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The map label for this gene is mcrA [H]

Identifier: 158318903

GI number: 158318903

Start: 8773540

End: 8774961

Strand: Direct

Name: mcrA [H]

Synonym: Franean1_7186

Alternate gene names: 158318903

Gene position: 8773540-8774961 (Clockwise)

Preceding gene: 158318901

Following gene: 158318904

Centisome position: 97.68

GC content: 75.39

Gene sequence:

>1422_bases
ATGTCCTTCACGCCCGACTCCTCGTCCCTGCAGCTGCCGCTGGCCGACCTCGCCGGCAAGATCAGCGGCGATGTCCTGCT
GCCCGGCACCGCCGCCTACGCGGCGGCCGCCACCCCGTGGAACGTCGCCGTCGCCGTCTCCCCGGTGGCCGTGGTCGCGG
TCGCCGACGCGACCGACGTCGTCACCGCCGTCCGCTTCGCCGACGCCCACGACCTGGACGTCATCGTCCAGTCCACCGGC
CACGGCGTCCTCGCCCACACCCGGCCGAGCCTGCTCGTCCACACCGGTCGCCTCGACGAGGTCACCATCGACCCGGCCAC
CCGCCGGGCCCGCGTCGGCGCCGGGGTGCGCTGGCAGCGGGTGCTCGACGCCGCCGCCGAGCACGGCCTCGGCGCGCTCG
CCGGATCCGCGCCGCACGTGGGCGTCGTCGGGTACCTGACCGGCGGCGGCCTGTCGCCGGTCTCACGGACCTTCGGCTAC
GGAAGCGATCTCGTCACCGCCTTCGACGTCGTCACCGGGGACGGCGAGCTGCGCCGGGCCACCGCCACGGAGAACGCGGG
GCTGTTCTGGGCGCTGCGCGGCGGGAAGGGCGCGCTCGGCGTGGTCACCGCCGTCGAGTTCGAGCTGCTGGACGTCGACC
GGATCTACGGCGGCTGCCTCTACTTCGACGGCGCGGACGCCGACCGGGTCCTGTCTGCGTGGAAGACGTGGTCGGCGGCG
CTGCCGGAGCAGGCGTCGACGTCCATCGCGATCATGCGGCTGCCGGCGATGCCGGCGGTGCCCCCGCCGCTGGCGGACCG
GTGCACCGTCGCGGTCCGCTTCGCCTGGGTCGGGGACCCGGCCGACGGCGAGCGGGTGATCGATCCGATCCGCGGCCTCG
CACCGGCGATCTTCGGTGGGCTCGGCGTCATGCCGTTCGCCGCGCTCGGGATGATCCACGCCGACCCGGTCGACCCGATG
CCCGTCCACGAGCGGTCGCTGCTGCTGCGCGAGCTGCCGGACGCCGCGGTGCGCACCCTGCTCGCGCTGACCGGGCCGGC
CGCCGACTGCCCACAGGTGATCGTCGAGCTGCGGCTGCTCGGCGGCGCGCTGGCCCGTGAGCCCCGGGAGTCGAGCGCCG
TCTGCCACCGGGAGGCTGCCTACTCCCTGCTGACCATCGGCATCGCCGCCCCGCCGATCGTGGAGGCCACCGCCGGGCAC
GCCGCCGCGGTGATGGACGCGCTGCAGCCGTGGTCCGGTGGTATCTCGGGACCGAACTTCGGCGCCACCGCGGACCCGAC
GCTCGTCGCGCTCAAGTACGACATGGCCGCGCTGGCCCGCCTCGCCACGCTCACCGCCAGCTACGACCCGAACGCGCGGA
TGGCCGCCTCGCACCCGGTGCGGGCCGCCGCCGGTCTGGTGCAGTCGCGGGCACGGTCCTGA

Upstream 100 bases:

>100_bases
GGGGCGCAAGCGGGCGGCGGCGAACGACAAGTGGGGTCCAAGCGGCGGACCGCACGCTCATCTCGTCCCGTTCGACACCG
CTCGACCAGACGGAGCCGTC

Downstream 100 bases:

>100_bases
TCCCGCGTCCCGCGAGCCAGGGCCGCGAAGACGGCATCATGAGGGGCGTGCCGTACTACCGCCTGTTCGGGTCGATCGAG
GTCGTCCGGGACGGCCGGCC

Product: FAD linked oxidase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSFTPDSSSLQLPLADLAGKISGDVLLPGTAAYAAAATPWNVAVAVSPVAVVAVADATDVVTAVRFADAHDLDVIVQSTG
HGVLAHTRPSLLVHTGRLDEVTIDPATRRARVGAGVRWQRVLDAAAEHGLGALAGSAPHVGVVGYLTGGGLSPVSRTFGY
GSDLVTAFDVVTGDGELRRATATENAGLFWALRGGKGALGVVTAVEFELLDVDRIYGGCLYFDGADADRVLSAWKTWSAA
LPEQASTSIAIMRLPAMPAVPPPLADRCTVAVRFAWVGDPADGERVIDPIRGLAPAIFGGLGVMPFAALGMIHADPVDPM
PVHERSLLLRELPDAAVRTLLALTGPAADCPQVIVELRLLGGALAREPRESSAVCHREAAYSLLTIGIAAPPIVEATAGH
AAAVMDALQPWSGGISGPNFGATADPTLVALKYDMAALARLATLTASYDPNARMAASHPVRAAAGLVQSRARS

Sequences:

>Translated_473_residues
MSFTPDSSSLQLPLADLAGKISGDVLLPGTAAYAAAATPWNVAVAVSPVAVVAVADATDVVTAVRFADAHDLDVIVQSTG
HGVLAHTRPSLLVHTGRLDEVTIDPATRRARVGAGVRWQRVLDAAAEHGLGALAGSAPHVGVVGYLTGGGLSPVSRTFGY
GSDLVTAFDVVTGDGELRRATATENAGLFWALRGGKGALGVVTAVEFELLDVDRIYGGCLYFDGADADRVLSAWKTWSAA
LPEQASTSIAIMRLPAMPAVPPPLADRCTVAVRFAWVGDPADGERVIDPIRGLAPAIFGGLGVMPFAALGMIHADPVDPM
PVHERSLLLRELPDAAVRTLLALTGPAADCPQVIVELRLLGGALAREPRESSAVCHREAAYSLLTIGIAAPPIVEATAGH
AAAVMDALQPWSGGISGPNFGATADPTLVALKYDMAALARLATLTASYDPNARMAASHPVRAAAGLVQSRARS
>Mature_472_residues
SFTPDSSSLQLPLADLAGKISGDVLLPGTAAYAAAATPWNVAVAVSPVAVVAVADATDVVTAVRFADAHDLDVIVQSTGH
GVLAHTRPSLLVHTGRLDEVTIDPATRRARVGAGVRWQRVLDAAAEHGLGALAGSAPHVGVVGYLTGGGLSPVSRTFGYG
SDLVTAFDVVTGDGELRRATATENAGLFWALRGGKGALGVVTAVEFELLDVDRIYGGCLYFDGADADRVLSAWKTWSAAL
PEQASTSIAIMRLPAMPAVPPPLADRCTVAVRFAWVGDPADGERVIDPIRGLAPAIFGGLGVMPFAALGMIHADPVDPMP
VHERSLLLRELPDAAVRTLLALTGPAADCPQVIVELRLLGGALAREPRESSAVCHREAAYSLLTIGIAAPPIVEATAGHA
AAVMDALQPWSGGISGPNFGATADPTLVALKYDMAALARLATLTASYDPNARMAASHPVRAAAGLVQSRARS

Specific function: Involved in mitomycin resistance; oxidizes reduced form of mitomycins [H]

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012951
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR006093 [H]

Pfam domain/function: PF08031 BBE; PF01565 FAD_binding_4 [H]

EC number: NA

Molecular weight: Translated: 48696; Mature: 48565

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00862 OX2_COVAL_FAD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTPDSSSLQLPLADLAGKISGDVLLPGTAAYAAAATPWNVAVAVSPVAVVAVADATDV
CCCCCCCCCEEEEHHHHCCCCCCCEECCCCHHHHCCCCCCEEEEEECCEEEEEECCHHHH
VTAVRFADAHDLDVIVQSTGHGVLAHTRPSLLVHTGRLDEVTIDPATRRARVGAGVRWQR
HHHHHHCCCCCCEEEEECCCCEEEEECCCCEEEECCCCCEEEECCCHHHHHHCCCCHHHH
VLDAAAEHGLGALAGSAPHVGVVGYLTGGGLSPVSRTFGYGSDLVTAFDVVTGDGELRRA
HHHHHHHCCCEECCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCEEEEEEEECCCCEEEE
TATENAGLFWALRGGKGALGVVTAVEFELLDVDRIYGGCLYFDGADADRVLSAWKTWSAA
ECCCCCCEEEEEECCCCCEEEEEEEEEEEEEHHHHHCCEEEECCCCHHHHHHHHHHHHHC
LPEQASTSIAIMRLPAMPAVPPPLADRCTVAVRFAWVGDPADGERVIDPIRGLAPAIFGG
CCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LGVMPFAALGMIHADPVDPMPVHERSLLLRELPDAAVRTLLALTGPAADCPQVIVELRLL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
GGALAREPRESSAVCHREAAYSLLTIGIAAPPIVEATAGHAAAVMDALQPWSGGISGPNF
HHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCC
GATADPTLVALKYDMAALARLATLTASYDPNARMAASHPVRAAAGLVQSRARS
CCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFTPDSSSLQLPLADLAGKISGDVLLPGTAAYAAAATPWNVAVAVSPVAVVAVADATDV
CCCCCCCCEEEEHHHHCCCCCCCEECCCCHHHHCCCCCCEEEEEECCEEEEEECCHHHH
VTAVRFADAHDLDVIVQSTGHGVLAHTRPSLLVHTGRLDEVTIDPATRRARVGAGVRWQR
HHHHHHCCCCCCEEEEECCCCEEEEECCCCEEEECCCCCEEEECCCHHHHHHCCCCHHHH
VLDAAAEHGLGALAGSAPHVGVVGYLTGGGLSPVSRTFGYGSDLVTAFDVVTGDGELRRA
HHHHHHHCCCEECCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCEEEEEEEECCCCEEEE
TATENAGLFWALRGGKGALGVVTAVEFELLDVDRIYGGCLYFDGADADRVLSAWKTWSAA
ECCCCCCEEEEEECCCCCEEEEEEEEEEEEEHHHHHCCEEEECCCCHHHHHHHHHHHHHC
LPEQASTSIAIMRLPAMPAVPPPLADRCTVAVRFAWVGDPADGERVIDPIRGLAPAIFGG
CCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LGVMPFAALGMIHADPVDPMPVHERSLLLRELPDAAVRTLLALTGPAADCPQVIVELRLL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
GGALAREPRESSAVCHREAAYSLLTIGIAAPPIVEATAGHAAAVMDALQPWSGGISGPNF
HHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCC
GATADPTLVALKYDMAALARLATLTASYDPNARMAASHPVRAAAGLVQSRARS
CCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517396 [H]