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The map label for this gene is polA [H]

Identifier: 158316934

GI number: 158316934

Start: 6213861

End: 6215360

Strand: Direct

Name: polA [H]

Synonym: Franean1_5178

Alternate gene names: 158316934

Gene position: 6213861-6215360 (Clockwise)

Preceding gene: 158316933

Following gene: 158316935

Centisome position: 69.18

GC content: 75.93

Gene sequence:

>1500_bases
GTGCGATTGCTCGGCCGACTGGGCTGCCAGGCCGGTCATTCACACGTACCATCGCCGATCGTGACGTCGACGGTTCCTGA
CTCAACGCACCAGGTCAGTGAGGGTGCGGCGATCGATGCTGCCGCCGCTGTCGAAGAGTCGGCAACTGTACCCATCCCGC
TGCTGGAGCCGGCCGGTGGCGTCCCGCCCGTCCTCGACGACCCGCAGGACCTCACCCGCGCCACCGAGCGGCTGGCCGCC
GGCACCGGCCCGGTCGCCTTCGACGCCGAGCGCGCCTCCGGTTACCGGTACAGCCAGCGTGCCTACCTCGTCCAGATCAG
GCGGGCCGGCGCGGGCACCCTGCTCATCGACCCGATCGCCCTGCCCGACCTGAGCGGCGTGCAGGACGCCGTCGGCGACA
CGGAATGGGTGCTGCACGCCGCCAGCCAGGACCTGCCCTGCCTGGCCGAGCTGGGCCTGCGCCCGAAGACGCTGTTCGAC
ACCGAGCTCGCCGGCCGTCTGCTGGGCTACGAACGGGTGGGGCTGGGCGCCATGGTCGAGCGGGTGCTCGGCTTCGGGTT
GGAGAAGGGGCACTCCGCCGCCGACTGGTCGAACCGCCCGCTGCCCGAACCGTGGCTGCGCTACGCCGCTCTCGACGTGG
AGCTGCTGGTGGAGCTGCGCGACCAGCTCGAGAGCGAGCTGGTCGCGCAGAACAAGATGGCGTTCGCCCGGCAGGAGTTC
GACGCGATCGCCGCCGCTCCGCCGCGGGAGCCGCGGGCCGAGCCGTGGCGGCGCACGAGCGGGATCCACCGCGCGCGCAG
CCGCCGCCAGCTCGCCGCCGTCCGGTCGATGTGGCAGGCCCGAGACCGGGTCGCCCGCACCCGCGACATCGCGCCGGGCC
GGGTGCTGCCCGACATCGCGATCATGGACGCGGTACTGAAGGCCCCCGAGGACGCCGCCGCGCTGACCCGGCTGCCGATC
TTCTCCGGGCCGCGGCTGCGGCGCTCGGCGACCACCTGGATGGATGCCCTGCGCGCCGCCGCCGAGATGCCCGAGAACCA
GCTGCCGAGCACCGCGGGCCCGGGCGGCGACGGCCTCCCCCCGCAGAACCGCTGGGCGGAACGTGACCCCGTGGCGGCGG
CGCGGCTGGCCCGCGTGCGCGCGGCGCTGAACGCGATCGCCGAGGATCAGGGCATGCCGGTGGAGAACCTGCTGGAGCCG
GCACTCGCCCGCCGGGTCACCTGGACTCCCCCGGCCACACGGGACACCGAGACGGTGACCGCCGCGCTGACCACGGGCGG
CGCCCGCCCCTGGCAGGTCGAGCTGACGGCGGCCGCGGTGGCCGCGGCCCTGGTGGACCCGCCCGCGGGCACCGCCGCCG
CGCCCGGAGCCACCGGCGGCGAGACCGCCGGCGGCGGAGCGGGCGGCACCGGTTCCGCGGCGGGCGGCGGGTCCGCGCGG
GCGAGAACGCGGCGGACCGGCGGCCGTCCGCTGTCCGGTGGGGAACCCGCCGCGTCCTGA

Upstream 100 bases:

>100_bases
TCCGGACCTCCCCGTCCAAGCACTGCGCGCCGGGAATGTCAACGGAACGCATCTGCAACGACTACCTTGGAGCCACCATC
GAACACGAAGCGTATAATTA

Downstream 100 bases:

>100_bases
TCCGGCCGCACGCGCGGTACGTACCGACCCTTACCTACCCATGGGTAACTCCTGCCGTAGGCTGTTCCCGTCGCGGTCCG
GCGCCACTGCGGGCAGCCGG

Product: 3'-5' exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MRLLGRLGCQAGHSHVPSPIVTSTVPDSTHQVSEGAAIDAAAAVEESATVPIPLLEPAGGVPPVLDDPQDLTRATERLAA
GTGPVAFDAERASGYRYSQRAYLVQIRRAGAGTLLIDPIALPDLSGVQDAVGDTEWVLHAASQDLPCLAELGLRPKTLFD
TELAGRLLGYERVGLGAMVERVLGFGLEKGHSAADWSNRPLPEPWLRYAALDVELLVELRDQLESELVAQNKMAFARQEF
DAIAAAPPREPRAEPWRRTSGIHRARSRRQLAAVRSMWQARDRVARTRDIAPGRVLPDIAIMDAVLKAPEDAAALTRLPI
FSGPRLRRSATTWMDALRAAAEMPENQLPSTAGPGGDGLPPQNRWAERDPVAAARLARVRAALNAIAEDQGMPVENLLEP
ALARRVTWTPPATRDTETVTAALTTGGARPWQVELTAAAVAAALVDPPAGTAAAPGATGGETAGGGAGGTGSAAGGGSAR
ARTRRTGGRPLSGGEPAAS

Sequences:

>Translated_499_residues
MRLLGRLGCQAGHSHVPSPIVTSTVPDSTHQVSEGAAIDAAAAVEESATVPIPLLEPAGGVPPVLDDPQDLTRATERLAA
GTGPVAFDAERASGYRYSQRAYLVQIRRAGAGTLLIDPIALPDLSGVQDAVGDTEWVLHAASQDLPCLAELGLRPKTLFD
TELAGRLLGYERVGLGAMVERVLGFGLEKGHSAADWSNRPLPEPWLRYAALDVELLVELRDQLESELVAQNKMAFARQEF
DAIAAAPPREPRAEPWRRTSGIHRARSRRQLAAVRSMWQARDRVARTRDIAPGRVLPDIAIMDAVLKAPEDAAALTRLPI
FSGPRLRRSATTWMDALRAAAEMPENQLPSTAGPGGDGLPPQNRWAERDPVAAARLARVRAALNAIAEDQGMPVENLLEP
ALARRVTWTPPATRDTETVTAALTTGGARPWQVELTAAAVAAALVDPPAGTAAAPGATGGETAGGGAGGTGSAAGGGSAR
ARTRRTGGRPLSGGEPAAS
>Mature_499_residues
MRLLGRLGCQAGHSHVPSPIVTSTVPDSTHQVSEGAAIDAAAAVEESATVPIPLLEPAGGVPPVLDDPQDLTRATERLAA
GTGPVAFDAERASGYRYSQRAYLVQIRRAGAGTLLIDPIALPDLSGVQDAVGDTEWVLHAASQDLPCLAELGLRPKTLFD
TELAGRLLGYERVGLGAMVERVLGFGLEKGHSAADWSNRPLPEPWLRYAALDVELLVELRDQLESELVAQNKMAFARQEF
DAIAAAPPREPRAEPWRRTSGIHRARSRRQLAAVRSMWQARDRVARTRDIAPGRVLPDIAIMDAVLKAPEDAAALTRLPI
FSGPRLRRSATTWMDALRAAAEMPENQLPSTAGPGGDGLPPQNRWAERDPVAAARLARVRAALNAIAEDQGMPVENLLEP
ALARRVTWTPPATRDTETVTAALTTGGARPWQVELTAAAVAAALVDPPAGTAAAPGATGGETAGGGAGGTGSAAGGGSAR
ARTRRTGGRPLSGGEPAAS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=283, Percent_Identity=29.6819787985866, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324574, Length=309, Percent_Identity=26.8608414239482, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161078302, Length=249, Percent_Identity=24.8995983935743, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 52336; Mature: 52336

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLGRLGCQAGHSHVPSPIVTSTVPDSTHQVSEGAAIDAAAAVEESATVPIPLLEPAGG
CCCHHHHCCCCCCCCCCCCCEECCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCC
VPPVLDDPQDLTRATERLAAGTGPVAFDAERASGYRYSQRAYLVQIRRAGAGTLLIDPIA
CCCCCCCHHHHHHHHHHHHCCCCCCEECCHHHCCCEEHHHHEEEEEECCCCCEEEECCCC
LPDLSGVQDAVGDTEWVLHAASQDLPCLAELGLRPKTLFDTELAGRLLGYERVGLGAMVE
CCCCCCHHHHHCCHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVLGFGLEKGHSAADWSNRPLPEPWLRYAALDVELLVELRDQLESELVAQNKMAFARQEF
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAIAAAPPREPRAEPWRRTSGIHRARSRRQLAAVRSMWQARDRVARTRDIAPGRVLPDIA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
IMDAVLKAPEDAAALTRLPIFSGPRLRRSATTWMDALRAAAEMPENQLPSTAGPGGDGLP
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCC
PQNRWAERDPVAAARLARVRAALNAIAEDQGMPVENLLEPALARRVTWTPPATRDTETVT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEECCCCCCCCCHHEE
AALTTGGARPWQVELTAAAVAAALVDPPAGTAAAPGATGGETAGGGAGGTGSAAGGGSAR
EEEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ARTRRTGGRPLSGGEPAAS
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLLGRLGCQAGHSHVPSPIVTSTVPDSTHQVSEGAAIDAAAAVEESATVPIPLLEPAGG
CCCHHHHCCCCCCCCCCCCCEECCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCC
VPPVLDDPQDLTRATERLAAGTGPVAFDAERASGYRYSQRAYLVQIRRAGAGTLLIDPIA
CCCCCCCHHHHHHHHHHHHCCCCCCEECCHHHCCCEEHHHHEEEEEECCCCCEEEECCCC
LPDLSGVQDAVGDTEWVLHAASQDLPCLAELGLRPKTLFDTELAGRLLGYERVGLGAMVE
CCCCCCHHHHHCCHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVLGFGLEKGHSAADWSNRPLPEPWLRYAALDVELLVELRDQLESELVAQNKMAFARQEF
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAIAAAPPREPRAEPWRRTSGIHRARSRRQLAAVRSMWQARDRVARTRDIAPGRVLPDIA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
IMDAVLKAPEDAAALTRLPIFSGPRLRRSATTWMDALRAAAEMPENQLPSTAGPGGDGLP
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCC
PQNRWAERDPVAAARLARVRAALNAIAEDQGMPVENLLEPALARRVTWTPPATRDTETVT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEECCCCCCCCCHHEE
AALTTGGARPWQVELTAAAVAAALVDPPAGTAAAPGATGGETAGGGAGGTGSAAGGGSAR
EEEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ARTRRTGGRPLSGGEPAAS
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]