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The map label for this gene is pur8 [H]

Identifier: 158315775

GI number: 158315775

Start: 4772891

End: 4774339

Strand: Reverse

Name: pur8 [H]

Synonym: Franean1_3988

Alternate gene names: 158315775

Gene position: 4774339-4772891 (Counterclockwise)

Preceding gene: 158315777

Following gene: 158315771

Centisome position: 53.15

GC content: 68.18

Gene sequence:

>1449_bases
ATGCGGATAGCCGCGCACCCTTCGGCCGAGCCGGCCCCGACCACAGCTGACCCGCTGCGTTGGCGGGCACTGGCCGCGAT
AGCCGCCGGCCAGCTCATGATTGCGGCAGACGTCACGATCATGAATATCGCACTGCCGTCCGCCCAGCACTCCTTGCACC
TGTCAACGGCGCAACGACAGTGGGTGATCACCCTCTTCGCTCTCGCCTACGGCGGGTTCCTCCTGCTCGGGGGCAGACTG
TCCGATCTGATCGGTCGCAAGCGCTGCCTGCTGATCGGACTGGCGGGTTTCGCCGCAGCCTCGGCGCTGGGTGGGGCAGC
CGTGAACCCAACCATGCTCCTGGTTGCTAGAGCGCTCCAGGGCATATTCGGGGCGCTGTTCACACCCTCCGCCCTCGCGC
TGCTCGGTACGACATTCACCGAACCCTCCGAACGCGGCAAAGCCTTCGGGATCTACGGCACCGTGATGGCGGGCAGTTCC
GGCATCGGACTGATCCTCGGCGGCGTCCTCACCAACTACCTCGACTGGCGCTGGTGCATGCTCGTGAGCCTGCCCATCGC
GGTCGGCGCTGCCGCCGGAGTCAGCGCGACGGTTCGCGCGACCCCCCGCCGGCTCGGCACCGAGGTAGACATCGTCGGCG
CGGTGCTCGCCACAACCGGGCTCATGGCACTGGTCCTTGGATTCACCCGCGCGGAGTCACAAGGCTGGGCCACCCGGATC
ACGCTGGGCGTTCTTGCCGCCGGAGTCATCCTTCTCGCGCTGTTCGTCCTAGTGGAAAGCCGCACCGGAGCGGCCCTCCT
GCCGCTGCGGGTCGTCCGTGAGCGTCGACGAGCCGGTGCGTACCTGGCCGTCCTGTGCATGGCGATCGGCATGTTCGCCG
GATTCTTCTTCCTCACCTTCTACCTGCAGGACATCCTCGGATACTCACCGATCAAGGCAGGACTCGCGTTCCTCCCGTTC
ACTGCGGCGATCATGCTAGGAGTACGCGTCATCCGCGGGTTCCTGATGCGCGCACCCCTGCGGCTGCTGCTGTGCCCGGG
TCTCCTGGCATGCGCGGCCGGACTCGCACTGCTCGGCCTACTACGCGCCGACGGCGGCTACGTCACCGGGGCGCTTCCCG
TCGTCGTGCTGCTCGGACTCGGTGTCGGCTGTGTGCTGCTGCCTGCCAACAACATCGCGACTCTCGGCGCGGGCCCGGAC
ACCGGCGTCGCCGGCGCCATCGTGATGACCTCCCAACAGATCGGCGCCTCGCTCGGCACCGCCCTGCTCGGCAGCATCGC
CGCTACCGCCACCACCGCCTACGTCCACTCGCACGCCGCCGCGGCCGACCTCCCCGCACGGGCCGCGGTGCACGGCTACA
ACGTAGCCGGCCTCTCCGGCGCCGCCTTCCTGTGCCTCGCAACGACCCTGGTGTTCCTCCTTACCGGTCCGAGGAACCCC
AACCAATAA

Upstream 100 bases:

>100_bases
AGCAGCCTACCAATTTTGTTGGTCGTACCTACAAACCCTGCTAACGTTGGCCATCCCAACGTTGGACAGGCCAACGTTCA
CGAGCGCGCTGAGGTGCTGG

Downstream 100 bases:

>100_bases
AAAGATCCTCCACGGCGTTCACGGCGACTGGCTAGAACGCATCCCGCTGACCCGCAGGAACATACGCGACATCGTCAACG
CCCTCCGCGCCCACGGATAC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MRIAAHPSAEPAPTTADPLRWRALAAIAAGQLMIAADVTIMNIALPSAQHSLHLSTAQRQWVITLFALAYGGFLLLGGRL
SDLIGRKRCLLIGLAGFAAASALGGAAVNPTMLLVARALQGIFGALFTPSALALLGTTFTEPSERGKAFGIYGTVMAGSS
GIGLILGGVLTNYLDWRWCMLVSLPIAVGAAAGVSATVRATPRRLGTEVDIVGAVLATTGLMALVLGFTRAESQGWATRI
TLGVLAAGVILLALFVLVESRTGAALLPLRVVRERRRAGAYLAVLCMAIGMFAGFFFLTFYLQDILGYSPIKAGLAFLPF
TAAIMLGVRVIRGFLMRAPLRLLLCPGLLACAAGLALLGLLRADGGYVTGALPVVVLLGLGVGCVLLPANNIATLGAGPD
TGVAGAIVMTSQQIGASLGTALLGSIAATATTAYVHSHAAAADLPARAAVHGYNVAGLSGAAFLCLATTLVFLLTGPRNP
NQ

Sequences:

>Translated_482_residues
MRIAAHPSAEPAPTTADPLRWRALAAIAAGQLMIAADVTIMNIALPSAQHSLHLSTAQRQWVITLFALAYGGFLLLGGRL
SDLIGRKRCLLIGLAGFAAASALGGAAVNPTMLLVARALQGIFGALFTPSALALLGTTFTEPSERGKAFGIYGTVMAGSS
GIGLILGGVLTNYLDWRWCMLVSLPIAVGAAAGVSATVRATPRRLGTEVDIVGAVLATTGLMALVLGFTRAESQGWATRI
TLGVLAAGVILLALFVLVESRTGAALLPLRVVRERRRAGAYLAVLCMAIGMFAGFFFLTFYLQDILGYSPIKAGLAFLPF
TAAIMLGVRVIRGFLMRAPLRLLLCPGLLACAAGLALLGLLRADGGYVTGALPVVVLLGLGVGCVLLPANNIATLGAGPD
TGVAGAIVMTSQQIGASLGTALLGSIAATATTAYVHSHAAAADLPARAAVHGYNVAGLSGAAFLCLATTLVFLLTGPRNP
NQ
>Mature_482_residues
MRIAAHPSAEPAPTTADPLRWRALAAIAAGQLMIAADVTIMNIALPSAQHSLHLSTAQRQWVITLFALAYGGFLLLGGRL
SDLIGRKRCLLIGLAGFAAASALGGAAVNPTMLLVARALQGIFGALFTPSALALLGTTFTEPSERGKAFGIYGTVMAGSS
GIGLILGGVLTNYLDWRWCMLVSLPIAVGAAAGVSATVRATPRRLGTEVDIVGAVLATTGLMALVLGFTRAESQGWATRI
TLGVLAAGVILLALFVLVESRTGAALLPLRVVRERRRAGAYLAVLCMAIGMFAGFFFLTFYLQDILGYSPIKAGLAFLPF
TAAIMLGVRVIRGFLMRAPLRLLLCPGLLACAAGLALLGLLRADGGYVTGALPVVVLLGLGVGCVLLPANNIATLGAGPD
TGVAGAIVMTSQQIGASLGTALLGSIAATATTAYVHSHAAAADLPARAAVHGYNVAGLSGAAFLCLATTLVFLLTGPRNP
NQ

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=425, Percent_Identity=25.8823529411765, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=27.2289156626506, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789042, Length=422, Percent_Identity=24.1706161137441, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790195, Length=408, Percent_Identity=27.2058823529412, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788392, Length=421, Percent_Identity=28.5035629453682, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=392, Percent_Identity=25, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319770, Length=280, Percent_Identity=27.5, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323520, Length=401, Percent_Identity=24.4389027431421, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=228, Percent_Identity=25.4385964912281, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323935, Length=408, Percent_Identity=25.2450980392157, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=132, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49259; Mature: 49259

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAAHPSAEPAPTTADPLRWRALAAIAAGQLMIAADVTIMNIALPSAQHSLHLSTAQRQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCCEEEHHHHHH
WVITLFALAYGGFLLLGGRLSDLIGRKRCLLIGLAGFAAASALGGAAVNPTMLLVARALQ
HHHHHHHHHHCCCEEECCHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHH
GIFGALFTPSALALLGTTFTEPSERGKAFGIYGTVMAGSSGIGLILGGVLTNYLDWRWCM
HHHHHHHCHHHHHHHHCCCCCHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LVSLPIAVGAAAGVSATVRATPRRLGTEVDIVGAVLATTGLMALVLGFTRAESQGWATRI
HHHHHHHHHHHCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TLGVLAAGVILLALFVLVESRTGAALLPLRVVRERRRAGAYLAVLCMAIGMFAGFFFLTF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQDILGYSPIKAGLAFLPFTAAIMLGVRVIRGFLMRAPLRLLLCPGLLACAAGLALLGL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRADGGYVTGALPVVVLLGLGVGCVLLPANNIATLGAGPDTGVAGAIVMTSQQIGASLGT
HHCCCCEEHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCEEEEECHHHHHHHHH
ALLGSIAATATTAYVHSHAAAADLPARAAVHGYNVAGLSGAAFLCLATTLVFLLTGPRNP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
NQ
CC
>Mature Secondary Structure
MRIAAHPSAEPAPTTADPLRWRALAAIAAGQLMIAADVTIMNIALPSAQHSLHLSTAQRQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCCEEEHHHHHH
WVITLFALAYGGFLLLGGRLSDLIGRKRCLLIGLAGFAAASALGGAAVNPTMLLVARALQ
HHHHHHHHHHCCCEEECCHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHH
GIFGALFTPSALALLGTTFTEPSERGKAFGIYGTVMAGSSGIGLILGGVLTNYLDWRWCM
HHHHHHHCHHHHHHHHCCCCCHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LVSLPIAVGAAAGVSATVRATPRRLGTEVDIVGAVLATTGLMALVLGFTRAESQGWATRI
HHHHHHHHHHHCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TLGVLAAGVILLALFVLVESRTGAALLPLRVVRERRRAGAYLAVLCMAIGMFAGFFFLTF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQDILGYSPIKAGLAFLPFTAAIMLGVRVIRGFLMRAPLRLLLCPGLLACAAGLALLGL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRADGGYVTGALPVVVLLGLGVGCVLLPANNIATLGAGPDTGVAGAIVMTSQQIGASLGT
HHCCCCEEHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCEEEEECHHHHHHHHH
ALLGSIAATATTAYVHSHAAAADLPARAAVHGYNVAGLSGAAFLCLATTLVFLLTGPRNP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]