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The map label for this gene is ydeM [C]

Identifier: 158317474

GI number: 158317474

Start: 6954941

End: 6956185

Strand: Direct

Name: ydeM [C]

Synonym: Franean1_5726

Alternate gene names: 158317474

Gene position: 6954941-6956185 (Clockwise)

Preceding gene: 158317471

Following gene: 158317475

Centisome position: 77.43

GC content: 66.18

Gene sequence:

>1245_bases
GTGATCGGCCCCCTCGGCACCGCCTATGGAGATCCGTCGGACGAATGGCCCGCCAACGAGCTCGACTTCCACCGGCTGAC
CGCCGCGGGATGGCGGCCCACGCCCTTCCGCCAGATCGTCCTCAAGATCAATAGCAGATGCAATCTGTCCTGCACCTACT
GTTATGTCTACCACCAGGCCGACCAGAACTGGCGGCAGCAGCCCGTCACGATGAGCCCGGCGGTGGTGGAGGCCACGGCA
CGCAGGCTCGCCGCACACGCAAAGACGCACGAACTCCCATGGATGCAGATCATCCTGCACGGCGGTGAGCCGCTCCTGGC
CGGCCACGACCACCTCCGGAACGTCGCGGAGCGTCTGCTCGCCGCGGTCGGCACTGACACCGCCGTGGAGTTCGTCCTCC
AGACCAACGGCACTCTGATCGACGAGGTGTTCCTCGAGCTCTGCCAGGAGTTCCGCATCCAGGTCGGGGTCAGCGTCGAC
GGCGATCTGGCGGTCAACGACCGGCGCCGGACCCGTCGCAACGGGAGTGGGAGCCACGCCGAGATCGCCCGAGGCCTGCA
CCTGCTCACCTCGGAACGTTTCCGGTCGATCTTCTCCGGGCTGCTCTGTGTCATCGACCCCACGAGCGATCCGGGAGAGG
TGTACGAGAGCCTGCTGGCATGGCGGCCACCGAGCGTCGACTTCCTCCTTCCGCACGGGAACTGGACGGCCCGCCCACCG
GCCCGGGACAGCGACGAGACGTCAACGCCCTACGCCGACTGGCTCGGCATGGTGTTCGACCGTTGGTATCCGGCCCCCAG
CCACGAGACGGACATCCGCCTGTTTCTGGAGATCATCTCGCTGATCCTCGGCGGCCACAGCCGCATCGAGACGGTCGGTC
TCTCGCCCTCGTCCGTCGTCGTCGTCGAGACCGACGGCTCGATCGAACAGGTCGACGCGCTCAAGTCCGCCTATCACGGC
GCGGCATCCACCGGCCTCACCGTTACGCGCAATGACCTCGACGAGGCGCTGCGCCATCCAGGTATCCTTGCCCGGCAGAT
CGGCGCCGCCGCGCTCGGCCGCGAGTGCACGCGATGCCCGGTGCACCGCGTGTGCGGAGGTGGATATTATCCCCACCGGT
ATCAGGCCGGCAGCGGCTTTCGGAACAGATCCGTCTACTGCCCGGATCTGTTCGCACTGATCACACGAATCGGCAAACAG
ATCCGGGCAGATATCGAAAAGGCAAAGCCGCAGGGTGAACATTGA

Upstream 100 bases:

>100_bases
CTCTTCAGTCGGCGGAGGAATCCGCAGAATTCCAACAGCCGCCGGCAGGGTTCCAATCGTCTCTCACCCCGACCTTCTGA
CGCCGGAACGGCGAGATCGT

Downstream 100 bases:

>100_bases
ATTCCATCGGCTCGCGCTGGCCGACTTCGACGACCTCGCGGCCGGTGGCGGGCGCACGGGAATCGTGCTCGGCCTGCGGA
GAACGCAGCTGAGCAAGCGG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MIGPLGTAYGDPSDEWPANELDFHRLTAAGWRPTPFRQIVLKINSRCNLSCTYCYVYHQADQNWRQQPVTMSPAVVEATA
RRLAAHAKTHELPWMQIILHGGEPLLAGHDHLRNVAERLLAAVGTDTAVEFVLQTNGTLIDEVFLELCQEFRIQVGVSVD
GDLAVNDRRRTRRNGSGSHAEIARGLHLLTSERFRSIFSGLLCVIDPTSDPGEVYESLLAWRPPSVDFLLPHGNWTARPP
ARDSDETSTPYADWLGMVFDRWYPAPSHETDIRLFLEIISLILGGHSRIETVGLSPSSVVVVETDGSIEQVDALKSAYHG
AASTGLTVTRNDLDEALRHPGILARQIGAAALGRECTRCPVHRVCGGGYYPHRYQAGSGFRNRSVYCPDLFALITRIGKQ
IRADIEKAKPQGEH

Sequences:

>Translated_414_residues
MIGPLGTAYGDPSDEWPANELDFHRLTAAGWRPTPFRQIVLKINSRCNLSCTYCYVYHQADQNWRQQPVTMSPAVVEATA
RRLAAHAKTHELPWMQIILHGGEPLLAGHDHLRNVAERLLAAVGTDTAVEFVLQTNGTLIDEVFLELCQEFRIQVGVSVD
GDLAVNDRRRTRRNGSGSHAEIARGLHLLTSERFRSIFSGLLCVIDPTSDPGEVYESLLAWRPPSVDFLLPHGNWTARPP
ARDSDETSTPYADWLGMVFDRWYPAPSHETDIRLFLEIISLILGGHSRIETVGLSPSSVVVVETDGSIEQVDALKSAYHG
AASTGLTVTRNDLDEALRHPGILARQIGAAALGRECTRCPVHRVCGGGYYPHRYQAGSGFRNRSVYCPDLFALITRIGKQ
IRADIEKAKPQGEH
>Mature_414_residues
MIGPLGTAYGDPSDEWPANELDFHRLTAAGWRPTPFRQIVLKINSRCNLSCTYCYVYHQADQNWRQQPVTMSPAVVEATA
RRLAAHAKTHELPWMQIILHGGEPLLAGHDHLRNVAERLLAAVGTDTAVEFVLQTNGTLIDEVFLELCQEFRIQVGVSVD
GDLAVNDRRRTRRNGSGSHAEIARGLHLLTSERFRSIFSGLLCVIDPTSDPGEVYESLLAWRPPSVDFLLPHGNWTARPP
ARDSDETSTPYADWLGMVFDRWYPAPSHETDIRLFLEIISLILGGHSRIETVGLSPSSVVVVETDGSIEQVDALKSAYHG
AASTGLTVTRNDLDEALRHPGILARQIGAAALGRECTRCPVHRVCGGGYYPHRYQAGSGFRNRSVYCPDLFALITRIGKQ
IRADIEKAKPQGEH

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine ('Cys-51' in the arylsulfatase CPF_0221) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical c

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=381, Percent_Identity=25.1968503937008, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994973, Length=350, Percent_Identity=23.7142857142857, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 45856; Mature: 45856

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGPLGTAYGDPSDEWPANELDFHRLTAAGWRPTPFRQIVLKINSRCNLSCTYCYVYHQA
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECC
DQNWRQQPVTMSPAVVEATARRLAAHAKTHELPWMQIILHGGEPLLAGHDHLRNVAERLL
CCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
AAVGTDTAVEFVLQTNGTLIDEVFLELCQEFRIQVGVSVDGDLAVNDRRRTRRNGSGSHA
HHHCCCHHEEEEECCCCCHHHHHHHHHHHHHCEEECCCCCCCEEECCHHHHCCCCCCCHH
EIARGLHLLTSERFRSIFSGLLCVIDPTSDPGEVYESLLAWRPPSVDFLLPHGNWTARPP
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
ARDSDETSTPYADWLGMVFDRWYPAPSHETDIRLFLEIISLILGGHSRIETVGLSPSSVV
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEE
VVETDGSIEQVDALKSAYHGAASTGLTVTRNDLDEALRHPGILARQIGAAALGRECTRCP
EEECCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCC
VHRVCGGGYYPHRYQAGSGFRNRSVYCPDLFALITRIGKQIRADIEKAKPQGEH
HHHHHCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIGPLGTAYGDPSDEWPANELDFHRLTAAGWRPTPFRQIVLKINSRCNLSCTYCYVYHQA
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECC
DQNWRQQPVTMSPAVVEATARRLAAHAKTHELPWMQIILHGGEPLLAGHDHLRNVAERLL
CCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
AAVGTDTAVEFVLQTNGTLIDEVFLELCQEFRIQVGVSVDGDLAVNDRRRTRRNGSGSHA
HHHCCCHHEEEEECCCCCHHHHHHHHHHHHHCEEECCCCCCCEEECCHHHHCCCCCCCHH
EIARGLHLLTSERFRSIFSGLLCVIDPTSDPGEVYESLLAWRPPSVDFLLPHGNWTARPP
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
ARDSDETSTPYADWLGMVFDRWYPAPSHETDIRLFLEIISLILGGHSRIETVGLSPSSVV
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEE
VVETDGSIEQVDALKSAYHGAASTGLTVTRNDLDEALRHPGILARQIGAAALGRECTRCP
EEECCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCC
VHRVCGGGYYPHRYQAGSGFRNRSVYCPDLFALITRIGKQIRADIEKAKPQGEH
HHHHHCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA