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The map label for this gene is mscL [H]

Identifier: 158318223

GI number: 158318223

Start: 7887912

End: 7888421

Strand: Direct

Name: mscL [H]

Synonym: Franean1_6488

Alternate gene names: 158318223

Gene position: 7887912-7888421 (Clockwise)

Preceding gene: 158318222

Following gene: 158318225

Centisome position: 87.82

GC content: 66.47

Gene sequence:

>510_bases
GTGTTCGGGTGGCGGAAGGTCGTCAGTAGTGGTCGCGGTGTCGTCGAGCCCCCGACGCGGCTGATCACTCCGGGGCTGCG
GGGCTTCAAGCAGTTCCTGATGCGGGGCAACATCGTCGACCTGGCCATCGCGGTGGTCGTCGGCACGGCGTTCACGGCCG
TCGTCCAGTCGCTGGTGAAGAACATCTTCACGCCGTTGATCGCCGCGATCGGCGGCGAGCCGGACTTCTCCAGCCTGACG
TTCACCATCAACGACAGCAAGTTCAGGTACGGGGCGTTCATCAACGATGTGATCGCGTTCATCGTTGTCGCGGCGGTGAT
CTATTTCGTGATCGTCCTGCCGCTCGCCAAGGCGAACGAGATGCGCCGGCGTGGCGAGGAACCGGAGCCGCAGGAGGAGC
CGGCGATCAGCGACGAGGCGAAGCTGCTCACCGAGATCCGCGACCTGCTCGCGGCGAACAACAACCCCGCCGCCGCGCCG
CTGGCCGGCCAGCCGCCGCAGTACGGCTGA

Upstream 100 bases:

>100_bases
ACCCGGATCTCACGCATCCGATGACCTGGGGTTTTACTCCCGATCATCCGGCTATAGCCTCCCCTCGGCAGTTTTGGTCC
GAGGTTGTGGAGGAACAGGC

Downstream 100 bases:

>100_bases
CGGACCAGCGACCGGACGGCCGACCCGCCAGGCTGGGCTCGCCGTGGGCCCAGCCCGGCGGCCTCGAACCCGGGGCTCCA
GTCGCCGGTCACTCGAACCC

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MFGWRKVVSSGRGVVEPPTRLITPGLRGFKQFLMRGNIVDLAIAVVVGTAFTAVVQSLVKNIFTPLIAAIGGEPDFSSLT
FTINDSKFRYGAFINDVIAFIVVAAVIYFVIVLPLAKANEMRRRGEEPEPQEEPAISDEAKLLTEIRDLLAANNNPAAAP
LAGQPPQYG

Sequences:

>Translated_169_residues
MFGWRKVVSSGRGVVEPPTRLITPGLRGFKQFLMRGNIVDLAIAVVVGTAFTAVVQSLVKNIFTPLIAAIGGEPDFSSLT
FTINDSKFRYGAFINDVIAFIVVAAVIYFVIVLPLAKANEMRRRGEEPEPQEEPAISDEAKLLTEIRDLLAANNNPAAAP
LAGQPPQYG
>Mature_169_residues
MFGWRKVVSSGRGVVEPPTRLITPGLRGFKQFLMRGNIVDLAIAVVVGTAFTAVVQSLVKNIFTPLIAAIGGEPDFSSLT
FTINDSKFRYGAFINDVIAFIVVAAVIYFVIVLPLAKANEMRRRGEEPEPQEEPAISDEAKLLTEIRDLLAANNNPAAAP
LAGQPPQYG

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=137, Percent_Identity=40.1459854014599, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 18270; Mature: 18270

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGWRKVVSSGRGVVEPPTRLITPGLRGFKQFLMRGNIVDLAIAVVVGTAFTAVVQSLVK
CCHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
NIFTPLIAAIGGEPDFSSLTFTINDSKFRYGAFINDVIAFIVVAAVIYFVIVLPLAKANE
HHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRRRGEEPEPQEEPAISDEAKLLTEIRDLLAANNNPAAAPLAGQPPQYG
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFGWRKVVSSGRGVVEPPTRLITPGLRGFKQFLMRGNIVDLAIAVVVGTAFTAVVQSLVK
CCHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
NIFTPLIAAIGGEPDFSSLTFTINDSKFRYGAFINDVIAFIVVAAVIYFVIVLPLAKANE
HHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRRRGEEPEPQEEPAISDEAKLLTEIRDLLAANNNPAAAPLAGQPPQYG
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA