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The map label for this gene is barA [H]

Identifier: 158333448

GI number: 158333448

Start: 215132

End: 217399

Strand: Direct

Name: barA [H]

Synonym: AM1_0220

Alternate gene names: 158333448

Gene position: 215132-217399 (Clockwise)

Preceding gene: 158333447

Following gene: 158333455

Centisome position: 3.31

GC content: 48.85

Gene sequence:

>2268_bases
GTGTTTAAGCTACTGCGATACTATTCCATCACCAGCTTTGTGGCTTTCGTAGTGGCCATTGCTGCCCTGAATCAGTACAC
GCGAGATCGTGCCATTCAGAGCTTAGTCACAGTTACTGAGAATAAGAATGTTGCCCTGACTCAATCCTTTGCTAATACCC
TCTGGAAGACCCATGGATCCTTCTTAAGCAATACTCAATCTTTGACAGCAAAACAGTTAAAGTCCCATCCCCAAACCCTG
AAGCTGACGAAGGATATCGAATCCTGGGCAGAAGGACTCCCCGTGTTGAAAGTAAAGGTCTTTGATACACAAGGGCGGAC
TGTCTTTTCAACGAGTACCAAGCAAATTGGCCAAGACAAGCGTCAATCAAAAGGCTTCCAGGCAGCCATTCAGGGGCAAT
CCCGCTCCTTCATCAACCATAAAGACACCTTTACAGGTATGAAGGGCCAGATGAAGGATCGCAATCTTCTGTCGAGCTAT
CTACCTCTGCGTCCCGATGGCCCAGACGGCTCCATTGAAGGGGTATTTGAGATGTATACCGATGTCACCCCGTTGCTGGA
ACGAGTGGAAACCACCCAGCGCAATATTTTGTGGGGAACCAGTGGGGTGCTTGGGTTGTTGTATGGAGTTCTGTTTGCAA
TTGTCTATCGAGGTGATCGCATTATTCAAGAAAAGCATGCTGCCGCTGCTGCCGCCGCCCGAGCAAAAGACGACTTCTTG
GCGATGATGAGTCACGAAATTCGCACACCAATGAATGGCGTGATTGGCATGACTGGCTTGCTCCTGGATACAGAGTTAAC
GCCTCAACAGCAAGAATATACAGAAACGATTCGTAAAAGTGGGGATAGTCTCCTCACGCTTATTAATGACATTCTGGATT
ACTCCAAAATTGAAGCCGGTAAGCTGGATCTGGAAAAACAGGCATTTAATGTCCGGGTTTGTGTGGAAGATGCCTTAGAG
CTGGTGGCCTCTCGGGTGGGTCCCAAGGAGATTGAGTTGGCAGGGGTGGTTGAACCCGATGTTCCCCAGGCGATTTTGGG
AGATGTGACCCGCCTACGCCAGATTCTCGTCAACCTGTTGGGCAATGCCATCAAGTTCACCCAATCTGGGGAAGTCGTGG
TTAACGTTTCGGCTGAGCCCTTTGCAGCGGATACTCCTTTGTATGAACTCAGTTTTGCCGTCAGAGATACGGGTATGGGG
ATTCCTGCTGAACGCCAGAATCGTCTATTCAAATCCTTTAGCCAGGTGGACTCATCGACCACCCGTAAATTTGGTGGAAC
AGGGTTGGGGCTAGTCATCTGTAAGCGTTTAAGTGAACTGATGGGCGGTCGGATTTGGGTTGAAAGTGAAGAAGGTCAAG
GGTCCACCTTTGCCTTTACGATTCAAGCCGAAGAGACGGAAGATACAACGGTGTTGGCTCAATCGCTACCTGCTGTTACC
TTGAAGGACAAGCGGGTGCTGCTTGTGGATGATAACGACACCAATCAACGGGTTCTCACCCTGCAAACAGAATCTTGGAA
AATGAAACCTCAGGTGGCTGCGTCTGGCTACGAGGCTTTAGGGCTGTTGGAACATGCGGGTCCTTTTGATATCGCGATTC
TGGATATGCAAATGCCTGAAATGGATGGCCTCACTTTGGCTCAGAAAATTCGCAGTCATCCTCGGGGGGTAAACTTCCCA
ATGGTAATGTTGACCTCCATTGGTCAGGCTCCTGACTTAGCCCATCACAAAGGTCTGTTTTCAGCCTGGCTCAATAAGCC
GATCAAACAATCCCAGCTCTATAGCGCCCTGGTCAAATCCTTAACGGGAAATGAAGTCAAAACTCAAAAGTCTACTACTC
TTCAGCTAGATACAGAGATGGCGGATCAGCATCCCTTGAAGATTTTGCTGGCGGAAGATAACCCCGTCAACCAGAAACTG
GCTGTGTTGATTTTAGCCAAAATGGGCTATCGCCCTGATGTTGTGGGCAATGGTTTGGAAGTTCTGCAAGCCCTCAAGCG
CCAATCCTATGATGTCATTCTGATGGATGTTAATATGCCGGAAATGGACGGGCTGACGGCAACAACCCATATTTACCAAT
CGATAATGCCGGAAGAACGACCCAGGATTATTGCGATGACCGCCAATGCCATGCAGGGGGATCGCGAAAAATGCCTGGAT
GTTGGCATGGATGACTACGTGACCAAACCCATCAATATTCAGGAACTTGTGGTTGCCTTGGAGAATAGCGAGCCTCAAAC
AACAGAAAGTCAGCCGATTAAAACATGA

Upstream 100 bases:

>100_bases
TTCATATAAATGGGTACTCTACGGACATCAGTTAATGGGTGAGGTGGGGGAGTATCTCTCCTTATCACTAGCGGTCCAAT
TTACTCCGTGAGCGTTTCAA

Downstream 100 bases:

>100_bases
GGTTGAATCATTTAGTTAGAGTAATGAGAGCTTGGGTTTTAATCAAAATATCCTTTGGATTCTACTGAACTGGGATAAAA
TCTCTCAATAATTTCAAGAA

Product: two-component hybrid sensor and regulator histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MFKLLRYYSITSFVAFVVAIAALNQYTRDRAIQSLVTVTENKNVALTQSFANTLWKTHGSFLSNTQSLTAKQLKSHPQTL
KLTKDIESWAEGLPVLKVKVFDTQGRTVFSTSTKQIGQDKRQSKGFQAAIQGQSRSFINHKDTFTGMKGQMKDRNLLSSY
LPLRPDGPDGSIEGVFEMYTDVTPLLERVETTQRNILWGTSGVLGLLYGVLFAIVYRGDRIIQEKHAAAAAAARAKDDFL
AMMSHEIRTPMNGVIGMTGLLLDTELTPQQQEYTETIRKSGDSLLTLINDILDYSKIEAGKLDLEKQAFNVRVCVEDALE
LVASRVGPKEIELAGVVEPDVPQAILGDVTRLRQILVNLLGNAIKFTQSGEVVVNVSAEPFAADTPLYELSFAVRDTGMG
IPAERQNRLFKSFSQVDSSTTRKFGGTGLGLVICKRLSELMGGRIWVESEEGQGSTFAFTIQAEETEDTTVLAQSLPAVT
LKDKRVLLVDDNDTNQRVLTLQTESWKMKPQVAASGYEALGLLEHAGPFDIAILDMQMPEMDGLTLAQKIRSHPRGVNFP
MVMLTSIGQAPDLAHHKGLFSAWLNKPIKQSQLYSALVKSLTGNEVKTQKSTTLQLDTEMADQHPLKILLAEDNPVNQKL
AVLILAKMGYRPDVVGNGLEVLQALKRQSYDVILMDVNMPEMDGLTATTHIYQSIMPEERPRIIAMTANAMQGDREKCLD
VGMDDYVTKPINIQELVVALENSEPQTTESQPIKT

Sequences:

>Translated_755_residues
MFKLLRYYSITSFVAFVVAIAALNQYTRDRAIQSLVTVTENKNVALTQSFANTLWKTHGSFLSNTQSLTAKQLKSHPQTL
KLTKDIESWAEGLPVLKVKVFDTQGRTVFSTSTKQIGQDKRQSKGFQAAIQGQSRSFINHKDTFTGMKGQMKDRNLLSSY
LPLRPDGPDGSIEGVFEMYTDVTPLLERVETTQRNILWGTSGVLGLLYGVLFAIVYRGDRIIQEKHAAAAAAARAKDDFL
AMMSHEIRTPMNGVIGMTGLLLDTELTPQQQEYTETIRKSGDSLLTLINDILDYSKIEAGKLDLEKQAFNVRVCVEDALE
LVASRVGPKEIELAGVVEPDVPQAILGDVTRLRQILVNLLGNAIKFTQSGEVVVNVSAEPFAADTPLYELSFAVRDTGMG
IPAERQNRLFKSFSQVDSSTTRKFGGTGLGLVICKRLSELMGGRIWVESEEGQGSTFAFTIQAEETEDTTVLAQSLPAVT
LKDKRVLLVDDNDTNQRVLTLQTESWKMKPQVAASGYEALGLLEHAGPFDIAILDMQMPEMDGLTLAQKIRSHPRGVNFP
MVMLTSIGQAPDLAHHKGLFSAWLNKPIKQSQLYSALVKSLTGNEVKTQKSTTLQLDTEMADQHPLKILLAEDNPVNQKL
AVLILAKMGYRPDVVGNGLEVLQALKRQSYDVILMDVNMPEMDGLTATTHIYQSIMPEERPRIIAMTANAMQGDREKCLD
VGMDDYVTKPINIQELVVALENSEPQTTESQPIKT
>Mature_755_residues
MFKLLRYYSITSFVAFVVAIAALNQYTRDRAIQSLVTVTENKNVALTQSFANTLWKTHGSFLSNTQSLTAKQLKSHPQTL
KLTKDIESWAEGLPVLKVKVFDTQGRTVFSTSTKQIGQDKRQSKGFQAAIQGQSRSFINHKDTFTGMKGQMKDRNLLSSY
LPLRPDGPDGSIEGVFEMYTDVTPLLERVETTQRNILWGTSGVLGLLYGVLFAIVYRGDRIIQEKHAAAAAAARAKDDFL
AMMSHEIRTPMNGVIGMTGLLLDTELTPQQQEYTETIRKSGDSLLTLINDILDYSKIEAGKLDLEKQAFNVRVCVEDALE
LVASRVGPKEIELAGVVEPDVPQAILGDVTRLRQILVNLLGNAIKFTQSGEVVVNVSAEPFAADTPLYELSFAVRDTGMG
IPAERQNRLFKSFSQVDSSTTRKFGGTGLGLVICKRLSELMGGRIWVESEEGQGSTFAFTIQAEETEDTTVLAQSLPAVT
LKDKRVLLVDDNDTNQRVLTLQTESWKMKPQVAASGYEALGLLEHAGPFDIAILDMQMPEMDGLTLAQKIRSHPRGVNFP
MVMLTSIGQAPDLAHHKGLFSAWLNKPIKQSQLYSALVKSLTGNEVKTQKSTTLQLDTEMADQHPLKILLAEDNPVNQKL
AVLILAKMGYRPDVVGNGLEVLQALKRQSYDVILMDVNMPEMDGLTATTHIYQSIMPEERPRIIAMTANAMQGDREKCLD
VGMDDYVTKPINIQELVVALENSEPQTTESQPIKT

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=509, Percent_Identity=36.7387033398821, Blast_Score=292, Evalue=5e-80,
Organism=Escherichia coli, GI145693157, Length=515, Percent_Identity=33.3980582524272, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI48994928, Length=348, Percent_Identity=38.2183908045977, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081816, Length=339, Percent_Identity=35.9882005899705, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788713, Length=349, Percent_Identity=34.9570200573066, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=30.1310043668122, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=30.4932735426009, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=25.609756097561, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786783, Length=231, Percent_Identity=28.1385281385281, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788393, Length=212, Percent_Identity=28.7735849056604, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=25.531914893617, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1787229, Length=118, Percent_Identity=34.7457627118644, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790300, Length=241, Percent_Identity=27.3858921161826, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87082012, Length=112, Percent_Identity=33.9285714285714, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1790861, Length=399, Percent_Identity=21.5538847117794, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=32.3076923076923, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1787894, Length=227, Percent_Identity=23.3480176211454, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=367, Percent_Identity=28.6103542234332, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322000, Length=111, Percent_Identity=40.5405405405405, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 83462; Mature: 83462

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLLRYYSITSFVAFVVAIAALNQYTRDRAIQSLVTVTENKNVALTQSFANTLWKTHGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCH
FLSNTQSLTAKQLKSHPQTLKLTKDIESWAEGLPVLKVKVFDTQGRTVFSTSTKQIGQDK
HHHCHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEEEEECCCCEEEECCHHHHCCHH
RQSKGFQAAIQGQSRSFINHKDTFTGMKGQMKDRNLLSSYLPLRPDGPDGSIEGVFEMYT
HHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
DVTPLLERVETTQRNILWGTSGVLGLLYGVLFAIVYRGDRIIQEKHAAAAAAARAKDDFL
HHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AMMSHEIRTPMNGVIGMTGLLLDTELTPQQQEYTETIRKSGDSLLTLINDILDYSKIEAG
HHHHHHHCCCCCHHHHHHHEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
KLDLEKQAFNVRVCVEDALELVASRVGPKEIELAGVVEPDVPQAILGDVTRLRQILVNLL
CCCCCHHHEEEEEEHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
GNAIKFTQSGEVVVNVSAEPFAADTPLYELSFAVRDTGMGIPAERQNRLFKSFSQVDSST
CCCEEEECCCCEEEEECCCCCCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHCCHH
TRKFGGTGLGLVICKRLSELMGGRIWVESEEGQGSTFAFTIQAEETEDTTVLAQSLPAVT
HHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHCCCEE
LKDKRVLLVDDNDTNQRVLTLQTESWKMKPQVAASGYEALGLLEHAGPFDIAILDMQMPE
ECCCEEEEEECCCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHCCCCCEEEEEEECCCC
MDGLTLAQKIRSHPRGVNFPMVMLTSIGQAPDLAHHKGLFSAWLNKPIKQSQLYSALVKS
CCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LTGNEVKTQKSTTLQLDTEMADQHPLKILLAEDNPVNQKLAVLILAKMGYRPDVVGNGLE
HCCCCCCCCCCCEEEEEHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHH
VLQALKRQSYDVILMDVNMPEMDGLTATTHIYQSIMPEERPRIIAMTANAMQGDREKCLD
HHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHCCCHHHHHH
VGMDDYVTKPINIQELVVALENSEPQTTESQPIKT
CCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKLLRYYSITSFVAFVVAIAALNQYTRDRAIQSLVTVTENKNVALTQSFANTLWKTHGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCH
FLSNTQSLTAKQLKSHPQTLKLTKDIESWAEGLPVLKVKVFDTQGRTVFSTSTKQIGQDK
HHHCHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEEEEECCCCEEEECCHHHHCCHH
RQSKGFQAAIQGQSRSFINHKDTFTGMKGQMKDRNLLSSYLPLRPDGPDGSIEGVFEMYT
HHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
DVTPLLERVETTQRNILWGTSGVLGLLYGVLFAIVYRGDRIIQEKHAAAAAAARAKDDFL
HHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AMMSHEIRTPMNGVIGMTGLLLDTELTPQQQEYTETIRKSGDSLLTLINDILDYSKIEAG
HHHHHHHCCCCCHHHHHHHEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
KLDLEKQAFNVRVCVEDALELVASRVGPKEIELAGVVEPDVPQAILGDVTRLRQILVNLL
CCCCCHHHEEEEEEHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
GNAIKFTQSGEVVVNVSAEPFAADTPLYELSFAVRDTGMGIPAERQNRLFKSFSQVDSST
CCCEEEECCCCEEEEECCCCCCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHCCHH
TRKFGGTGLGLVICKRLSELMGGRIWVESEEGQGSTFAFTIQAEETEDTTVLAQSLPAVT
HHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHCCCEE
LKDKRVLLVDDNDTNQRVLTLQTESWKMKPQVAASGYEALGLLEHAGPFDIAILDMQMPE
ECCCEEEEEECCCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHCCCCCEEEEEEECCCC
MDGLTLAQKIRSHPRGVNFPMVMLTSIGQAPDLAHHKGLFSAWLNKPIKQSQLYSALVKS
CCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LTGNEVKTQKSTTLQLDTEMADQHPLKILLAEDNPVNQKLAVLILAKMGYRPDVVGNGLE
HCCCCCCCCCCCEEEEEHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHH
VLQALKRQSYDVILMDVNMPEMDGLTATTHIYQSIMPEERPRIIAMTANAMQGDREKCLD
HHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHCCCHHHHHH
VGMDDYVTKPINIQELVVALENSEPQTTESQPIKT
CCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]