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The map label for this gene is actII-2 [H]

Identifier: 158317499

GI number: 158317499

Start: 6988186

End: 6989646

Strand: Reverse

Name: actII-2 [H]

Synonym: Franean1_5751

Alternate gene names: 158317499

Gene position: 6989646-6988186 (Counterclockwise)

Preceding gene: 158317501

Following gene: 158317498

Centisome position: 77.82

GC content: 71.25

Gene sequence:

>1461_bases
ATGACCTCTGGCACGAGCACGCCCACCGCAACCGGGGAAGCATTGGCAGACCCGACTACGGCGGCCGAGGCCACAGCCTG
GGGCACGCTGGCCGTGCTGATGGTCGGAACCTTCATCACCGTCCTCGACTACTTCATCGCCAACGTCGCCGCGCCGTCCG
TCCAGCGTGACCTGAACGCCAGCTCGGCGCAGATCCAGGGCGTGATTGTCGGCTACGGCGTCGCGTTCACCGCCGGCATG
ATCACCGGCGGCCGGCTGGGCGACGTCTACGGCCGTCGGCGCATGTTCGCGCTGGGCCTCCTGCTGTTCACGGTGGCCTC
GGCGGCCTGTGGCGTCGCGCCCAACGCCGAAGCGCTGATCGTCGCTCGGGTGGTACAGGGAACCGCCGCCGCGCTGATGG
TTCCGCAGGTGCTCGGCATCTTGGGCACGGTCTACACCGGGCCGAGTCGGGCGCAGGCATTCAACGTGTACGGCCTCGTC
ATCGGGCTTGCCGGCGTGTTCGGGCAGGTGATCGGCGGAGTGCTCATCACGGTCGACGTCGCGGGCCTCGACTGGCGGGC
GGTGTTCCTGCTGAACGTGCCGATCTGCCTGGTGGCGTTGGCCTACACCTACCGCGTGGTGCCCGAGTCGCGCGGCTCGG
CCCGCACGCGTCTCGACCTGACCGGCGCGGCGTTGGTGACGGCGACGCTGGTGCTCCTGGTGGTCTCCCTGGTCGAAGGG
CGCGAGCAGGGGTGGCCGGCGTGGAGCTTCGGCTGCCTGGCTCTCGCGGCGGTACTGTCCGGCGCGTCCGTCCTCCACCT
GCGTCGCCGTGCCGCACGCGGCGCTGACCCGTTGATCGACCCGGCGTTGTTCCGGGGGCGCGGCTTCTCGGTAGGGCTGG
CCGCCATGGTGGCCTACTTCCTGGCGATGGGTTCCTTCTTCTTCCTGCTGGCCCTCTACCTGCAGCAGGGCCGAGGCCTG
TCGCCGCTGGAGTCCGGGCTGCTGTTCCTGGCACTGGGAGCGGGCTACTTCGGCTCGTCGTTGGCGTCGATGCAGCTTGT
CGCGCGGATGGGCCGGTGGCTCGTGGTGGCCGGTCCGTTGACGATCGCGGTCGGCTACACGCTGGTCGGGATCACGGTCT
GGGCGACCGGCTCGACCGGGAGCCCGGCGTGGCTGGTGCCGGCGTTGCTCGTTGCCGGGCTCGGCATGGGGATGACCACC
GGCCCGCTCACGAACGTCGTCCTGACCGGTGTAGCGCCCGAACACGCCGCCTCCGCGTCGGGAGCGGCCAACACCGCGCA
GGAGGGCGGTGCGGCGCTGGGCGTGGCGGTCGCTGGGGCGGTCTTCTACCCCGCCCTCGGCGTCGCGGCGCGCGCCACCG
ACTATCCGGACGCCTTCGACGTCGCGTTGATCCCGCTGGTTGCCTGCTGTCTCGTCGCCGCGACGCTGGCGCTGTTGCTG
CCCGGCCGCCGCGCGCAGTAG

Upstream 100 bases:

>100_bases
TAGGCGTCGAATTACCTCTGACGTAATTGACGAGCCGACGGCGAGGCGTGGAGGGTGTGGCGACGCAGACATTTTTCGGC
CAGCCTGTAGGAGATCGCCC

Downstream 100 bases:

>100_bases
CCCGCATCCCACATCCGCAGCTCGAGGCTCGAGCTCCGCGCCGGCCGCAAGGAGGAGACGCCGCCATGCCAGTGGTCACC
GTCCGGGACGGAACCGAACT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTSGTSTPTATGEALADPTTAAEATAWGTLAVLMVGTFITVLDYFIANVAAPSVQRDLNASSAQIQGVIVGYGVAFTAGM
ITGGRLGDVYGRRRMFALGLLLFTVASAACGVAPNAEALIVARVVQGTAAALMVPQVLGILGTVYTGPSRAQAFNVYGLV
IGLAGVFGQVIGGVLITVDVAGLDWRAVFLLNVPICLVALAYTYRVVPESRGSARTRLDLTGAALVTATLVLLVVSLVEG
REQGWPAWSFGCLALAAVLSGASVLHLRRRAARGADPLIDPALFRGRGFSVGLAAMVAYFLAMGSFFFLLALYLQQGRGL
SPLESGLLFLALGAGYFGSSLASMQLVARMGRWLVVAGPLTIAVGYTLVGITVWATGSTGSPAWLVPALLVAGLGMGMTT
GPLTNVVLTGVAPEHAASASGAANTAQEGGAALGVAVAGAVFYPALGVAARATDYPDAFDVALIPLVACCLVAATLALLL
PGRRAQ

Sequences:

>Translated_486_residues
MTSGTSTPTATGEALADPTTAAEATAWGTLAVLMVGTFITVLDYFIANVAAPSVQRDLNASSAQIQGVIVGYGVAFTAGM
ITGGRLGDVYGRRRMFALGLLLFTVASAACGVAPNAEALIVARVVQGTAAALMVPQVLGILGTVYTGPSRAQAFNVYGLV
IGLAGVFGQVIGGVLITVDVAGLDWRAVFLLNVPICLVALAYTYRVVPESRGSARTRLDLTGAALVTATLVLLVVSLVEG
REQGWPAWSFGCLALAAVLSGASVLHLRRRAARGADPLIDPALFRGRGFSVGLAAMVAYFLAMGSFFFLLALYLQQGRGL
SPLESGLLFLALGAGYFGSSLASMQLVARMGRWLVVAGPLTIAVGYTLVGITVWATGSTGSPAWLVPALLVAGLGMGMTT
GPLTNVVLTGVAPEHAASASGAANTAQEGGAALGVAVAGAVFYPALGVAARATDYPDAFDVALIPLVACCLVAATLALLL
PGRRAQ
>Mature_485_residues
TSGTSTPTATGEALADPTTAAEATAWGTLAVLMVGTFITVLDYFIANVAAPSVQRDLNASSAQIQGVIVGYGVAFTAGMI
TGGRLGDVYGRRRMFALGLLLFTVASAACGVAPNAEALIVARVVQGTAAALMVPQVLGILGTVYTGPSRAQAFNVYGLVI
GLAGVFGQVIGGVLITVDVAGLDWRAVFLLNVPICLVALAYTYRVVPESRGSARTRLDLTGAALVTATLVLLVVSLVEGR
EQGWPAWSFGCLALAAVLSGASVLHLRRRAARGADPLIDPALFRGRGFSVGLAAMVAYFLAMGSFFFLLALYLQQGRGLS
PLESGLLFLALGAGYFGSSLASMQLVARMGRWLVVAGPLTIAVGYTLVGITVWATGSTGSPAWLVPALLVAGLGMGMTTG
PLTNVVLTGVAPEHAASASGAANTAQEGGAALGVAVAGAVFYPALGVAARATDYPDAFDVALIPLVACCLVAATLALLLP
GRRAQ

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=415, Percent_Identity=30.8433734939759, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87081983, Length=422, Percent_Identity=26.7772511848341, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788710, Length=421, Percent_Identity=25.41567695962, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789042, Length=416, Percent_Identity=27.4038461538462, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788392, Length=425, Percent_Identity=27.0588235294118, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324954, Length=401, Percent_Identity=22.4438902743142, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49390; Mature: 49259

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGTSTPTATGEALADPTTAAEATAWGTLAVLMVGTFITVLDYFIANVAAPSVQRDLNA
CCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
SSAQIQGVIVGYGVAFTAGMITGGRLGDVYGRRRMFALGLLLFTVASAACGVAPNAEALI
CCHHEEEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VARVVQGTAAALMVPQVLGILGTVYTGPSRAQAFNVYGLVIGLAGVFGQVIGGVLITVDV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
AGLDWRAVFLLNVPICLVALAYTYRVVPESRGSARTRLDLTGAALVTATLVLLVVSLVEG
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHC
REQGWPAWSFGCLALAAVLSGASVLHLRRRAARGADPLIDPALFRGRGFSVGLAAMVAYF
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHH
LAMGSFFFLLALYLQQGRGLSPLESGLLFLALGAGYFGSSLASMQLVARMGRWLVVAGPL
HHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEECHH
TIAVGYTLVGITVWATGSTGSPAWLVPALLVAGLGMGMTTGPLTNVVLTGVAPEHAASAS
HHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHEECCCCHHHCCCC
GAANTAQEGGAALGVAVAGAVFYPALGVAARATDYPDAFDVALIPLVACCLVAATLALLL
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PGRRAQ
CCCCCC
>Mature Secondary Structure 
TSGTSTPTATGEALADPTTAAEATAWGTLAVLMVGTFITVLDYFIANVAAPSVQRDLNA
CCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
SSAQIQGVIVGYGVAFTAGMITGGRLGDVYGRRRMFALGLLLFTVASAACGVAPNAEALI
CCHHEEEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VARVVQGTAAALMVPQVLGILGTVYTGPSRAQAFNVYGLVIGLAGVFGQVIGGVLITVDV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
AGLDWRAVFLLNVPICLVALAYTYRVVPESRGSARTRLDLTGAALVTATLVLLVVSLVEG
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHC
REQGWPAWSFGCLALAAVLSGASVLHLRRRAARGADPLIDPALFRGRGFSVGLAAMVAYF
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHH
LAMGSFFFLLALYLQQGRGLSPLESGLLFLALGAGYFGSSLASMQLVARMGRWLVVAGPL
HHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEECHH
TIAVGYTLVGITVWATGSTGSPAWLVPALLVAGLGMGMTTGPLTNVVLTGVAPEHAASAS
HHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHEECCCCHHHCCCC
GAANTAQEGGAALGVAVAGAVFYPALGVAARATDYPDAFDVALIPLVACCLVAATLALLL
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PGRRAQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]