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The map label for this gene is truA [H]

Identifier: 158317762

GI number: 158317762

Start: 7342901

End: 7343728

Strand: Reverse

Name: truA [H]

Synonym: Franean1_6020

Alternate gene names: 158317762

Gene position: 7343728-7342901 (Counterclockwise)

Preceding gene: 158317763

Following gene: 158317760

Centisome position: 81.76

GC content: 76.57

Gene sequence:

>828_bases
ATGAGACCGGCCGAGGGACTGATCCGGCTGCGCCTGGACCTCGCCTACGACGGCACGGCCTTCGCCGGCTGGGCCCGCCA
GCCCGGCCAGCGGACGGTCCAGGGTGACGTCGAGGACGCGCTGATGCGGGTCGCGCGGCTGCCGGCCGTCCGGCTCACCG
TCGCCGGGCGCACCGACTCCGGCGTGCACGCCGCCGGCCAGGTGGCCCACGTCGACATCCCAGACGACACGCCGGTCGGT
GGGCTCGCCCGCAGGCTGAACGGCGTGCTCGACCGCGCCGTCCGGGTGAAGGGCCTCGCGCCCGCCCCGGACGGGTTTGA
CGCCCGCTTCTCCGCGCTGTCCCGCAGATACGTCTACCGGATCACCGACGCCCCGTACGGGGCGGACCCGCTGCGCCGTT
TCGACACCCTGGCGTCGCCGCGGCCGCTGGACGTCCCGGCGATGGCCGAGGCGGCGCTGCTGCTGCTCGGCGAGCATGAC
TTCGCCGCCTTCTGCCGGCGGCGCGAGGGCGCCACGACCGTCCGCACCCTGCTGCGCCTGGACGTCTCCCGCTCCCGCGA
CGTCATCCTCGCCGACGTCGAGGCGGACGCGTTCTGCCACTCCATGGTGCGTGCCCTGGCCGGCGCGCTGGTGGCGGTGG
GGGAGGGCCGGCGCCGGCCGGAGTGGGTGGCCGCCGTCCTGGGCCGGGGCATGCGAGATCCCGCGGTGACTGTACTTCCG
GCCCACGGGCTGACCCTGGTGGAGGTCCGCTACCCGCCGGACGCCGCCCTCGCCGCGCGGGCCCAGGTGACCCGCGCGGT
CCGCGTGCCGACGACGCCGTCCGGCTAG

Upstream 100 bases:

>100_bases
CTCCGGGAACCAGGCCGGCCATGGGAGCCAGGGTGGCTTCGGGAAGGCCGTGCGCCGGGTTGATCGGCCGGCGCAGCTTC
GACACGAGGGACGCGCGCTC

Downstream 100 bases:

>100_bases
CGTCTGGCGACGGTCCGCCAGACGGTCGCTTCGGCCGTCCGGCCAGCAGTGCCTCCGGCCGGACGGCCGGAGGCGTGCGT
GCCTTTCGCGTGGCTGACCC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRPAEGLIRLRLDLAYDGTAFAGWARQPGQRTVQGDVEDALMRVARLPAVRLTVAGRTDSGVHAAGQVAHVDIPDDTPVG
GLARRLNGVLDRAVRVKGLAPAPDGFDARFSALSRRYVYRITDAPYGADPLRRFDTLASPRPLDVPAMAEAALLLLGEHD
FAAFCRRREGATTVRTLLRLDVSRSRDVILADVEADAFCHSMVRALAGALVAVGEGRRRPEWVAAVLGRGMRDPAVTVLP
AHGLTLVEVRYPPDAALAARAQVTRAVRVPTTPSG

Sequences:

>Translated_275_residues
MRPAEGLIRLRLDLAYDGTAFAGWARQPGQRTVQGDVEDALMRVARLPAVRLTVAGRTDSGVHAAGQVAHVDIPDDTPVG
GLARRLNGVLDRAVRVKGLAPAPDGFDARFSALSRRYVYRITDAPYGADPLRRFDTLASPRPLDVPAMAEAALLLLGEHD
FAAFCRRREGATTVRTLLRLDVSRSRDVILADVEADAFCHSMVRALAGALVAVGEGRRRPEWVAAVLGRGMRDPAVTVLP
AHGLTLVEVRYPPDAALAARAQVTRAVRVPTTPSG
>Mature_275_residues
MRPAEGLIRLRLDLAYDGTAFAGWARQPGQRTVQGDVEDALMRVARLPAVRLTVAGRTDSGVHAAGQVAHVDIPDDTPVG
GLARRLNGVLDRAVRVKGLAPAPDGFDARFSALSRRYVYRITDAPYGADPLRRFDTLASPRPLDVPAMAEAALLLLGEHD
FAAFCRRREGATTVRTLLRLDVSRSRDVILADVEADAFCHSMVRALAGALVAVGEGRRRPEWVAAVLGRGMRDPAVTVLP
AHGLTLVEVRYPPDAALAARAQVTRAVRVPTTPSG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=284, Percent_Identity=36.9718309859155, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI199559785, Length=290, Percent_Identity=25.8620689655172, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=36.4, Blast_Score=144, Evalue=6e-36,
Organism=Drosophila melanogaster, GI116007752, Length=308, Percent_Identity=26.2987012987013, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29531; Mature: 29531

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAEGLIRLRLDLAYDGTAFAGWARQPGQRTVQGDVEDALMRVARLPAVRLTVAGRTDS
CCCCCCEEEEEEEEEECCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCC
GVHAAGQVAHVDIPDDTPVGGLARRLNGVLDRAVRVKGLAPAPDGFDARFSALSRRYVYR
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCEEEE
ITDAPYGADPLRRFDTLASPRPLDVPAMAEAALLLLGEHDFAAFCRRREGATTVRTLLRL
ECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHEECCCHHHHHHHHCCCHHHHHHHHHH
DVSRSRDVILADVEADAFCHSMVRALAGALVAVGEGRRRPEWVAAVLGRGMRDPAVTVLP
HCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEE
AHGLTLVEVRYPPDAALAARAQVTRAVRVPTTPSG
CCCEEEEEEECCCCHHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure
MRPAEGLIRLRLDLAYDGTAFAGWARQPGQRTVQGDVEDALMRVARLPAVRLTVAGRTDS
CCCCCCEEEEEEEEEECCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCC
GVHAAGQVAHVDIPDDTPVGGLARRLNGVLDRAVRVKGLAPAPDGFDARFSALSRRYVYR
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCEEEE
ITDAPYGADPLRRFDTLASPRPLDVPAMAEAALLLLGEHDFAAFCRRREGATTVRTLLRL
ECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHEECCCHHHHHHHHCCCHHHHHHHHHH
DVSRSRDVILADVEADAFCHSMVRALAGALVAVGEGRRRPEWVAAVLGRGMRDPAVTVLP
HCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEE
AHGLTLVEVRYPPDAALAARAQVTRAVRVPTTPSG
CCCEEEEEEECCCCHHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA