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The map label for this gene is yhjG [H]

Identifier: 158315918

GI number: 158315918

Start: 4915169

End: 4916608

Strand: Reverse

Name: yhjG [H]

Synonym: Franean1_4133

Alternate gene names: 158315918

Gene position: 4916608-4915169 (Counterclockwise)

Preceding gene: 158315919

Following gene: 158315916

Centisome position: 54.74

GC content: 68.61

Gene sequence:

>1440_bases
ATGATTGATGTGATAGTGGTGGGCGGCGGACCGACCGGCCTGATGCTCGCCAGTGAACTGCGACTGCATGGCATCGACGT
GCGGATCCTCGAGAAGGACACCGAGCCGACCCCGGTCGTCCGCTCCCTCGGCCTCCACGCCCGCAGCATCGAGATCATGG
ACCAGCGTGGCCTGCTCGACCGGTTCCTCGCCCTCGGCACCCAACACCCGGTCGGTGGCTTCTTCGCCGGCATCAGCAAA
CCCGCACCCGACCAGCTCGACACCGCACACCCCTACGTCCTCGGCATCCCACAGCCCACCACCGACCGCCTCCTCGCCGA
ACACGCCACCGCACTCGGCGCCGACATCCGCCGCGGCCACGAACTGGTCGGACTCAACCAAGACGACGACACGGTCACCG
CACACCTGGCCGACGGCACACACCTCCACGCCCGCTACCTCGTCGGCTGCGACGGCGGCCGCAGCACCGTCCGCAAACTG
CTCGGCGTCCCCTTCCCCGGCGAACCCACCCGACACCAGACGCTCCTCGCCGAAGCGGAACTGACCGCCCCACCGGAGAC
GATCAACGCCATAGTGACCAAGGTCCGCGAAACCCACAAACGGTTCGGCGCCGGCCCGCTGGGAAACGGGCTGTACCGCC
TCATCGCACCCGCCGACGGGGTCGTCGAGGACCGCACCCCACCCACCCTCGACGAACTCAAACAGCAGATACGCAGACTC
GCCGGCACCGACTTCGGCGCACACTCACCCCGCTGGCTCTCCCGCTTCGGTGACGCCACCCGCCAGGCCGAGCGGTACCG
CACCGGCCGGGTTCTGCTCGCCGGCGACGCCGCGCACATCCACCCGCCGACCGGAGGACAGGGACTCAACCTCGGCGTGC
AGGACGCGTTCAACCTCGGCTGGAAACTCGCCGCCACGATCAACGGCTGGGCACCGGCCGGACTGCTGGACACCTACCAC
ACCGAACGACACCCGGTCGCCGCCGACGTCCTCACCAACACCCGTGCGCAGATGGAGCTGATGTCCCTCGACCCCGGCGC
GCAGGCAGTACGCCGACTCCTGGTCGAACTGATGGACTTCGACGACGTCAACCGACACCTCACCGAAAAAATCATCGCGA
TCGGGATCCGCTACGACTTCGGCGCCGGCCATCATCTGCTCGGCCGGCGGATGCGCGACATCGCGCTCAAACGCGGCCGC
CTCTACCCGCTGATGCACCACGGCCGCGGACTGCTCCTCGACCAGACCGGACAGCTCTCCGTCACCGGCTGGGCGGACCG
GGTCGACCACATCATCGACGTCAGCGACGAACTCGACGTCCCCGCCGCGCTCCTACGCCCCGACGGCCACATCGCCTGGG
TCGGCGACAACCAGCAGGACCTGCTCGGCCACCTACCCACCTGGTTCGGCACCGCCACCACCCGAGCACAACGAAGCTGA

Upstream 100 bases:

>100_bases
CGCGAAGCATCCGCGGGTACGTAGCCGCGCGGCCGGACCACGCAAAAAACTGGCCGAACAGAACCCTCGGTGAAATGGCA
CTCTCGGTTAATGAGGATTC

Downstream 100 bases:

>100_bases
TCACCATCACAGCGGAGGCAGGTCAGCCGAGGGTCCGCCGGTACCGCACGAGCCTGTCCCTGGTGGAGCCATCCGGTTCC
CAACCCAGCTGGGAGTAGAA

Product: pentachlorophenol monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MIDVIVVGGGPTGLMLASELRLHGIDVRILEKDTEPTPVVRSLGLHARSIEIMDQRGLLDRFLALGTQHPVGGFFAGISK
PAPDQLDTAHPYVLGIPQPTTDRLLAEHATALGADIRRGHELVGLNQDDDTVTAHLADGTHLHARYLVGCDGGRSTVRKL
LGVPFPGEPTRHQTLLAEAELTAPPETINAIVTKVRETHKRFGAGPLGNGLYRLIAPADGVVEDRTPPTLDELKQQIRRL
AGTDFGAHSPRWLSRFGDATRQAERYRTGRVLLAGDAAHIHPPTGGQGLNLGVQDAFNLGWKLAATINGWAPAGLLDTYH
TERHPVAADVLTNTRAQMELMSLDPGAQAVRRLLVELMDFDDVNRHLTEKIIAIGIRYDFGAGHHLLGRRMRDIALKRGR
LYPLMHHGRGLLLDQTGQLSVTGWADRVDHIIDVSDELDVPAALLRPDGHIAWVGDNQQDLLGHLPTWFGTATTRAQRS

Sequences:

>Translated_479_residues
MIDVIVVGGGPTGLMLASELRLHGIDVRILEKDTEPTPVVRSLGLHARSIEIMDQRGLLDRFLALGTQHPVGGFFAGISK
PAPDQLDTAHPYVLGIPQPTTDRLLAEHATALGADIRRGHELVGLNQDDDTVTAHLADGTHLHARYLVGCDGGRSTVRKL
LGVPFPGEPTRHQTLLAEAELTAPPETINAIVTKVRETHKRFGAGPLGNGLYRLIAPADGVVEDRTPPTLDELKQQIRRL
AGTDFGAHSPRWLSRFGDATRQAERYRTGRVLLAGDAAHIHPPTGGQGLNLGVQDAFNLGWKLAATINGWAPAGLLDTYH
TERHPVAADVLTNTRAQMELMSLDPGAQAVRRLLVELMDFDDVNRHLTEKIIAIGIRYDFGAGHHLLGRRMRDIALKRGR
LYPLMHHGRGLLLDQTGQLSVTGWADRVDHIIDVSDELDVPAALLRPDGHIAWVGDNQQDLLGHLPTWFGTATTRAQRS
>Mature_479_residues
MIDVIVVGGGPTGLMLASELRLHGIDVRILEKDTEPTPVVRSLGLHARSIEIMDQRGLLDRFLALGTQHPVGGFFAGISK
PAPDQLDTAHPYVLGIPQPTTDRLLAEHATALGADIRRGHELVGLNQDDDTVTAHLADGTHLHARYLVGCDGGRSTVRKL
LGVPFPGEPTRHQTLLAEAELTAPPETINAIVTKVRETHKRFGAGPLGNGLYRLIAPADGVVEDRTPPTLDELKQQIRRL
AGTDFGAHSPRWLSRFGDATRQAERYRTGRVLLAGDAAHIHPPTGGQGLNLGVQDAFNLGWKLAATINGWAPAGLLDTYH
TERHPVAADVLTNTRAQMELMSLDPGAQAVRRLLVELMDFDDVNRHLTEKIIAIGIRYDFGAGHHLLGRRMRDIALKRGR
LYPLMHHGRGLLLDQTGQLSVTGWADRVDHIIDVSDELDVPAALLRPDGHIAWVGDNQQDLLGHLPTWFGTATTRAQRS

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=343, Percent_Identity=34.402332361516, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 52218; Mature: 52218

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVIVVGGGPTGLMLASELRLHGIDVRILEKDTEPTPVVRSLGLHARSIEIMDQRGLLD
CEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCEEEEEHHHCCHHH
RFLALGTQHPVGGFFAGISKPAPDQLDTAHPYVLGIPQPTTDRLLAEHATALGADIRRGH
HHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCC
ELVGLNQDDDTVTAHLADGTHLHARYLVGCDGGRSTVRKLLGVPFPGEPTRHQTLLAEAE
EEECCCCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCHHEEEECCC
LTAPPETINAIVTKVRETHKRFGAGPLGNGLYRLIAPADGVVEDRTPPTLDELKQQIRRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
AGTDFGAHSPRWLSRFGDATRQAERYRTGRVLLAGDAAHIHPPTGGQGLNLGVQDAFNLG
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCHHHHHCCC
WKLAATINGWAPAGLLDTYHTERHPVAADVLTNTRAQMELMSLDPGAQAVRRLLVELMDF
EEEEEEECCCCCCCHHHHCCCCCCCCHHHHHHCCHHHEEEEECCCCHHHHHHHHHHHHCC
DDVNRHLTEKIIAIGIRYDFGAGHHLLGRRMRDIALKRGRLYPLMHHGRGLLLDQTGQLS
CHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEHHCCCEEEEECCCCEE
VTGWADRVDHIIDVSDELDVPAALLRPDGHIAWVGDNQQDLLGHLPTWFGTATTRAQRS
EEEHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEECCCHHHHHHCCCHHHCCCCHHCCCC
>Mature Secondary Structure
MIDVIVVGGGPTGLMLASELRLHGIDVRILEKDTEPTPVVRSLGLHARSIEIMDQRGLLD
CEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCEEEEEHHHCCHHH
RFLALGTQHPVGGFFAGISKPAPDQLDTAHPYVLGIPQPTTDRLLAEHATALGADIRRGH
HHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCC
ELVGLNQDDDTVTAHLADGTHLHARYLVGCDGGRSTVRKLLGVPFPGEPTRHQTLLAEAE
EEECCCCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCHHEEEECCC
LTAPPETINAIVTKVRETHKRFGAGPLGNGLYRLIAPADGVVEDRTPPTLDELKQQIRRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
AGTDFGAHSPRWLSRFGDATRQAERYRTGRVLLAGDAAHIHPPTGGQGLNLGVQDAFNLG
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCHHHHHCCC
WKLAATINGWAPAGLLDTYHTERHPVAADVLTNTRAQMELMSLDPGAQAVRRLLVELMDF
EEEEEEECCCCCCCHHHHCCCCCCCCHHHHHHCCHHHEEEEECCCCHHHHHHHHHHHHCC
DDVNRHLTEKIIAIGIRYDFGAGHHLLGRRMRDIALKRGRLYPLMHHGRGLLLDQTGQLS
CHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEHHCCCEEEEECCCCEE
VTGWADRVDHIIDVSDELDVPAALLRPDGHIAWVGDNQQDLLGHLPTWFGTATTRAQRS
EEEHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEECCCHHHHHHCCCHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]