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The map label for this gene is fliA [H]

Identifier: 158333719

GI number: 158333719

Start: 501625

End: 502419

Strand: Reverse

Name: fliA [H]

Synonym: AM1_0525

Alternate gene names: 158333719

Gene position: 502419-501625 (Counterclockwise)

Preceding gene: 158333720

Following gene: 158333716

Centisome position: 7.73

GC content: 45.41

Gene sequence:

>795_bases
ATGACCGCAACTCTTACTAATAATCTTAAAAGTGAAAGCTTGGAGTTATTGCAGCAGTATCAACAGTCTTCATGCACTGA
GTTGCGAAATCGCCTCGTACAGATGAATTTGGGATTAGTCAGGAGAGAGGCTCATCGCTGGGTTGGCCATTCCACTGAAA
GTTTTGATGATTTAATGCAGGTAGGTTCCCTTGGCTTAATTCGAGCCATCGAACGGTTTGACCCCTTTAAAGGACATGCC
TTTAGTTCCTTTGCAGTTCCCTACATCCGAGGAGAGATTCAACATTATCTGCGAGATAAAAGTCCTCAGGTTCGGATCCC
TCGCAAATGGCACGAACTCCAACGGCAGGCCATTAACGTGACCCGGGAAATGAGGGATGAATTACAACGGCAACCTACGG
ACGTTGAGGTAGCTGCAGCCTTAGACATTTGCCAAAAGGAATGGCAAGAAGTAAAGCTGGCATCTTGCAATCGCTCAGTT
TTAAGCCTTGATGCTCCAGTTCATAATTCTGAGCAAGAATCCATGTCTTTGGGTGAGCTACTGCCTGATCGCCACTATCA
TAGTTTTCAGCTCGCTCAAGAAGATCAAATTCGATTGCGCCAAGCCTTATCTAAGTTAGAAAAGCGAACTTCGGATGTCC
TAGAGTTTGTCTTTTTCCATGACTTAACTCAAAAGGAAACAGCTGAAGTCTTGGGTATCAGTGCGGTGACGGTCTCTCGT
CAAGTCAAGAAAGGGTTAGTTGCTCTCCGCCAAATGTTGAAAGGCTCTGAAGAGACAGCAGAGAAAACCGTTTAG

Upstream 100 bases:

>100_bases
TCCTTTGACTTCTTAAGTTAATGAAAGGTTCGAAATCGTTTTCTTCTCCTCAGAACTCTGAATTAGAACGGACTTAAAAA
CAAATTCATATTTTTTTGAC

Downstream 100 bases:

>100_bases
ATTCCCTAGCTTTTAATCTAACGGGGCTTGATCTTTGCTGTGCTGGGGTATGATGCCAAAGTTGTAAGCAAGAGCCAAGA
TTGGCGGTGTGATTAAGCTT

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTATLTNNLKSESLELLQQYQQSSCTELRNRLVQMNLGLVRREAHRWVGHSTESFDDLMQVGSLGLIRAIERFDPFKGHA
FSSFAVPYIRGEIQHYLRDKSPQVRIPRKWHELQRQAINVTREMRDELQRQPTDVEVAAALDICQKEWQEVKLASCNRSV
LSLDAPVHNSEQESMSLGELLPDRHYHSFQLAQEDQIRLRQALSKLEKRTSDVLEFVFFHDLTQKETAEVLGISAVTVSR
QVKKGLVALRQMLKGSEETAEKTV

Sequences:

>Translated_264_residues
MTATLTNNLKSESLELLQQYQQSSCTELRNRLVQMNLGLVRREAHRWVGHSTESFDDLMQVGSLGLIRAIERFDPFKGHA
FSSFAVPYIRGEIQHYLRDKSPQVRIPRKWHELQRQAINVTREMRDELQRQPTDVEVAAALDICQKEWQEVKLASCNRSV
LSLDAPVHNSEQESMSLGELLPDRHYHSFQLAQEDQIRLRQALSKLEKRTSDVLEFVFFHDLTQKETAEVLGISAVTVSR
QVKKGLVALRQMLKGSEETAEKTV
>Mature_263_residues
TATLTNNLKSESLELLQQYQQSSCTELRNRLVQMNLGLVRREAHRWVGHSTESFDDLMQVGSLGLIRAIERFDPFKGHAF
SSFAVPYIRGEIQHYLRDKSPQVRIPRKWHELQRQAINVTREMRDELQRQPTDVEVAAALDICQKEWQEVKLASCNRSVL
SLDAPVHNSEQESMSLGELLPDRHYHSFQLAQEDQIRLRQALSKLEKRTSDVLEFVFFHDLTQKETAEVLGISAVTVSRQ
VKKGLVALRQMLKGSEETAEKTV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:
GO:0003677: RNA polymerase sigma-24 subunit
GO:0003700: RNA polymerase sigma-24 subunit
GO:0006351: RNA polymerase sigma-24 subunit
GO:0006352: RNA polymerase sigma-24 subunit
GO:0006355: RNA polymerase sigma-24 subunit
GO:0016987: RNA polymerase sigma-24 subunit

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=232, Percent_Identity=31.4655172413793, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789098, Length=233, Percent_Identity=26.6094420600858, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789448, Length=208, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30396; Mature: 30265

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLTNNLKSESLELLQQYQQSSCTELRNRLVQMNLGLVRREAHRWVGHSTESFDDLMQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VGSLGLIRAIERFDPFKGHAFSSFAVPYIRGEIQHYLRDKSPQVRIPRKWHELQRQAINV
HCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
TREMRDELQRQPTDVEVAAALDICQKEWQEVKLASCNRSVLSLDAPVHNSEQESMSLGEL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHH
LPDRHYHSFQLAQEDQIRLRQALSKLEKRTSDVLEFVFFHDLTQKETAEVLGISAVTVSR
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVKKGLVALRQMLKGSEETAEKTV
HHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
TATLTNNLKSESLELLQQYQQSSCTELRNRLVQMNLGLVRREAHRWVGHSTESFDDLMQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VGSLGLIRAIERFDPFKGHAFSSFAVPYIRGEIQHYLRDKSPQVRIPRKWHELQRQAINV
HCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
TREMRDELQRQPTDVEVAAALDICQKEWQEVKLASCNRSVLSLDAPVHNSEQESMSLGEL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHH
LPDRHYHSFQLAQEDQIRLRQALSKLEKRTSDVLEFVFFHDLTQKETAEVLGISAVTVSR
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVKKGLVALRQMLKGSEETAEKTV
HHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]