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The map label for this gene is glsA

Identifier: 158333566

GI number: 158333566

Start: 334454

End: 335419

Strand: Reverse

Name: glsA

Synonym: AM1_0365

Alternate gene names: 158333566

Gene position: 335419-334454 (Counterclockwise)

Preceding gene: 158333567

Following gene: 158333564

Centisome position: 5.16

GC content: 48.96

Gene sequence:

>966_bases
ATGTCGGAGTTACAAGTCCTTCTCGAATCCTTTCATCAAACCTACCATCAGCAAGATGCTGGACAACTGGCCTGCTATAT
TCCAGAACTGTGTAAAGCCGATCCCAATTGGTTTGCCATTTCTATCGTCACCACCGATGGTCAAACCTTCCAAGTGGGGA
ATTGTCAGCAAACCTTTACCATCCAATCCATTTCCAAGGTCTTTGTCTATGGTATGGCCCTTGCCGATCATGGCCAGGAC
GCCTTACTCGCAAAAGTAGGGGTTGAACCCACCGGAGATCCCTTCAATTCTCTGATTCGCCTAGACGAAGACTCCAAGCG
ACCCGACAATCCCATGATTAACGCGGGAGCGATCGCCACAACCAGCTTGATTCGAGGCAATGGCCCTACCGACCGCCTCA
ACCGATTGTTGGAGATGTTTCGCCGCTATGTGGGCCATGAAGTCTTTATTGATATGCCCGTATTTATGTCCGAGCGGGAT
ACCGGGCATCGTAACCGAGCTATTGCCCACTTAATGCTCAATTTCGGTATGATCGACAAGAATATCGATGAAGCCCTAGA
TCTCTATTTTCAGCAATGTTCAGTATTGGTCAATTGCCATGACTTAGCCGTGATGGCAGCCACTCTAGCCCATAAAGGGA
TTAATCCCATCACCCAAGAACGGGCCGTCGAATCCGGCTATGTCAAAGATATTCTTAGCGTCATGTACACCTGCGGCATG
TATGACTTTGCCGGGGAATGGGCCTATAAAGTCGGTTTACCTGCAAAGAGTGGCGTTTCTGGAGGGATTATGGCCGTGGT
CCCCAACCGAGCAGGCATTGCCGTATTTTCTCCTCTGCTGGATGAATATGGGAATAGCATTCGGGGCCGTCGAGTTCTAG
AAGCACTTTCTCAAAAACTAGGGTTGCACCTGTTCGATTTGTCGATGGGTGGATGTGGTTTACAAGCAGCAATGGACTCA
AATTAA

Upstream 100 bases:

>100_bases
TCGACAAATCAGAGAAACCGAATTTGCGCCAATGACCTCACTGATGTGATCGCACCCCGCAATATTTGCGACAATAGCTC
CAGACTTAGTAGGGGAATCA

Downstream 100 bases:

>100_bases
CCGCTTCGTCCACAAGCAGCTATTTTCAATAGACGTTGGAACCGATGTATCCAGTTTACGTATGGAATGATTAAATCAAG
ACTTTGCCATCCGAACGATA

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSELQVLLESFHQTYHQQDAGQLACYIPELCKADPNWFAISIVTTDGQTFQVGNCQQTFTIQSISKVFVYGMALADHGQD
ALLAKVGVEPTGDPFNSLIRLDEDSKRPDNPMINAGAIATTSLIRGNGPTDRLNRLLEMFRRYVGHEVFIDMPVFMSERD
TGHRNRAIAHLMLNFGMIDKNIDEALDLYFQQCSVLVNCHDLAVMAATLAHKGINPITQERAVESGYVKDILSVMYTCGM
YDFAGEWAYKVGLPAKSGVSGGIMAVVPNRAGIAVFSPLLDEYGNSIRGRRVLEALSQKLGLHLFDLSMGGCGLQAAMDS
N

Sequences:

>Translated_321_residues
MSELQVLLESFHQTYHQQDAGQLACYIPELCKADPNWFAISIVTTDGQTFQVGNCQQTFTIQSISKVFVYGMALADHGQD
ALLAKVGVEPTGDPFNSLIRLDEDSKRPDNPMINAGAIATTSLIRGNGPTDRLNRLLEMFRRYVGHEVFIDMPVFMSERD
TGHRNRAIAHLMLNFGMIDKNIDEALDLYFQQCSVLVNCHDLAVMAATLAHKGINPITQERAVESGYVKDILSVMYTCGM
YDFAGEWAYKVGLPAKSGVSGGIMAVVPNRAGIAVFSPLLDEYGNSIRGRRVLEALSQKLGLHLFDLSMGGCGLQAAMDS
N
>Mature_320_residues
SELQVLLESFHQTYHQQDAGQLACYIPELCKADPNWFAISIVTTDGQTFQVGNCQQTFTIQSISKVFVYGMALADHGQDA
LLAKVGVEPTGDPFNSLIRLDEDSKRPDNPMINAGAIATTSLIRGNGPTDRLNRLLEMFRRYVGHEVFIDMPVFMSERDT
GHRNRAIAHLMLNFGMIDKNIDEALDLYFQQCSVLVNCHDLAVMAATLAHKGINPITQERAVESGYVKDILSVMYTCGMY
DFAGEWAYKVGLPAKSGVSGGIMAVVPNRAGIAVFSPLLDEYGNSIRGRRVLEALSQKLGLHLFDLSMGGCGLQAAMDSN

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family

Homologues:

Organism=Homo sapiens, GI156104878, Length=303, Percent_Identity=40.9240924092409, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI20336214, Length=290, Percent_Identity=42.0689655172414, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1786693, Length=304, Percent_Identity=42.7631578947368, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1787804, Length=286, Percent_Identity=41.2587412587413, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17507019, Length=293, Percent_Identity=46.0750853242321, Blast_Score=258, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI193204073, Length=316, Percent_Identity=43.3544303797468, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI193204075, Length=318, Percent_Identity=43.0817610062893, Blast_Score=250, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17532727, Length=291, Percent_Identity=44.6735395189003, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI281363241, Length=307, Percent_Identity=41.3680781758958, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24653162, Length=307, Percent_Identity=41.3680781758958, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI281363239, Length=307, Percent_Identity=41.3680781758958, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24653164, Length=307, Percent_Identity=41.3680781758958, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24653156, Length=307, Percent_Identity=41.3680781758958, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24653158, Length=307, Percent_Identity=41.3680781758958, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI116008307, Length=307, Percent_Identity=41.3680781758958, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24653166, Length=307, Percent_Identity=41.3680781758958, Blast_Score=238, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLSA_ACAM1 (B0C9Y4)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001514738.1
- ProteinModelPortal:   B0C9Y4
- SMR:   B0C9Y4
- GeneID:   5679197
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0365
- HOGENOM:   HBG512335
- OMA:   LLENDHP
- ProtClustDB:   CLSK895844
- BioCyc:   AMAR329726:AM1_0365-MONOMER
- HAMAP:   MF_00313
- InterPro:   IPR012338
- InterPro:   IPR015868
- Gene3D:   G3DSA:3.40.710.20
- PANTHER:   PTHR12544
- TIGRFAMs:   TIGR03814

Pfam domain/function: PF04960 Glutaminase; SSF56601 PBP_transp_fold

EC number: =3.5.1.2

Molecular weight: Translated: 35262; Mature: 35131

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELQVLLESFHQTYHQQDAGQLACYIPELCKADPNWFAISIVTTDGQTFQVGNCQQTFT
CHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCEEEEEEEECCCCEEEECCCCHHHH
IQSISKVFVYGMALADHGQDALLAKVGVEPTGDPFNSLIRLDEDSKRPDNPMINAGAIAT
HHHHHHHHHHHHHHHCCCCCCEEHCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCHHHH
TSLIRGNGPTDRLNRLLEMFRRYVGHEVFIDMPVFMSERDTGHRNRAIAHLMLNFGMIDK
HHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHCCCCC
NIDEALDLYFQQCSVLVNCHDLAVMAATLAHKGINPITQERAVESGYVKDILSVMYTCGM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
YDFAGEWAYKVGLPAKSGVSGGIMAVVPNRAGIAVFSPLLDEYGNSIRGRRVLEALSQKL
HHHCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GLHLFDLSMGGCGLQAAMDSN
CCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SELQVLLESFHQTYHQQDAGQLACYIPELCKADPNWFAISIVTTDGQTFQVGNCQQTFT
HHHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCEEEEEEEECCCCEEEECCCCHHHH
IQSISKVFVYGMALADHGQDALLAKVGVEPTGDPFNSLIRLDEDSKRPDNPMINAGAIAT
HHHHHHHHHHHHHHHCCCCCCEEHCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCHHHH
TSLIRGNGPTDRLNRLLEMFRRYVGHEVFIDMPVFMSERDTGHRNRAIAHLMLNFGMIDK
HHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHCCCCC
NIDEALDLYFQQCSVLVNCHDLAVMAATLAHKGINPITQERAVESGYVKDILSVMYTCGM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
YDFAGEWAYKVGLPAKSGVSGGIMAVVPNRAGIAVFSPLLDEYGNSIRGRRVLEALSQKL
HHHCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GLHLFDLSMGGCGLQAAMDSN
CCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA