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The map label for this gene is gsiB [H]

Identifier: 158317996

GI number: 158317996

Start: 7597411

End: 7598979

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_6256

Alternate gene names: 158317996

Gene position: 7598979-7597411 (Counterclockwise)

Preceding gene: 158318003

Following gene: 158317995

Centisome position: 84.6

GC content: 67.43

Gene sequence:

>1569_bases
ATGATTCACCGCGCGCGATTACTCGTCACCGCTCTCGTCTGCTGTACGGGTCTGCTGCTGGGGGCCTGCGGGGGAGGAGG
TGGCGATACCGGTACGTCCGCGTCGGGGGCCACGGGTGAGCCTGTGGCGGGTGGCGAAGCGCGGATCCTGCTGCTGAGCG
AGCCGCGCACGATGGACCCGGCGGTCATCGGCAACGTCTACGCCAGCGGAGCCCTTATCGGGAACGCCCTGTACGGAACA
CTGATGACCGACGACGAGGCGGGTGAGATCCACTACTCGATGGCCGAGTCGTTCGAGTCCGCCGACAACGGCAAGACGTT
CGAGCTGAAGCTGCGGCCGGGGCTGGTGTTCTCGGACGGTACTCCGCTGAATGCGGATGCCGTGAAGTTCAACTGGGACC
GGACCAAGGAACCGGCCACAGGCTCCGCCAGCCGGGCGGATGCTGCGATGATCGCCTCGTCCGAGGTGGTTGACGACGTC
ACGCTGCGGGTCACCCTGGTCACCCCCGTGCCGAAGTACTCCGAGGCCATCGTCACCTCGACGCTGAACTGGATCGCCTC
GCCGGCGGCCCTGCAGAAGGGCGGGCCGGCCTTCGACAAGAACCCGATCGGCGCCGGGCCGTTCACCCTGCAGAGCTGGG
CCCGCCAGGACAACATGCGGCTGGTCAAGAACCCCCGCTACTGGGACGCGCCCAAGCCGTACCTCGACTCCCTCGTCCTG
CGCCCGGCACAGGACAGCAACCAGCGCTACAACACGCTGCTCACCGGTGGTGCCGACCTGGCGGTCGACTCAAGCTGGAT
CAACCTCGGCAAGGCTGACGAGGCGGGGCTGTCGGTCGACTTCCTGCCGGCCAGCGGCGGCATCGTCGCCGCGCTGAACA
CGCGCCGGGCGCCGTTCGACGACGTGCGCGCACGTCAGGCGGTCGCGAAGGCGCTGGACATGGACGCGCTGAACCTCGCC
GTCTGGAACGGCAGCGCGCGGATGGCCACCACGCTGTTCACCGACTCATCACCGTTCTACTCGCCCACGCCGTTGCAGAA
GACGGACAAGGCGACCGCCCAGAAGCTCTTCGACGAGCTCGCCGCCGAGGGCAAGCCGGTCTCCTTCTCGTTCACCACCA
CTCCGGCGTCGGAGAACCGGAAGATGGCCGAGAACATCCAGGCCCAGCTGAGCGCGTTCAAGAACGTCAAGTTCCAGGTC
CGGGTCATAGAGATCGCCGAGTTCTCGGCGCTGCGCACGTCCCACGACTACGACGCGGTCCTCACGTCCTCGATGTTCCT
GGACCCCGACCCCCGGCTGCCGACGACCCTCCTCGGGGGCTCGTCGGCGAACCTGTCCATGCTCGACGATCCTCAGCTGA
ACGAGAGCCTGCTGGCGGGACGGACCGCGACCACCGTGGAGGAGCGGAAGAAGGCCTATGACCGAGTGCAGGCCCGGCTG
ACGGAGGTGGTCCCGATGATCTTCTTCGCGCGCGGGCCGCTCGGCGCCATCTCGGCCAGGAACGTCGGCGGTGTCACGCA
GTACGGCCTTGGATCGCTGCTGCCGGAGAAGCTGTGGATCCAGTCATGA

Upstream 100 bases:

>100_bases
GATAGCCAGGGCCGTCAGGCAGGTGCCGCTCCAGCCGCACATTCCCAACTATGATCTTGCCGGATTTTGTTGATCATGAA
AACCGATGAAGGGGTCGAAG

Downstream 100 bases:

>100_bases
CGGCCTGACAGCGGCACAACGTACATCGCCTGACGGCCGTCACCTGGCGCGCGTCGATCATCGTCGGCGCGCGCCAGGGT
GGCTTCGGGCGGCGGTGCCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MIHRARLLVTALVCCTGLLLGACGGGGGDTGTSASGATGEPVAGGEARILLLSEPRTMDPAVIGNVYASGALIGNALYGT
LMTDDEAGEIHYSMAESFESADNGKTFELKLRPGLVFSDGTPLNADAVKFNWDRTKEPATGSASRADAAMIASSEVVDDV
TLRVTLVTPVPKYSEAIVTSTLNWIASPAALQKGGPAFDKNPIGAGPFTLQSWARQDNMRLVKNPRYWDAPKPYLDSLVL
RPAQDSNQRYNTLLTGGADLAVDSSWINLGKADEAGLSVDFLPASGGIVAALNTRRAPFDDVRARQAVAKALDMDALNLA
VWNGSARMATTLFTDSSPFYSPTPLQKTDKATAQKLFDELAAEGKPVSFSFTTTPASENRKMAENIQAQLSAFKNVKFQV
RVIEIAEFSALRTSHDYDAVLTSSMFLDPDPRLPTTLLGGSSANLSMLDDPQLNESLLAGRTATTVEERKKAYDRVQARL
TEVVPMIFFARGPLGAISARNVGGVTQYGLGSLLPEKLWIQS

Sequences:

>Translated_522_residues
MIHRARLLVTALVCCTGLLLGACGGGGGDTGTSASGATGEPVAGGEARILLLSEPRTMDPAVIGNVYASGALIGNALYGT
LMTDDEAGEIHYSMAESFESADNGKTFELKLRPGLVFSDGTPLNADAVKFNWDRTKEPATGSASRADAAMIASSEVVDDV
TLRVTLVTPVPKYSEAIVTSTLNWIASPAALQKGGPAFDKNPIGAGPFTLQSWARQDNMRLVKNPRYWDAPKPYLDSLVL
RPAQDSNQRYNTLLTGGADLAVDSSWINLGKADEAGLSVDFLPASGGIVAALNTRRAPFDDVRARQAVAKALDMDALNLA
VWNGSARMATTLFTDSSPFYSPTPLQKTDKATAQKLFDELAAEGKPVSFSFTTTPASENRKMAENIQAQLSAFKNVKFQV
RVIEIAEFSALRTSHDYDAVLTSSMFLDPDPRLPTTLLGGSSANLSMLDDPQLNESLLAGRTATTVEERKKAYDRVQARL
TEVVPMIFFARGPLGAISARNVGGVTQYGLGSLLPEKLWIQS
>Mature_522_residues
MIHRARLLVTALVCCTGLLLGACGGGGGDTGTSASGATGEPVAGGEARILLLSEPRTMDPAVIGNVYASGALIGNALYGT
LMTDDEAGEIHYSMAESFESADNGKTFELKLRPGLVFSDGTPLNADAVKFNWDRTKEPATGSASRADAAMIASSEVVDDV
TLRVTLVTPVPKYSEAIVTSTLNWIASPAALQKGGPAFDKNPIGAGPFTLQSWARQDNMRLVKNPRYWDAPKPYLDSLVL
RPAQDSNQRYNTLLTGGADLAVDSSWINLGKADEAGLSVDFLPASGGIVAALNTRRAPFDDVRARQAVAKALDMDALNLA
VWNGSARMATTLFTDSSPFYSPTPLQKTDKATAQKLFDELAAEGKPVSFSFTTTPASENRKMAENIQAQLSAFKNVKFQV
RVIEIAEFSALRTSHDYDAVLTSSMFLDPDPRLPTTLLGGSSANLSMLDDPQLNESLLAGRTATTVEERKKAYDRVQARL
TEVVPMIFFARGPLGAISARNVGGVTQYGLGSLLPEKLWIQS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=364, Percent_Identity=30.4945054945055, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787495, Length=477, Percent_Identity=24.9475890985325, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789397, Length=490, Percent_Identity=25.1020408163265, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1789966, Length=469, Percent_Identity=25.1599147121535, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081878, Length=508, Percent_Identity=23.2283464566929, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787762, Length=353, Percent_Identity=25.2124645892351, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787551, Length=459, Percent_Identity=24.1830065359477, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789887, Length=360, Percent_Identity=25.2777777777778, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55633; Mature: 55633

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHRARLLVTALVCCTGLLLGACGGGGGDTGTSASGATGEPVAGGEARILLLSEPRTMDP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCC
AVIGNVYASGALIGNALYGTLMTDDEAGEIHYSMAESFESADNGKTFELKLRPGLVFSDG
HHHHHHHHCCEEECHHHEEEEECCCCCCCEEHHHHHHHHCCCCCCEEEEEECCCEEECCC
TPLNADAVKFNWDRTKEPATGSASRADAAMIASSEVVDDVTLRVTLVTPVPKYSEAIVTS
CCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHCCEEEEEEEECCCCCHHHHHHHH
TLNWIASPAALQKGGPAFDKNPIGAGPFTLQSWARQDNMRLVKNPRYWDAPKPYLDSLVL
HHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHEE
RPAQDSNQRYNTLLTGGADLAVDSSWINLGKADEAGLSVDFLPASGGIVAALNTRRAPFD
CCCCCCCCCEEEEEECCCCEEECCCHHCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHH
DVRARQAVAKALDMDALNLAVWNGSARMATTLFTDSSPFYSPTPLQKTDKATAQKLFDEL
HHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
AAEGKPVSFSFTTTPASENRKMAENIQAQLSAFKNVKFQVRVIEIAEFSALRTSHDYDAV
HCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEEHHHHHHCCCCCHHH
LTSSMFLDPDPRLPTTLLGGSSANLSMLDDPQLNESLLAGRTATTVEERKKAYDRVQARL
HHHCEEECCCCCCCEEEECCCCCCCEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
TEVVPMIFFARGPLGAISARNVGGVTQYGLGSLLPEKLWIQS
HHHHHHHHHCCCCCCCCCCCCCCCCHHHCHHHHCCHHHCCCC
>Mature Secondary Structure
MIHRARLLVTALVCCTGLLLGACGGGGGDTGTSASGATGEPVAGGEARILLLSEPRTMDP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCC
AVIGNVYASGALIGNALYGTLMTDDEAGEIHYSMAESFESADNGKTFELKLRPGLVFSDG
HHHHHHHHCCEEECHHHEEEEECCCCCCCEEHHHHHHHHCCCCCCEEEEEECCCEEECCC
TPLNADAVKFNWDRTKEPATGSASRADAAMIASSEVVDDVTLRVTLVTPVPKYSEAIVTS
CCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHCCEEEEEEEECCCCCHHHHHHHH
TLNWIASPAALQKGGPAFDKNPIGAGPFTLQSWARQDNMRLVKNPRYWDAPKPYLDSLVL
HHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHEE
RPAQDSNQRYNTLLTGGADLAVDSSWINLGKADEAGLSVDFLPASGGIVAALNTRRAPFD
CCCCCCCCCEEEEEECCCCEEECCCHHCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHH
DVRARQAVAKALDMDALNLAVWNGSARMATTLFTDSSPFYSPTPLQKTDKATAQKLFDEL
HHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
AAEGKPVSFSFTTTPASENRKMAENIQAQLSAFKNVKFQVRVIEIAEFSALRTSHDYDAV
HCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEEHHHHHHCCCCCHHH
LTSSMFLDPDPRLPTTLLGGSSANLSMLDDPQLNESLLAGRTATTVEERKKAYDRVQARL
HHHCEEECCCCCCCEEEECCCCCCCEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
TEVVPMIFFARGPLGAISARNVGGVTQYGLGSLLPEKLWIQS
HHHHHHHHHCCCCCCCCCCCCCCCCHHHCHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA