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The map label for this gene is ynfL [H]

Identifier: 158315328

GI number: 158315328

Start: 4189363

End: 4190262

Strand: Reverse

Name: ynfL [H]

Synonym: Franean1_3529

Alternate gene names: 158315328

Gene position: 4190262-4189363 (Counterclockwise)

Preceding gene: 158315334

Following gene: 158315327

Centisome position: 46.65

GC content: 70.67

Gene sequence:

>900_bases
ATGGATCTGGACCTGCGCAAACTGCGCTACTTCCTCGCCGTCGCCGACAGGCTGCACTTCGGCCGCGCCGCTGAAGAGCT
GCATATCGCGCAGCCGGTGCTCAGTCGGCAGATCCGCGCGCTGGAACAGGATCTCGGCGCCTCACTGTTCACCAGGGATC
GCCACGGCGTGGTGTTGACCGACGCCGGCCGACAACTGCTGGCCGACGCCGGTCCGCTGCTCGCCTCCGCGGACGCGGTC
CGCCGCCGGGTGACCGGGGCCGCCCGCGGCAGCCGGCGGCTGGCGGTCGGCTTCCGGACCGGCATCCCGGTCATCCCAGC
GGCCCGGGCGTTCGAGGCCCGACATCCGGACGTGGTCGTGGACGTGCAGCGGATCGAATGGGACGACCAGGCCTCGATGC
TGCTCGACGGCCGCATCGACGTCGGCTATGTGCGGCTGCCCATCGACGAGACCGGCCTGCGCGTGACCCCGCTGTACACC
GAGCCGCTCATGGTGGTGCTGCCCGCAGGCCACCGGTTGGCCGGCAAGGAGGAGGTCACCGAGGCCGACCTGGCCGGTGA
GCCGCTGGTCTGGCATGCCGACCCGAGCACGCAGCCCACCAGGCGCCCGCACCCCGACTCCGGGCTCCGGGTACGCGGCG
TGGAGGAGAAGCTCGAGCACGTCGCGGCCGGCCGGGGCATCTCGTTCGTGGGGCGTTCGGAGACAGTGTTCTACTCGCGT
CCGGACATCAGCTACGTACCCATCCCGGACCTCGCGCCCGACCAGGTGTGCCTCGCGATGGCGGCATCGCGCACCTCGCC
GCTGGCCGACGACTTCTTCGCCGCGGCGCAGGCGACGGCCGAGATCACGGCAGAATGTGGGAACTATGATTTGGGTGCGG
ATCTTCTGAAGGGACGCTGA

Upstream 100 bases:

>100_bases
CATTAGGTACCTATTAAGGTTGCCATTGACCTGAGTGGGTGTCCAAGACTTCTTTCAGCTGTGGTGATACCCTGGAGGTA
TCACCGGACCGGGAGGTTCC

Downstream 100 bases:

>100_bases
GATGGCCGGTCACCTGCGAGTTCCGCAACCTGGGTGATCTTGATGGGCTCCTTCCCGCTACGTTCGTGGGTGCTTGAAGT
CCATGAACCACAAGAAGGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDLDLRKLRYFLAVADRLHFGRAAEELHIAQPVLSRQIRALEQDLGASLFTRDRHGVVLTDAGRQLLADAGPLLASADAV
RRRVTGAARGSRRLAVGFRTGIPVIPAARAFEARHPDVVVDVQRIEWDDQASMLLDGRIDVGYVRLPIDETGLRVTPLYT
EPLMVVLPAGHRLAGKEEVTEADLAGEPLVWHADPSTQPTRRPHPDSGLRVRGVEEKLEHVAAGRGISFVGRSETVFYSR
PDISYVPIPDLAPDQVCLAMAASRTSPLADDFFAAAQATAEITAECGNYDLGADLLKGR

Sequences:

>Translated_299_residues
MDLDLRKLRYFLAVADRLHFGRAAEELHIAQPVLSRQIRALEQDLGASLFTRDRHGVVLTDAGRQLLADAGPLLASADAV
RRRVTGAARGSRRLAVGFRTGIPVIPAARAFEARHPDVVVDVQRIEWDDQASMLLDGRIDVGYVRLPIDETGLRVTPLYT
EPLMVVLPAGHRLAGKEEVTEADLAGEPLVWHADPSTQPTRRPHPDSGLRVRGVEEKLEHVAAGRGISFVGRSETVFYSR
PDISYVPIPDLAPDQVCLAMAASRTSPLADDFFAAAQATAEITAECGNYDLGADLLKGR
>Mature_299_residues
MDLDLRKLRYFLAVADRLHFGRAAEELHIAQPVLSRQIRALEQDLGASLFTRDRHGVVLTDAGRQLLADAGPLLASADAV
RRRVTGAARGSRRLAVGFRTGIPVIPAARAFEARHPDVVVDVQRIEWDDQASMLLDGRIDVGYVRLPIDETGLRVTPLYT
EPLMVVLPAGHRLAGKEEVTEADLAGEPLVWHADPSTQPTRRPHPDSGLRVRGVEEKLEHVAAGRGISFVGRSETVFYSR
PDISYVPIPDLAPDQVCLAMAASRTSPLADDFFAAAQATAEITAECGNYDLGADLLKGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=196, Percent_Identity=40.3061224489796, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1788887, Length=304, Percent_Identity=31.9078947368421, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI157672245, Length=189, Percent_Identity=38.0952380952381, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI2367136, Length=286, Percent_Identity=30.4195804195804, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=26.9387755102041, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI145693105, Length=240, Percent_Identity=28.3333333333333, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081904, Length=217, Percent_Identity=31.3364055299539, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994962, Length=87, Percent_Identity=43.6781609195402, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788297, Length=169, Percent_Identity=28.4023668639053, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787806, Length=171, Percent_Identity=30.4093567251462, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790208, Length=64, Percent_Identity=51.5625, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789173, Length=188, Percent_Identity=31.9148936170213, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=199, Percent_Identity=28.643216080402, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790262, Length=190, Percent_Identity=27.8947368421053, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=28.3400809716599, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787589, Length=146, Percent_Identity=34.9315068493151, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32590; Mature: 32590

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDLRKLRYFLAVADRLHFGRAAEELHIAQPVLSRQIRALEQDLGASLFTRDRHGVVLT
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCEEEE
DAGRQLLADAGPLLASADAVRRRVTGAARGSRRLAVGFRTGIPVIPAARAFEARHPDVVV
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCCEEE
DVQRIEWDDQASMLLDGRIDVGYVRLPIDETGLRVTPLYTEPLMVVLPAGHRLAGKEEVT
EEEEECCCCCCCEEECCCCCEEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCCHHHH
EADLAGEPLVWHADPSTQPTRRPHPDSGLRVRGVEEKLEHVAAGRGISFVGRSETVFYSR
HHHCCCCCEEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCEEEECCCCEEEEEC
PDISYVPIPDLAPDQVCLAMAASRTSPLADDFFAAAQATAEITAECGNYDLGADLLKGR
CCCCEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MDLDLRKLRYFLAVADRLHFGRAAEELHIAQPVLSRQIRALEQDLGASLFTRDRHGVVLT
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCEEEE
DAGRQLLADAGPLLASADAVRRRVTGAARGSRRLAVGFRTGIPVIPAARAFEARHPDVVV
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCCEEE
DVQRIEWDDQASMLLDGRIDVGYVRLPIDETGLRVTPLYTEPLMVVLPAGHRLAGKEEVT
EEEEECCCCCCCEEECCCCCEEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCCHHHH
EADLAGEPLVWHADPSTQPTRRPHPDSGLRVRGVEEKLEHVAAGRGISFVGRSETVFYSR
HHHCCCCCEEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCEEEECCCCEEEEEC
PDISYVPIPDLAPDQVCLAMAASRTSPLADDFFAAAQATAEITAECGNYDLGADLLKGR
CCCCEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]