTACATTTGT
TGGGCTATAACCTACTCAGAAGTCTCATGGAACTTGCGGCACCGCTAGCAGATAATGCTAGAACTCAACTGTCTGTGCAA
GGAGCACGACAACACTTCAATCAGATGCTTGCTTTGTTGGCGACAGCCAACCGTGCGACCAGAAAGCGGTTGTTTACTCA
TCTACTTGAGACCATGGCAGCCGATCTATTACCCTCTCGACCGAATCGGCACGAACCGAGAGTCGTCAAACGCAGACCCA
AATCTTTCCCGCGAATGCGACAACCTCGCTCTGCTCTCAAAGCTAATACCAAATCCGATTTATATAACCCCGATATGATG
CATTATTAG

Upstream 100 bases:

>100_bases
AAAATCCGGAAAGCAATATCTGCCCTGAATAACGTTAGCCGTGAGAGCTATTTAAGAATCAATGGCACTGACTTAAGAAG
TCAGGAAAAGGCTACAAGTC

Downstream 100 bases:

>100_bases
AGAATGCCAAGAAAACTATATCTCGAACCTCATTTTTCGCCTGACGAGCTGAAGTCTCATTATCGAGCCAGCAAAGACCC
AGTAGAATCGCGCCGCTGGC

Product: IS4 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHFNQMLALLATANRATRKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKANTKSDLYNPDMM
HY

Sequences:

>Translated_482_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHFNQMLALLATANRATRKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKANTKSDLYNPDMM
HY
>Mature_482_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHFNQMLALLATANRATRKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKANTKSDLYNPDMM
HY

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=357, Percent_Identity=25.4901960784314, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 54599; Mature: 54599

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNR
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHH
IYSPIVTLWAMLYQVLSADKSLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSAANQASYPQHGNQTAGCGFPI
HHHCCCHHHHHHCCCCCCCEECCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
ARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
LRLSLKGFRDQHIIVVTTLLDAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAK
HHHHHCCCCCCCEEEEEEHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
TPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQGARQHFNQMLALLATANRAT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKANTKSDLYNPDMM
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHCCCHHHHHCCCHHCCCCCCCC
HY
CC
>Mature Secondary Structure
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNR
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHH
IYSPIVTLWAMLYQVLSADKSLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSAANQASYPQHGNQTAGCGFPI
HHHCCCHHHHHHCCCCCCCEECCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
ARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
LRLSLKGFRDQHIIVVTTLLDAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAK
HHHHHCCCCCCCEEEEEEHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
TPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQGARQHFNQMLALLATANRAT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKANTKSDLYNPDMM
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHCCCHHHHHCCCHHCCCCCCCC
HY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]