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The map label for this gene is gutB [H]

Identifier: 158318798

GI number: 158318798

Start: 8630052

End: 8631092

Strand: Reverse

Name: gutB [H]

Synonym: Franean1_7070

Alternate gene names: 158318798

Gene position: 8631092-8630052 (Counterclockwise)

Preceding gene: 158318799

Following gene: 158318797

Centisome position: 96.09

GC content: 70.51

Gene sequence:

>1041_bases
ATGAAAGTGGCCCGGTTCTACGCGCCCGGGGACATCCGGATCGAAGAAGCGTCCGAGCCCGTGGCGGGCCCGGACGAGGT
GAAAGTCCGCGTCCGGAACTGCTCCACCTGCGGGACGGACGTCAAGATCTCGAAATTTGGCCACCATCACATCCGCCCGC
CCCGGGTGATGGGGCACGAGGTCGCCGGCGAGATCGTGGACGTCGGTGACCGGGTCGAGGGCTGGCAGGTCGGTGACCGG
GTTCAGGTGATCGCGGCGATCCCGTGCGGGCGGTGCGACGAGTGCCGGCGCGGCCGGATGACGGTCTGCCCCAACCAGGA
GTCCATCGGATACCACTACGATGGCGGCTTCGCCGAGTACGTGACCGTTCCCGGCAAGGTCCTCGCCGTGGACGGGCTGA
ACCGGATCCCGGAGGGGGTCGGATTCGCGGAGGCGTCGGTGGCCGAGCCGCTCGCGTGCGTCCTGAACGGGCAGGAACTC
GCCCGCGTCGGGGCGGGGGACGACGTCGTCATCATCGGGTCCGGGCCGATCGGCTGCCTGCACGTGCGGCTCGCCCGCTC
GCGCGGCGCCGGCCGGGTGTTCCTCGTCGAGCTCAACCGGGAGCGGCTGGACCGCGCCGCCGCACTGGTGGAGCCCGACG
CGAGCATCTGTGCCGCCGAAACCGACGCGGTCGACGCGGTCAGCAAGCTCACCGACGGCCGGGGCGCCGACGTCGTCATC
ACCGCCGCCGCGTCGGGAGCCGCGCAGGAACAGGCACTGCTGATGGCGGCCAGGCAGGGCCGGATCAGCTTCTTCGGGGG
GCTGCCGAAGGACAATCCGGTCATCGCCTGCGACTCGAACCTGGTCCACTACCGGGAGCTCACCATCGTCGGCGCCAACG
GCTCCAGCCCTGCGCACAACGCGCGGGCGTTGCAACACATCGCCACCGGCGACGTGCCGGTGTCCGATCTGATCACCCAC
CGGCTCGCCCTCACCGACGTGCTGGCGGGCATCGACATCGTCAGCCGCGGCCAGGGCATCAAGATCACGATCGAGCCCTG
A

Upstream 100 bases:

>100_bases
TGATCCTGCTCGACCCGGACCGCGCCCGGGCGTTGCGCGAGGCAAGCGACGTCGACGACGTCATCCGGCTACTCCAGCCG
GCTACCGGAGAGGACTCCGC

Downstream 100 bases:

>100_bases
CCGGCGGGCCGCCGGCGACCACGATTTCAAACTGCCGGCTCCGGTTGCCGGCAGTCTGAGGCGATCCAGTCTTCGAGGAG
ATGAAGAGCATGGCTTGTAG

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKVARFYAPGDIRIEEASEPVAGPDEVKVRVRNCSTCGTDVKISKFGHHHIRPPRVMGHEVAGEIVDVGDRVEGWQVGDR
VQVIAAIPCGRCDECRRGRMTVCPNQESIGYHYDGGFAEYVTVPGKVLAVDGLNRIPEGVGFAEASVAEPLACVLNGQEL
ARVGAGDDVVIIGSGPIGCLHVRLARSRGAGRVFLVELNRERLDRAAALVEPDASICAAETDAVDAVSKLTDGRGADVVI
TAAASGAAQEQALLMAARQGRISFFGGLPKDNPVIACDSNLVHYRELTIVGANGSSPAHNARALQHIATGDVPVSDLITH
RLALTDVLAGIDIVSRGQGIKITIEP

Sequences:

>Translated_346_residues
MKVARFYAPGDIRIEEASEPVAGPDEVKVRVRNCSTCGTDVKISKFGHHHIRPPRVMGHEVAGEIVDVGDRVEGWQVGDR
VQVIAAIPCGRCDECRRGRMTVCPNQESIGYHYDGGFAEYVTVPGKVLAVDGLNRIPEGVGFAEASVAEPLACVLNGQEL
ARVGAGDDVVIIGSGPIGCLHVRLARSRGAGRVFLVELNRERLDRAAALVEPDASICAAETDAVDAVSKLTDGRGADVVI
TAAASGAAQEQALLMAARQGRISFFGGLPKDNPVIACDSNLVHYRELTIVGANGSSPAHNARALQHIATGDVPVSDLITH
RLALTDVLAGIDIVSRGQGIKITIEP
>Mature_346_residues
MKVARFYAPGDIRIEEASEPVAGPDEVKVRVRNCSTCGTDVKISKFGHHHIRPPRVMGHEVAGEIVDVGDRVEGWQVGDR
VQVIAAIPCGRCDECRRGRMTVCPNQESIGYHYDGGFAEYVTVPGKVLAVDGLNRIPEGVGFAEASVAEPLACVLNGQEL
ARVGAGDDVVIIGSGPIGCLHVRLARSRGAGRVFLVELNRERLDRAAALVEPDASICAAETDAVDAVSKLTDGRGADVVI
TAAASGAAQEQALLMAARQGRISFFGGLPKDNPVIACDSNLVHYRELTIVGANGSSPAHNARALQHIATGDVPVSDLITH
RLALTDVLAGIDIVSRGQGIKITIEP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=352, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4501939, Length=231, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=23.0352303523035, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790045, Length=345, Percent_Identity=28.4057971014493, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788073, Length=332, Percent_Identity=28.6144578313253, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788075, Length=354, Percent_Identity=27.4011299435028, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI226510992, Length=329, Percent_Identity=26.4437689969605, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082125, Length=353, Percent_Identity=28.0453257790368, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788407, Length=292, Percent_Identity=29.4520547945205, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787863, Length=339, Percent_Identity=28.023598820059, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786825, Length=289, Percent_Identity=27.681660899654, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI48994910, Length=388, Percent_Identity=27.0618556701031, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790718, Length=184, Percent_Identity=28.804347826087, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081918, Length=213, Percent_Identity=27.6995305164319, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17562878, Length=217, Percent_Identity=30.8755760368664, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17562876, Length=270, Percent_Identity=28.5185185185185, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71988145, Length=312, Percent_Identity=26.9230769230769, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=313, Percent_Identity=27.4760383386581, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17562584, Length=315, Percent_Identity=27.3015873015873, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=348, Percent_Identity=31.3218390804598, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319955, Length=348, Percent_Identity=31.0344827586207, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6323099, Length=350, Percent_Identity=26, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323961, Length=325, Percent_Identity=28.9230769230769, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319621, Length=339, Percent_Identity=27.4336283185841, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319258, Length=368, Percent_Identity=24.7282608695652, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324486, Length=316, Percent_Identity=30.6962025316456, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319257, Length=266, Percent_Identity=27.4436090225564, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323729, Length=213, Percent_Identity=30.5164319248826, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=276, Percent_Identity=25.7246376811594, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17737897, Length=343, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI17137530, Length=203, Percent_Identity=31.5270935960591, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI221457811, Length=203, Percent_Identity=26.1083743842365, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550770, Length=203, Percent_Identity=26.1083743842365, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45551930, Length=203, Percent_Identity=26.1083743842365, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36497; Mature: 36497

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVARFYAPGDIRIEEASEPVAGPDEVKVRVRNCSTCGTDVKISKFGHHHIRPPRVMGHE
CCEEEEECCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCHHHCHH
VAGEIVDVGDRVEGWQVGDRVQVIAAIPCGRCDECRRGRMTVCPNQESIGYHYDGGFAEY
HHCHHCCCCCCCCCEECCCEEEEEEECCCCCCHHHHCCCEEECCCCCCCCEEECCCEEEE
VTVPGKVLAVDGLNRIPEGVGFAEASVAEPLACVLNGQELARVGAGDDVVIIGSGPIGCL
EECCCEEEEECCCHHCCCCCCCCHHHHHCCCEEEECCHHHHHCCCCCCEEEECCCCCEEE
HVRLARSRGAGRVFLVELNRERLDRAAALVEPDASICAAETDAVDAVSKLTDGRGADVVI
EEEEECCCCCCEEEEEEECHHHHHHHHHEECCCCCEEECCCHHHHHHHHHCCCCCCCEEE
TAAASGAAQEQALLMAARQGRISFFGGLPKDNPVIACDSNLVHYRELTIVGANGSSPAHN
EECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCEEEEEEEEEEECCCCCCCHH
ARALQHIATGDVPVSDLITHRLALTDVLAGIDIVSRGQGIKITIEP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCEEEEECC
>Mature Secondary Structure
MKVARFYAPGDIRIEEASEPVAGPDEVKVRVRNCSTCGTDVKISKFGHHHIRPPRVMGHE
CCEEEEECCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCHHHCHH
VAGEIVDVGDRVEGWQVGDRVQVIAAIPCGRCDECRRGRMTVCPNQESIGYHYDGGFAEY
HHCHHCCCCCCCCCEECCCEEEEEEECCCCCCHHHHCCCEEECCCCCCCCEEECCCEEEE
VTVPGKVLAVDGLNRIPEGVGFAEASVAEPLACVLNGQELARVGAGDDVVIIGSGPIGCL
EECCCEEEEECCCHHCCCCCCCCHHHHHCCCEEEECCHHHHHCCCCCCEEEECCCCCEEE
HVRLARSRGAGRVFLVELNRERLDRAAALVEPDASICAAETDAVDAVSKLTDGRGADVVI
EEEEECCCCCCEEEEEEECHHHHHHHHHEECCCCCEEECCCHHHHHHHHHCCCCCCCEEE
TAAASGAAQEQALLMAARQGRISFFGGLPKDNPVIACDSNLVHYRELTIVGANGSSPAHN
EECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCEEEEEEEEEEECCCCCCCHH
ARALQHIATGDVPVSDLITHRLALTDVLAGIDIVSRGQGIKITIEP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]