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The map label for this gene is purR [H]

Identifier: 158318774

GI number: 158318774

Start: 8595572

End: 8596594

Strand: Reverse

Name: purR [H]

Synonym: Franean1_7045

Alternate gene names: 158318774

Gene position: 8596594-8595572 (Counterclockwise)

Preceding gene: 158318777

Following gene: 158318773

Centisome position: 95.71

GC content: 67.35

Gene sequence:

>1023_bases
ATGCCAACCGCTCGGCTTGCCAGGCCATCCCAGCGCGACGTGGCCGCGCTCGCAGGGGTGTCGAGAACGACTGTGTCCTT
CGTGATGAACGGGGTTGCCGACGCCGCCAAGATCTCGCCGGAGACACGCGCACGGGTGTGGGCAGCCGTCGAGGAGCTGG
GGTTCCGGCCGAACGAGATCGCCCGTAGCCTTCGTTCGAGCAGAAGCAACGTGCTCGGTCTGGTCACCAGTGAGATCGCC
ACCACGCCCCATGCGGTGGCAATCATCAAGGGTGCGCAGGAGACCGCCTTCGCGCACGACAAGACGCTTCTGATCGTTGA
CACCGACGGCAGGCGGGACCTGGACGGAGAAACCATCACGAAGATGAGCCGGTGGCAGGTCGAGGGTCTGATCCTTGCCA
CCGACCACCACCGGGAACTGGTACCGCCCGACGGAATCGGCCAGACACCGACCATCCTCGTCAACTGTTTCCCGGCCGAC
GGTTCGCTGACATCCATCGTCCCGGACGAGCGACAGGGCGGCTGTCTGGCCACCGAGACCCTGCTCGCGGCCGGGCACCG
ACGCATCGGGTTCATCAACGGCCCGGATCGCTTCCCCGCGTCGGTCGGCCGCATGCGGGGATACCGCGACGCTCACCGGG
CCGCGGGAGTCGACCCGGACGAGCGGCTGATCCGCGTCGGCGACTGGTGGCAGGAGTCCGGTTTCCGGCATGCCGCCGAC
CTTCTCGGTCTTTCCGAACCGCCCACGGCCCTGTTCTGCGCCAACGACTGGATGGCCATGGGGGCGTACGACGCGGTGAG
GGAACGTGGCCTGCGCATCCCCGGGGACGTGGCGATCATCGGATTCGACAACCGCGAGCAGATCGCCGCGCACATGCGAC
CCCGACTGAGCACCGTCGCACTGCCCTACCACGACATGGGCAGGATGGCGGTCGAAAGGCTCCTGGCCACGGAACAGACC
GGGAGCACGGACGTCGAACGGCTTCTGGTGCCCTGTCCCCTGGTGACCCGTGAGTCGGTCTGA

Upstream 100 bases:

>100_bases
ACTCTGAGAGCCGGATGCCTCATGGGCTGGTAACACGTGTTAGTGTGCCGGGTTGCCGGCACGTGTCAAGATGTTTGCAA
TTTTTATGTGAACGGAGAAG

Downstream 100 bases:

>100_bases
ACACCACCCCGTCCGAGGGATGCCCTGGGGTGAGTTTCCCCTCTGAGCGTGGAGAACATCACGGACAGAGGATGAGGCGA
TTGTCATGGAATCGCGTCGT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MPTARLARPSQRDVAALAGVSRTTVSFVMNGVADAAKISPETRARVWAAVEELGFRPNEIARSLRSSRSNVLGLVTSEIA
TTPHAVAIIKGAQETAFAHDKTLLIVDTDGRRDLDGETITKMSRWQVEGLILATDHHRELVPPDGIGQTPTILVNCFPAD
GSLTSIVPDERQGGCLATETLLAAGHRRIGFINGPDRFPASVGRMRGYRDAHRAAGVDPDERLIRVGDWWQESGFRHAAD
LLGLSEPPTALFCANDWMAMGAYDAVRERGLRIPGDVAIIGFDNREQIAAHMRPRLSTVALPYHDMGRMAVERLLATEQT
GSTDVERLLVPCPLVTRESV

Sequences:

>Translated_340_residues
MPTARLARPSQRDVAALAGVSRTTVSFVMNGVADAAKISPETRARVWAAVEELGFRPNEIARSLRSSRSNVLGLVTSEIA
TTPHAVAIIKGAQETAFAHDKTLLIVDTDGRRDLDGETITKMSRWQVEGLILATDHHRELVPPDGIGQTPTILVNCFPAD
GSLTSIVPDERQGGCLATETLLAAGHRRIGFINGPDRFPASVGRMRGYRDAHRAAGVDPDERLIRVGDWWQESGFRHAAD
LLGLSEPPTALFCANDWMAMGAYDAVRERGLRIPGDVAIIGFDNREQIAAHMRPRLSTVALPYHDMGRMAVERLLATEQT
GSTDVERLLVPCPLVTRESV
>Mature_339_residues
PTARLARPSQRDVAALAGVSRTTVSFVMNGVADAAKISPETRARVWAAVEELGFRPNEIARSLRSSRSNVLGLVTSEIAT
TPHAVAIIKGAQETAFAHDKTLLIVDTDGRRDLDGETITKMSRWQVEGLILATDHHRELVPPDGIGQTPTILVNCFPADG
SLTSIVPDERQGGCLATETLLAAGHRRIGFINGPDRFPASVGRMRGYRDAHRAAGVDPDERLIRVGDWWQESGFRHAADL
LGLSEPPTALFCANDWMAMGAYDAVRERGLRIPGDVAIIGFDNREQIAAHMRPRLSTVALPYHDMGRMAVERLLATEQTG
STDVERLLVPCPLVTRESV

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=312, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=31.2684365781711, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=32.5301204819277, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=31.4189189189189, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789068, Length=311, Percent_Identity=32.7974276527331, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=30.1204819277108, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788474, Length=313, Percent_Identity=30.9904153354633, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=26.813880126183, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=27.3584905660377, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=27.9874213836478, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1786268, Length=313, Percent_Identity=25.5591054313099, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787906, Length=360, Percent_Identity=26.1111111111111, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=23.5649546827795, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789456, Length=339, Percent_Identity=25.9587020648968, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37010; Mature: 36878

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTARLARPSQRDVAALAGVSRTTVSFVMNGVADAAKISPETRARVWAAVEELGFRPNEI
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHH
ARSLRSSRSNVLGLVTSEIATTPHAVAIIKGAQETAFAHDKTLLIVDTDGRRDLDGETIT
HHHHHHCCCCEEEEEHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEECCCCCCCCCHHHH
KMSRWQVEGLILATDHHRELVPPDGIGQTPTILVNCFPADGSLTSIVPDERQGGCLATET
HHHHEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHH
LLAAGHRRIGFINGPDRFPASVGRMRGYRDAHRAAGVDPDERLIRVGDWWQESGFRHAAD
HHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHCCCCHHHHEEEECCCHHHCCCHHHHH
LLGLSEPPTALFCANDWMAMGAYDAVRERGLRIPGDVAIIGFDNREQIAAHMRPRLSTVA
HCCCCCCCCEEEECCCCEECCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCEEEE
LPYHDMGRMAVERLLATEQTGSTDVERLLVPCPLVTRESV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PTARLARPSQRDVAALAGVSRTTVSFVMNGVADAAKISPETRARVWAAVEELGFRPNEI
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHH
ARSLRSSRSNVLGLVTSEIATTPHAVAIIKGAQETAFAHDKTLLIVDTDGRRDLDGETIT
HHHHHHCCCCEEEEEHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEECCCCCCCCCHHHH
KMSRWQVEGLILATDHHRELVPPDGIGQTPTILVNCFPADGSLTSIVPDERQGGCLATET
HHHHEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHH
LLAAGHRRIGFINGPDRFPASVGRMRGYRDAHRAAGVDPDERLIRVGDWWQESGFRHAAD
HHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHCCCCHHHHEEEECCCHHHCCCHHHHH
LLGLSEPPTALFCANDWMAMGAYDAVRERGLRIPGDVAIIGFDNREQIAAHMRPRLSTVA
HCCCCCCCCEEEECCCCEECCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCEEEE
LPYHDMGRMAVERLLATEQTGSTDVERLLVPCPLVTRESV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA