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The map label for this gene is vanH [H]

Identifier: 158318857

GI number: 158318857

Start: 8718698

End: 8719738

Strand: Reverse

Name: vanH [H]

Synonym: Franean1_7138

Alternate gene names: 158318857

Gene position: 8719738-8718698 (Counterclockwise)

Preceding gene: 158318862

Following gene: 158318856

Centisome position: 97.08

GC content: 60.13

Gene sequence:

>1041_bases
ATGCCCCGCAGCGAACCCGCACGAACAGCGGCCCGCCGCACCCGGTCGCCGGCGCCCTCGTCCTCCTCAGCCGTCCCGGC
AACGGGAATCACGATCTATGGATGCGGGCAGGACGAGGCCGTTCTTTTCCGAGAGATGGCTCCCCGTTTCGGCGTACTGC
CAACCATCACAGACGTGGCGGTATCTGAAGCCAACATCGAACTGGCACTCGGAAACCGATGCATCAGCGTCGACCACAGG
ACTCGGGTCACGAGCTCCACTCTTCTTGCACTCAGCCAGGTCGGCGTGACGTACATCTCCACAAGAAGCATCGGGTGCAA
CCATATCGACGTGAAATACGCTGCGGGCGTCGGCATCTCCGTCGGAAACGTCGCCTATTCGCCCGACAGCGTGGCCGACT
ACACGCTGATGCTGATGCTGATGGTGGTGCGGAACGCAAAGTCCATCATCCGCCGCGCGGATATTCATGACTACAGACTG
AATGATGTGCGCGGGAGGGAACTACGCGATCTGACCATCGGGGTGGTTGGAACAGGACGCATCGGCGCGGCGGTCATGGA
CAGGCTGCGGGGTTTCGGCTGCCGAACACTGGCCTATGACGATCGCCCCGAGGCCGCCGCCGAATACGTTCCGCTCGACG
AATTGCTGGAACTGAGCGACATTGTGACGCTCCATACCCCCCTCAATGCGGATACACACCACCTCCTAAATCGTCGACGT
ATCGAGAGGATGAAGCGCGGCGCGTTCCTTGTCAATACCGGACGCGGCCCACTCCTTGATACCGAGGCCCTTGTTCAGGC
ATTGGAAAGCGGCAGACTGGGCGGTGCGGCGCTGGATGTCCTCGAAGGAGAGGAAGGAATATTCTACGCCGATCACAGGA
ACAAGCCCATCGAATGCGCACCGCTGCTACGGCTGCAAGAACTGCCGAATGTTCTGGTCAGTCCCCACACCGCCTACTAC
ACAGACCACGCCCTGCGTGACACCGTTGAAAACTCCATCACCAACTGCCTCGAATTCGAAAGCAGGATTCAGCATGGATA
G

Upstream 100 bases:

>100_bases
CTGCGGGTCATGCTCCCGTATGGTGCCGATCGGTTGCATATCGTGGGCATATTGAAAAACACATACGTGCCGGCAACACC
ACGCCGCCTTGGCTGGAGGC

Downstream 100 bases:

>100_bases
ACTGAGGATCGGAATCATATTCGGGGGCCGTTCCGAGGAGCATCCCGTCTCCGTCCGGTCTGCACAGGAGATCGCAAAGA
ACATCGATGCCGAGAAGTAC

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: Vancomycin resistance protein vanH [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MPRSEPARTAARRTRSPAPSSSSAVPATGITIYGCGQDEAVLFREMAPRFGVLPTITDVAVSEANIELALGNRCISVDHR
TRVTSSTLLALSQVGVTYISTRSIGCNHIDVKYAAGVGISVGNVAYSPDSVADYTLMLMLMVVRNAKSIIRRADIHDYRL
NDVRGRELRDLTIGVVGTGRIGAAVMDRLRGFGCRTLAYDDRPEAAAEYVPLDELLELSDIVTLHTPLNADTHHLLNRRR
IERMKRGAFLVNTGRGPLLDTEALVQALESGRLGGAALDVLEGEEGIFYADHRNKPIECAPLLRLQELPNVLVSPHTAYY
TDHALRDTVENSITNCLEFESRIQHG

Sequences:

>Translated_346_residues
MPRSEPARTAARRTRSPAPSSSSAVPATGITIYGCGQDEAVLFREMAPRFGVLPTITDVAVSEANIELALGNRCISVDHR
TRVTSSTLLALSQVGVTYISTRSIGCNHIDVKYAAGVGISVGNVAYSPDSVADYTLMLMLMVVRNAKSIIRRADIHDYRL
NDVRGRELRDLTIGVVGTGRIGAAVMDRLRGFGCRTLAYDDRPEAAAEYVPLDELLELSDIVTLHTPLNADTHHLLNRRR
IERMKRGAFLVNTGRGPLLDTEALVQALESGRLGGAALDVLEGEEGIFYADHRNKPIECAPLLRLQELPNVLVSPHTAYY
TDHALRDTVENSITNCLEFESRIQHG
>Mature_345_residues
PRSEPARTAARRTRSPAPSSSSAVPATGITIYGCGQDEAVLFREMAPRFGVLPTITDVAVSEANIELALGNRCISVDHRT
RVTSSTLLALSQVGVTYISTRSIGCNHIDVKYAAGVGISVGNVAYSPDSVADYTLMLMLMVVRNAKSIIRRADIHDYRLN
DVRGRELRDLTIGVVGTGRIGAAVMDRLRGFGCRTLAYDDRPEAAAEYVPLDELLELSDIVTLHTPLNADTHHLLNRRRI
ERMKRGAFLVNTGRGPLLDTEALVQALESGRLGGAALDVLEGEEGIFYADHRNKPIECAPLLRLQELPNVLVSPHTAYYT
DHALRDTVENSITNCLEFESRIQHG

Specific function: Required for high-level resistance to glycopeptides antibiotics. Catalyzes the reduction of 2-keto acids to 2-D- hydroxy acids that give rise to peptidoglycan precursors that terminate in the depsipeptide D-alanine-2-lactate rather than the dipeptide D-al

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=235, Percent_Identity=37.8723404255319, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557497, Length=235, Percent_Identity=37.8723404255319, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI145580578, Length=245, Percent_Identity=33.8775510204082, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557499, Length=245, Percent_Identity=33.8775510204082, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145580575, Length=246, Percent_Identity=34.9593495934959, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI23308577, Length=215, Percent_Identity=33.0232558139535, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI6912396, Length=281, Percent_Identity=30.2491103202847, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1787645, Length=318, Percent_Identity=32.3899371069182, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87082289, Length=237, Percent_Identity=32.4894514767932, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789279, Length=255, Percent_Identity=30.1960784313725, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788660, Length=199, Percent_Identity=32.1608040201005, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=256, Percent_Identity=29.296875, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25147481, Length=241, Percent_Identity=31.5352697095436, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=312, Percent_Identity=29.8076923076923, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=312, Percent_Identity=30.1282051282051, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=227, Percent_Identity=36.1233480176211, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324964, Length=208, Percent_Identity=31.7307692307692, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324980, Length=139, Percent_Identity=34.5323741007194, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62472511, Length=237, Percent_Identity=36.7088607594937, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646446, Length=248, Percent_Identity=35.8870967741936, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646448, Length=248, Percent_Identity=35.8870967741936, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646452, Length=248, Percent_Identity=35.8870967741936, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646450, Length=248, Percent_Identity=35.8870967741936, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28574286, Length=252, Percent_Identity=32.1428571428571, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28574284, Length=251, Percent_Identity=30.6772908366534, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI45551003, Length=251, Percent_Identity=30.6772908366534, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585514, Length=251, Percent_Identity=30.6772908366534, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28574282, Length=251, Percent_Identity=30.6772908366534, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI45552429, Length=235, Percent_Identity=31.4893617021277, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585516, Length=263, Percent_Identity=28.5171102661597, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19921140, Length=173, Percent_Identity=33.5260115606936, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571528, Length=232, Percent_Identity=30.6034482758621, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 37816; Mature: 37684

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSEPARTAARRTRSPAPSSSSAVPATGITIYGCGQDEAVLFREMAPRFGVLPTITDVA
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHH
VSEANIELALGNRCISVDHRTRVTSSTLLALSQVGVTYISTRSIGCNHIDVKYAAGVGIS
CCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEECCCCEE
VGNVAYSPDSVADYTLMLMLMVVRNAKSIIRRADIHDYRLNDVRGRELRDLTIGVVGTGR
ECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCC
IGAAVMDRLRGFGCRTLAYDDRPEAAAEYVPLDELLELSDIVTLHTPLNADTHHLLNRRR
HHHHHHHHHHCCCCEEECCCCCCCHHHHCCCHHHHHHHHHEEEEECCCCCHHHHHHHHHH
IERMKRGAFLVNTGRGPLLDTEALVQALESGRLGGAALDVLEGEEGIFYADHRNKPIECA
HHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCCHH
PLLRLQELPNVLVSPHTAYYTDHALRDTVENSITNCLEFESRIQHG
HHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRSEPARTAARRTRSPAPSSSSAVPATGITIYGCGQDEAVLFREMAPRFGVLPTITDVA
CCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHH
VSEANIELALGNRCISVDHRTRVTSSTLLALSQVGVTYISTRSIGCNHIDVKYAAGVGIS
CCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEECCCCEE
VGNVAYSPDSVADYTLMLMLMVVRNAKSIIRRADIHDYRLNDVRGRELRDLTIGVVGTGR
ECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCC
IGAAVMDRLRGFGCRTLAYDDRPEAAAEYVPLDELLELSDIVTLHTPLNADTHHLLNRRR
HHHHHHHHHHCCCCEEECCCCCCCHHHHCCCHHHHHHHHHEEEEECCCCCHHHHHHHHHH
IERMKRGAFLVNTGRGPLLDTEALVQALESGRLGGAALDVLEGEEGIFYADHRNKPIECA
HHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCCHH
PLLRLQELPNVLVSPHTAYYTDHALRDTVENSITNCLEFESRIQHG
HHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1908809; 8380148; 1931965; 1522072; 1556077 [H]