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The map label for this gene is tnpR [H]

Identifier: 158318112

GI number: 158318112

Start: 7736275

End: 7736895

Strand: Reverse

Name: tnpR [H]

Synonym: Franean1_6376

Alternate gene names: 158318112

Gene position: 7736895-7736275 (Counterclockwise)

Preceding gene: 158318115

Following gene: 158318111

Centisome position: 86.14

GC content: 70.37

Gene sequence:

>621_bases
GTGTCTGCCGCCCCCGCACACCCCCCGACCGGACTCGACCTCGGCTACGCCCGGGTCTCCACCACCAAGCAGAGCCTCGA
GCGCCAGCTCGACGCCCTGGCCGCCGCCGGCATCCCCACCGGGCGCATCTTCACCGACAAGAAGACCGGCGCCACCGTCG
ACCGCGAAGGCCTGACAGCACTGCTCGCCTACGCCCGCCCGGGCGACACGATCGTCGTGCACACACTCGACCGGCTCGGC
CGCAACCTGCGCGAAGTCCTCAACCTCGTCCACGACCTCGCAGAGAAGCACATCGGCGTGCGCTCCCTGGCCGACCCGCT
GCCGATCAACACCGCCGATGAGGGCATGGGCCGCATCGCCTTCCTCCTCCTGGCGCTGTTCGCCGAGATGGAACGCACCT
TCACCGCCGAACGCGCCGCAAACGCACGCGCCGTCGCCGAAGCCAAAGGCCGCCACGTCGGCCGGCCCTTGGCCCACCCC
ACCGGCAAGATCGAATACGCGCGGCTCCTCAAAGCCCGCGGCGACAGCCTCGGCACGATCGCCACCAAGACCGGCATCCC
GAAGACCTCGCTGCACCGCTACCTCGCAGGCAGCGAACCGGCGCCAGCGTCGACACAGTAA

Upstream 100 bases:

>100_bases
CGCGAGGCGGTGAAAACAGGTCCCATATTGTTCCGGAAGTCGTTCCACAACACATGTTCCGAAACAGTAGTACGGAGACG
ACTTCCGGTACGATCTCTGC

Downstream 100 bases:

>100_bases
CCGCCCAACGGACCAGACAGCCGCTACACGACGTCGCCCGACGACCATTGCCCCTTTCCGGCGTATCTCGGCGGCCCCCC
GAGTTCATCCGCGAACTCAT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSAAPAHPPTGLDLGYARVSTTKQSLERQLDALAAAGIPTGRIFTDKKTGATVDREGLTALLAYARPGDTIVVHTLDRLG
RNLREVLNLVHDLAEKHIGVRSLADPLPINTADEGMGRIAFLLLALFAEMERTFTAERAANARAVAEAKGRHVGRPLAHP
TGKIEYARLLKARGDSLGTIATKTGIPKTSLHRYLAGSEPAPASTQ

Sequences:

>Translated_206_residues
MSAAPAHPPTGLDLGYARVSTTKQSLERQLDALAAAGIPTGRIFTDKKTGATVDREGLTALLAYARPGDTIVVHTLDRLG
RNLREVLNLVHDLAEKHIGVRSLADPLPINTADEGMGRIAFLLLALFAEMERTFTAERAANARAVAEAKGRHVGRPLAHP
TGKIEYARLLKARGDSLGTIATKTGIPKTSLHRYLAGSEPAPASTQ
>Mature_205_residues
SAAPAHPPTGLDLGYARVSTTKQSLERQLDALAAAGIPTGRIFTDKKTGATVDREGLTALLAYARPGDTIVVHTLDRLGR
NLREVLNLVHDLAEKHIGVRSLADPLPINTADEGMGRIAFLLLALFAEMERTFTAERAANARAVAEAKGRHVGRPLAHPT
GKIEYARLLKARGDSLGTIATKTGIPKTSLHRYLAGSEPAPASTQ

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=160, Percent_Identity=41.25, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787638, Length=186, Percent_Identity=29.5698924731183, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787827, Length=147, Percent_Identity=32.6530612244898, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21927; Mature: 21796

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAPAHPPTGLDLGYARVSTTKQSLERQLDALAAAGIPTGRIFTDKKTGATVDREGLTA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHH
LLAYARPGDTIVVHTLDRLGRNLREVLNLVHDLAEKHIGVRSLADPLPINTADEGMGRIA
HHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHH
FLLLALFAEMERTFTAERAANARAVAEAKGRHVGRPLAHPTGKIEYARLLKARGDSLGTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHH
ATKTGIPKTSLHRYLAGSEPAPASTQ
HHCCCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SAAPAHPPTGLDLGYARVSTTKQSLERQLDALAAAGIPTGRIFTDKKTGATVDREGLTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHH
LLAYARPGDTIVVHTLDRLGRNLREVLNLVHDLAEKHIGVRSLADPLPINTADEGMGRIA
HHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHH
FLLLALFAEMERTFTAERAANARAVAEAKGRHVGRPLAHPTGKIEYARLLKARGDSLGTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHH
ATKTGIPKTSLHRYLAGSEPAPASTQ
HHCCCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]