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The map label for this gene is lip2 [H]

Identifier: 158333718

GI number: 158333718

Start: 499618

End: 500607

Strand: Direct

Name: lip2 [H]

Synonym: AM1_0524

Alternate gene names: 158333718

Gene position: 499618-500607 (Clockwise)

Preceding gene: 158333717

Following gene: 158333721

Centisome position: 7.68

GC content: 51.52

Gene sequence:

>990_bases
TTGGACTGGAAACTCCAGATTCTGAAGGGTTTATTAAACCGTTCTCAGCCGATTGAGCTGTTAAGTCCTGAAGCACTACG
CCAGCGGAATGAAACATCGCGCCCGGCTGTCATTGATTGGCTCAAGCATGGGCGCAAACGCAAATTACCCAAAGTTCGCA
ATCAGTGGGTGACGGGTCGCCATGGCAATATCCCTATTCGGCTTTATTACCCCAGCTTGAACTCCCCTCTCCCCTGCGTT
GTTTTCTTTCATGGTGGGGGTTGGGTAACGGGGAACCTGGGCACCCATGATGCCTTCTGTCGGCAGATTGCCTATCAAAG
TGGGGCTTTGGTCCTGTCGGTGGCCTATCGCTTAGCCCCTGAATTTCCCTATCCCACTCCTTTAGAAGATTGCTATGACG
CGACCCAATGGGCAGCTCAAAATGCGGATGCTTTAGGAGCTGATCCCCGGCAACTCATGGTTATGGGAGATAGTGCGGGA
GGCAACCTGGCTGCAGCTGTCTGTCTAATGGCTCGCGATTTAGAGGGTCCTAATCTCCAAAAACAGATTTTGCTCTATCC
AGCGTTGGATGGCACTCTCAACCATCCTTCCATGGATCAGTATGCGGATGCCCCCGTCCTCAAGAAAACTGCCATGGAGA
TCTTCATTAATCAATATGCCAATTCGCCTGCAGATATTCAGTCCCCCTATTTTTCGCCATTGTTAGCTGAGACCTTGAAT
CACTTACCGTCTGCCCTGGTAATTACGGCGGCCTATGATCCCTTACGAGATGAAGGCCAAGCCTATGCTCAGCGCTTACA
GCAGGCTGGTGTTCCCACCCAAGTGACGGACTATCCAGGCATGGTTCATGGTTTTCTGAGTTTTCCCCGATTCTGTAGCG
GTGCAAAGCTAGCCTGGGCTGAAATTACCCAATACCTTCAGGCCGCCACTTCTCCCAATTGTTCCCCTACCGATAGCCTT
AATCCATTGGCAAAGTCTCCCCCCGACTGA

Upstream 100 bases:

>100_bases
TTTGGGGGGTCTTTCCACTGCGCCACTTTGGATATTCGTCGGCGTGGTACTTTGCAGTCCTACTGTTAGGCCCATGCAGT
CTTCAGCACAGAAGTATCGA

Downstream 100 bases:

>100_bases
CGACGTTTACCGGCCATTTGCAAGGATCGCAATAGCAATAACCCTTCACCCGTTTGTACGACTGGACCTTGCTGAGGCGC
AATATAAACGATGGTACCGG

Product: lipase/esterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MDWKLQILKGLLNRSQPIELLSPEALRQRNETSRPAVIDWLKHGRKRKLPKVRNQWVTGRHGNIPIRLYYPSLNSPLPCV
VFFHGGGWVTGNLGTHDAFCRQIAYQSGALVLSVAYRLAPEFPYPTPLEDCYDATQWAAQNADALGADPRQLMVMGDSAG
GNLAAAVCLMARDLEGPNLQKQILLYPALDGTLNHPSMDQYADAPVLKKTAMEIFINQYANSPADIQSPYFSPLLAETLN
HLPSALVITAAYDPLRDEGQAYAQRLQQAGVPTQVTDYPGMVHGFLSFPRFCSGAKLAWAEITQYLQAATSPNCSPTDSL
NPLAKSPPD

Sequences:

>Translated_329_residues
MDWKLQILKGLLNRSQPIELLSPEALRQRNETSRPAVIDWLKHGRKRKLPKVRNQWVTGRHGNIPIRLYYPSLNSPLPCV
VFFHGGGWVTGNLGTHDAFCRQIAYQSGALVLSVAYRLAPEFPYPTPLEDCYDATQWAAQNADALGADPRQLMVMGDSAG
GNLAAAVCLMARDLEGPNLQKQILLYPALDGTLNHPSMDQYADAPVLKKTAMEIFINQYANSPADIQSPYFSPLLAETLN
HLPSALVITAAYDPLRDEGQAYAQRLQQAGVPTQVTDYPGMVHGFLSFPRFCSGAKLAWAEITQYLQAATSPNCSPTDSL
NPLAKSPPD
>Mature_329_residues
MDWKLQILKGLLNRSQPIELLSPEALRQRNETSRPAVIDWLKHGRKRKLPKVRNQWVTGRHGNIPIRLYYPSLNSPLPCV
VFFHGGGWVTGNLGTHDAFCRQIAYQSGALVLSVAYRLAPEFPYPTPLEDCYDATQWAAQNADALGADPRQLMVMGDSAG
GNLAAAVCLMARDLEGPNLQKQILLYPALDGTLNHPSMDQYADAPVLKKTAMEIFINQYANSPADIQSPYFSPLLAETLN
HLPSALVITAAYDPLRDEGQAYAQRLQQAGVPTQVTDYPGMVHGFLSFPRFCSGAKLAWAEITQYLQAATSPNCSPTDSL
NPLAKSPPD

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=299, Percent_Identity=29.7658862876254, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI68299767, Length=315, Percent_Identity=27.9365079365079, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI157041237, Length=264, Percent_Identity=27.6515151515151, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI61966717, Length=291, Percent_Identity=24.3986254295533, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI206597554, Length=289, Percent_Identity=28.719723183391, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI21328446, Length=92, Percent_Identity=41.304347826087, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI68051721, Length=234, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI226423947, Length=228, Percent_Identity=26.7543859649123, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=259, Percent_Identity=28.1853281853282, Blast_Score=108, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17567059, Length=266, Percent_Identity=30.8270676691729, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI72001146, Length=149, Percent_Identity=37.5838926174497, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17540028, Length=130, Percent_Identity=36.9230769230769, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71996133, Length=130, Percent_Identity=35.3846153846154, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI115533410, Length=103, Percent_Identity=37.8640776699029, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=103, Percent_Identity=37.8640776699029, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24656084, Length=95, Percent_Identity=38.9473684210526, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656076, Length=95, Percent_Identity=38.9473684210526, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130169, Length=95, Percent_Identity=38.9473684210526, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 36200; Mature: 36200

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWKLQILKGLLNRSQPIELLSPEALRQRNETSRPAVIDWLKHGRKRKLPKVRNQWVTGR
CCHHHHHHHHHHCCCCCCHHCCCHHHHHCCCCCCHHHHHHHHCCHHCCCHHHHHHCCCCC
HGNIPIRLYYPSLNSPLPCVVFFHGGGWVTGNLGTHDAFCRQIAYQSGALVLSVAYRLAP
CCCEEEEEECCCCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCC
EFPYPTPLEDCYDATQWAAQNADALGADPRQLMVMGDSAGGNLAAAVCLMARDLEGPNLQ
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
KQILLYPALDGTLNHPSMDQYADAPVLKKTAMEIFINQYANSPADIQSPYFSPLLAETLN
CEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
HLPSALVITAAYDPLRDEGQAYAQRLQQAGVPTQVTDYPGMVHGFLSFPRFCSGAKLAWA
HCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCHHHH
EITQYLQAATSPNCSPTDSLNPLAKSPPD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDWKLQILKGLLNRSQPIELLSPEALRQRNETSRPAVIDWLKHGRKRKLPKVRNQWVTGR
CCHHHHHHHHHHCCCCCCHHCCCHHHHHCCCCCCHHHHHHHHCCHHCCCHHHHHHCCCCC
HGNIPIRLYYPSLNSPLPCVVFFHGGGWVTGNLGTHDAFCRQIAYQSGALVLSVAYRLAP
CCCEEEEEECCCCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCC
EFPYPTPLEDCYDATQWAAQNADALGADPRQLMVMGDSAGGNLAAAVCLMARDLEGPNLQ
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
KQILLYPALDGTLNHPSMDQYADAPVLKKTAMEIFINQYANSPADIQSPYFSPLLAETLN
CEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
HLPSALVITAAYDPLRDEGQAYAQRLQQAGVPTQVTDYPGMVHGFLSFPRFCSGAKLAWA
HCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCHHHH
EITQYLQAATSPNCSPTDSLNPLAKSPPD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]