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The map label for this gene is ddl [H]

Identifier: 158318443

GI number: 158318443

Start: 8152574

End: 8153539

Strand: Direct

Name: ddl [H]

Synonym: Franean1_6711

Alternate gene names: 158318443

Gene position: 8152574-8153539 (Clockwise)

Preceding gene: 158318442

Following gene: 158318445

Centisome position: 90.77

GC content: 73.08

Gene sequence:

>966_bases
ATGACTGATCTGGGCCGTGTGCTGATCCTCGCCGGCGGGCTGTCCCCCGAACGGGAGGTCTCCCTGCATTCGGGGGGGAA
GCTGCGCGACGCACTGACCCGCATCGGGGTCGAGGCGGAGCTGGCCGACGTCGATGCCGCGACGCTGCCTGCGCTGGCGG
CCGACCCGCCCGACGTGGTGTTCCCGGTGCTGCACGGGACCTCGGGCGAGGACGGCACGATCCGCGAGGTGCTGGATCTG
TACGGCCTGCCCTACGTCGGTGCCCTGCCGCCGGCCTGCCGGGTGGCGTTCGACAAGGCGACAGCGAAGGCGGCGGTCGG
CGCCGCGGGGGTGGCGACACCGGAGGCGGTGACGCTGCCCCGCGAGGCGTTCCACGACCTGGGGGCCGCCGCGCTGATCG
AGCGGATCGTCGCCCGGCTGGGACTGCCGGTCGTGGTGAAGCCCCGCGCGGGTGGGTCGGCGTTCGGGGTGACGCACGTC
GACGACGCGGGTGGACTCGCCGAGGCGTTGATGAGCTGCTTCGGGTACCACGACTCGGCGCTGATCGAACAGGCCGTCGC
CGGGGTCGAGATCGCCATCGGTGTCGTCGACAGCGGCGACGGGCCGGTCGCGTTGCCGGCCGTCGAGATCGAGCCGAGGG
CGGGGGTGTACGACTACGCGGCCCGCTACACTGCGGGCGCCACCTCGTTCTTCACCCCGGCGCGCCTGCACAAGGACGTG
CTGTGGGCTGCGGCTGAGACCGCGCTCACGGCGCACCGGGTGCTGGGGCTGCGGGACCTGTCCCGCACCGACATGATCGT
CGACCGTGACGGTGTCGCGCAGTTCCTGGAGGTGAACGTGGCCCCGGGCATGACCGACACGAGCACCTGGCCGATGGGGC
TGCACGCCGCCGGTCTCGACTTCGCCGTGGTCTGCCGCGACCTCGCGGCCCACGCCTGCGACCGCCGCGACGTCGCTCGC
TGCTGA

Upstream 100 bases:

>100_bases
GGGGTCGGCCGGTTGGGCGCGGTCGTGCGGGATGAGCTGGCCCTGCGTGACACCTTCGGTTCCCGTCCCTGATGTGCCCG
ACGCTGTGAAGGATCCGACG

Downstream 100 bases:

>100_bases
GCGCGGGAGCCGCCGCTCAGCGGCGATACACCGAGACGTGCTTGCGGGCCGTGTTGGGGTCGTAGGCGGTGGCGTCCCAG
TCGCCCCACCGGTGCGCGAG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTDLGRVLILAGGLSPEREVSLHSGGKLRDALTRIGVEAELADVDAATLPALAADPPDVVFPVLHGTSGEDGTIREVLDL
YGLPYVGALPPACRVAFDKATAKAAVGAAGVATPEAVTLPREAFHDLGAAALIERIVARLGLPVVVKPRAGGSAFGVTHV
DDAGGLAEALMSCFGYHDSALIEQAVAGVEIAIGVVDSGDGPVALPAVEIEPRAGVYDYAARYTAGATSFFTPARLHKDV
LWAAAETALTAHRVLGLRDLSRTDMIVDRDGVAQFLEVNVAPGMTDTSTWPMGLHAAGLDFAVVCRDLAAHACDRRDVAR
C

Sequences:

>Translated_321_residues
MTDLGRVLILAGGLSPEREVSLHSGGKLRDALTRIGVEAELADVDAATLPALAADPPDVVFPVLHGTSGEDGTIREVLDL
YGLPYVGALPPACRVAFDKATAKAAVGAAGVATPEAVTLPREAFHDLGAAALIERIVARLGLPVVVKPRAGGSAFGVTHV
DDAGGLAEALMSCFGYHDSALIEQAVAGVEIAIGVVDSGDGPVALPAVEIEPRAGVYDYAARYTAGATSFFTPARLHKDV
LWAAAETALTAHRVLGLRDLSRTDMIVDRDGVAQFLEVNVAPGMTDTSTWPMGLHAAGLDFAVVCRDLAAHACDRRDVAR
C
>Mature_320_residues
TDLGRVLILAGGLSPEREVSLHSGGKLRDALTRIGVEAELADVDAATLPALAADPPDVVFPVLHGTSGEDGTIREVLDLY
GLPYVGALPPACRVAFDKATAKAAVGAAGVATPEAVTLPREAFHDLGAAALIERIVARLGLPVVVKPRAGGSAFGVTHVD
DAGGLAEALMSCFGYHDSALIEQAVAGVEIAIGVVDSGDGPVALPAVEIEPRAGVYDYAARYTAGATSFFTPARLHKDVL
WAAAETALTAHRVLGLRDLSRTDMIVDRDGVAQFLEVNVAPGMTDTSTWPMGLHAAGLDFAVVCRDLAAHACDRRDVARC

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=310, Percent_Identity=35.8064516129032, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786579, Length=355, Percent_Identity=27.6056338028169, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33230; Mature: 33099

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLGRVLILAGGLSPEREVSLHSGGKLRDALTRIGVEAELADVDAATLPALAADPPDVV
CCCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHCCCHHHHCCCHHHCCHHCCCCCCEE
FPVLHGTSGEDGTIREVLDLYGLPYVGALPPACRVAFDKATAKAAVGAAGVATPEAVTLP
EEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
REAFHDLGAAALIERIVARLGLPVVVKPRAGGSAFGVTHVDDAGGLAEALMSCFGYHDSA
HHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCCCHHHHHHHHHCCCHHH
LIEQAVAGVEIAIGVVDSGDGPVALPAVEIEPRAGVYDYAARYTAGATSFFTPARLHKDV
HHHHHHCCEEEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHCCCCCHHCCHHHHHHHH
LWAAAETALTAHRVLGLRDLSRTDMIVDRDGVAQFLEVNVAPGMTDTSTWPMGLHAAGLD
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHEEECCCCCCCCCCCCCCCCCCCCH
FAVVCRDLAAHACDRRDVARC
HHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
TDLGRVLILAGGLSPEREVSLHSGGKLRDALTRIGVEAELADVDAATLPALAADPPDVV
CCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHCCCHHHHCCCHHHCCHHCCCCCCEE
FPVLHGTSGEDGTIREVLDLYGLPYVGALPPACRVAFDKATAKAAVGAAGVATPEAVTLP
EEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
REAFHDLGAAALIERIVARLGLPVVVKPRAGGSAFGVTHVDDAGGLAEALMSCFGYHDSA
HHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCCCHHHHHHHHHCCCHHH
LIEQAVAGVEIAIGVVDSGDGPVALPAVEIEPRAGVYDYAARYTAGATSFFTPARLHKDV
HHHHHHCCEEEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHCCCCCHHCCHHHHHHHH
LWAAAETALTAHRVLGLRDLSRTDMIVDRDGVAQFLEVNVAPGMTDTSTWPMGLHAAGLD
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHEEECCCCCCCCCCCCCCCCCCCCH
FAVVCRDLAAHACDRRDVARC
HHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA