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The map label for this gene is vanB [C]

Identifier: 158318856

GI number: 158318856

Start: 8717659

End: 8718705

Strand: Reverse

Name: vanB [C]

Synonym: Franean1_7137

Alternate gene names: 158318856

Gene position: 8718705-8717659 (Counterclockwise)

Preceding gene: 158318857

Following gene: 158318855

Centisome position: 97.07

GC content: 60.36

Gene sequence:

>1047_bases
ATGGATAGACTGAGGATCGGAATCATATTCGGGGGCCGTTCCGAGGAGCATCCCGTCTCCGTCCGGTCTGCACAGGAGAT
CGCAAAGAACATCGATGCCGAGAAGTACGAACCGTTCTACATCGGGATCGCGAGGAGCGGTGCCTGGAAGCTGTGCGAAC
GCCCTGACGAAAATTGGGAGAACGGCAGCTGCCGTCCGGCTGTGCTGTCACCGGACAGAAGCGTCCACGGGTTGCTGGTT
CTGGAGCAGGGAAGATACGAAACGATCAGCCTGGACCTGGCGTTGCCCGTTCTGCACGGCAGACTCGGCGAGGACGGTGC
GATGCAGGGTCTGCTGGAGCTCTCCGGCATCCCCTACGTGGGCTGCGATGTCCAGAGTTCCGCTTTGTGCATGGACAAGT
CCCTTGCCTACACCGTCGCCAGAGGCGCGGGAATTGCTACGCCGAATTTCTGGACCGTGACGGCGAACGAGAAAGTGGAT
GCCGACCTGCTCACCTATCCCGTATTCGTGAAGCCGGCCCGTTCGGGGTCATCCTTCGGCGTCAGCAAGGTGTCTCGAAA
AGAAGAGCTGTCGGGTGCAGTGGATACCGCACGACAGTACGACTCGAAGGTGCTGATCGAAGAGGCTGTCGTCGGCAGCG
AGGTCGGATGTGCCCTGCTGGGGAACGATCTGGATCTGACCGCGGGCGAGGTAGATCGAATCTCGCTGTCTCATGGATTT
TTCAGGATACATCAGGAGAGTGCGCCTGAAAGCGGTTCCGAGAACTCGACGGCAATCGTTCCAGCGGACATCTCGGCAGA
GGCGCGCTCGCTCGTTCAGGAGACGGCAAAGGCCGTCTATCGCGCCCTGGGATGCAGGGGCCTGTCGCGGGTGGACATGT
TCCTGCAGGAAGACGGGAAAGTAGTCCTCAACGAGGTAAACACCCTGCCCGGCATGACATCGTACAGCCGTTATCCGAGA
ATGATGGCGGCCGCGGGGCTGCCGCTCGCCGAGGTGATCGACCGGCTGGTGTCGCTGACATTGGCGGGAAAAGTCGATGA
AGGATGA

Upstream 100 bases:

>100_bases
CTGGTCAGTCCCCACACCGCCTACTACACAGACCACGCCCTGCGTGACACCGTTGAAAACTCCATCACCAACTGCCTCGA
ATTCGAAAGCAGGATTCAGC

Downstream 100 bases:

>100_bases
CTTCGTCTTTGTGGACGAGTTTGTACCCGGAATACGCTGGGATGCCAAGTACGCCACCTGGGACAACTTCACCGGCAGAC
CGGTGAACGGATACCTGGCA

Product: D-alanine--D-lactate ligase

Products: ADP; phosphate; D-alanyl-D-alanine

Alternate protein names: Vancomycin resistance-like protein [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDRLRIGIIFGGRSEEHPVSVRSAQEIAKNIDAEKYEPFYIGIARSGAWKLCERPDENWENGSCRPAVLSPDRSVHGLLV
LEQGRYETISLDLALPVLHGRLGEDGAMQGLLELSGIPYVGCDVQSSALCMDKSLAYTVARGAGIATPNFWTVTANEKVD
ADLLTYPVFVKPARSGSSFGVSKVSRKEELSGAVDTARQYDSKVLIEEAVVGSEVGCALLGNDLDLTAGEVDRISLSHGF
FRIHQESAPESGSENSTAIVPADISAEARSLVQETAKAVYRALGCRGLSRVDMFLQEDGKVVLNEVNTLPGMTSYSRYPR
MMAAAGLPLAEVIDRLVSLTLAGKVDEG

Sequences:

>Translated_348_residues
MDRLRIGIIFGGRSEEHPVSVRSAQEIAKNIDAEKYEPFYIGIARSGAWKLCERPDENWENGSCRPAVLSPDRSVHGLLV
LEQGRYETISLDLALPVLHGRLGEDGAMQGLLELSGIPYVGCDVQSSALCMDKSLAYTVARGAGIATPNFWTVTANEKVD
ADLLTYPVFVKPARSGSSFGVSKVSRKEELSGAVDTARQYDSKVLIEEAVVGSEVGCALLGNDLDLTAGEVDRISLSHGF
FRIHQESAPESGSENSTAIVPADISAEARSLVQETAKAVYRALGCRGLSRVDMFLQEDGKVVLNEVNTLPGMTSYSRYPR
MMAAAGLPLAEVIDRLVSLTLAGKVDEG
>Mature_348_residues
MDRLRIGIIFGGRSEEHPVSVRSAQEIAKNIDAEKYEPFYIGIARSGAWKLCERPDENWENGSCRPAVLSPDRSVHGLLV
LEQGRYETISLDLALPVLHGRLGEDGAMQGLLELSGIPYVGCDVQSSALCMDKSLAYTVARGAGIATPNFWTVTANEKVD
ADLLTYPVFVKPARSGSSFGVSKVSRKEELSGAVDTARQYDSKVLIEEAVVGSEVGCALLGNDLDLTAGEVDRISLSHGF
FRIHQESAPESGSENSTAIVPADISAEARSLVQETAKAVYRALGCRGLSRVDMFLQEDGKVVLNEVNTLPGMTSYSRYPR
MMAAAGLPLAEVIDRLVSLTLAGKVDEG

Specific function: Required for resistance to glycopeptides antibiotics. D- Ala--D-Ala ligase of altered specificity which catalyzes ester bond formation between D-Ala and various D-hydroxy acids; producing a peptidoglycan which does not terminate by D-alanine but by D-lact

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=356, Percent_Identity=37.3595505617978, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1786280, Length=251, Percent_Identity=36.6533864541833, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: 6.3.2.4

Molecular weight: Translated: 37466; Mature: 37466

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLRIGIIFGGRSEEHPVSVRSAQEIAKNIDAEKYEPFYIGIARSGAWKLCERPDENWE
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCCCC
NGSCRPAVLSPDRSVHGLLVLEQGRYETISLDLALPVLHGRLGEDGAMQGLLELSGIPYV
CCCCCCEEECCCCCEEEEEEEECCCEEEEEEEEEEHHHHCCCCCCHHHHHHHHHCCCCEE
GCDVQSSALCMDKSLAYTVARGAGIATPNFWTVTANEKVDADLLTYPVFVKPARSGSSFG
CCCCCCCHHHHCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHEEEEEEEEECCCCCCCC
VSKVSRKEELSGAVDTARQYDSKVLIEEAVVGSEVGCALLGNDLDLTAGEVDRISLSHGF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCE
FRIHQESAPESGSENSTAIVPADISAEARSLVQETAKAVYRALGCRGLSRVDMFLQEDGK
EEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
VVLNEVNTLPGMTSYSRYPRMMAAAGLPLAEVIDRLVSLTLAGKVDEG
EEEEHHHCCCCCCHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDRLRIGIIFGGRSEEHPVSVRSAQEIAKNIDAEKYEPFYIGIARSGAWKLCERPDENWE
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCCCC
NGSCRPAVLSPDRSVHGLLVLEQGRYETISLDLALPVLHGRLGEDGAMQGLLELSGIPYV
CCCCCCEEECCCCCEEEEEEEECCCEEEEEEEEEEHHHHCCCCCCHHHHHHHHHCCCCEE
GCDVQSSALCMDKSLAYTVARGAGIATPNFWTVTANEKVDADLLTYPVFVKPARSGSSFG
CCCCCCCHHHHCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHEEEEEEEEECCCCCCCC
VSKVSRKEELSGAVDTARQYDSKVLIEEAVVGSEVGCALLGNDLDLTAGEVDRISLSHGF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCE
FRIHQESAPESGSENSTAIVPADISAEARSLVQETAKAVYRALGCRGLSRVDMFLQEDGK
EEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
VVLNEVNTLPGMTSYSRYPRMMAAAGLPLAEVIDRLVSLTLAGKVDEG
EEEEHHHCCCCCCHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-alanine

Specific reaction: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: (3-Amino-2-oxoalkyl) phosphonic acids; alpha-Aminophosphonamidic acid; D-(1-Aminoethyl) phosphonic acid in presence of ATP; D-Cycloserine; Tabtoxinine [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]