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The map label for this gene is pheT [C]

Identifier: 158333522

GI number: 158333522

Start: 294350

End: 295651

Strand: Direct

Name: pheT [C]

Synonym: AM1_0314

Alternate gene names: 158333522

Gene position: 294350-295651 (Clockwise)

Preceding gene: 158333521

Following gene: 158333523

Centisome position: 4.53

GC content: 49.08

Gene sequence:

>1302_bases
ATGACTGTCATTGTGATGGAAGTGATTAAGGTTGCGCGGCATCCCAATGCTGACTCGCTTAATGTGTACGAGATGCAAGT
ACCTGGCCACCCTTCTGTGCAGGTGATAGGAAATTTGGAGCGGATTTATGGGGTTGGTGAACATGTCCTGGTTGCCCAGG
TCAATTCTGTTCTCAAAGACGGCACCAAAATCAAAGCAACCAAATTGCGTGGCATCATCTCCTACGGGATGGCACTGGAG
AAAACGGAATTACCGGTTGGTGAAGATGTATCTCAAACCTATTGCCAGCAGTCCGTTGTGCAGTCGGTACAAATGCAGCG
ATGGCCCAGTGTGGAACTCCTGCATAATGTTCGTCGGAGTTTAATGGCCCTGGAAGCCACTCCAATGGTAACCTATCGAG
CAAAAATCAAACTGGATGGAACCAATGCCGGGGTGCAAATCTTCTCAGATGGCCGCGTGGCAGCCCAAAGCCGTTCCCAA
ATCATCACACCTGATGATGACAATATGGGATTTGCCCAATGGGTCAGTCAACAGATGGATTACTTTGCTCCGTTGGCGGG
TCACGAACACATCACAATCTTTGGAGAATGGTGTGGTCGGGGGATTCAGAAGCGGACTTCTATTTCCAAAATCGATCGCA
GAATCTTTACGGTGTTTGCGGTCCAGTATGGCGGAGTAGAAAATGAAGTCGCCAAGCTGGAAATCGATCCAGATAAGATA
TCCCAGCTACTCCCATCTCACCCGGACATTTTTGTCCTGCCGTTTATGGGAGAGCCATTGACCTTGGATTTTGGCAACCG
AGACCAATTGGAATCCGTCGCCAATGCCATCAACCAGCTCGTCTCCGAAGTTGAGCAAATTGACCCTTGGGTTAAAGCCA
CTTTCGGGATTGAAGGGTTGGGAGAAGGGTTGGTTCTCTATCCCCAAACCCAAACTCGAGTTGATCGACTGACATACCCT
GAGCTGATGTTTAAGGCGAAAGGCGAAAAGCATCAGGTAGTCAAAACCAAACAGCCCGTGCAGCTAGACCCTGAATTCGT
TAAGAGTATTGATGAATTTGTAGCGTTGATGGTAACTCCTGCTCGACTGGAGCAAGGGTTATCTGAGAGCTGCCAGGGGC
CATTGGAGATGAAGCAGTTGGGGGGCTTCCTGAAGTGGCTAGCTCAGGATGTGCAAAAAGAAAGCGAAGCTGAATTAGAA
GCAGCCAAACTTTCTTGGAAGGATGTCAATAAAGCGGTTACCCATTCTGGGAAGTCTTGGTTTCGCGATCGCATCCTCTC
CCTCACTGACATTGCGCAGTAA

Upstream 100 bases:

>100_bases
TTAGGATAGTTCAAATCTACGTATTTTATATTCCTCACCGGAGTGAATATATCGTTACTTCCAAGTTTTCGTTCATATTG
CAAGTCAAAGGAGGTGTGAA

Downstream 100 bases:

>100_bases
ATGGGTGGTCACTGTTCGCTTCTGTGGGAGTTTAACAGTGGCCACCGCCCAACCTCGATTGCCTGGTGAACACCAGCTCA
GAATTGCTTGCGAGTCAAAA

Product: phenylalanyl-tRNA synthetase beta subunit

Products: AMP; diphosphate; L-phenylalanyl-tRNAPhe [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTVIVMEVIKVARHPNADSLNVYEMQVPGHPSVQVIGNLERIYGVGEHVLVAQVNSVLKDGTKIKATKLRGIISYGMALE
KTELPVGEDVSQTYCQQSVVQSVQMQRWPSVELLHNVRRSLMALEATPMVTYRAKIKLDGTNAGVQIFSDGRVAAQSRSQ
IITPDDDNMGFAQWVSQQMDYFAPLAGHEHITIFGEWCGRGIQKRTSISKIDRRIFTVFAVQYGGVENEVAKLEIDPDKI
SQLLPSHPDIFVLPFMGEPLTLDFGNRDQLESVANAINQLVSEVEQIDPWVKATFGIEGLGEGLVLYPQTQTRVDRLTYP
ELMFKAKGEKHQVVKTKQPVQLDPEFVKSIDEFVALMVTPARLEQGLSESCQGPLEMKQLGGFLKWLAQDVQKESEAELE
AAKLSWKDVNKAVTHSGKSWFRDRILSLTDIAQ

Sequences:

>Translated_433_residues
MTVIVMEVIKVARHPNADSLNVYEMQVPGHPSVQVIGNLERIYGVGEHVLVAQVNSVLKDGTKIKATKLRGIISYGMALE
KTELPVGEDVSQTYCQQSVVQSVQMQRWPSVELLHNVRRSLMALEATPMVTYRAKIKLDGTNAGVQIFSDGRVAAQSRSQ
IITPDDDNMGFAQWVSQQMDYFAPLAGHEHITIFGEWCGRGIQKRTSISKIDRRIFTVFAVQYGGVENEVAKLEIDPDKI
SQLLPSHPDIFVLPFMGEPLTLDFGNRDQLESVANAINQLVSEVEQIDPWVKATFGIEGLGEGLVLYPQTQTRVDRLTYP
ELMFKAKGEKHQVVKTKQPVQLDPEFVKSIDEFVALMVTPARLEQGLSESCQGPLEMKQLGGFLKWLAQDVQKESEAELE
AAKLSWKDVNKAVTHSGKSWFRDRILSLTDIAQ
>Mature_432_residues
TVIVMEVIKVARHPNADSLNVYEMQVPGHPSVQVIGNLERIYGVGEHVLVAQVNSVLKDGTKIKATKLRGIISYGMALEK
TELPVGEDVSQTYCQQSVVQSVQMQRWPSVELLHNVRRSLMALEATPMVTYRAKIKLDGTNAGVQIFSDGRVAAQSRSQI
ITPDDDNMGFAQWVSQQMDYFAPLAGHEHITIFGEWCGRGIQKRTSISKIDRRIFTVFAVQYGGVENEVAKLEIDPDKIS
QLLPSHPDIFVLPFMGEPLTLDFGNRDQLESVANAINQLVSEVEQIDPWVKATFGIEGLGEGLVLYPQTQTRVDRLTYPE
LMFKAKGEKHQVVKTKQPVQLDPEFVKSIDEFVALMVTPARLEQGLSESCQGPLEMKQLGGFLKWLAQDVQKESEAELEA
AKLSWKDVNKAVTHSGKSWFRDRILSLTDIAQ

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:
GO:0000049: Phenylalanyl-tRNA synthetase beta subunit, putative
GO:0004812: Phenylalanyl-tRNA synthetase beta subunit, putative

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.1.1.20 [C]

Molecular weight: Translated: 48389; Mature: 48258

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIVMEVIKVARHPNADSLNVYEMQVPGHPSVQVIGNLERIYGVGEHVLVAQVNSVLKD
CEEEHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHCCHHHHHCCCCHHHHHHHHHHHHC
GTKIKATKLRGIISYGMALEKTELPVGEDVSQTYCQQSVVQSVQMQRWPSVELLHNVRRS
CCEEEHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LMALEATPMVTYRAKIKLDGTNAGVQIFSDGRVAAQSRSQIITPDDDNMGFAQWVSQQMD
HHHHCCCCEEEEEEEEEECCCCCCEEEEECCCEEHHCCCCEECCCCCCCCHHHHHHHHHH
YFAPLAGHEHITIFGEWCGRGIQKRTSISKIDRRIFTVFAVQYGGVENEVAKLEIDPDKI
HHCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHH
SQLLPSHPDIFVLPFMGEPLTLDFGNRDQLESVANAINQLVSEVEQIDPWVKATFGIEGL
HHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
GEGLVLYPQTQTRVDRLTYPELMFKAKGEKHQVVKTKQPVQLDPEFVKSIDEFVALMVTP
CCCEEEECCHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
ARLEQGLSESCQGPLEMKQLGGFLKWLAQDVQKESEAELEAAKLSWKDVNKAVTHSGKSW
HHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
FRDRILSLTDIAQ
HHHHHHHHHHCCC
>Mature Secondary Structure 
TVIVMEVIKVARHPNADSLNVYEMQVPGHPSVQVIGNLERIYGVGEHVLVAQVNSVLKD
EEEHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHCCHHHHHCCCCHHHHHHHHHHHHC
GTKIKATKLRGIISYGMALEKTELPVGEDVSQTYCQQSVVQSVQMQRWPSVELLHNVRRS
CCEEEHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LMALEATPMVTYRAKIKLDGTNAGVQIFSDGRVAAQSRSQIITPDDDNMGFAQWVSQQMD
HHHHCCCCEEEEEEEEEECCCCCCEEEEECCCEEHHCCCCEECCCCCCCCHHHHHHHHHH
YFAPLAGHEHITIFGEWCGRGIQKRTSISKIDRRIFTVFAVQYGGVENEVAKLEIDPDKI
HHCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHH
SQLLPSHPDIFVLPFMGEPLTLDFGNRDQLESVANAINQLVSEVEQIDPWVKATFGIEGL
HHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
GEGLVLYPQTQTRVDRLTYPELMFKAKGEKHQVVKTKQPVQLDPEFVKSIDEFVALMVTP
CCCEEEECCHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
ARLEQGLSESCQGPLEMKQLGGFLKWLAQDVQKESEAELEAAKLSWKDVNKAVTHSGKSW
HHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
FRDRILSLTDIAQ
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} 0.009 {ATP}} [C]

Substrates: ATP; @PHEN01.txt*phenylalanine!; tRNAPhe [C]

Specific reaction: ATP + @PHEN01.txt*phenylalanine! + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA