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The map label for this gene is pur8 [H]

Identifier: 158318676

GI number: 158318676

Start: 8461147

End: 8462643

Strand: Direct

Name: pur8 [H]

Synonym: Franean1_6944

Alternate gene names: 158318676

Gene position: 8461147-8462643 (Clockwise)

Preceding gene: 158318672

Following gene: 158318678

Centisome position: 94.2

GC content: 70.34

Gene sequence:

>1497_bases
ATGACCTTCACCGACTCCCGCACAACACACCAGGAGCCCGCGGCCACCGACGGGATCAACCCTGATCATCCTCGACGCTG
GTGGATCCTCGCTGTTCTCGGGGTCGCGCAGCTGATGGTCATCCTCGACACGACGATCGTCAACATCGCGATGCCCTCGG
CCCAGCGCGACCTGGGTTTCTCTGACGGTGCGAGGCAGTGGGTCATAACCGCCTACGCGCTGGCGTTCGGCAGTCTGCTG
CTGCTCGGCGGGCGCATCGCCGACCTGTTCGGCCGCAGGATGGCGCTGCTGGCCGGGTTGGCCGGCTTCGCCGCGGCCTC
CGCCGTCGGCGGGGCGGCCGTCAATTTCGGCATGCTGGTCGCCGCCCGTGCCAGCCAGGGCATGTTCGGCGCGCTGCTGG
CGCCCGCCGGCCTGTCCCTGTTGTCGACGACCTTCACCGAAACGCGGGAACGCGCCCGGGCGTTCGGCATCTACGGTGCG
ATCGCCGGCGCGGGCGGTGCGGTCGGCCTGCTGCTCGGTGGAGTCCTCACCGAATACCTCGACTGGCGCTGGTGCCTGTA
CGTCAACCTGGTCTTCGCCGCGGTGGCGATAGCCGGTGCGGCCGGCCTGATTCGCCGCCAGACCGTCGACGGACATCCGG
CCCTCGACCTGCCCGGTACTCTTGCTGTCTGCGCCGGCCTGTTCAGCCTGGTCTACGGCTTCGCCAACGCGCAGACCCAT
TCCTGGTCCGCTCCGGGCGCCTGGGCCCTGCTCCTCACCGGAGCCGCACTGCTGGCGCTGTTCGTGGCCCTGGAGACAAA
GGTCGCGCATCCCCTGCTCCCGATGCGGGTCCTGCTCGACCGCAATCGCGCCGGCGCCTACCTGACGGTCTTCGTCGTCG
GCGTCGGCGTGTTCGGGATCTTTCTCTTCCTGACCTACTACCTCCAGCAGAACCTCGGATTCTCGCCCATCCGTACCGGG
CTGGCGTTCCTCCCGATCGTCGGCGCGGTGATGATCACCGCCACCGTGGCGACCAGTGTCCTGTTTCCCCGGGTCGGTCC
ACGGCCGCTGATAGGCCTCGGCATGCTCACCGCCGCGGGCGGCATGCTGTGGCTCACCACGCTCGAGGTCCACAGCACCT
ACCTGGCCGACATCCTGCCCGCCCTCGTCATCGCCGGAGTCGGACTGGGGCTGGCGATGGCGCCGGCCATGAGCACCGCC
ACGGCGGGTGTCGAGCCCGACGACGCCGGGGTGGCCTCGGCGACCGTCAACACCGCGCAGCAGATCGGCGGGTCCATCGG
CATCGCCGTGCTCAGCACCATCGCCGCCACCGCCGCCAGTGACTACGCCCGCGGCAGGGCACCCGGCGCCGACCTGCTCG
CCCGTGCCGCCGTCCACAGCTACACCACCGCCTTCGCCTGGTCGGCAGCGATCTTCGCCGCGGGTGCCGTCCTCTGTGGG
CTGCTCGTCCGGCCCGGCGTCCCGGACACCACCGAGGTCACCAGCGCCGGGCTGTGA

Upstream 100 bases:

>100_bases
GGCCCATTCCCACACCGGGGCCCAGCCAGGTCCCGGCCCATCTCACCCCGAGAGTCAGCAGGGTTCCGAACAGGCACGAC
ACATGGACAGGGCGGTCGCC

Downstream 100 bases:

>100_bases
CTTGCCGGCACCGGATCGCCGGGCCGGGCCGGCGCCGGTCGTGGTCGCGTCAGGCGGGCGACGGCTGCCGCGCCCGCCTG
ACGCGACCGGGGAGCGCTGT

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTFTDSRTTHQEPAATDGINPDHPRRWWILAVLGVAQLMVILDTTIVNIAMPSAQRDLGFSDGARQWVITAYALAFGSLL
LLGGRIADLFGRRMALLAGLAGFAAASAVGGAAVNFGMLVAARASQGMFGALLAPAGLSLLSTTFTETRERARAFGIYGA
IAGAGGAVGLLLGGVLTEYLDWRWCLYVNLVFAAVAIAGAAGLIRRQTVDGHPALDLPGTLAVCAGLFSLVYGFANAQTH
SWSAPGAWALLLTGAALLALFVALETKVAHPLLPMRVLLDRNRAGAYLTVFVVGVGVFGIFLFLTYYLQQNLGFSPIRTG
LAFLPIVGAVMITATVATSVLFPRVGPRPLIGLGMLTAAGGMLWLTTLEVHSTYLADILPALVIAGVGLGLAMAPAMSTA
TAGVEPDDAGVASATVNTAQQIGGSIGIAVLSTIAATAASDYARGRAPGADLLARAAVHSYTTAFAWSAAIFAAGAVLCG
LLVRPGVPDTTEVTSAGL

Sequences:

>Translated_498_residues
MTFTDSRTTHQEPAATDGINPDHPRRWWILAVLGVAQLMVILDTTIVNIAMPSAQRDLGFSDGARQWVITAYALAFGSLL
LLGGRIADLFGRRMALLAGLAGFAAASAVGGAAVNFGMLVAARASQGMFGALLAPAGLSLLSTTFTETRERARAFGIYGA
IAGAGGAVGLLLGGVLTEYLDWRWCLYVNLVFAAVAIAGAAGLIRRQTVDGHPALDLPGTLAVCAGLFSLVYGFANAQTH
SWSAPGAWALLLTGAALLALFVALETKVAHPLLPMRVLLDRNRAGAYLTVFVVGVGVFGIFLFLTYYLQQNLGFSPIRTG
LAFLPIVGAVMITATVATSVLFPRVGPRPLIGLGMLTAAGGMLWLTTLEVHSTYLADILPALVIAGVGLGLAMAPAMSTA
TAGVEPDDAGVASATVNTAQQIGGSIGIAVLSTIAATAASDYARGRAPGADLLARAAVHSYTTAFAWSAAIFAAGAVLCG
LLVRPGVPDTTEVTSAGL
>Mature_497_residues
TFTDSRTTHQEPAATDGINPDHPRRWWILAVLGVAQLMVILDTTIVNIAMPSAQRDLGFSDGARQWVITAYALAFGSLLL
LGGRIADLFGRRMALLAGLAGFAAASAVGGAAVNFGMLVAARASQGMFGALLAPAGLSLLSTTFTETRERARAFGIYGAI
AGAGGAVGLLLGGVLTEYLDWRWCLYVNLVFAAVAIAGAAGLIRRQTVDGHPALDLPGTLAVCAGLFSLVYGFANAQTHS
WSAPGAWALLLTGAALLALFVALETKVAHPLLPMRVLLDRNRAGAYLTVFVVGVGVFGIFLFLTYYLQQNLGFSPIRTGL
AFLPIVGAVMITATVATSVLFPRVGPRPLIGLGMLTAAGGMLWLTTLEVHSTYLADILPALVIAGVGLGLAMAPAMSTAT
AGVEPDDAGVASATVNTAQQIGGSIGIAVLSTIAATAASDYARGRAPGADLLARAAVHSYTTAFAWSAAIFAAGAVLCGL
LVRPGVPDTTEVTSAGL

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=417, Percent_Identity=26.8585131894484, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789042, Length=412, Percent_Identity=24.5145631067961, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788710, Length=412, Percent_Identity=21.8446601941748, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=26.8408551068884, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788392, Length=417, Percent_Identity=26.8585131894484, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=385, Percent_Identity=25.1948051948052, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323520, Length=459, Percent_Identity=23.3115468409586, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323935, Length=419, Percent_Identity=26.0143198090692, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=476, Percent_Identity=22.4789915966387, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51108; Mature: 50977

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTDSRTTHQEPAATDGINPDHPRRWWILAVLGVAQLMVILDTTIVNIAMPSAQRDLGF
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
SDGARQWVITAYALAFGSLLLLGGRIADLFGRRMALLAGLAGFAAASAVGGAAVNFGMLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AARASQGMFGALLAPAGLSLLSTTFTETRERARAFGIYGAIAGAGGAVGLLLGGVLTEYL
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DWRWCLYVNLVFAAVAIAGAAGLIRRQTVDGHPALDLPGTLAVCAGLFSLVYGFANAQTH
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
SWSAPGAWALLLTGAALLALFVALETKVAHPLLPMRVLLDRNRAGAYLTVFVVGVGVFGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
FLFLTYYLQQNLGFSPIRTGLAFLPIVGAVMITATVATSVLFPRVGPRPLIGLGMLTAAG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
GMLWLTTLEVHSTYLADILPALVIAGVGLGLAMAPAMSTATAGVEPDDAGVASATVNTAQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QIGGSIGIAVLSTIAATAASDYARGRAPGADLLARAAVHSYTTAFAWSAAIFAAGAVLCG
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVRPGVPDTTEVTSAGL
HHHCCCCCCCCCHHCCCC
>Mature Secondary Structure 
TFTDSRTTHQEPAATDGINPDHPRRWWILAVLGVAQLMVILDTTIVNIAMPSAQRDLGF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC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HHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]