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The map label for this gene is asnB [H]

Identifier: 158333577

GI number: 158333577

Start: 346827

End: 348638

Strand: Reverse

Name: asnB [H]

Synonym: AM1_0378

Alternate gene names: 158333577

Gene position: 348638-346827 (Counterclockwise)

Preceding gene: 158333578

Following gene: 158333576

Centisome position: 5.36

GC content: 39.46

Gene sequence:

>1812_bases
ATGTTACCAAGATGCAATAAGAATTGCAGTAGGTTAAAACGTATGTCTGAGATTACGGGTTGTTTATCTATAGAGGAAGA
AGTCCATTCATCACCTTTAACTTATTCATCATGTAAGAATTCATCATACAAGAGTAGGATTCCTGGAGAATCTTCTTGGA
CTAGTCAAGATCGCTACTGCTGGCTTCAATACAGTCGAAACTTATCAAATCAACAGAACGATGTTGCATCTCAGCCTATT
CGGTCCCATTGTGGTCGGTATGTACTTGTCTTTAGTGGACACATCAATAATCATTTAGAAATTCGTCGAGGACTTCGGAA
TCAGATCTGGAAGGGAAATAGTGATGTTGAAACTCTGGTTGAAGGCTTAGCTCAACGAAATACTTCACTCTTAGTTGACC
TGAGAGGAGTCTTTGCCTTTGCTTTCTACGATACTGAGACTAGACAGTTGCTACTGGCTCGAGATCGCCTTGGTGTTAAA
TCTCTTTGGTGGCAAAAACAAGACACGAAGCTGTGTTTTTCTTCTAATGTACGTCAATTATTCCAGGATAAGCTTAAACT
AGCACCTTCCTGTGTTAGCCATTATCTTAGTTTTGGATACTATCCAATGGAAGGTGAATTAGGTGAGGGGATTTACCCTC
TTCCTCCTGGAACCCTTATGAGAATTGGACCTGACTCTTCGCCAACTTTTTTGCGTTGGTGGCCCCCGTCTCCCCGACCA
GATTGGACTCCTTTACCTATCAAACGACGTCGTGATGCGGTGAAGCTGATTCGGCAAGAACTTGAGCGAGTAATTCAAGA
ACATATTTCCAGTGATAGACCTATTAATTGCTTGCTCACGAACGATTCCAGCTCCAAAATCGTTGCAGCTCTTGCTGCTC
AGCATAGTTCTAAGCCAATTAAAACATTCTCAGTACATATCCAAGACTCTTCTATACCAAAAAATGGTATTACGGATTTT
GCAAACCACATCGGAGCATTTCATCAAGAAATTTATATCAGTAGTAAAGAATATATCGAATTTTTTACTAAGGCTATTAA
GACTCTAGAATTACCAAGTTTCCAAGCTGCTCATAGTTGTGTGATTGGAAATGCATTAAAGCGATCAGGTGTCCAAACGA
TAATGTTTGGTTTTGAAAGTCAAAAAGCATTAGGAGTTCACCTAAGTGGATTAGTTTTGATGGCACGACTTGCCAGATTA
AGAGTTTTTGAATTGTGGCCAAAGGTTATGCAGGTAATGAATTCCCGTTTATTTTCTATGGCTGAAGATGCTCCTTGGTT
AGATGCGTGGCATCTCCTCTTGGCAGGCCATCGCCTTGCCACTAATGCAGAGTTAAAAACGACCAATTTACAGGCTCTTG
ATTGGCCAAAGCCCCCATCGTGTCGACTTACACAATTTTTACCAAGAATCTTTTGGAACGATTTGTTTGGATATGTAGAG
CCGTTTCTATTGCGGGACATTGATCAAATGGAACGAATAGTTGGGATAGAAACAAAACTACCGTTATTTGACCATCGACT
TCTTGAACTTTTACTAAGAATTCCAGAAGCATTCTATCTAATGTCCTGGGATCGATCTTTGCTGGAAATTGCATGCCAAG
ACTTATTGCCTAGGTGGATAATGAGATCGAAGATGTCGCTCAAAAATGTATCATTGACAGGAAAACATATGGAAAAATTA
CAGTCTGAAGTTATCTTGTCAATATTTAACGAGGTTAATCCCATACCAACACAAATATTAAACAAAGAAAGGAATATAGA
TTCTTTGTTATATCAGAATAATTTACCACTCATCACATCAAACTTGGGTTGA

Upstream 100 bases:

>100_bases
TTTTAACAATACCTTCTGATTTAAAAGAGGCTAAGAAAAAAGAAAGGATCAACCATGCAGGACGTTTCTCGCTTGAGCAT
TCTTTACTTGAATATGAACG

Downstream 100 bases:

>100_bases
TTATAGGTTCACTAAAGAGCAGCTCAATAGTTAAACTTAAATATGAAAATTCTGAATGTAATTTCTTCAATTAACCCAGC
TGGTGGGGGGCCAATTGAGA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MLPRCNKNCSRLKRMSEITGCLSIEEEVHSSPLTYSSCKNSSYKSRIPGESSWTSQDRYCWLQYSRNLSNQQNDVASQPI
RSHCGRYVLVFSGHINNHLEIRRGLRNQIWKGNSDVETLVEGLAQRNTSLLVDLRGVFAFAFYDTETRQLLLARDRLGVK
SLWWQKQDTKLCFSSNVRQLFQDKLKLAPSCVSHYLSFGYYPMEGELGEGIYPLPPGTLMRIGPDSSPTFLRWWPPSPRP
DWTPLPIKRRRDAVKLIRQELERVIQEHISSDRPINCLLTNDSSSKIVAALAAQHSSKPIKTFSVHIQDSSIPKNGITDF
ANHIGAFHQEIYISSKEYIEFFTKAIKTLELPSFQAAHSCVIGNALKRSGVQTIMFGFESQKALGVHLSGLVLMARLARL
RVFELWPKVMQVMNSRLFSMAEDAPWLDAWHLLLAGHRLATNAELKTTNLQALDWPKPPSCRLTQFLPRIFWNDLFGYVE
PFLLRDIDQMERIVGIETKLPLFDHRLLELLLRIPEAFYLMSWDRSLLEIACQDLLPRWIMRSKMSLKNVSLTGKHMEKL
QSEVILSIFNEVNPIPTQILNKERNIDSLLYQNNLPLITSNLG

Sequences:

>Translated_603_residues
MLPRCNKNCSRLKRMSEITGCLSIEEEVHSSPLTYSSCKNSSYKSRIPGESSWTSQDRYCWLQYSRNLSNQQNDVASQPI
RSHCGRYVLVFSGHINNHLEIRRGLRNQIWKGNSDVETLVEGLAQRNTSLLVDLRGVFAFAFYDTETRQLLLARDRLGVK
SLWWQKQDTKLCFSSNVRQLFQDKLKLAPSCVSHYLSFGYYPMEGELGEGIYPLPPGTLMRIGPDSSPTFLRWWPPSPRP
DWTPLPIKRRRDAVKLIRQELERVIQEHISSDRPINCLLTNDSSSKIVAALAAQHSSKPIKTFSVHIQDSSIPKNGITDF
ANHIGAFHQEIYISSKEYIEFFTKAIKTLELPSFQAAHSCVIGNALKRSGVQTIMFGFESQKALGVHLSGLVLMARLARL
RVFELWPKVMQVMNSRLFSMAEDAPWLDAWHLLLAGHRLATNAELKTTNLQALDWPKPPSCRLTQFLPRIFWNDLFGYVE
PFLLRDIDQMERIVGIETKLPLFDHRLLELLLRIPEAFYLMSWDRSLLEIACQDLLPRWIMRSKMSLKNVSLTGKHMEKL
QSEVILSIFNEVNPIPTQILNKERNIDSLLYQNNLPLITSNLG
>Mature_603_residues
MLPRCNKNCSRLKRMSEITGCLSIEEEVHSSPLTYSSCKNSSYKSRIPGESSWTSQDRYCWLQYSRNLSNQQNDVASQPI
RSHCGRYVLVFSGHINNHLEIRRGLRNQIWKGNSDVETLVEGLAQRNTSLLVDLRGVFAFAFYDTETRQLLLARDRLGVK
SLWWQKQDTKLCFSSNVRQLFQDKLKLAPSCVSHYLSFGYYPMEGELGEGIYPLPPGTLMRIGPDSSPTFLRWWPPSPRP
DWTPLPIKRRRDAVKLIRQELERVIQEHISSDRPINCLLTNDSSSKIVAALAAQHSSKPIKTFSVHIQDSSIPKNGITDF
ANHIGAFHQEIYISSKEYIEFFTKAIKTLELPSFQAAHSCVIGNALKRSGVQTIMFGFESQKALGVHLSGLVLMARLARL
RVFELWPKVMQVMNSRLFSMAEDAPWLDAWHLLLAGHRLATNAELKTTNLQALDWPKPPSCRLTQFLPRIFWNDLFGYVE
PFLLRDIDQMERIVGIETKLPLFDHRLLELLLRIPEAFYLMSWDRSLLEIACQDLLPRWIMRSKMSLKNVSLTGKHMEKL
QSEVILSIFNEVNPIPTQILNKERNIDSLLYQNNLPLITSNLG

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321563, Length=226, Percent_Identity=25.2212389380531, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325403, Length=286, Percent_Identity=22.027972027972, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69272; Mature: 69272

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPRCNKNCSRLKRMSEITGCLSIEEEVHSSPLTYSSCKNSSYKSRIPGESSWTSQDRYC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEE
WLQYSRNLSNQQNDVASQPIRSHCGRYVLVFSGHINNHLEIRRGLRNQIWKGNSDVETLV
EEEECCCCCCCHHHHHCCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHH
EGLAQRNTSLLVDLRGVFAFAFYDTETRQLLLARDRLGVKSLWWQKQDTKLCFSSNVRQL
HHHHHCCCEEEEEHHHHEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHH
FQDKLKLAPSCVSHYLSFGYYPMEGELGEGIYPLPPGTLMRIGPDSSPTFLRWWPPSPRP
HHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCC
DWTPLPIKRRRDAVKLIRQELERVIQEHISSDRPINCLLTNDSSSKIVAALAAQHSSKPI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCC
KTFSVHIQDSSIPKNGITDFANHIGAFHQEIYISSKEYIEFFTKAIKTLELPSFQAAHSC
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VIGNALKRSGVQTIMFGFESQKALGVHLSGLVLMARLARLRVFELWPKVMQVMNSRLFSM
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEDAPWLDAWHLLLAGHRLATNAELKTTNLQALDWPKPPSCRLTQFLPRIFWNDLFGYVE
HCCCCHHHHHHHHHHCCHHCCCCEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
PFLLRDIDQMERIVGIETKLPLFDHRLLELLLRIPEAFYLMSWDRSLLEIACQDLLPRWI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHH
MRSKMSLKNVSLTGKHMEKLQSEVILSIFNEVNPIPTQILNKERNIDSLLYQNNLPLITS
HHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHCCCCCEEEC
NLG
CCC
>Mature Secondary Structure
MLPRCNKNCSRLKRMSEITGCLSIEEEVHSSPLTYSSCKNSSYKSRIPGESSWTSQDRYC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEE
WLQYSRNLSNQQNDVASQPIRSHCGRYVLVFSGHINNHLEIRRGLRNQIWKGNSDVETLV
EEEECCCCCCCHHHHHCCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHH
EGLAQRNTSLLVDLRGVFAFAFYDTETRQLLLARDRLGVKSLWWQKQDTKLCFSSNVRQL
HHHHHCCCEEEEEHHHHEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHH
FQDKLKLAPSCVSHYLSFGYYPMEGELGEGIYPLPPGTLMRIGPDSSPTFLRWWPPSPRP
HHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCC
DWTPLPIKRRRDAVKLIRQELERVIQEHISSDRPINCLLTNDSSSKIVAALAAQHSSKPI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCC
KTFSVHIQDSSIPKNGITDFANHIGAFHQEIYISSKEYIEFFTKAIKTLELPSFQAAHSC
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VIGNALKRSGVQTIMFGFESQKALGVHLSGLVLMARLARLRVFELWPKVMQVMNSRLFSM
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEDAPWLDAWHLLLAGHRLATNAELKTTNLQALDWPKPPSCRLTQFLPRIFWNDLFGYVE
HCCCCHHHHHHHHHHCCHHCCCCEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
PFLLRDIDQMERIVGIETKLPLFDHRLLELLLRIPEAFYLMSWDRSLLEIACQDLLPRWI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHH
MRSKMSLKNVSLTGKHMEKLQSEVILSIFNEVNPIPTQILNKERNIDSLLYQNNLPLITS
HHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHCCCCCEEEC
NLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]