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The map label for this gene is nuoB

Identifier: 158317835

GI number: 158317835

Start: 7417576

End: 7418370

Strand: Reverse

Name: nuoB

Synonym: Franean1_6093

Alternate gene names: 158317835

Gene position: 7418370-7417576 (Counterclockwise)

Preceding gene: 158317836

Following gene: 158317834

Centisome position: 82.59

GC content: 70.69

Gene sequence:

>795_bases
ATGGGACTAGAAGAGAAGCTGCCCGGTGGAGTTCTGCTGACCACCGTCGAGAAGCTGGCCAACTGGTCGCGCAGGTCGTC
GCTGTGGCCGGCGACCTTCGGCCTGGCCTGCTGTGCCATCGAGATGATGTCGACCGGCGCCGGCCGGTACGACCTCTCCC
GCTTCGGGATGGAGGTCTTCCGCGCCAGCCCCCGCCAGGCCGACCTCATGATCGTGGCGGGCCGGGTGAGCCAGAAGATG
GCCCCGGTGCTGCGCCAGATCTACGACCAGATGCCCGAGCCCAAGTGGGTGCTGTCGATGGGCGTCTGCGCGAGCAGCGG
CGGCATGTTCAACAACTACGCGATCGTGCAGGGCGTCGACCACATCGTCCCGGTGGACATGTACCTGCCGGGCTGCCCGC
CGCGTCCCGAGATGCTGATGGACGCGATCGTCAAGCTGCACGAGAAGATCCTCGCCGGCTCGGTGTCGGGCAAGGTCGAG
CACACCAAGGTCGGCTCGGCGCCCTACCCGAAGCCCATCGAGGTGGCCACCGCGTGGTCCGGCCTGCCCGCCGGCGCGGT
CGACACCCGCACGCTGTCGGTCAACGACCGCAAGCGGTTCAGCCTCCCCGAGGACGCTCCGGTCCCGGCCGGGCGCGGTG
CCGTCGAGCCGGTGCTGGACGAGCGCCGCCCGGCGGCGATCGCGCCGCCGTCCGTCTTCGGCCGCGGCAAGCGGGTGCCC
GTCGACGCCAAGCCGACCGACGAGTCCCGGGCCGGCGGTCCCGGACCGAGCACGGAGACCCTGACCGATGACTGA

Upstream 100 bases:

>100_bases
CGTTCGACAGTCTCGCGGTCTTCGGTCTCGGCGCGATGGTGTCGTTCATGGCGACGATCTTCGTCGCCTACGCCTACATA
TGGCGCCGGGGGGGTCTGGA

Downstream 100 bases:

>100_bases
CCTGGTACCGGGCTCCGCCGGCGTTCCGGGCGGCCCGGGCGGCCGCCGCCGGGACGCCTTCGGCGCGCGCGGCAGCGGCG
ACACGTCGGGTTTCGGCGGC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGLEEKLPGGVLLTTVEKLANWSRRSSLWPATFGLACCAIEMMSTGAGRYDLSRFGMEVFRASPRQADLMIVAGRVSQKM
APVLRQIYDQMPEPKWVLSMGVCASSGGMFNNYAIVQGVDHIVPVDMYLPGCPPRPEMLMDAIVKLHEKILAGSVSGKVE
HTKVGSAPYPKPIEVATAWSGLPAGAVDTRTLSVNDRKRFSLPEDAPVPAGRGAVEPVLDERRPAAIAPPSVFGRGKRVP
VDAKPTDESRAGGPGPSTETLTDD

Sequences:

>Translated_264_residues
MGLEEKLPGGVLLTTVEKLANWSRRSSLWPATFGLACCAIEMMSTGAGRYDLSRFGMEVFRASPRQADLMIVAGRVSQKM
APVLRQIYDQMPEPKWVLSMGVCASSGGMFNNYAIVQGVDHIVPVDMYLPGCPPRPEMLMDAIVKLHEKILAGSVSGKVE
HTKVGSAPYPKPIEVATAWSGLPAGAVDTRTLSVNDRKRFSLPEDAPVPAGRGAVEPVLDERRPAAIAPPSVFGRGKRVP
VDAKPTDESRAGGPGPSTETLTDD
>Mature_263_residues
GLEEKLPGGVLLTTVEKLANWSRRSSLWPATFGLACCAIEMMSTGAGRYDLSRFGMEVFRASPRQADLMIVAGRVSQKMA
PVLRQIYDQMPEPKWVLSMGVCASSGGMFNNYAIVQGVDHIVPVDMYLPGCPPRPEMLMDAIVKLHEKILAGSVSGKVEH
TKVGSAPYPKPIEVATAWSGLPAGAVDTRTLSVNDRKRFSLPEDAPVPAGRGAVEPVLDERRPAAIAPPSVFGRGKRVPV
DAKPTDESRAGGPGPSTETLTDD

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=59.4405594405594, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1788624, Length=148, Percent_Identity=54.7297297297297, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1789074, Length=105, Percent_Identity=43.8095238095238, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788834, Length=125, Percent_Identity=37.6, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17509685, Length=139, Percent_Identity=56.1151079136691, Blast_Score=176, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24651058, Length=151, Percent_Identity=56.2913907284768, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=57.5539568345324, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=57.5539568345324, Blast_Score=185, Evalue=2e-47,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_FRASN (A8LCA0)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001510343.1
- ProteinModelPortal:   A8LCA0
- SMR:   A8LCA0
- GeneID:   5674414
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_6093
- HOGENOM:   HBG553221
- OMA:   MSTGAGR
- ProtClustDB:   PRK06411
- BioCyc:   FSP1855:FRANEAN1_6093-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 28282; Mature: 28151

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLEEKLPGGVLLTTVEKLANWSRRSSLWPATFGLACCAIEMMSTGAGRYDLSRFGMEVF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RASPRQADLMIVAGRVSQKMAPVLRQIYDQMPEPKWVLSMGVCASSGGMFNNYAIVQGVD
HCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCCCCCCCCCHHHHCCC
HIVPVDMYLPGCPPRPEMLMDAIVKLHEKILAGSVSGKVEHTKVGSAPYPKPIEVATAWS
CEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCC
GLPAGAVDTRTLSVNDRKRFSLPEDAPVPAGRGAVEPVLDERRPAAIAPPSVFGRGKRVP
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCCC
VDAKPTDESRAGGPGPSTETLTDD
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GLEEKLPGGVLLTTVEKLANWSRRSSLWPATFGLACCAIEMMSTGAGRYDLSRFGMEVF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RASPRQADLMIVAGRVSQKMAPVLRQIYDQMPEPKWVLSMGVCASSGGMFNNYAIVQGVD
HCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCCCCCCCCCHHHHCCC
HIVPVDMYLPGCPPRPEMLMDAIVKLHEKILAGSVSGKVEHTKVGSAPYPKPIEVATAWS
CEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCC
GLPAGAVDTRTLSVNDRKRFSLPEDAPVPAGRGAVEPVLDERRPAAIAPPSVFGRGKRVP
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCCC
VDAKPTDESRAGGPGPSTETLTDD
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA