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The map label for this gene is purF [H]

Identifier: 158318927

GI number: 158318927

Start: 8803104

End: 8804975

Strand: Reverse

Name: purF [H]

Synonym: Franean1_7210

Alternate gene names: 158318927

Gene position: 8804975-8803104 (Counterclockwise)

Preceding gene: 158318928

Following gene: 158318926

Centisome position: 98.03

GC content: 72.86

Gene sequence:

>1872_bases
GTGCCACACGTCGCCCACAGCGACGACCCCGCATCTCGCCGGGAGTCGACGTCCGTCCGCCGCCCGCCGCAGTCTCGCGA
CGGCCGCCGTCGCGACGGCCTTCTCCACGGTGCCCCGCGCAACGGTGATCCTCTCGACAGCGATCTTCTCGACGACGATC
CCGGCCCGCGCGACGCCTGTGGCGTCTTCGGTGTCTGGGCGCCCGGGGAGGACGTGGCGAACCTCACCTACTACGGCCTC
TACGCTCTCCAGCACCGCGGCCAGGAGGCCGCCGGGATGGCGGTCGGCGACGGCCGCACCGTCGTCGTGTTCAAGGAGCT
GGGTCTGGTCGCCCAGGTCTTCGACGAGATCACCCTCTCCAGCCTGTCGGGGCACGTGGCCGTCGGGCACACCCGGTACT
CGACCACAGGCTCGTCCACCTGGGAGAACGCCCAGCCGTCCTACCGGACGGCGGGTTTCGGCGGGCCGATCGCGCTCGGC
CACAACGGCAACCTGACGAACATCGTCGAGTTGGCCGGGTCGCTCGGCGACAGCGAGGGCGGCCAGGGCGCCACCACCGA
CTCCGACCTGATCACCGCGATGCTCGCCGCGCATCCTGGCCCGACCCTGGTCGACGCCGCGATGGACGTCCTGCCCCGGC
TGGCGGGGGCCTTCTCGCTGGCGTTCGCGGACGCGTCGACCCTGTACGCGGCGCGGGACCCGCACGGCATCCATCCACTG
GTGCTGGGCCGGCTCGACGACCGGCCGGACGGCGCGTGGATCGTCGCCAGCGAGACCGCGGCGCTCGACATCGTGGGCGC
CCGCTTCGTCCGCGAGATCGAGCCCGGTGAGCTGGTCGTCATCGACGCGGACGGCCCGCGCTCGCTCACGTTCGCGCAGG
CCGACCGGCACGTCTGCCTGTTCGAGTACGTCTACCTCGCCCGCCCGGACACGACGATCGGTGGCCGGTCGGTGCACGCC
ACCCGGGTCGAGGTGGGCCGGCGGCTCGCCCGGGAGGCACCGGTCGAGGCCGACCTGGTGATCCCGGTCCCGCAGTCCGG
TGTGCCGGCCGCGGTCGGCTTCGCCGAGGCGTCCGGCATCCCGTTCGGTGAGGGCCTGGTCAAGAACTCCTACGTCGGGC
GGACGTTCATCCAGCCGTCGCAGACCATCCGCCAGCGGGGCATCCGGCTCAAGCTGAACCCGTTGCGCGACGTCATCGAG
GGCCGCCGGCTCGTCGTCGTGGACGACTCGATCGTGCGCGGCAACACCCAGCGGGCCCTGGTGCGGATGCTGCGCGAGGC
CGGGGCGACCGAGGTGCACGTGCGGATCTCGTCGCCCCCGGTGCGGTGGCCCTGTTTCTACGGCATCGACTTCGCCACCC
GCGCCGAGCTGATCGCCAGCGGCTGTGGCGTGGAGGAGATCCGGCGCTCGCTGGGTGCCGACTCGCTCGCCTACGTCTCC
CTCGACGAGCTGGTGGAGGCCTCCGAGCAGCCGGCGGACGCGCTGTGCCGGGCCTGCTTCGACGGCATCTACCCGGTTCC
GCTCGCCGACTCGGACAAGCTGGGCAAGTACCGGCTGGAGCCGTTCGGGCCGCAGACCACGGAGGAGGTCATCGCGCAGG
CCCTCCGGGAGCGGGTAACCCTGGGTATGAACGGCGCGGACCCGTCCCCCGACAACCACGATCTCGACCTCGAACCCGTC
CCGCTCGAGCCCGCCCAGGACGTCGGCGCCGCGCGCGCGAACGGGGCACGGCCCGACGGGACGAGGGCCGACGCCGTGGC
GGTCGACGGCGCACCGGCCGACGGGGTGGCCACGAACGGAGCCATCGTGAACGGGATGACAGCGGACGGGGTGGCAGTGG
ACGGCGCACCGCTGGGCAGCGCGCGGTGGTGA

Upstream 100 bases:

>100_bases
TGATGTGAGTGTCGTCGTGCTCGGGGGTGTCACGACATCGCCTTGAACCCGCCCCGTTCCTCCCGACATCTCGGTCACCC
TGACCTCTCTAGGAGACCTG

Downstream 100 bases:

>100_bases
GGCACGCCAACGGATCCGGCCAGGGCTCAGAGGCCGGCGGCCAGGGGAGCACCACCGAGGGGCGCTCGGTCTCCTACCGG
GACGCCGGGGTCGACGTCGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MPHVAHSDDPASRRESTSVRRPPQSRDGRRRDGLLHGAPRNGDPLDSDLLDDDPGPRDACGVFGVWAPGEDVANLTYYGL
YALQHRGQEAAGMAVGDGRTVVVFKELGLVAQVFDEITLSSLSGHVAVGHTRYSTTGSSTWENAQPSYRTAGFGGPIALG
HNGNLTNIVELAGSLGDSEGGQGATTDSDLITAMLAAHPGPTLVDAAMDVLPRLAGAFSLAFADASTLYAARDPHGIHPL
VLGRLDDRPDGAWIVASETAALDIVGARFVREIEPGELVVIDADGPRSLTFAQADRHVCLFEYVYLARPDTTIGGRSVHA
TRVEVGRRLAREAPVEADLVIPVPQSGVPAAVGFAEASGIPFGEGLVKNSYVGRTFIQPSQTIRQRGIRLKLNPLRDVIE
GRRLVVVDDSIVRGNTQRALVRMLREAGATEVHVRISSPPVRWPCFYGIDFATRAELIASGCGVEEIRRSLGADSLAYVS
LDELVEASEQPADALCRACFDGIYPVPLADSDKLGKYRLEPFGPQTTEEVIAQALRERVTLGMNGADPSPDNHDLDLEPV
PLEPAQDVGAARANGARPDGTRADAVAVDGAPADGVATNGAIVNGMTADGVAVDGAPLGSARW

Sequences:

>Translated_623_residues
MPHVAHSDDPASRRESTSVRRPPQSRDGRRRDGLLHGAPRNGDPLDSDLLDDDPGPRDACGVFGVWAPGEDVANLTYYGL
YALQHRGQEAAGMAVGDGRTVVVFKELGLVAQVFDEITLSSLSGHVAVGHTRYSTTGSSTWENAQPSYRTAGFGGPIALG
HNGNLTNIVELAGSLGDSEGGQGATTDSDLITAMLAAHPGPTLVDAAMDVLPRLAGAFSLAFADASTLYAARDPHGIHPL
VLGRLDDRPDGAWIVASETAALDIVGARFVREIEPGELVVIDADGPRSLTFAQADRHVCLFEYVYLARPDTTIGGRSVHA
TRVEVGRRLAREAPVEADLVIPVPQSGVPAAVGFAEASGIPFGEGLVKNSYVGRTFIQPSQTIRQRGIRLKLNPLRDVIE
GRRLVVVDDSIVRGNTQRALVRMLREAGATEVHVRISSPPVRWPCFYGIDFATRAELIASGCGVEEIRRSLGADSLAYVS
LDELVEASEQPADALCRACFDGIYPVPLADSDKLGKYRLEPFGPQTTEEVIAQALRERVTLGMNGADPSPDNHDLDLEPV
PLEPAQDVGAARANGARPDGTRADAVAVDGAPADGVATNGAIVNGMTADGVAVDGAPLGSARW
>Mature_622_residues
PHVAHSDDPASRRESTSVRRPPQSRDGRRRDGLLHGAPRNGDPLDSDLLDDDPGPRDACGVFGVWAPGEDVANLTYYGLY
ALQHRGQEAAGMAVGDGRTVVVFKELGLVAQVFDEITLSSLSGHVAVGHTRYSTTGSSTWENAQPSYRTAGFGGPIALGH
NGNLTNIVELAGSLGDSEGGQGATTDSDLITAMLAAHPGPTLVDAAMDVLPRLAGAFSLAFADASTLYAARDPHGIHPLV
LGRLDDRPDGAWIVASETAALDIVGARFVREIEPGELVVIDADGPRSLTFAQADRHVCLFEYVYLARPDTTIGGRSVHAT
RVEVGRRLAREAPVEADLVIPVPQSGVPAAVGFAEASGIPFGEGLVKNSYVGRTFIQPSQTIRQRGIRLKLNPLRDVIEG
RRLVVVDDSIVRGNTQRALVRMLREAGATEVHVRISSPPVRWPCFYGIDFATRAELIASGCGVEEIRRSLGADSLAYVSL
DELVEASEQPADALCRACFDGIYPVPLADSDKLGKYRLEPFGPQTTEEVIAQALRERVTLGMNGADPSPDNHDLDLEPVP
LEPAQDVGAARANGARPDGTRADAVAVDGAPADGVATNGAIVNGMTADGVAVDGAPLGSARW

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=513, Percent_Identity=38.7914230019493, Blast_Score=351, Evalue=1e-96,
Organism=Escherichia coli, GI1788651, Length=480, Percent_Identity=39.7916666666667, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790167, Length=228, Percent_Identity=30.2631578947368, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=39.2484342379958, Blast_Score=303, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6323958, Length=463, Percent_Identity=35.2051835853132, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24659598, Length=491, Percent_Identity=40.122199592668, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=40.6060606060606, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24659604, Length=458, Percent_Identity=40.8296943231441, Blast_Score=345, Evalue=5e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 65825; Mature: 65694

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHVAHSDDPASRRESTSVRRPPQSRDGRRRDGLLHGAPRNGDPLDSDLLDDDPGPRDAC
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHCCCEECCCCCCCCCCCCCCCCCCCCCCCC
GVFGVWAPGEDVANLTYYGLYALQHRGQEAAGMAVGDGRTVVVFKELGLVAQVFDEITLS
CEEEECCCCCCHHHHHHHHHHHHHHCCCHHCCEEECCCCEEEEEHHHHHHHHHHHHHHHH
SLSGHVAVGHTRYSTTGSSTWENAQPSYRTAGFGGPIALGHNGNLTNIVELAGSLGDSEG
HCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCC
GQGATTDSDLITAMLAAHPGPTLVDAAMDVLPRLAGAFSLAFADASTLYAARDPHGIHPL
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCE
VLGRLDDRPDGAWIVASETAALDIVGARFVREIEPGELVVIDADGPRSLTFAQADRHVCL
EEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCEEE
FEYVYLARPDTTIGGRSVHATRVEVGRRLAREAPVEADLVIPVPQSGVPAAVGFAEASGI
EEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEHHHCCCC
PFGEGLVKNSYVGRTFIQPSQTIRQRGIRLKLNPLRDVIEGRRLVVVDDSIVRGNTQRAL
CCCCCHHCCCCCCCEECCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHHHCCCHHHHH
VRMLREAGATEVHVRISSPPVRWPCFYGIDFATRAELIASGCGVEEIRRSLGADSLAYVS
HHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEE
LDELVEASEQPADALCRACFDGIYPVPLADSDKLGKYRLEPFGPQTTEEVIAQALRERVT
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHE
LGMNGADPSPDNHDLDLEPVPLEPAQDVGAARANGARPDGTRADAVAVDGAPADGVATNG
ECCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHCCCCCCCCCCCCEEEECCCCCCCCCCCC
AIVNGMTADGVAVDGAPLGSARW
EEEECCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
PHVAHSDDPASRRESTSVRRPPQSRDGRRRDGLLHGAPRNGDPLDSDLLDDDPGPRDAC
CCCCCCCCCHHHHHCCCCCCCCCCCCCCHHCCCEECCCCCCCCCCCCCCCCCCCCCCCC
GVFGVWAPGEDVANLTYYGLYALQHRGQEAAGMAVGDGRTVVVFKELGLVAQVFDEITLS
CEEEECCCCCCHHHHHHHHHHHHHHCCCHHCCEEECCCCEEEEEHHHHHHHHHHHHHHHH
SLSGHVAVGHTRYSTTGSSTWENAQPSYRTAGFGGPIALGHNGNLTNIVELAGSLGDSEG
HCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCC
GQGATTDSDLITAMLAAHPGPTLVDAAMDVLPRLAGAFSLAFADASTLYAARDPHGIHPL
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCE
VLGRLDDRPDGAWIVASETAALDIVGARFVREIEPGELVVIDADGPRSLTFAQADRHVCL
EEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCEEE
FEYVYLARPDTTIGGRSVHATRVEVGRRLAREAPVEADLVIPVPQSGVPAAVGFAEASGI
EEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEHHHCCCC
PFGEGLVKNSYVGRTFIQPSQTIRQRGIRLKLNPLRDVIEGRRLVVVDDSIVRGNTQRAL
CCCCCHHCCCCCCCEECCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHHHCCCHHHHH
VRMLREAGATEVHVRISSPPVRWPCFYGIDFATRAELIASGCGVEEIRRSLGADSLAYVS
HHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEE
LDELVEASEQPADALCRACFDGIYPVPLADSDKLGKYRLEPFGPQTTEEVIAQALRERVT
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHE
LGMNGADPSPDNHDLDLEPVPLEPAQDVGAARANGARPDGTRADAVAVDGAPADGVATNG
ECCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHCCCCCCCCCCCCEEEECCCCCCCCCCCC
AIVNGMTADGVAVDGAPLGSARW
EEEECCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]