Click here to switch to the map view.

The map label for this gene is nifS [H]

Identifier: 158318600

GI number: 158318600

Start: 8373099

End: 8374319

Strand: Reverse

Name: nifS [H]

Synonym: Franean1_6868

Alternate gene names: 158318600

Gene position: 8374319-8373099 (Counterclockwise)

Preceding gene: 158318601

Following gene: 158318599

Centisome position: 93.23

GC content: 74.45

Gene sequence:

>1221_bases
GTGATCTACCTCGACCACAACGCGACCACGCCCGTCGACCCGCGGGTGGTCGAGGTGATGCTCCCGCTGTTCACCGAGCG
GTTCGCCAACCCGGCCAGCAAGCACGCCCCCGGCCAGGAGGCGACGCGGATCGTCGAGCGGGCCCGGCGCGAGGTCGCGT
CGCTCGCCGGCGCCCGCCCCCGCGAGGTCGTGTTCACCTCCGGGGCGACCGAGGCCGCCAACCTGGCGATCGGCGGGGTC
CTCGCCACCGCCGAGCCGGGGCGACGCCGGATCCTCGTCGGGGCCACCGAGCATCCCGCGGTGCTCGGCGCCGCCGACGC
CGCCGCCGCAGCGGCCAACGCAACAGTCGCCGAGCGGATCGCCGTCCACCCGGACGGCACCGTCGACGTCGACGACCTGC
GGCGCCGGCTCGGGCCGGACGTCGCGCTGGTCGCGGTGATGGCGGCGAACAACGAGACCGGGGCCGTGAACGACCTGCGG
CCACTGGTCGAACCGGTGCACTCCGCCGGGGCGTTGCTCCTGTGCGACGTCACCCAGGCCCTCGGCCGCATCCCGGTCGA
GCTCGAGCAGACAGGCGTCGACCTGGCCGTCGCGTCCGCGCACAAGCTCTACGGCCCCAAAGGCGTCGGCGCGCTGATCG
CGTCGAAGGACGCCTGGCCGCGCCTCGCGCCCAGGGTGCACGGCGGCGGCCAGGAACGCGGCCTGCGGGCCGGCACCCTC
AACACCGCCGGGATCGCCGGCTTCGGAGCGGCCGCCCGCATCGCGGCCGAGTCGATGGCCGACGACGGCAGGCACCAGGG
CGAGCTGACGGCGCTGCTGACTGCGCTGTTGACGGAGAACCTGGCGAAGCGGCTCGGGCCCGGAGCCGTCGAGCTCAACG
GCCCGGACCGCGCCAGGCTGCCGAACACCGTCAATCTCCGCTTCGTCGGCGCCGGTGCCGACGAGGTCCAGGCCTGTGCT
CCGCGCGTGGCGATCTCGGCCGGCTCGGCGTGCAGCGGTGGCGGGGACGAGCCGTCCCACGTCCTGCTCGCCATGGGCCG
CACGGCCACCGAGGCCCGGGAGAGCCTGCGCTTCAGCCTGGGCCGCGCCACCACGACGGAGGAGATCCGCACCGCCGCGG
ATCTGGTGGCCCGCGCTGTCCTGCGCGTCCGCTCCCTTTCCCAGAGCACCCCCATCCAAGACGAGGTTCGAGATGACAAC
CGAGCTCTTCGAACAGCCTGA

Upstream 100 bases:

>100_bases
ACCTGGACTTCCAGGTGCTGCGGGCCCGGCGCGACCCGCACTGCCCGACCTGCGGCGGACCGTCCGGCAGCGGCCAGCGA
CCCGGCCCCGGGGAGCCACA

Downstream 100 bases:

>100_bases
ATCGCGGGTACGCCGCCTCAAGGGGGTCGTGATCCGGTTCGCCGGCGACTCCGGCGACGGCATGCAGCTCACCGGCGACC
GCTTCGGCGCGCAGGCCGCG

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MIYLDHNATTPVDPRVVEVMLPLFTERFANPASKHAPGQEATRIVERARREVASLAGARPREVVFTSGATEAANLAIGGV
LATAEPGRRRILVGATEHPAVLGAADAAAAAANATVAERIAVHPDGTVDVDDLRRRLGPDVALVAVMAANNETGAVNDLR
PLVEPVHSAGALLLCDVTQALGRIPVELEQTGVDLAVASAHKLYGPKGVGALIASKDAWPRLAPRVHGGGQERGLRAGTL
NTAGIAGFGAAARIAAESMADDGRHQGELTALLTALLTENLAKRLGPGAVELNGPDRARLPNTVNLRFVGAGADEVQACA
PRVAISAGSACSGGGDEPSHVLLAMGRTATEARESLRFSLGRATTTEEIRTAADLVARAVLRVRSLSQSTPIQDEVRDDN
RALRTA

Sequences:

>Translated_406_residues
MIYLDHNATTPVDPRVVEVMLPLFTERFANPASKHAPGQEATRIVERARREVASLAGARPREVVFTSGATEAANLAIGGV
LATAEPGRRRILVGATEHPAVLGAADAAAAAANATVAERIAVHPDGTVDVDDLRRRLGPDVALVAVMAANNETGAVNDLR
PLVEPVHSAGALLLCDVTQALGRIPVELEQTGVDLAVASAHKLYGPKGVGALIASKDAWPRLAPRVHGGGQERGLRAGTL
NTAGIAGFGAAARIAAESMADDGRHQGELTALLTALLTENLAKRLGPGAVELNGPDRARLPNTVNLRFVGAGADEVQACA
PRVAISAGSACSGGGDEPSHVLLAMGRTATEARESLRFSLGRATTTEEIRTAADLVARAVLRVRSLSQSTPIQDEVRDDN
RALRTA
>Mature_406_residues
MIYLDHNATTPVDPRVVEVMLPLFTERFANPASKHAPGQEATRIVERARREVASLAGARPREVVFTSGATEAANLAIGGV
LATAEPGRRRILVGATEHPAVLGAADAAAAAANATVAERIAVHPDGTVDVDDLRRRLGPDVALVAVMAANNETGAVNDLR
PLVEPVHSAGALLLCDVTQALGRIPVELEQTGVDLAVASAHKLYGPKGVGALIASKDAWPRLAPRVHGGGQERGLRAGTL
NTAGIAGFGAAARIAAESMADDGRHQGELTALLTALLTENLAKRLGPGAVELNGPDRARLPNTVNLRFVGAGADEVQACA
PRVAISAGSACSGGGDEPSHVLLAMGRTATEARESLRFSLGRATTTEEIRTAADLVARAVLRVRSLSQSTPIQDEVRDDN
RALRTA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=398, Percent_Identity=37.1859296482412, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=33.3333333333333, Blast_Score=184, Evalue=9e-47,
Organism=Escherichia coli, GI48994898, Length=394, Percent_Identity=40.3553299492386, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=29.5, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787970, Length=267, Percent_Identity=28.8389513108614, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=400, Percent_Identity=38.75, Blast_Score=260, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=32, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=37.0927318295739, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI20129463, Length=399, Percent_Identity=36.8421052631579, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42094; Mature: 42094

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDHNATTPVDPRVVEVMLPLFTERFANPASKHAPGQEATRIVERARREVASLAGARP
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
REVVFTSGATEAANLAIGGVLATAEPGRRRILVGATEHPAVLGAADAAAAAANATVAERI
CEEEEECCCCHHHHHEECEEEECCCCCCCEEEEECCCCCCEEECHHHHHHHHCCHHHHHE
AVHPDGTVDVDDLRRRLGPDVALVAVMAANNETGAVNDLRPLVEPVHSAGALLLCDVTQA
EECCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHH
LGRIPVELEQTGVDLAVASAHKLYGPKGVGALIASKDAWPRLAPRVHGGGQERGLRAGTL
HCCCCCEEHHCCCCEEEHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCHHCCCCCCCC
NTAGIAGFGAAARIAAESMADDGRHQGELTALLTALLTENLAKRLGPGAVELNGPDRARL
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
PNTVNLRFVGAGADEVQACAPRVAISAGSACSGGGDEPSHVLLAMGRTATEARESLRFSL
CCCEEEEEEECCHHHHHHHCCHHEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
GRATTTEEIRTAADLVARAVLRVRSLSQSTPIQDEVRDDNRALRTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHCCC
>Mature Secondary Structure
MIYLDHNATTPVDPRVVEVMLPLFTERFANPASKHAPGQEATRIVERARREVASLAGARP
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
REVVFTSGATEAANLAIGGVLATAEPGRRRILVGATEHPAVLGAADAAAAAANATVAERI
CEEEEECCCCHHHHHEECEEEECCCCCCCEEEEECCCCCCEEECHHHHHHHHCCHHHHHE
AVHPDGTVDVDDLRRRLGPDVALVAVMAANNETGAVNDLRPLVEPVHSAGALLLCDVTQA
EECCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHH
LGRIPVELEQTGVDLAVASAHKLYGPKGVGALIASKDAWPRLAPRVHGGGQERGLRAGTL
HCCCCCEEHHCCCCEEEHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCHHCCCCCCCC
NTAGIAGFGAAARIAAESMADDGRHQGELTALLTALLTENLAKRLGPGAVELNGPDRARL
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
PNTVNLRFVGAGADEVQACAPRVAISAGSACSGGGDEPSHVLLAMGRTATEARESLRFSL
CCCEEEEEEECCHHHHHHHCCHHEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
GRATTTEEIRTAADLVARAVLRVRSLSQSTPIQDEVRDDNRALRTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA