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The map label for this gene is tobE [H]

Identifier: 158316442

GI number: 158316442

Start: 5586323

End: 5587366

Strand: Reverse

Name: tobE [H]

Synonym: Franean1_4677

Alternate gene names: 158316442

Gene position: 5587366-5586323 (Counterclockwise)

Preceding gene: 158316443

Following gene: 158316441

Centisome position: 62.21

GC content: 78.83

Gene sequence:

>1044_bases
GTGACCGGACGCGCGGTGTGGATCGACGCGCCGGGCCGGCTGGAGATCCGCCCGGCCGAACCGGTCGCTCCCGGGCCGGG
TGAGGTGCTGGTCCGGGTGGCCCGGGCGGGTATCTGCGGCTCCGACCGCGAGGTGCTGCGCGGCACTCGGCCCGCCGGCT
TCGTCCGGTACCCGGTCGTCCCGGGGCACGAGTGGTCCGGGACGGTGGCGGCGGTCGGCCCGGCGGGGGACGCGGCGCTC
GTGGGCCGGCCGGTGGTCGCCGAGGGCTTCCGCTCCTGCCGGGTCTGTCCCGCCTGCCGGCGCGGCGACACCAACCTGTG
CACCGCCCCCTACGACGAGACCGGCTTCACCTGCCCCGGCGCGTGGTCCGACCACCTGGTCGTGCCGAGCCATCTCCTGC
ACGTCCTACCGGCCGGCGCCGACCTGCGCGCGGCCGCCGGCCTGGAACCGGCAGCCTGCGCGGCGGCCATGTGCCTGCGC
GCCGGTGCCGTTCCCGGCGAGCGGGTCGCCGTGGTCGGGGCGGGCACGCTCGGCCTGCTGGCCACACAGCTGCTGGCGGC
GGGCGGCCCGGCCGAGCTGGTCGCCGTGGACCCCCGCGCGGGCCGCGCCGAGCTCGCCGCCCGGTGCGGCGCCACGACGT
TGCTGTCACCGGACGAGGCCGCCGGCGGCCGGTGGTCACGGCGCTTCGACGTGGTGGTCGAGGCCGCCGGCGCGCCCGGC
AGCGCGGCGCTGGCCTGCGGGCTCGCCCGCCGTGGCGGGCGGGTGGTGCTCGCCGGTCTCGCCGCGGACGGCGACCACCC
GCTGCGCGCCCTCGACCTCGTGCTTCCCCAGCTCACCGTGCACACCGTGTTCGGCGCGCCGTCGCGAGCCTGGACCCACG
CGGTACGCGCGTTCGCCGCCGGCCAGCTCGACCCGGCGGCGCTGGTCACGCACGAGCTGCTCCTCGACGACGTCGGCGAG
GCGTTCCGGCTCCTCGACGACGCCGCCGGCACCGCCGTGAAGATCCTGCTGCGTCCCGCGTCCCGGAGCGGCACCGCCTC
GTGA

Upstream 100 bases:

>100_bases
TCGACGTCGTGGCCGCCCACCCGTCCCGCGGCGCCCACTTCGACCTCTACGCCGACGGCTGGGAGCTGCGCGGCTCCCGC
CCGCCCGGCCGCGGCTGACC

Downstream 100 bases:

>100_bases
CACCCCGCCTCGTCAACGGAAGGATGGCCGTGCCCACCGTCACGACCGACACCGAAGCGCTGCTCGCCGCCGCGGCGACG
GCGGCCCGGCCGTTCGGCGC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTGRAVWIDAPGRLEIRPAEPVAPGPGEVLVRVARAGICGSDREVLRGTRPAGFVRYPVVPGHEWSGTVAAVGPAGDAAL
VGRPVVAEGFRSCRVCPACRRGDTNLCTAPYDETGFTCPGAWSDHLVVPSHLLHVLPAGADLRAAAGLEPAACAAAMCLR
AGAVPGERVAVVGAGTLGLLATQLLAAGGPAELVAVDPRAGRAELAARCGATTLLSPDEAAGGRWSRRFDVVVEAAGAPG
SAALACGLARRGGRVVLAGLAADGDHPLRALDLVLPQLTVHTVFGAPSRAWTHAVRAFAAGQLDPAALVTHELLLDDVGE
AFRLLDDAAGTAVKILLRPASRSGTAS

Sequences:

>Translated_347_residues
MTGRAVWIDAPGRLEIRPAEPVAPGPGEVLVRVARAGICGSDREVLRGTRPAGFVRYPVVPGHEWSGTVAAVGPAGDAAL
VGRPVVAEGFRSCRVCPACRRGDTNLCTAPYDETGFTCPGAWSDHLVVPSHLLHVLPAGADLRAAAGLEPAACAAAMCLR
AGAVPGERVAVVGAGTLGLLATQLLAAGGPAELVAVDPRAGRAELAARCGATTLLSPDEAAGGRWSRRFDVVVEAAGAPG
SAALACGLARRGGRVVLAGLAADGDHPLRALDLVLPQLTVHTVFGAPSRAWTHAVRAFAAGQLDPAALVTHELLLDDVGE
AFRLLDDAAGTAVKILLRPASRSGTAS
>Mature_346_residues
TGRAVWIDAPGRLEIRPAEPVAPGPGEVLVRVARAGICGSDREVLRGTRPAGFVRYPVVPGHEWSGTVAAVGPAGDAALV
GRPVVAEGFRSCRVCPACRRGDTNLCTAPYDETGFTCPGAWSDHLVVPSHLLHVLPAGADLRAAAGLEPAACAAAMCLRA
GAVPGERVAVVGAGTLGLLATQLLAAGGPAELVAVDPRAGRAELAARCGATTLLSPDEAAGGRWSRRFDVVVEAAGAPGS
AALACGLARRGGRVVLAGLAADGDHPLRALDLVLPQLTVHTVFGAPSRAWTHAVRAFAAGQLDPAALVTHELLLDDVGEA
FRLLDDAAGTAVKILLRPASRSGTAS

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=355, Percent_Identity=29.2957746478873, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI71743840, Length=368, Percent_Identity=26.3586956521739, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI262073058, Length=368, Percent_Identity=26.3586956521739, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI156523966, Length=356, Percent_Identity=25.8426966292135, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI4501939, Length=344, Percent_Identity=26.1627906976744, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4501929, Length=373, Percent_Identity=24.3967828418231, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI71565154, Length=369, Percent_Identity=23.8482384823848, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4501933, Length=362, Percent_Identity=25.1381215469613, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788073, Length=340, Percent_Identity=29.4117647058824, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787863, Length=339, Percent_Identity=28.3185840707965, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788075, Length=338, Percent_Identity=27.810650887574, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI226510992, Length=329, Percent_Identity=29.7872340425532, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788407, Length=342, Percent_Identity=28.9473684210526, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790045, Length=320, Percent_Identity=26.25, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790718, Length=318, Percent_Identity=27.9874213836478, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786552, Length=377, Percent_Identity=28.3819628647215, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082125, Length=359, Percent_Identity=25.0696378830084, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786518, Length=285, Percent_Identity=32.280701754386, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=339, Percent_Identity=28.6135693215339, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17562878, Length=345, Percent_Identity=28.9855072463768, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25146526, Length=349, Percent_Identity=26.647564469914, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562584, Length=329, Percent_Identity=26.4437689969605, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=358, Percent_Identity=25.4189944134078, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319955, Length=340, Percent_Identity=26.1764705882353, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=340, Percent_Identity=26.1764705882353, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319258, Length=356, Percent_Identity=25.8426966292135, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=372, Percent_Identity=24.1935483870968, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319621, Length=221, Percent_Identity=28.5067873303167, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319257, Length=375, Percent_Identity=25.0666666666667, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=217, Percent_Identity=27.6497695852535, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323961, Length=296, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323729, Length=291, Percent_Identity=23.7113402061856, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17737897, Length=350, Percent_Identity=30.2857142857143, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137530, Length=352, Percent_Identity=28.9772727272727, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17737895, Length=375, Percent_Identity=24.8, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: NA

Molecular weight: Translated: 35212; Mature: 35080

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRAVWIDAPGRLEIRPAEPVAPGPGEVLVRVARAGICGSDREVLRGTRPAGFVRYPVV
CCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECCC
PGHEWSGTVAAVGPAGDAALVGRPVVAEGFRSCRVCPACRRGDTNLCTAPYDETGFTCPG
CCCCCCCEEEEECCCCCCHHCCCCHHHHHHHHCCCCHHHCCCCCCEEECCCCCCCCCCCC
AWSDHLVVPSHLLHVLPAGADLRAAAGLEPAACAAAMCLRAGAVPGERVAVVGAGTLGLL
CCCCCEECHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHH
ATQLLAAGGPAELVAVDPRAGRAELAARCGATTLLSPDEAAGGRWSRRFDVVVEAAGAPG
HHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEECCCCCCCCCCCCEEEEEEEEECCCCC
SAALACGLARRGGRVVLAGLAADGDHPLRALDLVLPQLTVHTVFGAPSRAWTHAVRAFAA
HHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHC
GQLDPAALVTHELLLDDVGEAFRLLDDAAGTAVKILLRPASRSGTAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
TGRAVWIDAPGRLEIRPAEPVAPGPGEVLVRVARAGICGSDREVLRGTRPAGFVRYPVV
CCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECCC
PGHEWSGTVAAVGPAGDAALVGRPVVAEGFRSCRVCPACRRGDTNLCTAPYDETGFTCPG
CCCCCCCEEEEECCCCCCHHCCCCHHHHHHHHCCCCHHHCCCCCCEEECCCCCCCCCCCC
AWSDHLVVPSHLLHVLPAGADLRAAAGLEPAACAAAMCLRAGAVPGERVAVVGAGTLGLL
CCCCCEECHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHH
ATQLLAAGGPAELVAVDPRAGRAELAARCGATTLLSPDEAAGGRWSRRFDVVVEAAGAPG
HHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEECCCCCCCCCCCCEEEEEEEEECCCCC
SAALACGLARRGGRVVLAGLAADGDHPLRALDLVLPQLTVHTVFGAPSRAWTHAVRAFAA
HHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHC
GQLDPAALVTHELLLDDVGEAFRLLDDAAGTAVKILLRPASRSGTAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA