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The map label for this gene is fabI [H]

Identifier: 158316258

GI number: 158316258

Start: 5349549

End: 5350292

Strand: Reverse

Name: fabI [H]

Synonym: Franean1_4483

Alternate gene names: 158316258

Gene position: 5350292-5349549 (Counterclockwise)

Preceding gene: 158316259

Following gene: 158316256

Centisome position: 59.57

GC content: 72.45

Gene sequence:

>744_bases
ATGAGGACAGCGGTTGTGGTCGGCGCCGGCGGCATCGCCGCCGCCACCGCGATGGAACTGGCCGGGCGCGGGTACACGAC
GATCCATGTCCTGACCCGTGGGGAGTCGGACGCGGGCGTCAAGCTTCGCGGCGCACTCGACGAGCGCGGTGTCAGCGGGA
TCTCCGCGCACTGCGACGTGACGGACTGGACGTCCGTGGCAGCGGCGTGCGCGGCGGTGCCGGGACCCGTGCACGCCGTC
GTCTACACCGCGGGCGCGCGGGCGACGGCGACACCGGGCGAACTCACGCACGACACCTGGCGCCAGATGACCGACATCTA
CGCGGGCGGTCTGGTCGCGGTGCTCCAGGCGCTGGAGACGAAGCTGGCGTCCGGGTCGGCGGTGGTCGCGCTGTCCGGGA
CGTCGGGTCGGCGGGTGGTGTCGCCCGGGCACCTCGCGATGGGCTCGGCGAAAGCCGCGATGGACCAGTCCATCGGCTAC
CTCGCGCACTGGCTCGCACCCCGCGGGGTGCGGGTCAACGCGGTGTGCTGTGGACCGGTCGACACCCCGACCGTCCGGTC
CCACCTGGCGCCGGACGAGCTGAACCGGCTGTCCGCGGAGTACACGACCAGAACTCTCGCCGGCCGGCTGGCGCGTCCGG
ACGACGTGGCCGCGGCGGTCACGATGCTCTGCGGCGACGAGAGCCGCTGGATCTACGGACAGGTCATCCTTGCGGACGGC
GGCGAGGAACTGCTACTCGGTTGA

Upstream 100 bases:

>100_bases
GTGAGATGCGGATCCAGGGGACCGCGGCCTCGCTGTCGGCGAACAGAGCCTTGCTCGAATCCAGCTACCTCGGCGAGAGT
CAGGCAGGAGTACATCAGTC

Downstream 100 bases:

>100_bases
TCGCGGGAACCGGGAACCGTGAGGCCTGGACAGCGGGCGACCGGATGCCACCACCGGACGGGATCTCGGCCAGCGGGGTT
CAACCGAGATTTTCGGCCGG

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRTAVVVGAGGIAAATAMELAGRGYTTIHVLTRGESDAGVKLRGALDERGVSGISAHCDVTDWTSVAAACAAVPGPVHAV
VYTAGARATATPGELTHDTWRQMTDIYAGGLVAVLQALETKLASGSAVVALSGTSGRRVVSPGHLAMGSAKAAMDQSIGY
LAHWLAPRGVRVNAVCCGPVDTPTVRSHLAPDELNRLSAEYTTRTLAGRLARPDDVAAAVTMLCGDESRWIYGQVILADG
GEELLLG

Sequences:

>Translated_247_residues
MRTAVVVGAGGIAAATAMELAGRGYTTIHVLTRGESDAGVKLRGALDERGVSGISAHCDVTDWTSVAAACAAVPGPVHAV
VYTAGARATATPGELTHDTWRQMTDIYAGGLVAVLQALETKLASGSAVVALSGTSGRRVVSPGHLAMGSAKAAMDQSIGY
LAHWLAPRGVRVNAVCCGPVDTPTVRSHLAPDELNRLSAEYTTRTLAGRLARPDDVAAAVTMLCGDESRWIYGQVILADG
GEELLLG
>Mature_247_residues
MRTAVVVGAGGIAAATAMELAGRGYTTIHVLTRGESDAGVKLRGALDERGVSGISAHCDVTDWTSVAAACAAVPGPVHAV
VYTAGARATATPGELTHDTWRQMTDIYAGGLVAVLQALETKLASGSAVVALSGTSGRRVVSPGHLAMGSAKAAMDQSIGY
LAHWLAPRGVRVNAVCCGPVDTPTVRSHLAPDELNRLSAEYTTRTLAGRLARPDDVAAAVTMLCGDESRWIYGQVILADG
GEELLLG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=202, Percent_Identity=29.7029702970297, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787545, Length=212, Percent_Identity=28.7735849056604, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082100, Length=252, Percent_Identity=29.3650793650794, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24643142, Length=244, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 25330; Mature: 25330

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTAVVVGAGGIAAATAMELAGRGYTTIHVLTRGESDAGVKLRGALDERGVSGISAHCDV
CCEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCEECCCC
TDWTSVAAACAAVPGPVHAVVYTAGARATATPGELTHDTWRQMTDIYAGGLVAVLQALET
CHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
KLASGSAVVALSGTSGRRVVSPGHLAMGSAKAAMDQSIGYLAHWLAPRGVRVNAVCCGPV
HHCCCCEEEEEECCCCCEEECCCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DTPTVRSHLAPDELNRLSAEYTTRTLAGRLARPDDVAAAVTMLCGDESRWIYGQVILADG
CCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECC
GEELLLG
CCEECCC
>Mature Secondary Structure
MRTAVVVGAGGIAAATAMELAGRGYTTIHVLTRGESDAGVKLRGALDERGVSGISAHCDV
CCEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCEECCCC
TDWTSVAAACAAVPGPVHAVVYTAGARATATPGELTHDTWRQMTDIYAGGLVAVLQALET
CHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
KLASGSAVVALSGTSGRRVVSPGHLAMGSAKAAMDQSIGYLAHWLAPRGVRVNAVCCGPV
HHCCCCEEEEEECCCCCEEECCCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DTPTVRSHLAPDELNRLSAEYTTRTLAGRLARPDDVAAAVTMLCGDESRWIYGQVILADG
CCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECC
GEELLLG
CCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]