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The map label for this gene is kefA [H]

Identifier: 158333303

GI number: 158333303

Start: 73899

End: 75623

Strand: Reverse

Name: kefA [H]

Synonym: AM1_0073

Alternate gene names: 158333303

Gene position: 75623-73899 (Counterclockwise)

Preceding gene: 158333304

Following gene: 158333301

Centisome position: 1.16

GC content: 46.2

Gene sequence:

>1725_bases
ATGATCATCAACCGATTATTCGCCTGGGGAGTTTTGGCCATTAGGGATTTTTCTGATCGGCGCTGGTGGCGTTGGTGCTG
TCTTGCCCTGATTACGGCAAGCTTAATTCTCTTAAGTCATCCCAGTAGGGATGTCGTTGTGGCTCAAACCCTTGAGTCAG
AGACGGTGGAAGTGGATGGCATTCCGTTATTTGAATTAAGTTCGTCGAAAGAATTCCCGGCTGAAGATAGAGCTAGAACG
GTCAACCAAAGCTTACAAACCCTGGTGAATACCTCGACAAAGGCAGAGGTTATTGTTGATTTCTCTAAAGACCTACCCGT
GCTGAAAGTCGAAGTCGGTGCGAAACAACCCTTGTATTTGTTGACCGTGAATAGTCAGGATGCAGAGTTGTCTTCTCCAG
AAGTGACCGCTAGAACTTGGCAAAAGAAATTGCAGCGAGCAATTAATCGAGGGCAAGATGAACGGGCCAATCCTGGGCGA
TTAGTGGCTATTGGCGCTCTATGTTTGTTGTTGGCACTGATCGCTCACTGGGGGATAGGGTGGTTTTGGCGCCAGAAGTT
ATTCAAACTTTTACCTCGGGCAACGGTCGATCCAACGACGGGTGAACAGCCTCAAAGCCTTAGATTTCTCCTCAAAGCCA
TTTTCTACTGTTTGCGGATCGTGCTGTGGGTGGGCACGATTGGCTTTGTTTGTGGTCTATTTCCCTGGACCCGCAATGGC
ATCTATTTAATCATCAACACTCTGATTCAAACCTTCTCCACTCCGTTTATTCCGGCAGGTGGAAAGTCCTACTCTGTTGT
AGATGTTGCTCTGCTTCTACTTCTACTGGCGGGGCTAATGAACCTGACTAAAACCGTGCAGCATTTGTTGAGATCACGGG
TATTAGGAGCCACTGGACTGAATCGCGGAGCCCAAGAAGCCGTGGCCTTTGTGGCTAACTATGTCTTGCTGTTCATCGGA
GCAATGGTGCTATTTCAGCTTTGGGGCTTTGATCTGAGTTCCTTAGCTGTATTTGCGAGTGTCCTTGGTGTGGGGATTGG
TTTAGGACTGCAAGGATTTGCCAAAAACTTTATTAGTGGTATGGTCCTGATTTTTGAGCAACCCATTAAGGTGGGGGACT
TTGTGAAGGTCGGAGATTATCAGGGCACAGTAGAACGAATCAATGTTCGGAGTACAGAGATTCGCACCCTAGATCAAGTT
TCGATTATTGTCCCTAATTCAGAGTTTTTAGAATCTGAAGTGGTTAATTGGGACCATGGTTCTTCTGTCTCGCGGTTACA
AATTCCAGTGGGAGTGGCCTATGGGACCAACCCCAAAAAGGTACGAGAAGCTTTGATGGATGCGGTTGCTGAGTATAGTT
CGGTTCGCAAAGATCCACCCCCTCGGGTATTTTTCACTGAGTTTGGGGATAATTCTCTGAATTTCTTGCTATTGGTTTGG
ATTGATGAGCCTGAAAAGCAATTTGCCATCAAGAGCGATCTTAATTTTCGCATTGAGACCATTCTGCGTCATCGAGAGAT
TGAGATTCCTTTTCCTCAAAGGGATCTACATGTGCGATCCGGCAAGCTACCCCTAGATCTACCTGAAGAGTTAGTGACTT
CTTTTACGCTACTTTCTCAAAATTTAAACGTTTGGTTGCAGCAGCAGTCTCTAAAAGAAAACGCTTCAGACACTCATGCT
GCTGCAAATACAAAGTCTGCTCACCCAGATGATAAAAAGATTTAG

Upstream 100 bases:

>100_bases
GTCCTCCATCAGTTCTGAAGGCGAAGCCGTCGGTGTGTTGGAATATCGCATTCAATTCTAATCCTCGTTACAATAACCCT
TTGCAATAGATACGGCTGCT

Downstream 100 bases:

>100_bases
TTCCCTGTTTGTCCTTCAAAAGGATACTCTTAGGTGCATCAGTTTAACTAATCTTGAATAAGGGGAATTTACCTAGATAA
CTTAGGTCTAGTCGCCACCA

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MIINRLFAWGVLAIRDFSDRRWWRWCCLALITASLILLSHPSRDVVVAQTLESETVEVDGIPLFELSSSKEFPAEDRART
VNQSLQTLVNTSTKAEVIVDFSKDLPVLKVEVGAKQPLYLLTVNSQDAELSSPEVTARTWQKKLQRAINRGQDERANPGR
LVAIGALCLLLALIAHWGIGWFWRQKLFKLLPRATVDPTTGEQPQSLRFLLKAIFYCLRIVLWVGTIGFVCGLFPWTRNG
IYLIINTLIQTFSTPFIPAGGKSYSVVDVALLLLLLAGLMNLTKTVQHLLRSRVLGATGLNRGAQEAVAFVANYVLLFIG
AMVLFQLWGFDLSSLAVFASVLGVGIGLGLQGFAKNFISGMVLIFEQPIKVGDFVKVGDYQGTVERINVRSTEIRTLDQV
SIIVPNSEFLESEVVNWDHGSSVSRLQIPVGVAYGTNPKKVREALMDAVAEYSSVRKDPPPRVFFTEFGDNSLNFLLLVW
IDEPEKQFAIKSDLNFRIETILRHREIEIPFPQRDLHVRSGKLPLDLPEELVTSFTLLSQNLNVWLQQQSLKENASDTHA
AANTKSAHPDDKKI

Sequences:

>Translated_574_residues
MIINRLFAWGVLAIRDFSDRRWWRWCCLALITASLILLSHPSRDVVVAQTLESETVEVDGIPLFELSSSKEFPAEDRART
VNQSLQTLVNTSTKAEVIVDFSKDLPVLKVEVGAKQPLYLLTVNSQDAELSSPEVTARTWQKKLQRAINRGQDERANPGR
LVAIGALCLLLALIAHWGIGWFWRQKLFKLLPRATVDPTTGEQPQSLRFLLKAIFYCLRIVLWVGTIGFVCGLFPWTRNG
IYLIINTLIQTFSTPFIPAGGKSYSVVDVALLLLLLAGLMNLTKTVQHLLRSRVLGATGLNRGAQEAVAFVANYVLLFIG
AMVLFQLWGFDLSSLAVFASVLGVGIGLGLQGFAKNFISGMVLIFEQPIKVGDFVKVGDYQGTVERINVRSTEIRTLDQV
SIIVPNSEFLESEVVNWDHGSSVSRLQIPVGVAYGTNPKKVREALMDAVAEYSSVRKDPPPRVFFTEFGDNSLNFLLLVW
IDEPEKQFAIKSDLNFRIETILRHREIEIPFPQRDLHVRSGKLPLDLPEELVTSFTLLSQNLNVWLQQQSLKENASDTHA
AANTKSAHPDDKKI
>Mature_574_residues
MIINRLFAWGVLAIRDFSDRRWWRWCCLALITASLILLSHPSRDVVVAQTLESETVEVDGIPLFELSSSKEFPAEDRART
VNQSLQTLVNTSTKAEVIVDFSKDLPVLKVEVGAKQPLYLLTVNSQDAELSSPEVTARTWQKKLQRAINRGQDERANPGR
LVAIGALCLLLALIAHWGIGWFWRQKLFKLLPRATVDPTTGEQPQSLRFLLKAIFYCLRIVLWVGTIGFVCGLFPWTRNG
IYLIINTLIQTFSTPFIPAGGKSYSVVDVALLLLLLAGLMNLTKTVQHLLRSRVLGATGLNRGAQEAVAFVANYVLLFIG
AMVLFQLWGFDLSSLAVFASVLGVGIGLGLQGFAKNFISGMVLIFEQPIKVGDFVKVGDYQGTVERINVRSTEIRTLDQV
SIIVPNSEFLESEVVNWDHGSSVSRLQIPVGVAYGTNPKKVREALMDAVAEYSSVRKDPPPRVFFTEFGDNSLNFLLLVW
IDEPEKQFAIKSDLNFRIETILRHREIEIPFPQRDLHVRSGKLPLDLPEELVTSFTLLSQNLNVWLQQQSLKENASDTHA
AANTKSAHPDDKKI

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=327, Percent_Identity=30.2752293577982, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI2367355, Length=262, Percent_Identity=31.2977099236641, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1789291, Length=275, Percent_Identity=28, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787591, Length=285, Percent_Identity=24.5614035087719, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 64150; Mature: 64150

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINRLFAWGVLAIRDFSDRRWWRWCCLALITASLILLSHPSRDVVVAQTLESETVEVDG
CCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEECC
IPLFELSSSKEFPAEDRARTVNQSLQTLVNTSTKAEVIVDFSKDLPVLKVEVGAKQPLYL
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCEEE
LTVNSQDAELSSPEVTARTWQKKLQRAINRGQDERANPGRLVAIGALCLLLALIAHWGIG
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCH
WFWRQKLFKLLPRATVDPTTGEQPQSLRFLLKAIFYCLRIVLWVGTIGFVCGLFPWTRNG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IYLIINTLIQTFSTPFIPAGGKSYSVVDVALLLLLLAGLMNLTKTVQHLLRSRVLGATGL
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NRGAQEAVAFVANYVLLFIGAMVLFQLWGFDLSSLAVFASVLGVGIGLGLQGFAKNFISG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
MVLIFEQPIKVGDFVKVGDYQGTVERINVRSTEIRTLDQVSIIVPNSEFLESEVVNWDHG
EEHHHCCCCCCCCEEEECCCCCCHHHEECCHHHHEEECEEEEEECCHHHHHHHHCCCCCC
SSVSRLQIPVGVAYGTNPKKVREALMDAVAEYSSVRKDPPPRVFFTEFGDNSLNFLLLVW
CCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEE
IDEPEKQFAIKSDLNFRIETILRHREIEIPFPQRDLHVRSGKLPLDLPEELVTSFTLLSQ
ECCCCHHEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
NLNVWLQQQSLKENASDTHAAANTKSAHPDDKKI
CHHHEEEHHHHHCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIINRLFAWGVLAIRDFSDRRWWRWCCLALITASLILLSHPSRDVVVAQTLESETVEVDG
CCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEECC
IPLFELSSSKEFPAEDRARTVNQSLQTLVNTSTKAEVIVDFSKDLPVLKVEVGAKQPLYL
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCEEE
LTVNSQDAELSSPEVTARTWQKKLQRAINRGQDERANPGRLVAIGALCLLLALIAHWGIG
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCH
WFWRQKLFKLLPRATVDPTTGEQPQSLRFLLKAIFYCLRIVLWVGTIGFVCGLFPWTRNG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IYLIINTLIQTFSTPFIPAGGKSYSVVDVALLLLLLAGLMNLTKTVQHLLRSRVLGATGL
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NRGAQEAVAFVANYVLLFIGAMVLFQLWGFDLSSLAVFASVLGVGIGLGLQGFAKNFISG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
MVLIFEQPIKVGDFVKVGDYQGTVERINVRSTEIRTLDQVSIIVPNSEFLESEVVNWDHG
EEHHHCCCCCCCCEEEECCCCCCHHHEECCHHHHEEECEEEEEECCHHHHHHHHCCCCCC
SSVSRLQIPVGVAYGTNPKKVREALMDAVAEYSSVRKDPPPRVFFTEFGDNSLNFLLLVW
CCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEE
IDEPEKQFAIKSDLNFRIETILRHREIEIPFPQRDLHVRSGKLPLDLPEELVTSFTLLSQ
ECCCCHHEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
NLNVWLQQQSLKENASDTHAAANTKSAHPDDKKI
CHHHEEEHHHHHCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]