Click here to switch to the map view.

The map label for this gene is amiA2 [H]

Identifier: 158318632

GI number: 158318632

Start: 8405736

End: 8407190

Strand: Direct

Name: amiA2 [H]

Synonym: Franean1_6900

Alternate gene names: 158318632

Gene position: 8405736-8407190 (Clockwise)

Preceding gene: 158318629

Following gene: 158318633

Centisome position: 93.58

GC content: 75.74

Gene sequence:

>1455_bases
ATGTCGGATACCGCGGGTGTGGGTGCGGACGACGGCCGGCTCGACGGCCTGTACGGCCTGCGTGAGCTCGCCGGGCGACT
CGCGGCCGGGTCGATGAGCTCCCGGGAGCTCGTCGCCGCCTGCCTGGAGCGCATCGAGGCGACGTCCACGACCCTGAACA
CCTTCCGGGTGCTACGGGCCGAGGAGGCGCTGGCGGAGGCCGAGGAGGCTGACCGGCGGCTCGACGCCGGGGAGCGACTG
CCCCTGCTGGGTGTCCCGGTGGCCATCAAGGACGACACCGACATGGCCGGCCTCCCCACCGCCTTCGGCGCGGCCGGCGA
CTTCCCGCCGGCCACCGAGGACTGCGAGATGGTCCGGCGGCTGCGGGCCACCGGCGCGATCATCGTCGGCAAGACGAACA
CGCCCGAGTTCGGGCAGTGGCCGTTCACCGAGGGGGCGGCGTTCGGCGCGACCCGCAACCCGTGGAACCTCGACTACTCC
CCCGGTGGGTCGTCCGGCGGCGCGGCGTCGGCCGTCGCGGCCGGGCTGGTCCCCGCCGCGATCGGGTCGGACGGCGCCGG
CTCGGTCCGCATCCCCGCGGCCTGGACCCATCTCGTCGGGATCAAGCCGCAGCGCGGGCGCATCTCCAGCGCGCCGGTAA
CCGAGCAGTTCAACGGGCTGACCACCTTCGGCCCGCTCACCAGGACCGTCGCGGACGGCGCCTTCCTGCTGGACCTGCTC
AGCGGAAGCCTCCCGGTGGACCGGGACCGCCCGCCGGCGCCGACGGAGACCTTCCTGCGCTCGGCCGAGCACCCGCCGCC
CCGGCTGCGGATCGGCCTGTCGCTGCGCGTCCCCTACAGCGGGATGTCGGCCGCGCTGGATCCGGCCGTCCGCGCCGCCG
TGGAGCGCCTCGCCGAGGTGCTGGCCCGGCTCGGGCACGACGTGGTGCCGGTCGAGCCGCCGTACGGCCTGCTCGGCCTG
CTGTTCCTGCCCCGCTCGATGGACGGCATCCGGGACTGGTCGCACCGGGTGCCCAACCCCGAGCTCCTCGATCCGCGGAC
GGTGGCGAACGCGCGCAACGGGCGGGCGCTGCGGCCGGTGCTCGGGCTGGCCCGGGCTGCCGAGGGGCCGGCCCGGGCGT
GGATCGGCCGGGTCTTCCGCACGGTGGACGTCCTGCTCGCGCCGACCACCGCGGTGCCGCCGCCGCGGGTCGGCGAACTG
AACCGGGAGACGAACTGGGAGACCGATCAGGCGATCGTCGCGGCCTGCCCGTACACCTGGCCGTGGAACGTCCTCGGCTG
GCCGGCGGTCAACGTCCCGGCCGGGCTGACGCCTGCCGGGCTGCCGGTCGGCGCCCAGCTCATGGGCCCGGCGGGCGGCG
AGGGGCCGTTGATCTCCCTGGCCGCCGAGCTGGAGGACGTCGAGCGTTGGTACAGGCGCCGGCCGGGCCGCCTGGAGGCT
TCGACGCCTGCCTGA

Upstream 100 bases:

>100_bases
TGTACGTCTCGTGCTGGACAAAGGGCGGGGGCCGCGGCGCGCGGTCGGCTACCGTTCCCCCACGGCTTCCGCCGCGCGCG
TCGCGTCCAGGAGGTGGCTG

Downstream 100 bases:

>100_bases
CCCGGAGGCGTCTGAGCTGGAGGCGCCTGCGGTCGGAGGCTAGCGACTGATCCGGATCGTCACCGCCGGATGCACACAGC
GGGACACCGGCACGTGAAGC

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSDTAGVGADDGRLDGLYGLRELAGRLAAGSMSSRELVAACLERIEATSTTLNTFRVLRAEEALAEAEEADRRLDAGERL
PLLGVPVAIKDDTDMAGLPTAFGAAGDFPPATEDCEMVRRLRATGAIIVGKTNTPEFGQWPFTEGAAFGATRNPWNLDYS
PGGSSGGAASAVAAGLVPAAIGSDGAGSVRIPAAWTHLVGIKPQRGRISSAPVTEQFNGLTTFGPLTRTVADGAFLLDLL
SGSLPVDRDRPPAPTETFLRSAEHPPPRLRIGLSLRVPYSGMSAALDPAVRAAVERLAEVLARLGHDVVPVEPPYGLLGL
LFLPRSMDGIRDWSHRVPNPELLDPRTVANARNGRALRPVLGLARAAEGPARAWIGRVFRTVDVLLAPTTAVPPPRVGEL
NRETNWETDQAIVAACPYTWPWNVLGWPAVNVPAGLTPAGLPVGAQLMGPAGGEGPLISLAAELEDVERWYRRRPGRLEA
STPA

Sequences:

>Translated_484_residues
MSDTAGVGADDGRLDGLYGLRELAGRLAAGSMSSRELVAACLERIEATSTTLNTFRVLRAEEALAEAEEADRRLDAGERL
PLLGVPVAIKDDTDMAGLPTAFGAAGDFPPATEDCEMVRRLRATGAIIVGKTNTPEFGQWPFTEGAAFGATRNPWNLDYS
PGGSSGGAASAVAAGLVPAAIGSDGAGSVRIPAAWTHLVGIKPQRGRISSAPVTEQFNGLTTFGPLTRTVADGAFLLDLL
SGSLPVDRDRPPAPTETFLRSAEHPPPRLRIGLSLRVPYSGMSAALDPAVRAAVERLAEVLARLGHDVVPVEPPYGLLGL
LFLPRSMDGIRDWSHRVPNPELLDPRTVANARNGRALRPVLGLARAAEGPARAWIGRVFRTVDVLLAPTTAVPPPRVGEL
NRETNWETDQAIVAACPYTWPWNVLGWPAVNVPAGLTPAGLPVGAQLMGPAGGEGPLISLAAELEDVERWYRRRPGRLEA
STPA
>Mature_483_residues
SDTAGVGADDGRLDGLYGLRELAGRLAAGSMSSRELVAACLERIEATSTTLNTFRVLRAEEALAEAEEADRRLDAGERLP
LLGVPVAIKDDTDMAGLPTAFGAAGDFPPATEDCEMVRRLRATGAIIVGKTNTPEFGQWPFTEGAAFGATRNPWNLDYSP
GGSSGGAASAVAAGLVPAAIGSDGAGSVRIPAAWTHLVGIKPQRGRISSAPVTEQFNGLTTFGPLTRTVADGAFLLDLLS
GSLPVDRDRPPAPTETFLRSAEHPPPRLRIGLSLRVPYSGMSAALDPAVRAAVERLAEVLARLGHDVVPVEPPYGLLGLL
FLPRSMDGIRDWSHRVPNPELLDPRTVANARNGRALRPVLGLARAAEGPARAWIGRVFRTVDVLLAPTTAVPPPRVGELN
RETNWETDQAIVAACPYTWPWNVLGWPAVNVPAGLTPAGLPVGAQLMGPAGGEGPLISLAAELEDVERWYRRRPGRLEAS
TPA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=515, Percent_Identity=28.7378640776699, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI195972892, Length=237, Percent_Identity=29.535864978903, Blast_Score=103, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=528, Percent_Identity=24.2424242424242, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=256, Percent_Identity=30.859375, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=317, Percent_Identity=30.2839116719243, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=317, Percent_Identity=30.2839116719243, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=311, Percent_Identity=30.2250803858521, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=25.0505050505051, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32565337, Length=314, Percent_Identity=28.343949044586, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=300, Percent_Identity=27, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=200, Percent_Identity=31, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=444, Percent_Identity=27.2522522522523, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=435, Percent_Identity=27.1264367816092, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21356731, Length=491, Percent_Identity=27.6985743380855, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24644968, Length=492, Percent_Identity=25.4065040650407, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24648113, Length=497, Percent_Identity=30.3822937625755, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078093, Length=488, Percent_Identity=27.4590163934426, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648435, Length=483, Percent_Identity=25.0517598343685, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648437, Length=483, Percent_Identity=25.0517598343685, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45550774, Length=487, Percent_Identity=25.4620123203285, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=25.609756097561, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=25.609756097561, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=25.609756097561, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=25.609756097561, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648441, Length=419, Percent_Identity=24.3436754176611, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648439, Length=419, Percent_Identity=24.3436754176611, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 51058; Mature: 50926

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTAGVGADDGRLDGLYGLRELAGRLAAGSMSSRELVAACLERIEATSTTLNTFRVLRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEALAEAEEADRRLDAGERLPLLGVPVAIKDDTDMAGLPTAFGAAGDFPPATEDCEMVRR
HHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHH
LRATGAIIVGKTNTPEFGQWPFTEGAAFGATRNPWNLDYSPGGSSGGAASAVAAGLVPAA
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IGSDGAGSVRIPAAWTHLVGIKPQRGRISSAPVTEQFNGLTTFGPLTRTVADGAFLLDLL
CCCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHH
SGSLPVDRDRPPAPTETFLRSAEHPPPRLRIGLSLRVPYSGMSAALDPAVRAAVERLAEV
CCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LARLGHDVVPVEPPYGLLGLLFLPRSMDGIRDWSHRVPNPELLDPRTVANARNGRALRPV
HHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHH
LGLARAAEGPARAWIGRVFRTVDVLLAPTTAVPPPRVGELNRETNWETDQAIVAACPYTW
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCC
PWNVLGWPAVNVPAGLTPAGLPVGAQLMGPAGGEGPLISLAAELEDVERWYRRRPGRLEA
CCCCCCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCC
STPA
CCCC
>Mature Secondary Structure 
SDTAGVGADDGRLDGLYGLRELAGRLAAGSMSSRELVAACLERIEATSTTLNTFRVLRA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEALAEAEEADRRLDAGERLPLLGVPVAIKDDTDMAGLPTAFGAAGDFPPATEDCEMVRR
HHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHH
LRATGAIIVGKTNTPEFGQWPFTEGAAFGATRNPWNLDYSPGGSSGGAASAVAAGLVPAA
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IGSDGAGSVRIPAAWTHLVGIKPQRGRISSAPVTEQFNGLTTFGPLTRTVADGAFLLDLL
CCCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHH
SGSLPVDRDRPPAPTETFLRSAEHPPPRLRIGLSLRVPYSGMSAALDPAVRAAVERLAEV
CCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LARLGHDVVPVEPPYGLLGLLFLPRSMDGIRDWSHRVPNPELLDPRTVANARNGRALRPV
HHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHH
LGLARAAEGPARAWIGRVFRTVDVLLAPTTAVPPPRVGELNRETNWETDQAIVAACPYTW
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCC
PWNVLGWPAVNVPAGLTPAGLPVGAQLMGPAGGEGPLISLAAELEDVERWYRRRPGRLEA
CCCCCCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCC
STPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]