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The map label for this gene is rbsB [H]

Identifier: 158318755

GI number: 158318755

Start: 8569328

End: 8570305

Strand: Reverse

Name: rbsB [H]

Synonym: Franean1_7026

Alternate gene names: 158318755

Gene position: 8570305-8569328 (Counterclockwise)

Preceding gene: 158318760

Following gene: 158318754

Centisome position: 95.42

GC content: 63.91

Gene sequence:

>978_bases
GTGCTATCTGCCTGTGGCAGTTCGTCGGACGGCAGCGCGAGCGGCGGGACGGACCAGGTGGTCGTTGGCCTGATCACCAA
GACCGACACCAACCCGTTCTTCGTCAAGATGAAGGAAGGCGCGGAGAAGGCGGCTGCGGCCAAGGGCGTCAAGCTGCTCA
CTGCGGCCGGCAAGTTTGACGGCGACAACGCCAGCCAGGTGACCGCCATCGAGAACATGGTTGCCTCGGGGGCCCGGGGC
ATCCTGATCACGCCTAGCGACACGAAGGCGATCATCCCCGCCATCGCGAAGGCACGGGCGAAGGGCGTGCTTGTCATCGC
GCTCGACAGTCCCACGGATCCGCAGAGCGCGGTGGATGCGCTGTTCGCGACGGACAACTTCCAGGCCGGGGTCCTGATCG
GCGAATACGCCAAGGGCATCACCGGCGGCGAGCCGGTGAAGATCGCGATGCTTGACCTCGCGCCCGGCATCACGGTTGGC
ACATTGCGGCACAACGGCTTCCTATCCGGTTTTGGTGCGGTAGACGTGGCTGACACCGCGAGCGAGCCAGTAAGCGCGGC
GAGCGTGGTGTGTAGCCAGGACACCCAGGGTGACCAGGCCAAGGGTCAGGCAGCTATGGAGACCTGCTTGCAGAAGGACC
CGGGCATCAACCTCGTCTACACGATCAACGAACCAGCTGCGCTCGGCGCTTACACTGCGATAGCTGCCGCCGGCAAGGAG
AATGACGTCACAATCGTCTCCGTCGACGGGGGATGCACAGGCGTCCAGGCAGTGAAGGACGGCAAGATTGCCGCGACGTC
GCAGCAGTACCCGCTGAAGATGGCCCAGCAGAGCGTGGACGCAGTCATTGCCTACGCCACCGACGGCACAAAGCCGTCCG
GGTACGTGGACACCGGTGTCACCTTGATCACCGCCAAGCCGGTGGACGGTATTGATTCGGAGAACGTCCAGTTCGGTCTC
GATAACTGCTGGGGTTGA

Upstream 100 bases:

>100_bases
GACCGTCACTGATGTGGCCCGGCGGCCGCGTCGTCGCCCGGTGCGTGGTTGGTGGCGGCGGTGCGCATCCCTCTTTGTGC
TGCCCGCTGCGGCAGCACTC

Downstream 100 bases:

>100_bases
TGGCTCCGAACGCCTCGGCATGACGCGCGGAGGCCGCGTACAGCCTCATCAGCGTGCCCGGCGCAAGTTGGCACGCTAAC
TGGACGGTTCGGCCGGGCGG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MLSACGSSSDGSASGGTDQVVVGLITKTDTNPFFVKMKEGAEKAAAAKGVKLLTAAGKFDGDNASQVTAIENMVASGARG
ILITPSDTKAIIPAIAKARAKGVLVIALDSPTDPQSAVDALFATDNFQAGVLIGEYAKGITGGEPVKIAMLDLAPGITVG
TLRHNGFLSGFGAVDVADTASEPVSAASVVCSQDTQGDQAKGQAAMETCLQKDPGINLVYTINEPAALGAYTAIAAAGKE
NDVTIVSVDGGCTGVQAVKDGKIAATSQQYPLKMAQQSVDAVIAYATDGTKPSGYVDTGVTLITAKPVDGIDSENVQFGL
DNCWG

Sequences:

>Translated_325_residues
MLSACGSSSDGSASGGTDQVVVGLITKTDTNPFFVKMKEGAEKAAAAKGVKLLTAAGKFDGDNASQVTAIENMVASGARG
ILITPSDTKAIIPAIAKARAKGVLVIALDSPTDPQSAVDALFATDNFQAGVLIGEYAKGITGGEPVKIAMLDLAPGITVG
TLRHNGFLSGFGAVDVADTASEPVSAASVVCSQDTQGDQAKGQAAMETCLQKDPGINLVYTINEPAALGAYTAIAAAGKE
NDVTIVSVDGGCTGVQAVKDGKIAATSQQYPLKMAQQSVDAVIAYATDGTKPSGYVDTGVTLITAKPVDGIDSENVQFGL
DNCWG
>Mature_325_residues
MLSACGSSSDGSASGGTDQVVVGLITKTDTNPFFVKMKEGAEKAAAAKGVKLLTAAGKFDGDNASQVTAIENMVASGARG
ILITPSDTKAIIPAIAKARAKGVLVIALDSPTDPQSAVDALFATDNFQAGVLIGEYAKGITGGEPVKIAMLDLAPGITVG
TLRHNGFLSGFGAVDVADTASEPVSAASVVCSQDTQGDQAKGQAAMETCLQKDPGINLVYTINEPAALGAYTAIAAAGKE
NDVTIVSVDGGCTGVQAVKDGKIAATSQQYPLKMAQQSVDAVIAYATDGTKPSGYVDTGVTLITAKPVDGIDSENVQFGL
DNCWG

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=262, Percent_Identity=31.6793893129771, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790526, Length=254, Percent_Identity=29.1338582677165, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790674, Length=271, Percent_Identity=27.6752767527675, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32765; Mature: 32765

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSACGSSSDGSASGGTDQVVVGLITKTDTNPFFVKMKEGAEKAAAAKGVKLLTAAGKFD
CCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHCCEEEEEECCCCC
GDNASQVTAIENMVASGARGILITPSDTKAIIPAIAKARAKGVLVIALDSPTDPQSAVDA
CCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHH
LFATDNFQAGVLIGEYAKGITGGEPVKIAMLDLAPGITVGTLRHNGFLSGFGAVDVADTA
HEECCCCCCCEEEEHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCEECCCCCEEECCCC
SEPVSAASVVCSQDTQGDQAKGQAAMETCLQKDPGINLVYTINEPAALGAYTAIAAAGKE
CCCCCHHEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHEECCCC
NDVTIVSVDGGCTGVQAVKDGKIAATSQQYPLKMAQQSVDAVIAYATDGTKPSGYVDTGV
CCEEEEEECCCCCCCEEECCCCEEECCCCCCHHHHHHCCCEEEEEECCCCCCCCEEECCE
TLITAKPVDGIDSENVQFGLDNCWG
EEEEECCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MLSACGSSSDGSASGGTDQVVVGLITKTDTNPFFVKMKEGAEKAAAAKGVKLLTAAGKFD
CCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHCCEEEEEECCCCC
GDNASQVTAIENMVASGARGILITPSDTKAIIPAIAKARAKGVLVIALDSPTDPQSAVDA
CCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHH
LFATDNFQAGVLIGEYAKGITGGEPVKIAMLDLAPGITVGTLRHNGFLSGFGAVDVADTA
HEECCCCCCCEEEEHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCEECCCCCEEECCCC
SEPVSAASVVCSQDTQGDQAKGQAAMETCLQKDPGINLVYTINEPAALGAYTAIAAAGKE
CCCCCHHEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHEECCCC
NDVTIVSVDGGCTGVQAVKDGKIAATSQQYPLKMAQQSVDAVIAYATDGTKPSGYVDTGV
CCEEEEEECCCCCCCEEECCCCEEECCCCCCHHHHHHCCCEEEEEECCCCCCCCEEECCE
TLITAKPVDGIDSENVQFGLDNCWG
EEEEECCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]