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The map label for this gene is emrB [H]

Identifier: 158315281

GI number: 158315281

Start: 4139422

End: 4140879

Strand: Reverse

Name: emrB [H]

Synonym: Franean1_3481

Alternate gene names: 158315281

Gene position: 4140879-4139422 (Counterclockwise)

Preceding gene: 158315283

Following gene: 158315280

Centisome position: 46.1

GC content: 73.73

Gene sequence:

>1458_bases
ATGGTAGGCACGGACACGGCGGAGGACAGCCCCCCTCGGCTCGATTCCGCCCTGCTGATCCTCATCGGGGTGATGGTGCT
CGGCGGGATGATGTCCTACCTGGACGCGACCATCGTCAACGTCGGGATCAGCACGCTCGGCGCGGAGTTCGAGGCCTCGC
TCGCCACGATCGAGTGGGTGACCACCGGCTACCTGCTGGCGGTCGCGCTCGCCATCCCGCTGGCCGGCTGGGCGATGGCG
CGCTTCGGCTCGCGGCGCATGTGGCTGCTCGGCCTGTCGCTGTTCCTCGCCGGCTCGGCGCTGTGCGCGGTGGCCTGGAA
CGCGGAGAGCCTGATCGCCTTCCGGGTCCTGCAGGGGCTCGGCGGCGGCATGGTCGACCCGATCATGATGACCGTGGTGG
CCGGCGCCGCGGGGCCGGCCCGCATGGGGCGGGTGATGGGCCTGATCTCGGTCCCGATCACGCTCGGGCCGGTGCTCGGC
CCGATCGTCGGCGGTGTGCTGCTGGAGAACTTCTCCTGGGAGTGGATGTTCCTGGTGAACATCCCGTTCGCCCTGGCCGC
GATCGTGCTCGCCATCGTGGTGCTGCCCGCCGACCCGCCGCGGGACGGCACACCGGTGCCCCTCGACGCCCTCGGCGTGG
CCCTGCTGTGCCCCGGCTTCGCCGCGGTCGTCTTCGCGCTCTCCCAGGCCGGGCGGGACGGCTTCGGCAGCGTCCAGGTC
GTGATCGGCCTGGTCCTCGGTGCCGCGCTGTTCGCCGGGTACGCCGCGCACGCCCTGCGCACCGCCGGCACCCCGCTGCT
CGACCTGCGGCTGTTCGCCAGCAAGGGCTTCAGCGCCAGCGTGACCATCATGTTCCTGGTCGGCGGCGGCCTGTTCGCGC
TGCTGTTCCTGCTGCCGCTCTACTACCAGCAGGTGCACGACCACTCGGTGCTGGAGTCCGGCCTGCTGCTGGCCCCGCTG
GGCGTGGGCACCATGATCGGCATGCCGCTGGCCGGCAACCTCGCCGACCGCATCGGCGCGCGGCTGCTGGTGCCCACCGG
CGCCCTGCTGGTCACCGCGGGCGCCCTGGTGTTCACCCAGTCCGGGGCGGACACCTCCCAGGCCCTGCTGACCCCCGCCC
AGCTCGTCATCGGCTTCGGGCTCGGGCTGGTCGGCGCGCCCACGATGGGCTCGGTGTACCGCACCGTCCCGCTGACGGCG
GTCGCCGGCGCGACCGGCGCGGTGTTCATCCTCAACCAGATCGGCGCGTCGCTGGGCGTGGCCGTCGTGGCCCTGATCCT
GCAGGGCGGCGGGGCGCACGCCGCCCCGACGGCCGACTCGTTCGGGAACGCCTTCTGGTGGCCGGTCGCCGCCGGCCTCG
TCGTGTTCACGGCCGGCTTCCTGCTGCCCGGCCGCCCGGCGCCGGCCCCCGAGGCGGCCCCGGCCGCCCCCGCGGAGCCG
GTGCCCAACTCGGTCTGA

Upstream 100 bases:

>100_bases
GGTGGTCTCGCCCACACCCATGCACGCATGCTACCGTCCGACCCATCACATTGAAACGAGCAGTTCACTTTTACTCAAGA
TCGGTTTCAAGGAGGGTTCG

Downstream 100 bases:

>100_bases
GCCCGCGCCGGACCGCTCTCCCCGGACCGGTCCCGGAGCCCCTGACCCGGCGCCCGGGACCACCCCTCGCCCGCCGCACG
CACCCAAGCACGCACGGCTC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MVGTDTAEDSPPRLDSALLILIGVMVLGGMMSYLDATIVNVGISTLGAEFEASLATIEWVTTGYLLAVALAIPLAGWAMA
RFGSRRMWLLGLSLFLAGSALCAVAWNAESLIAFRVLQGLGGGMVDPIMMTVVAGAAGPARMGRVMGLISVPITLGPVLG
PIVGGVLLENFSWEWMFLVNIPFALAAIVLAIVVLPADPPRDGTPVPLDALGVALLCPGFAAVVFALSQAGRDGFGSVQV
VIGLVLGAALFAGYAAHALRTAGTPLLDLRLFASKGFSASVTIMFLVGGGLFALLFLLPLYYQQVHDHSVLESGLLLAPL
GVGTMIGMPLAGNLADRIGARLLVPTGALLVTAGALVFTQSGADTSQALLTPAQLVIGFGLGLVGAPTMGSVYRTVPLTA
VAGATGAVFILNQIGASLGVAVVALILQGGGAHAAPTADSFGNAFWWPVAAGLVVFTAGFLLPGRPAPAPEAAPAAPAEP
VPNSV

Sequences:

>Translated_485_residues
MVGTDTAEDSPPRLDSALLILIGVMVLGGMMSYLDATIVNVGISTLGAEFEASLATIEWVTTGYLLAVALAIPLAGWAMA
RFGSRRMWLLGLSLFLAGSALCAVAWNAESLIAFRVLQGLGGGMVDPIMMTVVAGAAGPARMGRVMGLISVPITLGPVLG
PIVGGVLLENFSWEWMFLVNIPFALAAIVLAIVVLPADPPRDGTPVPLDALGVALLCPGFAAVVFALSQAGRDGFGSVQV
VIGLVLGAALFAGYAAHALRTAGTPLLDLRLFASKGFSASVTIMFLVGGGLFALLFLLPLYYQQVHDHSVLESGLLLAPL
GVGTMIGMPLAGNLADRIGARLLVPTGALLVTAGALVFTQSGADTSQALLTPAQLVIGFGLGLVGAPTMGSVYRTVPLTA
VAGATGAVFILNQIGASLGVAVVALILQGGGAHAAPTADSFGNAFWWPVAAGLVVFTAGFLLPGRPAPAPEAAPAAPAEP
VPNSV
>Mature_485_residues
MVGTDTAEDSPPRLDSALLILIGVMVLGGMMSYLDATIVNVGISTLGAEFEASLATIEWVTTGYLLAVALAIPLAGWAMA
RFGSRRMWLLGLSLFLAGSALCAVAWNAESLIAFRVLQGLGGGMVDPIMMTVVAGAAGPARMGRVMGLISVPITLGPVLG
PIVGGVLLENFSWEWMFLVNIPFALAAIVLAIVVLPADPPRDGTPVPLDALGVALLCPGFAAVVFALSQAGRDGFGSVQV
VIGLVLGAALFAGYAAHALRTAGTPLLDLRLFASKGFSASVTIMFLVGGGLFALLFLLPLYYQQVHDHSVLESGLLLAPL
GVGTMIGMPLAGNLADRIGARLLVPTGALLVTAGALVFTQSGADTSQALLTPAQLVIGFGLGLVGAPTMGSVYRTVPLTA
VAGATGAVFILNQIGASLGVAVVALILQGGGAHAAPTADSFGNAFWWPVAAGLVVFTAGFLLPGRPAPAPEAAPAAPAEP
VPNSV

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=338, Percent_Identity=29.585798816568, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788710, Length=329, Percent_Identity=31.6109422492401, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1790195, Length=424, Percent_Identity=29.4811320754717, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=28.169014084507, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1788392, Length=320, Percent_Identity=29.6875, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790146, Length=178, Percent_Identity=25.8426966292135, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=420, Percent_Identity=22.8571428571429, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=169, Percent_Identity=27.2189349112426, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=390, Percent_Identity=23.5897435897436, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=148, Percent_Identity=29.0540540540541, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49088; Mature: 49088

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGTDTAEDSPPRLDSALLILIGVMVLGGMMSYLDATIVNVGISTLGAEFEASLATIEWV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TTGYLLAVALAIPLAGWAMARFGSRRMWLLGLSLFLAGSALCAVAWNAESLIAFRVLQGL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHEEEEECHHHHHHHHHHHHC
GGGMVDPIMMTVVAGAAGPARMGRVMGLISVPITLGPVLGPIVGGVLLENFSWEWMFLVN
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IPFALAAIVLAIVVLPADPPRDGTPVPLDALGVALLCPGFAAVVFALSQAGRDGFGSVQV
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHH
VIGLVLGAALFAGYAAHALRTAGTPLLDLRLFASKGFSASVTIMFLVGGGLFALLFLLPL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHH
YYQQVHDHSVLESGLLLAPLGVGTMIGMPLAGNLADRIGARLLVPTGALLVTAGALVFTQ
HHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCCHHHHCCCEEEECCCHHHHHCCHHEEEE
SGADTSQALLTPAQLVIGFGLGLVGAPTMGSVYRTVPLTAVAGATGAVFILNQIGASLGV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHH
AVVALILQGGGAHAAPTADSFGNAFWWPVAAGLVVFTAGFLLPGRPAPAPEAAPAAPAEP
HHHHHHHCCCCCCCCCCCHHCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VPNSV
CCCCC
>Mature Secondary Structure
MVGTDTAEDSPPRLDSALLILIGVMVLGGMMSYLDATIVNVGISTLGAEFEASLATIEWV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TTGYLLAVALAIPLAGWAMARFGSRRMWLLGLSLFLAGSALCAVAWNAESLIAFRVLQGL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHEEEEECHHHHHHHHHHHHC
GGGMVDPIMMTVVAGAAGPARMGRVMGLISVPITLGPVLGPIVGGVLLENFSWEWMFLVN
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IPFALAAIVLAIVVLPADPPRDGTPVPLDALGVALLCPGFAAVVFALSQAGRDGFGSVQV
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHH
VIGLVLGAALFAGYAAHALRTAGTPLLDLRLFASKGFSASVTIMFLVGGGLFALLFLLPL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHH
YYQQVHDHSVLESGLLLAPLGVGTMIGMPLAGNLADRIGARLLVPTGALLVTAGALVFTQ
HHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCCHHHHCCCEEEECCCHHHHHCCHHEEEE
SGADTSQALLTPAQLVIGFGLGLVGAPTMGSVYRTVPLTAVAGATGAVFILNQIGASLGV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHH
AVVALILQGGGAHAAPTADSFGNAFWWPVAAGLVVFTAGFLLPGRPAPAPEAAPAAPAEP
HHHHHHHCCCCCCCCCCCHHCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VPNSV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]