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The map label for this gene is lcfB [H]

Identifier: 158315501

GI number: 158315501

Start: 4387761

End: 4389329

Strand: Reverse

Name: lcfB [H]

Synonym: Franean1_3706

Alternate gene names: 158315501

Gene position: 4389329-4387761 (Counterclockwise)

Preceding gene: 158315505

Following gene: 158315500

Centisome position: 48.87

GC content: 75.72

Gene sequence:

>1569_bases
GTGAGCAGCACTCGTGAAACACCGCAGCGCACGGATCCGCAGCCCCTGAGCGGGACGTCCGCCCAGGTCGCGCGGGTCCC
CGGCCCGATCGCCGGCGTGCTCGACGCCGCTCTCGCGGCCCGCCCGCAGGCCGCGGCGGTCGAGGCGGTCTCGGGTATCT
GGTCGTACGCCGAGCTCGACGAGCAGGCGGACCGCGCGGCCGGCGCGTTGTGGTCGCTCGGCGTGCGCCCGGGTGACCGC
GTCGCGGCCTGCCTGCCCAACGACCTCGACATCGTCGCCGCGTTCCACGGCGCGCAGCGGATCGGCGCCGTCTGGGCGGG
GATCGGCGAGGCGCTCGCCGCGAGCGAGCAGCAGGAGCTGCACGAGATCTGCGATCCCACCGTCGTGCTGGCCGGCCCCC
GCTACCTGCTGGACTCCCCCGGCCGCGTCGACCCCGACCGCTGGGCGGGCCTGCTCGCCCGCGGCGAGCGCGCCCCCCGG
GTCGAGCACGACATCGACGCCCCGGCCGGCATCGCGTTCACCAGCGGGACGTCGGGACTTCCCAAGGCGGTCGTGCACAG
CCAGCGCAACCTGCTCCTGCCGGGCGCGGTGCTCGTGGCGACCCGGGGCTGGGGCCCCGAGCTGCGCAAGGGCGACAGCT
TCCCGTTCACGATCCTCAACCTGATGGTGCTCTCCACCCTGCTCACGGCGCAGGCCGGCGGGTGCGCGGTGGTGATGAAC
CGCCGGGACGTCGCCGGCGTCGTCGAGTGGATCTCCAGCCGGCAGGTCAACGTGTGGAACGGCGCGCCCGCGCAGCTGCA
CGACCTCGCCGCCCGACCCGAGGCCGGGATCGGCTCGCTGCGGGAGGTCTGGAGCGGCGGCAGCGACACCGCGGACGCGC
TGCGCCGGGCGTTCGCCGACGCGCACGGGCTGGCGCCCCGGGCCACCTACGGCCTGACCGAGGCACCGACCGTCGTGTCG
ATCGACCCGCCGGGCGACGAGTGGCGCCCGGGGACGAGCGGCCAGGTGCTGCCGCACTACGACGTGGCGGCCTACGACGA
CGAGGGCCGCCGGCTGCCCGCCGGTGAGCTCGGCGAGCTGCGGCTGAGCGGCGCCACGACCGGCCCGTGGGCCGGCGTCT
GGCGGCCGACGCTCGGGTTCTGGGAGAACGGCGCCGTCCGGCCGCTGGAGCCGGGCCCGATCGCCACCGGCGACATCGGG
ACCGTCGACGCCGACGGGTGGCTGACCGTGCTCGACCGCAAGAAGCTGATCATCATCCGGGGCGGCGCGAACGTCTACCC
GCTCGAGGTGGAGCGGGTCATCGCGACGTACCCGGATGTCGCCCGGGTCGCGGTGTGCGGGGTGCCCGACGAGCGGCTCG
GGCAGCGGGTGGCCGCGGTGATCGAGAGCGGCACCGGCGGCCCGCTCGACACCGGCGCGCTCACCGAGCTGTGCCGGCGC
GAGCTCTCGCCGTACAAGGTGCCGGAGTTCTGGACCCAGGTCGACGCCCTGCCGGTGAACGCGATGGGCAAGGTACAGCG
CACCGGGCTGGCCGAGCTCGTCGGCCAGCCGCGCCCCGCCGGAAGCTAG

Upstream 100 bases:

>100_bases
TATCGACCACTCCTCGATCGACAGGACCCGGCCGGCCAGGAACCGCGCCACGCCGAGAATAAGGGATGAATCCTTGTAAG
GATAGTGATATCAAGATGTC

Downstream 100 bases:

>100_bases
GGCCGGGGGACGAAGAGTGAGCATCCGGCTGAGCGAGGACGAGATCTGGGAGTTCGTCGGCGCCGCGCACACGGGGGTGC
TCACCACCCTGCGCGCGGAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSSTRETPQRTDPQPLSGTSAQVARVPGPIAGVLDAALAARPQAAAVEAVSGIWSYAELDEQADRAAGALWSLGVRPGDR
VAACLPNDLDIVAAFHGAQRIGAVWAGIGEALAASEQQELHEICDPTVVLAGPRYLLDSPGRVDPDRWAGLLARGERAPR
VEHDIDAPAGIAFTSGTSGLPKAVVHSQRNLLLPGAVLVATRGWGPELRKGDSFPFTILNLMVLSTLLTAQAGGCAVVMN
RRDVAGVVEWISSRQVNVWNGAPAQLHDLAARPEAGIGSLREVWSGGSDTADALRRAFADAHGLAPRATYGLTEAPTVVS
IDPPGDEWRPGTSGQVLPHYDVAAYDDEGRRLPAGELGELRLSGATTGPWAGVWRPTLGFWENGAVRPLEPGPIATGDIG
TVDADGWLTVLDRKKLIIIRGGANVYPLEVERVIATYPDVARVAVCGVPDERLGQRVAAVIESGTGGPLDTGALTELCRR
ELSPYKVPEFWTQVDALPVNAMGKVQRTGLAELVGQPRPAGS

Sequences:

>Translated_522_residues
MSSTRETPQRTDPQPLSGTSAQVARVPGPIAGVLDAALAARPQAAAVEAVSGIWSYAELDEQADRAAGALWSLGVRPGDR
VAACLPNDLDIVAAFHGAQRIGAVWAGIGEALAASEQQELHEICDPTVVLAGPRYLLDSPGRVDPDRWAGLLARGERAPR
VEHDIDAPAGIAFTSGTSGLPKAVVHSQRNLLLPGAVLVATRGWGPELRKGDSFPFTILNLMVLSTLLTAQAGGCAVVMN
RRDVAGVVEWISSRQVNVWNGAPAQLHDLAARPEAGIGSLREVWSGGSDTADALRRAFADAHGLAPRATYGLTEAPTVVS
IDPPGDEWRPGTSGQVLPHYDVAAYDDEGRRLPAGELGELRLSGATTGPWAGVWRPTLGFWENGAVRPLEPGPIATGDIG
TVDADGWLTVLDRKKLIIIRGGANVYPLEVERVIATYPDVARVAVCGVPDERLGQRVAAVIESGTGGPLDTGALTELCRR
ELSPYKVPEFWTQVDALPVNAMGKVQRTGLAELVGQPRPAGS
>Mature_521_residues
SSTRETPQRTDPQPLSGTSAQVARVPGPIAGVLDAALAARPQAAAVEAVSGIWSYAELDEQADRAAGALWSLGVRPGDRV
AACLPNDLDIVAAFHGAQRIGAVWAGIGEALAASEQQELHEICDPTVVLAGPRYLLDSPGRVDPDRWAGLLARGERAPRV
EHDIDAPAGIAFTSGTSGLPKAVVHSQRNLLLPGAVLVATRGWGPELRKGDSFPFTILNLMVLSTLLTAQAGGCAVVMNR
RDVAGVVEWISSRQVNVWNGAPAQLHDLAARPEAGIGSLREVWSGGSDTADALRRAFADAHGLAPRATYGLTEAPTVVSI
DPPGDEWRPGTSGQVLPHYDVAAYDDEGRRLPAGELGELRLSGATTGPWAGVWRPTLGFWENGAVRPLEPGPIATGDIGT
VDADGWLTVLDRKKLIIIRGGANVYPLEVERVIATYPDVARVAVCGVPDERLGQRVAAVIESGTGGPLDTGALTELCRRE
LSPYKVPEFWTQVDALPVNAMGKVQRTGLAELVGQPRPAGS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=526, Percent_Identity=25.4752851711027, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI38505220, Length=492, Percent_Identity=27.4390243902439, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI122937307, Length=503, Percent_Identity=26.2425447316103, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157311624, Length=494, Percent_Identity=26.3157894736842, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=494, Percent_Identity=26.3157894736842, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI58082049, Length=494, Percent_Identity=25.5060728744939, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI187761345, Length=540, Percent_Identity=26.8518518518519, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI187761343, Length=540, Percent_Identity=26.8518518518519, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI115511026, Length=363, Percent_Identity=26.1707988980716, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI42544132, Length=372, Percent_Identity=24.1935483870968, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1788107, Length=538, Percent_Identity=25.6505576208178, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI221142682, Length=497, Percent_Identity=26.9617706237425, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI145693145, Length=519, Percent_Identity=24.2774566473988, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786810, Length=496, Percent_Identity=22.7822580645161, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=470, Percent_Identity=25.9574468085106, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=525, Percent_Identity=23.6190476190476, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=525, Percent_Identity=23.6190476190476, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=525, Percent_Identity=23.4285714285714, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=485, Percent_Identity=25.7731958762887, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=524, Percent_Identity=23.6641221374046, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=479, Percent_Identity=25.2609603340292, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=511, Percent_Identity=25.0489236790607, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=507, Percent_Identity=25.2465483234714, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=394, Percent_Identity=23.8578680203046, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=501, Percent_Identity=22.9540918163673, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=356, Percent_Identity=26.4044943820225, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581924, Length=519, Percent_Identity=25.8188824662813, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI20130357, Length=368, Percent_Identity=27.7173913043478, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648257, Length=352, Percent_Identity=28.9772727272727, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21356441, Length=462, Percent_Identity=24.6753246753247, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21355181, Length=552, Percent_Identity=23.3695652173913, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653035, Length=371, Percent_Identity=25.8760107816712, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648255, Length=522, Percent_Identity=22.9885057471264, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648253, Length=522, Percent_Identity=22.9885057471264, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281365686, Length=339, Percent_Identity=25.3687315634218, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21356947, Length=352, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21358303, Length=351, Percent_Identity=23.9316239316239, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=367, Percent_Identity=24.7956403269755, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648260, Length=324, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54920; Mature: 54788

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTRETPQRTDPQPLSGTSAQVARVPGPIAGVLDAALAARPQAAAVEAVSGIWSYAELD
CCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EQADRAAGALWSLGVRPGDRVAACLPNDLDIVAAFHGAQRIGAVWAGIGEALAASEQQEL
HHHHHHHHHHHCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEICDPTVVLAGPRYLLDSPGRVDPDRWAGLLARGERAPRVEHDIDAPAGIAFTSGTSGL
HHHCCCEEEEECCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCC
PKAVVHSQRNLLLPGAVLVATRGWGPELRKGDSFPFTILNLMVLSTLLTAQAGGCAVVMN
CHHHHHCCCCEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
RRDVAGVVEWISSRQVNVWNGAPAQLHDLAARPEAGIGSLREVWSGGSDTADALRRAFAD
CCHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
AHGLAPRATYGLTEAPTVVSIDPPGDEWRPGTSGQVLPHYDVAAYDDEGRRLPAGELGEL
HHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCE
RLSGATTGPWAGVWRPTLGFWENGAVRPLEPGPIATGDIGTVDADGWLTVLDRKKLIIIR
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCCEEEEEE
GGANVYPLEVERVIATYPDVARVAVCGVPDERLGQRVAAVIESGTGGPLDTGALTELCRR
CCCCEEEHHHHHHHHCCCCHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
ELSPYKVPEFWTQVDALPVNAMGKVQRTGLAELVGQPRPAGS
HCCCCCCHHHHHHHCCCCCCHHHHHHHCCHHHHHCCCCCCCC
>Mature Secondary Structure 
SSTRETPQRTDPQPLSGTSAQVARVPGPIAGVLDAALAARPQAAAVEAVSGIWSYAELD
CCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EQADRAAGALWSLGVRPGDRVAACLPNDLDIVAAFHGAQRIGAVWAGIGEALAASEQQEL
HHHHHHHHHHHCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEICDPTVVLAGPRYLLDSPGRVDPDRWAGLLARGERAPRVEHDIDAPAGIAFTSGTSGL
HHHCCCEEEEECCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCC
PKAVVHSQRNLLLPGAVLVATRGWGPELRKGDSFPFTILNLMVLSTLLTAQAGGCAVVMN
CHHHHHCCCCEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
RRDVAGVVEWISSRQVNVWNGAPAQLHDLAARPEAGIGSLREVWSGGSDTADALRRAFAD
CCHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
AHGLAPRATYGLTEAPTVVSIDPPGDEWRPGTSGQVLPHYDVAAYDDEGRRLPAGELGEL
HHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCE
RLSGATTGPWAGVWRPTLGFWENGAVRPLEPGPIATGDIGTVDADGWLTVLDRKKLIIIR
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCCEEEEEE
GGANVYPLEVERVIATYPDVARVAVCGVPDERLGQRVAAVIESGTGGPLDTGALTELCRR
CCCCEEEHHHHHHHHCCCCHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
ELSPYKVPEFWTQVDALPVNAMGKVQRTGLAELVGQPRPAGS
HCCCCCCHHHHHHHCCCCCCHHHHHHHCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]