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The map label for this gene is thiC [H]

Identifier: 158317358

GI number: 158317358

Start: 6805135

End: 6806955

Strand: Direct

Name: thiC [H]

Synonym: Franean1_5609

Alternate gene names: 158317358

Gene position: 6805135-6806955 (Clockwise)

Preceding gene: 158317356

Following gene: 158317366

Centisome position: 75.76

GC content: 71.22

Gene sequence:

>1821_bases
GTGGTGCGTCAGATCCGCCCGACGGATTCCGACAACGCGGCGGCCGCGGCCTCCGGCACGGACGACGGCGCGGAGGGCAT
CCTGGCCCGGCCGTTCCCCGGGAGCAGGAAGACCTACCTGGTCGGGTCCCGGCCCGACCTGCGGGTGCCGATGCGCGAGG
TCGCGCTGAGCACGGGCGACAGCCTGGTCCTCTACGACACCTCCGGGCCGTACACCGACCCGGGCGCCGGCGTCGACGTG
CGGCGCGGCCTGCCCGCGCTGCGGGCCGGCTGGATCGCCGCCCGCGGCGACACCGAGGAGATCGAGGGGCGTGCCGTCCA
GCCCGCCGACGACGGGCGGCGCGGGCCTGACGGGCAGGCCGGTGACACCTTTCTCGGCTCGGGGCAGGCTCCGCGGCGGG
CTACCGCCGGCCGCGCGGTGACCCAGCTCGCCTACGCCCGGCGGGGCGAGATCACCGCCGAGATGGAGTTCGTCGCGCTG
CGTGAGGGCCTCTCTCCCGAGCTGGTGCGCGACGAGATCGCCGCGGGCCGGGCGGTGCTGCCGGCGAACGTGAACCACCC
CGAGAGCGAGCCGATGGCCATCGGCCGCAACCTGCTGGTGAAGATCAACGCGAACATCGGCAACTCCGCCGTCGCCTCCT
CCATCGAGGAGGAGGTCGAGAAGATGGTGTGGTCGACCCGCTGGGGCGCCGACACCGTCATGGACCTCTCGACCGGGCGG
AACATCCACACCACCCGCGAGTGGATCATCCGGAACTCCCCCGTCCCGATCGGGACGGTGCCGATCTACCAGGCGCTGGA
GAAGGTCGGCGGCAAGGCCGAGGAGCTGACCTGGGAGGTCTACCGGGACACGGTGATCGAGCAGGCCGAGCAGGGTGTGG
ACTACATGACCGTCCACGCCGGGGTGCTGCTGCGCTACGTGCCGATGACCGCGCGGCGCAAGACCGGCATCGTCTCGCGC
GGCGGCTCGATCCTGGCGGCATGGTGCCTCGCCCATCATGAGGAGAACTTCCTCTACACGAACTTCGCCGAGCTCTGCGA
GATCCTGCGGGCCTACGACGTCACCTTCTCCCTCGGCGACGGTCTGCGCCCCGGCTCCATCTCCGACGCGAACGACGCCG
CGCAGCTCGCCGAGCTCGCAACACTGGGCGAGCTGACCTCGGTGGCCTGGGAGCACGACGTCCAGGTGATGATCGAGGGG
CCGGGCCACGTGCCCATGCACAAGATCAAGGAGAACGTGGACCTGCAGCGCGAGCTGTGCCACGACGCGCCGTTCTACAC
CCTCGGCCCGCTCACCACCGACATCGCGCCCGGCTACGACCACATCACCTCGGCCATCGGCGCCGCGATGATCGGCTGGT
ACGGGACGGCGATGCTCTGCTACGTCACGCCCAAGGAGCACCTGGGCCTGCCGGACCGCGAGGACGTCAAGGCCGGGGTC
ATCGCCTACAAGATCGCGGCGCACGCCGCCGACCTGGCGAAGGGGCACCCGGGCTCCCAGGCCTGGGACGACGCCCTCTC
CGACGCGCGGTTCGACTTCCGCTGGGACGACCAGTTCAACCTCTCACTCGACCCGGAGACCGCCCGGGAGTACCACGACG
AGACACTGCCCGCGGCGCCCGCCAAGTCCGCGCACTTCTGCTCGATGTGCGGCCCGCACTTCTGCTCGATGCGGATCACG
CAGGACGTCCGCAAGTACGCGGCCGACCACGGCCTCGACACCGACGAGGCCGTCCAGGCCGGATTGGCGGAGAAGTCCCG
GGAGTTCGCCGAGCAGGGTGCCCGGATCTACCTGCCGCTCGCCGACCGGCAGACCCCCTGA

Upstream 100 bases:

>100_bases
GAGCTTCCACCCTGGGAGGCTGAGAGGGCGGCCGGGCAACTGCCGGTGCCGCCGACCGCCTGAACCTGTCCGGGTAATTC
CGGCGTAGGGAGTGATCAAG

Downstream 100 bases:

>100_bases
CCCGGCGGCCGCGGGCCTCCCCGACCGCCATGGCCGGGAGAGTCCCGCGGCCGGCTTCAGCTCTCCTTGCGGGCCAGCAC
GCGGTAGGTGTTGGAACGCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MVRQIRPTDSDNAAAAASGTDDGAEGILARPFPGSRKTYLVGSRPDLRVPMREVALSTGDSLVLYDTSGPYTDPGAGVDV
RRGLPALRAGWIAARGDTEEIEGRAVQPADDGRRGPDGQAGDTFLGSGQAPRRATAGRAVTQLAYARRGEITAEMEFVAL
REGLSPELVRDEIAAGRAVLPANVNHPESEPMAIGRNLLVKINANIGNSAVASSIEEEVEKMVWSTRWGADTVMDLSTGR
NIHTTREWIIRNSPVPIGTVPIYQALEKVGGKAEELTWEVYRDTVIEQAEQGVDYMTVHAGVLLRYVPMTARRKTGIVSR
GGSILAAWCLAHHEENFLYTNFAELCEILRAYDVTFSLGDGLRPGSISDANDAAQLAELATLGELTSVAWEHDVQVMIEG
PGHVPMHKIKENVDLQRELCHDAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDREDVKAGV
IAYKIAAHAADLAKGHPGSQAWDDALSDARFDFRWDDQFNLSLDPETAREYHDETLPAAPAKSAHFCSMCGPHFCSMRIT
QDVRKYAADHGLDTDEAVQAGLAEKSREFAEQGARIYLPLADRQTP

Sequences:

>Translated_606_residues
MVRQIRPTDSDNAAAAASGTDDGAEGILARPFPGSRKTYLVGSRPDLRVPMREVALSTGDSLVLYDTSGPYTDPGAGVDV
RRGLPALRAGWIAARGDTEEIEGRAVQPADDGRRGPDGQAGDTFLGSGQAPRRATAGRAVTQLAYARRGEITAEMEFVAL
REGLSPELVRDEIAAGRAVLPANVNHPESEPMAIGRNLLVKINANIGNSAVASSIEEEVEKMVWSTRWGADTVMDLSTGR
NIHTTREWIIRNSPVPIGTVPIYQALEKVGGKAEELTWEVYRDTVIEQAEQGVDYMTVHAGVLLRYVPMTARRKTGIVSR
GGSILAAWCLAHHEENFLYTNFAELCEILRAYDVTFSLGDGLRPGSISDANDAAQLAELATLGELTSVAWEHDVQVMIEG
PGHVPMHKIKENVDLQRELCHDAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDREDVKAGV
IAYKIAAHAADLAKGHPGSQAWDDALSDARFDFRWDDQFNLSLDPETAREYHDETLPAAPAKSAHFCSMCGPHFCSMRIT
QDVRKYAADHGLDTDEAVQAGLAEKSREFAEQGARIYLPLADRQTP
>Mature_606_residues
MVRQIRPTDSDNAAAAASGTDDGAEGILARPFPGSRKTYLVGSRPDLRVPMREVALSTGDSLVLYDTSGPYTDPGAGVDV
RRGLPALRAGWIAARGDTEEIEGRAVQPADDGRRGPDGQAGDTFLGSGQAPRRATAGRAVTQLAYARRGEITAEMEFVAL
REGLSPELVRDEIAAGRAVLPANVNHPESEPMAIGRNLLVKINANIGNSAVASSIEEEVEKMVWSTRWGADTVMDLSTGR
NIHTTREWIIRNSPVPIGTVPIYQALEKVGGKAEELTWEVYRDTVIEQAEQGVDYMTVHAGVLLRYVPMTARRKTGIVSR
GGSILAAWCLAHHEENFLYTNFAELCEILRAYDVTFSLGDGLRPGSISDANDAAQLAELATLGELTSVAWEHDVQVMIEG
PGHVPMHKIKENVDLQRELCHDAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDREDVKAGV
IAYKIAAHAADLAKGHPGSQAWDDALSDARFDFRWDDQFNLSLDPETAREYHDETLPAAPAKSAHFCSMCGPHFCSMRIT
QDVRKYAADHGLDTDEAVQAGLAEKSREFAEQGARIYLPLADRQTP

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=608, Percent_Identity=64.4736842105263, Blast_Score=807, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 65780; Mature: 65780

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRQIRPTDSDNAAAAASGTDDGAEGILARPFPGSRKTYLVGSRPDLRVPMREVALSTGD
CCCCCCCCCCCCCCEECCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCCHHHHHHCCCC
SLVLYDTSGPYTDPGAGVDVRRGLPALRAGWIAARGDTEEIEGRAVQPADDGRRGPDGQA
EEEEEECCCCCCCCCCCCHHHHCCCHHHHCEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
GDTFLGSGQAPRRATAGRAVTQLAYARRGEITAEMEFVALREGLSPELVRDEIAAGRAVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEE
PANVNHPESEPMAIGRNLLVKINANIGNSAVASSIEEEVEKMVWSTRWGADTVMDLSTGR
CCCCCCCCCCCHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
NIHTTREWIIRNSPVPIGTVPIYQALEKVGGKAEELTWEVYRDTVIEQAEQGVDYMTVHA
CCCCCHHHHEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHH
GVLLRYVPMTARRKTGIVSRGGSILAAWCLAHHEENFLYTNFAELCEILRAYDVTFSLGD
HHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCEEECCC
GLRPGSISDANDAAQLAELATLGELTSVAWEHDVQVMIEGPGHVPMHKIKENVDLQRELC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCHHHHHH
HDAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDREDVKAGV
HCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHHHHH
IAYKIAAHAADLAKGHPGSQAWDDALSDARFDFRWDDQFNLSLDPETAREYHDETLPAAP
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCEEEEECHHHHHHHHHHCCCCCC
AKSAHFCSMCGPHFCSMRITQDVRKYAADHGLDTDEAVQAGLAEKSREFAEQGARIYLPL
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
ADRQTP
CCCCCC
>Mature Secondary Structure
MVRQIRPTDSDNAAAAASGTDDGAEGILARPFPGSRKTYLVGSRPDLRVPMREVALSTGD
CCCCCCCCCCCCCCEECCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCCHHHHHHCCCC
SLVLYDTSGPYTDPGAGVDVRRGLPALRAGWIAARGDTEEIEGRAVQPADDGRRGPDGQA
EEEEEECCCCCCCCCCCCHHHHCCCHHHHCEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
GDTFLGSGQAPRRATAGRAVTQLAYARRGEITAEMEFVALREGLSPELVRDEIAAGRAVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEE
PANVNHPESEPMAIGRNLLVKINANIGNSAVASSIEEEVEKMVWSTRWGADTVMDLSTGR
CCCCCCCCCCCHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
NIHTTREWIIRNSPVPIGTVPIYQALEKVGGKAEELTWEVYRDTVIEQAEQGVDYMTVHA
CCCCCHHHHEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHH
GVLLRYVPMTARRKTGIVSRGGSILAAWCLAHHEENFLYTNFAELCEILRAYDVTFSLGD
HHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCEEECCC
GLRPGSISDANDAAQLAELATLGELTSVAWEHDVQVMIEGPGHVPMHKIKENVDLQRELC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCHHHHHH
HDAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDREDVKAGV
HCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHHHHH
IAYKIAAHAADLAKGHPGSQAWDDALSDARFDFRWDDQFNLSLDPETAREYHDETLPAAP
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCEEEEECHHHHHHHHHHCCCCCC
AKSAHFCSMCGPHFCSMRITQDVRKYAADHGLDTDEAVQAGLAEKSREFAEQGARIYLPL
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
ADRQTP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA