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The map label for this gene is cysH [H]

Identifier: 158333551

GI number: 158333551

Start: 320754

End: 321482

Strand: Reverse

Name: cysH [H]

Synonym: AM1_0350

Alternate gene names: 158333551

Gene position: 321482-320754 (Counterclockwise)

Preceding gene: 158333553

Following gene: 158333550

Centisome position: 4.94

GC content: 49.52

Gene sequence:

>729_bases
ATGGATCCATGCATCGAGACCCCCAAGCAGCAGACAGTTGATATTGATCAGCTGAATCAGCAGTTGGATAAGGCTCATCC
CAAAAGTATTTTGACGTGGTGTTTAGAGAATCTACCTGAGGGGCTGATCCAGAGCACCGCATTTGGGGCGAGTGGCATGG
TGATTATGGACCTGCTTTACAAAGACCTCAATCCCAATCCTTCCATTCCGGTCTTGTTTTTGGATACCCTGCACCATTTT
CCAGAAACCCTAAAACTGGCCAACGACGCTCAGGCCCATTACGGGGTAGATTTGCAAATTTTTCGCCCCCAAGGTGTGAC
GTCCCGTGCTGAATTTGCTCAACACTATGGTGAGCAGCTCTGGGAAACGGATTTAGAACAATTTCATCAACTAACCAAAG
TTGAACCGTTGCGGCGAGGACTCGAACAGCTTCAGGTCGCCACCTGGATTACGGGCAGGCGACGAGATCAGTCTTCTACC
CGAGCAAAGATGCCGATTTTTGAATGCGATCGAGACGGGCGCTTAAAAGTGAATCCCCTAGCCAACTGGTCTTACAAAGA
TGTGTGGAACTATCTGGTCAACAATCAGGTGTTGTATAACCCCCTTTATGATCAGGGTTATGCCAGTATCGGCGATGAGC
CTTTAACCACCATGACGAAGCCTGGTGAAGAAGAGCGGGCCGGTCGTTGGCGAGGAAAAGCCCGTACAGAATGCGGAATG
CATGCTTAA

Upstream 100 bases:

>100_bases
CAACGGGACCGAACTTTATACTACGGTTATCCGGTGGAGATTCGGGGAATAAGCAGTTAGAATGTTCGTACCGAATTTGA
AATAGGTTGGCATTGGCACT

Downstream 100 bases:

>100_bases
GAATGTCTGCTCCCAACATTAAAGTAACCGTCAAGCTGTTTGCCGCTTATCAGGAGGTAGTGGGTTCCCCAGAGTTAGAA
CTCGAATTTCCAGATGGCTC

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDPCIETPKQQTVDIDQLNQQLDKAHPKSILTWCLENLPEGLIQSTAFGASGMVIMDLLYKDLNPNPSIPVLFLDTLHHF
PETLKLANDAQAHYGVDLQIFRPQGVTSRAEFAQHYGEQLWETDLEQFHQLTKVEPLRRGLEQLQVATWITGRRRDQSST
RAKMPIFECDRDGRLKVNPLANWSYKDVWNYLVNNQVLYNPLYDQGYASIGDEPLTTMTKPGEEERAGRWRGKARTECGM
HA

Sequences:

>Translated_242_residues
MDPCIETPKQQTVDIDQLNQQLDKAHPKSILTWCLENLPEGLIQSTAFGASGMVIMDLLYKDLNPNPSIPVLFLDTLHHF
PETLKLANDAQAHYGVDLQIFRPQGVTSRAEFAQHYGEQLWETDLEQFHQLTKVEPLRRGLEQLQVATWITGRRRDQSST
RAKMPIFECDRDGRLKVNPLANWSYKDVWNYLVNNQVLYNPLYDQGYASIGDEPLTTMTKPGEEERAGRWRGKARTECGM
HA
>Mature_242_residues
MDPCIETPKQQTVDIDQLNQQLDKAHPKSILTWCLENLPEGLIQSTAFGASGMVIMDLLYKDLNPNPSIPVLFLDTLHHF
PETLKLANDAQAHYGVDLQIFRPQGVTSRAEFAQHYGEQLWETDLEQFHQLTKVEPLRRGLEQLQVATWITGRRRDQSST
RAKMPIFECDRDGRLKVNPLANWSYKDVWNYLVNNQVLYNPLYDQGYASIGDEPLTTMTKPGEEERAGRWRGKARTECGM
HA

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:
GO:0003824: Phosphoadenosine phosphosulfate reductase
GO:0004604: Phosphoadenosine phosphosulfate reductase
GO:0008152: Phosphoadenosine phosphosulfate reductase
GO:0016491: Phosphoadenosine phosphosulfate reductase
GO:0019344: Phosphoadenosine phosphosulfate reductase
GO:0019379: Phosphoadenosine phosphosulfate reductase
GO:0055114: Phosphoadenosine phosphosulfate reductase

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=245, Percent_Identity=32.2448979591837, Blast_Score=141, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325425, Length=234, Percent_Identity=44.8717948717949, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24584122, Length=181, Percent_Identity=28.7292817679558, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 27754; Mature: 27754

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPCIETPKQQTVDIDQLNQQLDKAHPKSILTWCLENLPEGLIQSTAFGASGMVIMDLLY
CCCCCCCCCHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KDLNPNPSIPVLFLDTLHHFPETLKLANDAQAHYGVDLQIFRPQGVTSRAEFAQHYGEQL
HHCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
WETDLEQFHQLTKVEPLRRGLEQLQVATWITGRRRDQSSTRAKMPIFECDRDGRLKVNPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEECCCCCCEEECCC
ANWSYKDVWNYLVNNQVLYNPLYDQGYASIGDEPLTTMTKPGEEERAGRWRGKARTECGM
CCCCHHHHHHHHHCCCEEECCCHHCCCHHCCCCCCHHCCCCCCHHHHHHCCCCCCCCCCC
HA
CC
>Mature Secondary Structure
MDPCIETPKQQTVDIDQLNQQLDKAHPKSILTWCLENLPEGLIQSTAFGASGMVIMDLLY
CCCCCCCCCHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KDLNPNPSIPVLFLDTLHHFPETLKLANDAQAHYGVDLQIFRPQGVTSRAEFAQHYGEQL
HHCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
WETDLEQFHQLTKVEPLRRGLEQLQVATWITGRRRDQSSTRAKMPIFECDRDGRLKVNPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEECCCCCCEEECCC
ANWSYKDVWNYLVNNQVLYNPLYDQGYASIGDEPLTTMTKPGEEERAGRWRGKARTECGM
CCCCHHHHHHHHHCCCEEECCCHHCCCHHCCCCCCHHCCCCCCHHHHHHCCCCCCCCCCC
HA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]