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The map label for this gene is hemN [H]

Identifier: 158333665

GI number: 158333665

Start: 444729

End: 446123

Strand: Direct

Name: hemN [H]

Synonym: AM1_0467

Alternate gene names: 158333665

Gene position: 444729-446123 (Clockwise)

Preceding gene: 158333664

Following gene: 158333666

Centisome position: 6.84

GC content: 47.74

Gene sequence:

>1395_bases
ATGGAATTACTGTCTACAGTTCGCTGCGATCTCGGATTACTGAATAAGTATGATCAACCCTTGCCGCGCTATACCAGCTA
TCCACCCGCCACAGAATTTAGCTCCGAATTGACCCCTGAGCGGTTCCAGCAAGCCCTGGCAAAGGGAAATGATCAGCAAA
TTCCCCTATCCCTTTATTGCCATATTCCTTTTTGCGAAACCCCTTGCTTTTTTTGTGGGTGCAATACGGTGATCACTCAG
CGTAAACAGGTGGCCGATCCCTATCTGCAATACCTGATCCGGAATATTCGGCAAGTGGCTGAACAAGTGGGAAGCGATCG
CAACGTTCACCAACTCCACTGGGGGGGAGGAACGCCTAATTATTTAAGTTGGCCCCAAATTGAGCTGCTCTGGAATGCCT
TGCAAGATGCCTTCTCTTTCGATCCGGCGGCAGAAATATCTATTGAGGTGAATCCCTGCTATATCGATCGCAACACGGTA
TTTTTTCTGCGAGAGCTGGGGTTCAACCGGATTAGCTTTGGCATTCAAGATTTTGATCCGCAGGTTCAGATTGCTGTCAA
CCGGATTCAGTCTGAGGAACTGCTGTTTAACGTGATCAGCTGGATTCAAGAAGCGGACTTTGACAGTGTAAATGTGGACT
TAATCTATGGCTTGCCCCATCAGACGGTGACGACCTTTGCCAACACAGTACAAAAAACGATTCAGCTTAATCCTGACCGG
ATTGCTGTGTTCAACTTTGCTTATATGCCCCAGTTAATCCCCGTCCAGCGCCAATTGCCTGCCCATGCCCTTCCCCACAA
AGCAGAAAAGCTAGAGATGTTGCAGCAGACCATCGACCAGCTGGAGGGCAATGGATATCGCTTTATTGGGATGGATCATT
TTGCTAAACCCAAGGATGAGTTGGCTTTAGCCCAAACAGCAGGACAGCTACACCGAAACTTTCAGGGATATACGACTAAA
CCGGAGTCGGATTTACTGGGTTTTGGGCTCTCGGCCATCAGTATGCTCCAGGATGTTTATACCCAAAACCATAAACGCCT
CAAGGATTATTACCATGCCTTAGACACCACCCCATTCCCCATTGCCAAAGGGGTGGGGTTAAGTGAGGATGATCAGGTGC
GTCGAGCTGTGATTATGGAACTGATGTGCCAGTTTCAGCTGTCGCAGGTAGCGCTTGAAGAAAAGTACCAGTTGAAATTT
GACCCAGATTTTGAGCAATATTTCGCATCTGAAAAGACTGCCCTGCAAGCGTTGGCAGCAGATGGTCTGGTGGAGTTATC
TCCTAATCAGATTCAGGTGACACCCACTGGACGCTTACTGATTCGCAATATTGCAGCGGTATTCGATATCTATTTACAAT
CTCACGCTGATCAACGTTTCTCACAATCGATTTAG

Upstream 100 bases:

>100_bases
TGATTGACAGGAAGCAAACCGTGACATACCAGACCCGCCGCCCATTGTTGGGAGAGGTGGGCTACCATCCCCCACTATTA
CTGAATAAGGTCAGTCCCCT

Downstream 100 bases:

>100_bases
CGAACTATACCGATTGGATTTCAGAGAGGCTGGTGCTTTCAGGAGACATGGCGCTAATATGAGCTGATCCAGCGTTTGGA
CCCTCATAAATGGCATCTCA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MELLSTVRCDLGLLNKYDQPLPRYTSYPPATEFSSELTPERFQQALAKGNDQQIPLSLYCHIPFCETPCFFCGCNTVITQ
RKQVADPYLQYLIRNIRQVAEQVGSDRNVHQLHWGGGTPNYLSWPQIELLWNALQDAFSFDPAAEISIEVNPCYIDRNTV
FFLRELGFNRISFGIQDFDPQVQIAVNRIQSEELLFNVISWIQEADFDSVNVDLIYGLPHQTVTTFANTVQKTIQLNPDR
IAVFNFAYMPQLIPVQRQLPAHALPHKAEKLEMLQQTIDQLEGNGYRFIGMDHFAKPKDELALAQTAGQLHRNFQGYTTK
PESDLLGFGLSAISMLQDVYTQNHKRLKDYYHALDTTPFPIAKGVGLSEDDQVRRAVIMELMCQFQLSQVALEEKYQLKF
DPDFEQYFASEKTALQALAADGLVELSPNQIQVTPTGRLLIRNIAAVFDIYLQSHADQRFSQSI

Sequences:

>Translated_464_residues
MELLSTVRCDLGLLNKYDQPLPRYTSYPPATEFSSELTPERFQQALAKGNDQQIPLSLYCHIPFCETPCFFCGCNTVITQ
RKQVADPYLQYLIRNIRQVAEQVGSDRNVHQLHWGGGTPNYLSWPQIELLWNALQDAFSFDPAAEISIEVNPCYIDRNTV
FFLRELGFNRISFGIQDFDPQVQIAVNRIQSEELLFNVISWIQEADFDSVNVDLIYGLPHQTVTTFANTVQKTIQLNPDR
IAVFNFAYMPQLIPVQRQLPAHALPHKAEKLEMLQQTIDQLEGNGYRFIGMDHFAKPKDELALAQTAGQLHRNFQGYTTK
PESDLLGFGLSAISMLQDVYTQNHKRLKDYYHALDTTPFPIAKGVGLSEDDQVRRAVIMELMCQFQLSQVALEEKYQLKF
DPDFEQYFASEKTALQALAADGLVELSPNQIQVTPTGRLLIRNIAAVFDIYLQSHADQRFSQSI
>Mature_464_residues
MELLSTVRCDLGLLNKYDQPLPRYTSYPPATEFSSELTPERFQQALAKGNDQQIPLSLYCHIPFCETPCFFCGCNTVITQ
RKQVADPYLQYLIRNIRQVAEQVGSDRNVHQLHWGGGTPNYLSWPQIELLWNALQDAFSFDPAAEISIEVNPCYIDRNTV
FFLRELGFNRISFGIQDFDPQVQIAVNRIQSEELLFNVISWIQEADFDSVNVDLIYGLPHQTVTTFANTVQKTIQLNPDR
IAVFNFAYMPQLIPVQRQLPAHALPHKAEKLEMLQQTIDQLEGNGYRFIGMDHFAKPKDELALAQTAGQLHRNFQGYTTK
PESDLLGFGLSAISMLQDVYTQNHKRLKDYYHALDTTPFPIAKGVGLSEDDQVRRAVIMELMCQFQLSQVALEEKYQLKF
DPDFEQYFASEKTALQALAADGLVELSPNQIQVTPTGRLLIRNIAAVFDIYLQSHADQRFSQSI

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:
GO:0003824: Oxygen-independent coproporphyrinogen III oxidase
GO:0004109: Oxygen-independent coproporphyrinogen III oxidase
GO:0005737: Oxygen-independent coproporphyrinogen III oxidase
GO:0006779: Oxygen-independent coproporphyrinogen III oxidase
GO:0051536: Oxygen-independent coproporphyrinogen III oxidase
GO:0055114: Oxygen-independent coproporphyrinogen III oxidase

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=242, Percent_Identity=24.3801652892562, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87082341, Length=456, Percent_Identity=48.9035087719298, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1789325, Length=244, Percent_Identity=28.2786885245902, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 52958; Mature: 52958

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLSTVRCDLGLLNKYDQPLPRYTSYPPATEFSSELTPERFQQALAKGNDQQIPLSLYC
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
HIPFCETPCFFCGCNTVITQRKQVADPYLQYLIRNIRQVAEQVGSDRNVHQLHWGGGTPN
ECCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
YLSWPQIELLWNALQDAFSFDPAAEISIEVNPCYIDRNTVFFLRELGFNRISFGIQDFDP
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECEEEECCCHHHHHHHHCCCCEECCCCCCCC
QVQIAVNRIQSEELLFNVISWIQEADFDSVNVDLIYGLPHQTVTTFANTVQKTIQLNPDR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCE
IAVFNFAYMPQLIPVQRQLPAHALPHKAEKLEMLQQTIDQLEGNGYRFIGMDHFAKPKDE
EEEEEHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHH
LALAQTAGQLHRNFQGYTTKPESDLLGFGLSAISMLQDVYTQNHKRLKDYYHALDTTPFP
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IAKGVGLSEDDQVRRAVIMELMCQFQLSQVALEEKYQLKFDPDFEQYFASEKTALQALAA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCHHHHHHHHHH
DGLVELSPNQIQVTPTGRLLIRNIAAVFDIYLQSHADQRFSQSI
CCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELLSTVRCDLGLLNKYDQPLPRYTSYPPATEFSSELTPERFQQALAKGNDQQIPLSLYC
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
HIPFCETPCFFCGCNTVITQRKQVADPYLQYLIRNIRQVAEQVGSDRNVHQLHWGGGTPN
ECCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
YLSWPQIELLWNALQDAFSFDPAAEISIEVNPCYIDRNTVFFLRELGFNRISFGIQDFDP
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECEEEECCCHHHHHHHHCCCCEECCCCCCCC
QVQIAVNRIQSEELLFNVISWIQEADFDSVNVDLIYGLPHQTVTTFANTVQKTIQLNPDR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCE
IAVFNFAYMPQLIPVQRQLPAHALPHKAEKLEMLQQTIDQLEGNGYRFIGMDHFAKPKDE
EEEEEHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHH
LALAQTAGQLHRNFQGYTTKPESDLLGFGLSAISMLQDVYTQNHKRLKDYYHALDTTPFP
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IAKGVGLSEDDQVRRAVIMELMCQFQLSQVALEEKYQLKFDPDFEQYFASEKTALQALAA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCHHHHHHHHHH
DGLVELSPNQIQVTPTGRLLIRNIAAVFDIYLQSHADQRFSQSI
CCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]