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The map label for this gene is mtlF [H]

Identifier: 158318799

GI number: 158318799

Start: 8631089

End: 8631583

Strand: Reverse

Name: mtlF [H]

Synonym: Franean1_7071

Alternate gene names: 158318799

Gene position: 8631583-8631089 (Counterclockwise)

Preceding gene: 158318800

Following gene: 158318798

Centisome position: 96.1

GC content: 71.72

Gene sequence:

>495_bases
ATGTCTGAGGGACACGGTGCGGCGCGGCAGGACAGCCCCGGCGGCACCATGAGCCGGCTGCTCGACCCGAGCGCGGTGCG
CCTCGCCGAGGTGGCAGCGAGCCGGGAGGATGCGATCCGCGGCTGCGGTCGCGCGCTGGTCGACGTCGGAGCGGTCGTCC
CGTCCTACATCGACGCCATGCTCGAGCGGGAGCGGACCATCTCCACCTATGTCGGCGAGGGGGTGGCGATCCCGCACGGC
ACCTTCGCCGGCAAGGAGCTGGTCCACCGGGACGCACTGGCGTTCCTGCGATTCCCGGACGGCGTCGACTGGGACGGCGA
AACGGTCCGGGTCTGTGTCGGCATCGCCGCCCGGGGCGACGGCCATGTGGCGCTGCTGTCCGAACTGGCCATGATCCTGC
TCGACCCGGACCGCGCCCGGGCGTTGCGCGAGGCAAGCGACGTCGACGACGTCATCCGGCTACTCCAGCCGGCTACCGGA
GAGGACTCCGCATGA

Upstream 100 bases:

>100_bases
CGGGGGGTGCCGGACAAGCCGATCGTGACATTCCAGGTGTTCATAGGTGATCCGGCCGTGGCCAACGTGCTGGCCGCCAT
CCGCGAGGGGAAAGTGCTCG

Downstream 100 bases:

>100_bases
AAGTGGCCCGGTTCTACGCGCCCGGGGACATCCGGATCGAAGAAGCGTCCGAGCCCGTGGCGGGCCCGGACGAGGTGAAA
GTCCGCGTCCGGAACTGCTC

Product: phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MSEGHGAARQDSPGGTMSRLLDPSAVRLAEVAASREDAIRGCGRALVDVGAVVPSYIDAMLERERTISTYVGEGVAIPHG
TFAGKELVHRDALAFLRFPDGVDWDGETVRVCVGIAARGDGHVALLSELAMILLDPDRARALREASDVDDVIRLLQPATG
EDSA

Sequences:

>Translated_164_residues
MSEGHGAARQDSPGGTMSRLLDPSAVRLAEVAASREDAIRGCGRALVDVGAVVPSYIDAMLERERTISTYVGEGVAIPHG
TFAGKELVHRDALAFLRFPDGVDWDGETVRVCVGIAARGDGHVALLSELAMILLDPDRARALREASDVDDVIRLLQPATG
EDSA
>Mature_163_residues
SEGHGAARQDSPGGTMSRLLDPSAVRLAEVAASREDAIRGCGRALVDVGAVVPSYIDAMLERERTISTYVGEGVAIPHGT
FAGKELVHRDALAFLRFPDGVDWDGETVRVCVGIAARGDGHVALLSELAMILLDPDRARALREASDVDDVIRLLQPATGE
DSA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=127, Percent_Identity=40.1574803149606, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1790027, Length=133, Percent_Identity=38.3458646616541, Blast_Score=97, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17348; Mature: 17217

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGHGAARQDSPGGTMSRLLDPSAVRLAEVAASREDAIRGCGRALVDVGAVVPSYIDAM
CCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERERTISTYVGEGVAIPHGTFAGKELVHRDALAFLRFPDGVDWDGETVRVCVGIAARGD
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCC
GHVALLSELAMILLDPDRARALREASDVDDVIRLLQPATGEDSA
CHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEGHGAARQDSPGGTMSRLLDPSAVRLAEVAASREDAIRGCGRALVDVGAVVPSYIDAM
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERERTISTYVGEGVAIPHGTFAGKELVHRDALAFLRFPDGVDWDGETVRVCVGIAARGD
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCC
GHVALLSELAMILLDPDRARALREASDVDDVIRLLQPATGEDSA
CHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA