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The map label for this gene is tnpR [H]

Identifier: 158316529

GI number: 158316529

Start: 5690526

End: 5691095

Strand: Reverse

Name: tnpR [H]

Synonym: Franean1_4764

Alternate gene names: 158316529

Gene position: 5691095-5690526 (Counterclockwise)

Preceding gene: 158316530

Following gene: 158316526

Centisome position: 63.36

GC content: 68.95

Gene sequence:

>570_bases
ATGCGGGTCGGTTACGTCAGGGTCAGCAGCGTCGACCAGAACACGGTGCGGCAACTTGACGGGGTCGACGTCGAGCGGGT
CTTCACCGACAAGGCGTCCGGGAAGGACAGCGACCGGCCGAAGCTCGACGAGCTGCTCGCGTTCGTCCGCGACGGCGACA
CCGTGCTCGTGCACTCCATGGACCGCCTCGCCCGCAACCTCGACGACCTCCGCCGCCTCGTCCGGACCCTCACCGGCAAA
GGCGTCCGGGTCGAGTTCGTCAAAGAGAGCCTCGCCTTCACCGGCGAGGACTCACCCATGGCAATGCTGCTGCTCTCCGT
CATGGGAGCCTTCGCCGAGTTCGAACGCGCTTTGATCCTCGAACGCCAACGCGAAGGCATCGCCGCCGCCCGCCAGCGCG
GCGTCTACACCGGCCGCACACCCGCCCTCACCCCCGACCAGGCCGACCAGCTCCGTGAACGCGCCGCCGCCGGCGAGCGC
AAGTCCGACCTGGCCCGCGCGTTCGGCATCAGCCGCGAAACCGTCTACACCTACCTACGGACCACAGCCGTCGCAGGCGC
CACCAGCTGA

Upstream 100 bases:

>100_bases
AGACGTCAGGTTAGAGTCCTTCGGCGTGTTTCTTGACACCTGTCCGGCAGGTTCTTAAAGTCCAACCTGACATTTCCGGG
TGCGGAGGGGGCCGGGGCGG

Downstream 100 bases:

>100_bases
ACCGACCCGGCGACTCCCATACCGAGAATGATCAGTTTGGCCTGTCAGTTACGAAATGGCCATGGCGGCCCTCCCTTCCA
GGTGGAGGGCTCCTGCTCTC

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MRVGYVRVSSVDQNTVRQLDGVDVERVFTDKASGKDSDRPKLDELLAFVRDGDTVLVHSMDRLARNLDDLRRLVRTLTGK
GVRVEFVKESLAFTGEDSPMAMLLLSVMGAFAEFERALILERQREGIAAARQRGVYTGRTPALTPDQADQLRERAAAGER
KSDLARAFGISRETVYTYLRTTAVAGATS

Sequences:

>Translated_189_residues
MRVGYVRVSSVDQNTVRQLDGVDVERVFTDKASGKDSDRPKLDELLAFVRDGDTVLVHSMDRLARNLDDLRRLVRTLTGK
GVRVEFVKESLAFTGEDSPMAMLLLSVMGAFAEFERALILERQREGIAAARQRGVYTGRTPALTPDQADQLRERAAAGER
KSDLARAFGISRETVYTYLRTTAVAGATS
>Mature_189_residues
MRVGYVRVSSVDQNTVRQLDGVDVERVFTDKASGKDSDRPKLDELLAFVRDGDTVLVHSMDRLARNLDDLRRLVRTLTGK
GVRVEFVKESLAFTGEDSPMAMLLLSVMGAFAEFERALILERQREGIAAARQRGVYTGRTPALTPDQADQLRERAAAGER
KSDLARAFGISRETVYTYLRTTAVAGATS

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=179, Percent_Identity=39.6648044692737, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787827, Length=182, Percent_Identity=36.8131868131868, Blast_Score=104, Evalue=3e-24,
Organism=Escherichia coli, GI1787638, Length=182, Percent_Identity=36.8131868131868, Blast_Score=103, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20939; Mature: 20939

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGYVRVSSVDQNTVRQLDGVDVERVFTDKASGKDSDRPKLDELLAFVRDGDTVLVHSM
CCCEEEEECCCCHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEHHHH
DRLARNLDDLRRLVRTLTGKGVRVEFVKESLAFTGEDSPMAMLLLSVMGAFAEFERALIL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERQREGIAAARQRGVYTGRTPALTPDQADQLRERAAAGERKSDLARAFGISRETVYTYLR
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHH
TTAVAGATS
HHHHCCCCC
>Mature Secondary Structure
MRVGYVRVSSVDQNTVRQLDGVDVERVFTDKASGKDSDRPKLDELLAFVRDGDTVLVHSM
CCCEEEEECCCCHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEHHHH
DRLARNLDDLRRLVRTLTGKGVRVEFVKESLAFTGEDSPMAMLLLSVMGAFAEFERALIL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERQREGIAAARQRGVYTGRTPALTPDQADQLRERAAAGERKSDLARAFGISRETVYTYLR
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHH
TTAVAGATS
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6312271 [H]