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The map label for this gene is ptsI [H]

Identifier: 158318797

GI number: 158318797

Start: 8627887

End: 8629962

Strand: Reverse

Name: ptsI [H]

Synonym: Franean1_7069

Alternate gene names: 158318797

Gene position: 8629962-8627887 (Counterclockwise)

Preceding gene: 158318798

Following gene: 158318796

Centisome position: 96.08

GC content: 73.46

Gene sequence:

>2076_bases
ATGGCTTGTAGGACGGCGACCGTGAATTCCCGCGGCGGGTTACACGCCCGCCCCGCAGCGCTCTTCGTCCGGGCCGCCGC
GCAGCAGGCGGTCCCGGTTCGGATCCGCAAGGGCGACGGCCCGGCCGTGAACGCCGCCAGCATGCTGTCGGTGCTGGCCC
TGGGCGCGATGTACGGAACGGTCGTGACGCTGGAAGCGGACGGTGAGCGGGCCGAGGAGGCGCTCGATGCTCTCGCCGCG
ATCCTTGCCCACGACCAGGACGCCGCCGACAGCGGGCAAGGAGACGGGCGGGTCGGGAGCGCCAGCGCGTCCGACAAGGC
GTTAGACGAGACGGGCGGGACCGGCAGGAGCCGGGCGACGGCTGACGTCTTCGTCGGACTCGGTGTCAGCCCCGGGCTGG
TCACCGGTCCCGCCTTCCGGATGGCGCGTCAACCCCGGCTGCCCGACCCGCGCCTCGTCCTCGATCCCGACGAGGAGGCC
GCTGCCGCCGCGCGGGTACTCCGGAACGTGGCCTCCGACCTGCGGGCCCACGCAGCGGCGACCCGGCTCTCCGCTGCCTC
GGACATTGTGACGGCACAGGCGATGATGGCGGAAGACCCGGTGCTCCTCGACGAGGTCGGCATGCGGGTCCGGTCGGGTC
TGGACGCCGTGCACGCGATCGATGCCGCACTTGCCGACCAGCGCCGTCAGCTGGAAGGAGCTGGCGGCTACCACGCCGAG
CGGGCCGCCGACATCGACGACATCCGGCATCGTGCCGTCGCGGCTCTGCTCGGCCTGCCCGCGCCCGGCCTCCCCGCGCC
CGGGTTCCCGTTCGTCCTGGTGGCCGAGGACCTCGCACCCGCCGACACCGCCACCCTCGACACCGATCTTGTCCTGGCCC
TGGTGACCGAGCGTGGCGGTCCCACCAGCCACACCGCCATCCTCGCCCGTGCCCTCGGGCTCCCCGCCGTCGTGTCCTGC
CCAGGCGCGATGGCCCTCGACGACGGGACCCCTGTCCGGGTCGACGGCACCACCGGCGAGGTCCACGTCGGGGTCGGGGT
CGATGTGGAGGCCGACGGCGCGGGCCACGGCGAGGCCATCGCGCAGGACACGGCCGATGTCCGCCAGCCCGGCTTGTGGC
GGACGACGTCGCGGGCCGCGAAGGCCGGCCCGGCCCGGGGTGGTCCGGGACGCACCGCGGACGGCCGCCCGGTCCAGCTG
CTCCTCAACATCGGCTCCGCCAAGGACCTGCGCGGCGACGTCGCCGCCACGGCGGAGGGCGTGGGCCTGTTCCGCACCGA
GTTCCTCTTCCTCAACCGGCGGGTCGCCCCCACTCCTGACGAGCAACGGGACGCCTACCAGGCCGTCTTCCGTGCAGCCG
GCAGCAGGAAGGTCGTCGTCCGCACGCTGGACGCCGGCGCCGACAAGCCGCTGCCCTTCCTGAGCCTGCCTGACGAGCCC
AACCCGGCGCTCGGCGTGCGGGGCTACCGGACGGTCTGGCTGCGCCCCGAGGTACTCGACACCCAACTGGGCGCGATCGC
GGACGCCGCGGCTGCGTGCGATGCGGACGTCTGGGTGATGGCGCCCATGGTTTCGACGCCCCCGGAGGCGGAGGCGTTCG
CGGCGGCCGCTCGCGGGCACGGGCTGGCAACGACTGGCGTCATGGTCGAGGTGCCTGCGGCGGCCCTGCGAGCTGGCCGG
ATGCTGGACACCGTCGACTTCCTCAGCGTCGGAACCAACGACCTGGGCCAGTACACGCTCGCCGCCGACAGACAGAGCGG
TCACCTCGCGGACCTGCTCAGCCCCTGGCAACCTGCGTTGCTCCGCCTCGTGGCGGACTGCGCGGCCGCCGGGGAAGCAT
CCGGCAAGCCAGTCGGGGTGTGCGGCGAGGCCGCGGCCGACCCCCTGCTCGCCGCCGTCCTGGTCGGGCTCGGAGTCACC
AGCCTGTCCATGTCAGGGCGGTCCATTGCGGCTGTCCGCGACTCCCTTGCCGCACACACGATCAAGGAGTGCCGGGCGCT
GGCAGAGATTGTGATCGACGCGGACGATGCGGAGCGCGCCCGCGAGCTGGCGACGAAGAACGCGCGGCAGACGTAA

Upstream 100 bases:

>100_bases
TCGAGCCCTGACCGGCGGGCCGCCGGCGACCACGATTTCAAACTGCCGGCTCCGGTTGCCGGCAGTCTGAGGCGATCCAG
TCTTCGAGGAGATGAAGAGC

Downstream 100 bases:

>100_bases
GGGGACCTCGGTCTCTCCCCGGCGGAACGGGTCTCGCGGCACCACCTGCACGCCCGGCCGCCGGTTCGTCGAGGGTCGAG
GCCGCGGCCGCGGCCGCGAG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MACRTATVNSRGGLHARPAALFVRAAAQQAVPVRIRKGDGPAVNAASMLSVLALGAMYGTVVTLEADGERAEEALDALAA
ILAHDQDAADSGQGDGRVGSASASDKALDETGGTGRSRATADVFVGLGVSPGLVTGPAFRMARQPRLPDPRLVLDPDEEA
AAAARVLRNVASDLRAHAAATRLSAASDIVTAQAMMAEDPVLLDEVGMRVRSGLDAVHAIDAALADQRRQLEGAGGYHAE
RAADIDDIRHRAVAALLGLPAPGLPAPGFPFVLVAEDLAPADTATLDTDLVLALVTERGGPTSHTAILARALGLPAVVSC
PGAMALDDGTPVRVDGTTGEVHVGVGVDVEADGAGHGEAIAQDTADVRQPGLWRTTSRAAKAGPARGGPGRTADGRPVQL
LLNIGSAKDLRGDVAATAEGVGLFRTEFLFLNRRVAPTPDEQRDAYQAVFRAAGSRKVVVRTLDAGADKPLPFLSLPDEP
NPALGVRGYRTVWLRPEVLDTQLGAIADAAAACDADVWVMAPMVSTPPEAEAFAAAARGHGLATTGVMVEVPAAALRAGR
MLDTVDFLSVGTNDLGQYTLAADRQSGHLADLLSPWQPALLRLVADCAAAGEASGKPVGVCGEAAADPLLAAVLVGLGVT
SLSMSGRSIAAVRDSLAAHTIKECRALAEIVIDADDAERARELATKNARQT

Sequences:

>Translated_691_residues
MACRTATVNSRGGLHARPAALFVRAAAQQAVPVRIRKGDGPAVNAASMLSVLALGAMYGTVVTLEADGERAEEALDALAA
ILAHDQDAADSGQGDGRVGSASASDKALDETGGTGRSRATADVFVGLGVSPGLVTGPAFRMARQPRLPDPRLVLDPDEEA
AAAARVLRNVASDLRAHAAATRLSAASDIVTAQAMMAEDPVLLDEVGMRVRSGLDAVHAIDAALADQRRQLEGAGGYHAE
RAADIDDIRHRAVAALLGLPAPGLPAPGFPFVLVAEDLAPADTATLDTDLVLALVTERGGPTSHTAILARALGLPAVVSC
PGAMALDDGTPVRVDGTTGEVHVGVGVDVEADGAGHGEAIAQDTADVRQPGLWRTTSRAAKAGPARGGPGRTADGRPVQL
LLNIGSAKDLRGDVAATAEGVGLFRTEFLFLNRRVAPTPDEQRDAYQAVFRAAGSRKVVVRTLDAGADKPLPFLSLPDEP
NPALGVRGYRTVWLRPEVLDTQLGAIADAAAACDADVWVMAPMVSTPPEAEAFAAAARGHGLATTGVMVEVPAAALRAGR
MLDTVDFLSVGTNDLGQYTLAADRQSGHLADLLSPWQPALLRLVADCAAAGEASGKPVGVCGEAAADPLLAAVLVGLGVT
SLSMSGRSIAAVRDSLAAHTIKECRALAEIVIDADDAERARELATKNARQT
>Mature_690_residues
ACRTATVNSRGGLHARPAALFVRAAAQQAVPVRIRKGDGPAVNAASMLSVLALGAMYGTVVTLEADGERAEEALDALAAI
LAHDQDAADSGQGDGRVGSASASDKALDETGGTGRSRATADVFVGLGVSPGLVTGPAFRMARQPRLPDPRLVLDPDEEAA
AAARVLRNVASDLRAHAAATRLSAASDIVTAQAMMAEDPVLLDEVGMRVRSGLDAVHAIDAALADQRRQLEGAGGYHAER
AADIDDIRHRAVAALLGLPAPGLPAPGFPFVLVAEDLAPADTATLDTDLVLALVTERGGPTSHTAILARALGLPAVVSCP
GAMALDDGTPVRVDGTTGEVHVGVGVDVEADGAGHGEAIAQDTADVRQPGLWRTTSRAAKAGPARGGPGRTADGRPVQLL
LNIGSAKDLRGDVAATAEGVGLFRTEFLFLNRRVAPTPDEQRDAYQAVFRAAGSRKVVVRTLDAGADKPLPFLSLPDEPN
PALGVRGYRTVWLRPEVLDTQLGAIADAAAACDADVWVMAPMVSTPPEAEAFAAAARGHGLATTGVMVEVPAAALRAGRM
LDTVDFLSVGTNDLGQYTLAADRQSGHLADLLSPWQPALLRLVADCAAAGEASGKPVGVCGEAAADPLLAAVLVGLGVTS
LSMSGRSIAAVRDSLAAHTIKECRALAEIVIDADDAERARELATKNARQT

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=448, Percent_Identity=38.3928571428571, Blast_Score=275, Evalue=9e-75,
Organism=Escherichia coli, GI1788726, Length=705, Percent_Identity=29.7872340425532, Blast_Score=232, Evalue=8e-62,
Organism=Escherichia coli, GI48994992, Length=523, Percent_Identity=32.1223709369025, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1789193, Length=548, Percent_Identity=30.2919708029197, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1787994, Length=417, Percent_Identity=28.2973621103118, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 70801; Mature: 70669

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00369 PTS_HPR_HIS ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACRTATVNSRGGLHARPAALFVRAAAQQAVPVRIRKGDGPAVNAASMLSVLALGAMYGT
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCE
VVTLEADGERAEEALDALAAILAHDQDAADSGQGDGRVGSASASDKALDETGGTGRSRAT
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCE
ADVFVGLGVSPGLVTGPAFRMARQPRLPDPRLVLDPDEEAAAAARVLRNVASDLRAHAAA
EEEEEECCCCCCCCCCHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
TRLSAASDIVTAQAMMAEDPVLLDEVGMRVRSGLDAVHAIDAALADQRRQLEGAGGYHAE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHH
RAADIDDIRHRAVAALLGLPAPGLPAPGFPFVLVAEDLAPADTATLDTDLVLALVTERGG
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHEEEEEECCCC
PTSHTAILARALGLPAVVSCPGAMALDDGTPVRVDGTTGEVHVGVGVDVEADGAGHGEAI
CCHHHHHHHHHHCCCCEEECCCCEEECCCCEEEEECCCCEEEEEECEEEECCCCCCCCHH
AQDTADVRQPGLWRTTSRAAKAGPARGGPGRTADGRPVQLLLNIGSAKDLRGDVAATAEG
HHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCC
VGLFRTEFLFLNRRVAPTPDEQRDAYQAVFRAAGSRKVVVRTLDAGADKPLPFLSLPDEP
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCC
NPALGVRGYRTVWLRPEVLDTQLGAIADAAAACDADVWVMAPMVSTPPEAEAFAAAARGH
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCC
GLATTGVMVEVPAAALRAGRMLDTVDFLSVGTNDLGQYTLAADRQSGHLADLLSPWQPAL
CCEECCEEEECCHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCHHHHH
LRLVADCAAAGEASGKPVGVCGEAAADPLLAAVLVGLGVTSLSMSGRSIAAVRDSLAAHT
HHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHH
IKECRALAEIVIDADDAERARELATKNARQT
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ACRTATVNSRGGLHARPAALFVRAAAQQAVPVRIRKGDGPAVNAASMLSVLALGAMYGT
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCE
VVTLEADGERAEEALDALAAILAHDQDAADSGQGDGRVGSASASDKALDETGGTGRSRAT
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCE
ADVFVGLGVSPGLVTGPAFRMARQPRLPDPRLVLDPDEEAAAAARVLRNVASDLRAHAAA
EEEEEECCCCCCCCCCHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
TRLSAASDIVTAQAMMAEDPVLLDEVGMRVRSGLDAVHAIDAALADQRRQLEGAGGYHAE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHH
RAADIDDIRHRAVAALLGLPAPGLPAPGFPFVLVAEDLAPADTATLDTDLVLALVTERGG
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHEEEEEECCCC
PTSHTAILARALGLPAVVSCPGAMALDDGTPVRVDGTTGEVHVGVGVDVEADGAGHGEAI
CCHHHHHHHHHHCCCCEEECCCCEEECCCCEEEEECCCCEEEEEECEEEECCCCCCCCHH
AQDTADVRQPGLWRTTSRAAKAGPARGGPGRTADGRPVQLLLNIGSAKDLRGDVAATAEG
HHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCC
VGLFRTEFLFLNRRVAPTPDEQRDAYQAVFRAAGSRKVVVRTLDAGADKPLPFLSLPDEP
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCC
NPALGVRGYRTVWLRPEVLDTQLGAIADAAAACDADVWVMAPMVSTPPEAEAFAAAARGH
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCC
GLATTGVMVEVPAAALRAGRMLDTVDFLSVGTNDLGQYTLAADRQSGHLADLLSPWQPAL
CCEECCEEEECCHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCHHHHH
LRLVADCAAAGEASGKPVGVCGEAAADPLLAAVLVGLGVTSLSMSGRSIAAVRDSLAAHT
HHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHH
IKECRALAEIVIDADDAERARELATKNARQT
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]