Click here to switch to the map view.

The map label for this gene is hrdB [H]

Identifier: 158316157

GI number: 158316157

Start: 5225702

End: 5226904

Strand: Reverse

Name: hrdB [H]

Synonym: Franean1_4379

Alternate gene names: 158316157

Gene position: 5226904-5225702 (Counterclockwise)

Preceding gene: 158316159

Following gene: 158316151

Centisome position: 58.19

GC content: 63.26

Gene sequence:

>1203_bases
ATGGGCCGGTCTTTCCGCGGACATGAAGATGCGCCGCCCACGGATCTGAGCGCCCCCGACGAAGCCGCGGAGGAAAAGAT
CCGATCCCTCATCGCCCGGGGTAAGGAGAACGGTTTCGTCACCCCGGACGACATTGCCGCTGCGCTACTCGCAGCGGAGC
TGCCGCCAGAGAGCAGCGACGTTGTCCTACGGCTACTCGCGGAGGACGGCATCGAGGTCCTCGACGAGGTGGGCGGGGAC
GCTTCAGACATGCCTAGCCGGCGTCGTGAGGGAGAGGAACTTGCGCTTACGACGCCACCCTCGGACCCGGTACGGATGTA
TCTCAAGGCCATCGGCCGGGTGCGGCTGCTGACCGCAGAGGAAGAGGTCGACCTAGCGAAGCGGATCGAGGCGGGTCTGT
TCGCCTCCGAGAAGCTCGCCGCCATCCGAAGGACCTCCCCGCGGCTGCGTCGGGACTTGGAGGCGATCGAGCAGGACGGT
CAGATCGCCAAGCGCAAACTGGTGGAGGCGAACCTGCGCCTCGTGGTGTCCATCGCTAAGCGGTACGTCGGCCGGGGCAT
GCTGCTGCTGGACCTGATCCAGGAGGGCAACCTGGGCCTGATCCGTGCGGTGGAGAAGTTCGACTACACCAAGGGATACA
AGTTCTCCACCTACGCCACCTGGTGGATCCGGCAGGCTGTCACGAGGGCCATCGCGGATCAGGGGCGCACCATCCGGATT
CCGGTACACATGGTCGAGACAATCAACAAGGTCACCCGGATCCAGCGGCAACTATTGCAGGATCTGGGCCGGGAGCCTTC
GCCGGAAGAGATCGCCACACAGGTAGACCTCGCACCGCACAGAGTGGAGGAAATTCTCAAAGTCGGACAGACACCGGTCG
CCCTGGAGACCCCGATCGGCGAGGAGCAGGACTCCCAGCTCGGAGACTTCATCGAGGACAACGACGCGATTGTGCCGTTC
GAAGCGGCAAGTTTCGTCCTCCTGCAGGAGCAAATCGACTCGGTCCTACACACGCTGTCCGAGCGGGAGAAGAAAGTCAT
CCAGCTCCGGTTCGGTCTGACTGACGGCCAGCCTAGGACGTTGGAGCAGGTAGGCCGGGAATTCGGGGTGACCCGGGAAC
GGATCCGGCAGATAGAATCAAGAACACTGGCGAAGCTAAGCCACCCGGCGCGTTCACAACGGCTACGCGACTACCTGGTA
TAG

Upstream 100 bases:

>100_bases
CTGCGTCCTCGCTGCGGGCCCGTTCCCAGTGGAGACTTAGAATATGGCTGGGTCTCCTGGAAACATCTGCCGCCGACGCT
GAGCATAGGTGCTGACTGAC

Downstream 100 bases:

>100_bases
CCCTGCTTCAAGCGAGACCGCAGTCACGGGGTCTGACGTCCGGCTAAGGGCGAGGGCCCGCGCTGGTGGCCGGCGTCATC
GACTGATGGGTCGAGGTGCA

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MGRSFRGHEDAPPTDLSAPDEAAEEKIRSLIARGKENGFVTPDDIAAALLAAELPPESSDVVLRLLAEDGIEVLDEVGGD
ASDMPSRRREGEELALTTPPSDPVRMYLKAIGRVRLLTAEEEVDLAKRIEAGLFASEKLAAIRRTSPRLRRDLEAIEQDG
QIAKRKLVEANLRLVVSIAKRYVGRGMLLLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAVTRAIADQGRTIRI
PVHMVETINKVTRIQRQLLQDLGREPSPEEIATQVDLAPHRVEEILKVGQTPVALETPIGEEQDSQLGDFIEDNDAIVPF
EAASFVLLQEQIDSVLHTLSEREKKVIQLRFGLTDGQPRTLEQVGREFGVTRERIRQIESRTLAKLSHPARSQRLRDYLV

Sequences:

>Translated_400_residues
MGRSFRGHEDAPPTDLSAPDEAAEEKIRSLIARGKENGFVTPDDIAAALLAAELPPESSDVVLRLLAEDGIEVLDEVGGD
ASDMPSRRREGEELALTTPPSDPVRMYLKAIGRVRLLTAEEEVDLAKRIEAGLFASEKLAAIRRTSPRLRRDLEAIEQDG
QIAKRKLVEANLRLVVSIAKRYVGRGMLLLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAVTRAIADQGRTIRI
PVHMVETINKVTRIQRQLLQDLGREPSPEEIATQVDLAPHRVEEILKVGQTPVALETPIGEEQDSQLGDFIEDNDAIVPF
EAASFVLLQEQIDSVLHTLSEREKKVIQLRFGLTDGQPRTLEQVGREFGVTRERIRQIESRTLAKLSHPARSQRLRDYLV
>Mature_399_residues
GRSFRGHEDAPPTDLSAPDEAAEEKIRSLIARGKENGFVTPDDIAAALLAAELPPESSDVVLRLLAEDGIEVLDEVGGDA
SDMPSRRREGEELALTTPPSDPVRMYLKAIGRVRLLTAEEEVDLAKRIEAGLFASEKLAAIRRTSPRLRRDLEAIEQDGQ
IAKRKLVEANLRLVVSIAKRYVGRGMLLLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAVTRAIADQGRTIRIP
VHMVETINKVTRIQRQLLQDLGREPSPEEIATQVDLAPHRVEEILKVGQTPVALETPIGEEQDSQLGDFIEDNDAIVPFE
AASFVLLQEQIDSVLHTLSEREKKVIQLRFGLTDGQPRTLEQVGREFGVTRERIRQIESRTLAKLSHPARSQRLRDYLV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=57.8059071729958, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789098, Length=331, Percent_Identity=40.785498489426, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1789871, Length=316, Percent_Identity=24.3670886075949, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 44829; Mature: 44698

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRSFRGHEDAPPTDLSAPDEAAEEKIRSLIARGKENGFVTPDDIAAALLAAELPPESSD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHH
VVLRLLAEDGIEVLDEVGGDASDMPSRRREGEELALTTPPSDPVRMYLKAIGRVRLLTAE
HHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHCCEEEEECH
EEVDLAKRIEAGLFASEKLAAIRRTSPRLRRDLEAIEQDGQIAKRKLVEANLRLVVSIAK
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
RYVGRGMLLLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAVTRAIADQGRTIRI
HHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
PVHMVETINKVTRIQRQLLQDLGREPSPEEIATQVDLAPHRVEEILKVGQTPVALETPIG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCC
EEQDSQLGDFIEDNDAIVPFEAASFVLLQEQIDSVLHTLSEREKKVIQLRFGLTDGQPRT
CCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHH
LEQVGREFGVTRERIRQIESRTLAKLSHPARSQRLRDYLV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
GRSFRGHEDAPPTDLSAPDEAAEEKIRSLIARGKENGFVTPDDIAAALLAAELPPESSD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHH
VVLRLLAEDGIEVLDEVGGDASDMPSRRREGEELALTTPPSDPVRMYLKAIGRVRLLTAE
HHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHCCEEEEECH
EEVDLAKRIEAGLFASEKLAAIRRTSPRLRRDLEAIEQDGQIAKRKLVEANLRLVVSIAK
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
RYVGRGMLLLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAVTRAIADQGRTIRI
HHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
PVHMVETINKVTRIQRQLLQDLGREPSPEEIATQVDLAPHRVEEILKVGQTPVALETPIG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCC
EEQDSQLGDFIEDNDAIVPFEAASFVLLQEQIDSVLHTLSEREKKVIQLRFGLTDGQPRT
CCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHH
LEQVGREFGVTRERIRQIESRTLAKLSHPARSQRLRDYLV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8690707 [H]