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The map label for this gene is tcmG [H]

Identifier: 158318077

GI number: 158318077

Start: 7698385

End: 7699899

Strand: Direct

Name: tcmG [H]

Synonym: Franean1_6338

Alternate gene names: 158318077

Gene position: 7698385-7699899 (Clockwise)

Preceding gene: 158318074

Following gene: 158318078

Centisome position: 85.71

GC content: 71.42

Gene sequence:

>1515_bases
ATGGCATCTGAGGTGGTCGAGACACCCGTGCTGATCGTCGGCGGCGGCCCGGCCGGGCTGACCGCATCCCTGCTGCTGTC
CCGCCACGGCCTAGGCTCCCTGCTGATCGACAAGCGCCTCGACGCGTCACCGCTGCCCCGCGCCCGTGGCGTGCACTCAC
GGGCGATGGAGATCCTGCGCGTCTGCGGCGTCGAACCGGATCTGCGCAAGGTGGAGCTACCGATCACCCCAGGCGCGGAA
TGGCGAGCCGATCTGACCAGCCCGCCGCTGCGCGAGGACGTACCGTCGGCCGGTCCCACCACTGTGAGCCCATGCGAGGG
ACTGTCGGTCTCGCAGGACGTGTTCGAGGCGGTCCTGCGAGACCACGCCCACAGCTACGCACACGCGCAGCTGCGACCGG
GCACGCTGCTGGACTCGTTCCACACCGCCGGTGACGGTGTCCTGGCCACCGTGGTCGAACGGGCGTCCGGACACCGCGTC
GAGGTGCGGGCCCGGTGGATGATCGCCGCCGATGGTGCCCGCAGCGACATACGCCGGCACCTGCGGATCGGCATGAACGG
CCCGGACGACCTCGGCCAGCAGACGATGATCGCCTTCCGGGCCGACCTGAGCGCATTGACCGGTTCGCGTCCCCGAGGGA
TCTACTTCCTCACCGCCACCGGCGCAGCGCTGATCTGGACCCACCACGACGACCGCTGGGTGATCAGCCAACCCGGCCCC
ATCGCCGCCGACGATGATCCCGCCGCCATCGTCCGGCGGATCCTCGGCCTTGCCGATCTGACCGTGCAGGTGCTGGCCAC
CAGCCCCTGGACGGCCGCGGCGCAGTCGGCCGCCCAGTACGCGCAAGGCCCTGTGTTCCTGGCCGGCGACGCCGCCCATC
GCTTCCCGCCCGCCGGCGCGACCGGGGTCAGCGCGGCGATGCACGACGTGCACAACCTCGCCTGGAAGATCGCCGCCGTC
GCCCACGGCCACAGCGGCCAGAAGCTGCTGGACAGTTACGCCGCCGAACGAGAACCGGTCGGGCAGCGCACCGCCGACGA
GACCGGCGCCGCATGGACGCGGATCTTCAACGGGAACGCCGCGCCGTTCAGCGGACGCAGCCTTGCCCAGATCGATATGG
GCTACCACTACACGTCACCGGTCGTCGTCGACGACGGCAGCCCCGACGCCGACCCGCCTGGCGCCGGCTACCAGCCGACC
GCTACACCCGGCTGCCGGGCGCCCCATCTGTGGCTGGCCGACGGCACGTCCACCATCGACCTGTTCGACCAGCGCTTCGT
CCTGCTGACCGCCGAACCCGGCCACGCCTGGCGCGACGCCGCCGCCCACGCCACCGGGGCACTCGTCGACAGCCACGTCA
TCGCAGAACCGAACTGGCCGAGCCTGTACGGCGTCGCCCCCGACGGGGCGGTCCTCGTTCGCCCCGACGGGCATGTCGCG
TGGCGCAGCCGGACCTCATCCACCGATCCGGTGACGGACATCCAGACCGCCCTGACCACCAGCACCGGAAGCTGA

Upstream 100 bases:

>100_bases
CCTGACGCTGCCGGTGGGTCGGCCCTTCCCTCTTGACAGCCACCGGCGGACAGTATCACTCTAAGAATCATAGTGTCGCT
ATGACGAAGGGAGCGTCGCT

Downstream 100 bases:

>100_bases
CCCCGGCGCACCACCAGCCAGACGACGGACTTTCCGCAGACGTCGGCGACGTGGCCGGGGAACGGCCAGGCCAGGCGCCG
GAGGAACCGATCAGGGATGC

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MASEVVETPVLIVGGGPAGLTASLLLSRHGLGSLLIDKRLDASPLPRARGVHSRAMEILRVCGVEPDLRKVELPITPGAE
WRADLTSPPLREDVPSAGPTTVSPCEGLSVSQDVFEAVLRDHAHSYAHAQLRPGTLLDSFHTAGDGVLATVVERASGHRV
EVRARWMIAADGARSDIRRHLRIGMNGPDDLGQQTMIAFRADLSALTGSRPRGIYFLTATGAALIWTHHDDRWVISQPGP
IAADDDPAAIVRRILGLADLTVQVLATSPWTAAAQSAAQYAQGPVFLAGDAAHRFPPAGATGVSAAMHDVHNLAWKIAAV
AHGHSGQKLLDSYAAEREPVGQRTADETGAAWTRIFNGNAAPFSGRSLAQIDMGYHYTSPVVVDDGSPDADPPGAGYQPT
ATPGCRAPHLWLADGTSTIDLFDQRFVLLTAEPGHAWRDAAAHATGALVDSHVIAEPNWPSLYGVAPDGAVLVRPDGHVA
WRSRTSSTDPVTDIQTALTTSTGS

Sequences:

>Translated_504_residues
MASEVVETPVLIVGGGPAGLTASLLLSRHGLGSLLIDKRLDASPLPRARGVHSRAMEILRVCGVEPDLRKVELPITPGAE
WRADLTSPPLREDVPSAGPTTVSPCEGLSVSQDVFEAVLRDHAHSYAHAQLRPGTLLDSFHTAGDGVLATVVERASGHRV
EVRARWMIAADGARSDIRRHLRIGMNGPDDLGQQTMIAFRADLSALTGSRPRGIYFLTATGAALIWTHHDDRWVISQPGP
IAADDDPAAIVRRILGLADLTVQVLATSPWTAAAQSAAQYAQGPVFLAGDAAHRFPPAGATGVSAAMHDVHNLAWKIAAV
AHGHSGQKLLDSYAAEREPVGQRTADETGAAWTRIFNGNAAPFSGRSLAQIDMGYHYTSPVVVDDGSPDADPPGAGYQPT
ATPGCRAPHLWLADGTSTIDLFDQRFVLLTAEPGHAWRDAAAHATGALVDSHVIAEPNWPSLYGVAPDGAVLVRPDGHVA
WRSRTSSTDPVTDIQTALTTSTGS
>Mature_503_residues
ASEVVETPVLIVGGGPAGLTASLLLSRHGLGSLLIDKRLDASPLPRARGVHSRAMEILRVCGVEPDLRKVELPITPGAEW
RADLTSPPLREDVPSAGPTTVSPCEGLSVSQDVFEAVLRDHAHSYAHAQLRPGTLLDSFHTAGDGVLATVVERASGHRVE
VRARWMIAADGARSDIRRHLRIGMNGPDDLGQQTMIAFRADLSALTGSRPRGIYFLTATGAALIWTHHDDRWVISQPGPI
AADDDPAAIVRRILGLADLTVQVLATSPWTAAAQSAAQYAQGPVFLAGDAAHRFPPAGATGVSAAMHDVHNLAWKIAAVA
HGHSGQKLLDSYAAEREPVGQRTADETGAAWTRIFNGNAAPFSGRSLAQIDMGYHYTSPVVVDDGSPDADPPGAGYQPTA
TPGCRAPHLWLADGTSTIDLFDQRFVLLTAEPGHAWRDAAAHATGALVDSHVIAEPNWPSLYGVAPDGAVLVRPDGHVAW
RSRTSSTDPVTDIQTALTTSTGS

Specific function: Hydroxylates tetracenomycin A2 at positions C-4, C-4A, and C12A to give tetracenomycin C (TCM C). Hydroxylates as well 9- carboxymethyl-TCM B3 to yield 8-demethyl-TMC C, a side reaction [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 53205; Mature: 53074

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEVVETPVLIVGGGPAGLTASLLLSRHGLGSLLIDKRLDASPLPRARGVHSRAMEILR
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHH
VCGVEPDLRKVELPITPGAEWRADLTSPPLREDVPSAGPTTVSPCEGLSVSQDVFEAVLR
HHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
DHAHSYAHAQLRPGTLLDSFHTAGDGVLATVVERASGHRVEVRARWMIAADGARSDIRRH
HHHHHHHEEECCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCHHHHHHH
LRIGMNGPDDLGQQTMIAFRADLSALTGSRPRGIYFLTATGAALIWTHHDDRWVISQPGP
HEECCCCCHHHCHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEECCCEEEEECCCC
IAADDDPAAIVRRILGLADLTVQVLATSPWTAAAQSAAQYAQGPVFLAGDAAHRFPPAGA
CCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHCCCCCCC
TGVSAAMHDVHNLAWKIAAVAHGHSGQKLLDSYAAEREPVGQRTADETGAAWTRIFNGNA
CHHHHHHHHHHHHHHEEEHEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
APFSGRSLAQIDMGYHYTSPVVVDDGSPDADPPGAGYQPTATPGCRAPHLWLADGTSTID
CCCCCCCEEEEECCEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE
LFDQRFVLLTAEPGHAWRDAAAHATGALVDSHVIAEPNWPSLYGVAPDGAVLVRPDGHVA
ECCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCCEEEECCCCCEE
WRSRTSSTDPVTDIQTALTTSTGS
ECCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
ASEVVETPVLIVGGGPAGLTASLLLSRHGLGSLLIDKRLDASPLPRARGVHSRAMEILR
CCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHH
VCGVEPDLRKVELPITPGAEWRADLTSPPLREDVPSAGPTTVSPCEGLSVSQDVFEAVLR
HHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
DHAHSYAHAQLRPGTLLDSFHTAGDGVLATVVERASGHRVEVRARWMIAADGARSDIRRH
HHHHHHHEEECCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCHHHHHHH
LRIGMNGPDDLGQQTMIAFRADLSALTGSRPRGIYFLTATGAALIWTHHDDRWVISQPGP
HEECCCCCHHHCHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEECCCEEEEECCCC
IAADDDPAAIVRRILGLADLTVQVLATSPWTAAAQSAAQYAQGPVFLAGDAAHRFPPAGA
CCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHCCCCCCC
TGVSAAMHDVHNLAWKIAAVAHGHSGQKLLDSYAAEREPVGQRTADETGAAWTRIFNGNA
CHHHHHHHHHHHHHHEEEHEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
APFSGRSLAQIDMGYHYTSPVVVDDGSPDADPPGAGYQPTATPGCRAPHLWLADGTSTID
CCCCCCCEEEEECCEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE
LFDQRFVLLTAEPGHAWRDAAAHATGALVDSHVIAEPNWPSLYGVAPDGAVLVRPDGHVA
ECCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCCEEEECCCCCEE
WRSRTSSTDPVTDIQTALTTSTGS
ECCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]