Click here to switch to the map view.

The map label for this gene is nikA [H]

Identifier: 158315279

GI number: 158315279

Start: 4136531

End: 4138171

Strand: Reverse

Name: nikA [H]

Synonym: Franean1_3479

Alternate gene names: 158315279

Gene position: 4138171-4136531 (Counterclockwise)

Preceding gene: 158315280

Following gene: 158315278

Centisome position: 46.07

GC content: 69.47

Gene sequence:

>1641_bases
ATGCGATCGCGCCGAACGGCACGAAGAGCAGGGCTGTTCGCGGCCCTCGCCGTCACCCTGGCCACCGCGGCCTGCGGATC
CGACGGCGGCGGGAGCGGCACCGCGGACGCCCAGCCACGCGCTGGCGGAAGCGTCACCTACGCCGCCCGGCAGGAGCCGG
ACTGCTGGGACCCCCATGCCAGTGCCCAGGACGTCACCGCGTTCGCACAGCGCTCGGTGTTCGACTCGCTCGTCTACCAG
ACGCCCGACGGCGCGTTCGAGCCGTGGCTGGCGAAGTCCTGGAAGATCAGCGACGACGGCCGCACCTACACCTTCGAGCT
GCGCGACGACGTCACCTTCCACGACGGCGCCAAGCTCGACGCGGAGGCGGTCAAGGCGAACTTCGACCACATCATGGCCA
AGGACACCGAGTCGCAGTTCGCCGCCGGGCTGCTCGGGCCGTACGAGGGCGTGAAGGTCACCGGCCCGCAGGAGATCCAG
GTCTCGTTCAGCCGTCCCTATGCGCCGTTGCTGCAGGTCGTCAGCACCACCTTCCTCGGCATCGCCTCACCGGCGTCGCT
GAAGGCCGGCTCCGAGAAGCTGTGCTCGGGCACCGACTCGATCGGGTCGGGGCCGTTCAAGGCCGACGCCTACACCCGCG
GCCAGCAGCGCTCCTACACCCGGTACGCCGACTACGACTGGGCACCGAAGAGCGCCGGGCACAGCGGCCCCGCCCGGCTG
GACTCGGTCACGATCCGGTTCATCACCGAGGAGGCCACCCGGGTCGGCGCGCTCAGCTCCGGCCAGGTGGACGGCGCCGC
CGACATCCCGGCCAACCAGATCGCCTCGGTCAGCAAGAACCCGCGGCTGACCACGATCAGCAAGCAGGTGCCGGGCGCCG
TCGACGCCTTCTACCTCAACACCAAGAGTGAGCTGTTCTCCGACGTCCGGGTGCGCAAGGCGTTCCAGCGCAGCCTCGAC
CTGGGCACCATCGTGAAGTCGGTGTTCCAGGGCACCACCGAGCGGGCGTGGAGCCCGCTGTCCCCGACCACGCCGAACAG
CTACGACCCGTCGCTGGAGAAGACCTGGCCGTACGACCCGAAGCTGGCCGGGCAGCTGCTCGACGAGGCCGGCTGGACTG
GGCGCGACGCCGAGGGCTACCGCACCAAGGACGGCAGGCGGCTGACCGTCTTCGCGCCGATCTACGGCGAGGCGACCGTC
TTCTCCCAGGCGGCCCAGGCCGAGCTGAAGAAGATCGGCTTCTTCCTCGACCTGCACGCCTCGACGGACGCGGCCGAGAT
CTCCGGCCTGCTGGACGGGGGGAAGTACGACACCGTCGAGCTGCAGTGGGCCCGCCCGGACGGTGACATCCTGAGCTCGT
TCTTCCTGTCCACAGAGACCTCCGTGGGCGGCGGCCACAACTTCGCCCTCGTCGCCGACCCGCAGGTCGACGAGTGGCTG
AAGGCGGCCCAGGCCGAGCAGGACCCGAAGGAGCGGGCGAAGTACTACTCCCAGGTTCAGAAGTGGACAATCGACCAGGC
CGTGGTCGTCCCGGCGTACATCAAGAACGCGACCGTCGGGGTCAACAAGAAGGTGCATGGCCTGCGGCTGAGCATCGCCA
CCTGGCCCGAGTTCTACCCCGCCTGGGTGCAGGCCGACTGA

Upstream 100 bases:

>100_bases
GCGACGGTAAGTCCAGCCACCGAGCCAGGGATTTCAGCGCGGCTTTAGAACCCCCCACCAAGGTGGTGGTCCCTATCCAT
ACCGTGTACGGAGTACTTGA

Downstream 100 bases:

>100_bases
TGCACCCGGCCATCCGTCTGGTGGCGGTCCGCCTGCTCGGCGCCGTCCTGGTCATCTGGGGCGCGGTCACCGCCGCGTTC
GTGGTGCTCCAGCTCATACC

Product: extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MRSRRTARRAGLFAALAVTLATAACGSDGGGSGTADAQPRAGGSVTYAARQEPDCWDPHASAQDVTAFAQRSVFDSLVYQ
TPDGAFEPWLAKSWKISDDGRTYTFELRDDVTFHDGAKLDAEAVKANFDHIMAKDTESQFAAGLLGPYEGVKVTGPQEIQ
VSFSRPYAPLLQVVSTTFLGIASPASLKAGSEKLCSGTDSIGSGPFKADAYTRGQQRSYTRYADYDWAPKSAGHSGPARL
DSVTIRFITEEATRVGALSSGQVDGAADIPANQIASVSKNPRLTTISKQVPGAVDAFYLNTKSELFSDVRVRKAFQRSLD
LGTIVKSVFQGTTERAWSPLSPTTPNSYDPSLEKTWPYDPKLAGQLLDEAGWTGRDAEGYRTKDGRRLTVFAPIYGEATV
FSQAAQAELKKIGFFLDLHASTDAAEISGLLDGGKYDTVELQWARPDGDILSSFFLSTETSVGGGHNFALVADPQVDEWL
KAAQAEQDPKERAKYYSQVQKWTIDQAVVVPAYIKNATVGVNKKVHGLRLSIATWPEFYPAWVQAD

Sequences:

>Translated_546_residues
MRSRRTARRAGLFAALAVTLATAACGSDGGGSGTADAQPRAGGSVTYAARQEPDCWDPHASAQDVTAFAQRSVFDSLVYQ
TPDGAFEPWLAKSWKISDDGRTYTFELRDDVTFHDGAKLDAEAVKANFDHIMAKDTESQFAAGLLGPYEGVKVTGPQEIQ
VSFSRPYAPLLQVVSTTFLGIASPASLKAGSEKLCSGTDSIGSGPFKADAYTRGQQRSYTRYADYDWAPKSAGHSGPARL
DSVTIRFITEEATRVGALSSGQVDGAADIPANQIASVSKNPRLTTISKQVPGAVDAFYLNTKSELFSDVRVRKAFQRSLD
LGTIVKSVFQGTTERAWSPLSPTTPNSYDPSLEKTWPYDPKLAGQLLDEAGWTGRDAEGYRTKDGRRLTVFAPIYGEATV
FSQAAQAELKKIGFFLDLHASTDAAEISGLLDGGKYDTVELQWARPDGDILSSFFLSTETSVGGGHNFALVADPQVDEWL
KAAQAEQDPKERAKYYSQVQKWTIDQAVVVPAYIKNATVGVNKKVHGLRLSIATWPEFYPAWVQAD
>Mature_546_residues
MRSRRTARRAGLFAALAVTLATAACGSDGGGSGTADAQPRAGGSVTYAARQEPDCWDPHASAQDVTAFAQRSVFDSLVYQ
TPDGAFEPWLAKSWKISDDGRTYTFELRDDVTFHDGAKLDAEAVKANFDHIMAKDTESQFAAGLLGPYEGVKVTGPQEIQ
VSFSRPYAPLLQVVSTTFLGIASPASLKAGSEKLCSGTDSIGSGPFKADAYTRGQQRSYTRYADYDWAPKSAGHSGPARL
DSVTIRFITEEATRVGALSSGQVDGAADIPANQIASVSKNPRLTTISKQVPGAVDAFYLNTKSELFSDVRVRKAFQRSLD
LGTIVKSVFQGTTERAWSPLSPTTPNSYDPSLEKTWPYDPKLAGQLLDEAGWTGRDAEGYRTKDGRRLTVFAPIYGEATV
FSQAAQAELKKIGFFLDLHASTDAAEISGLLDGGKYDTVELQWARPDGDILSSFFLSTETSVGGGHNFALVADPQVDEWL
KAAQAEQDPKERAKYYSQVQKWTIDQAVVVPAYIKNATVGVNKKVHGLRLSIATWPEFYPAWVQAD

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=477, Percent_Identity=27.6729559748428, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1787052, Length=538, Percent_Identity=24.7211895910781, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787762, Length=455, Percent_Identity=26.1538461538462, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789966, Length=516, Percent_Identity=26.3565891472868, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787551, Length=471, Percent_Identity=22.5053078556263, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082063, Length=496, Percent_Identity=22.5806451612903, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789397, Length=497, Percent_Identity=22.5352112676056, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787495, Length=550, Percent_Identity=22.3636363636364, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59066; Mature: 59066

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRRTARRAGLFAALAVTLATAACGSDGGGSGTADAQPRAGGSVTYAARQEPDCWDPHA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
SAQDVTAFAQRSVFDSLVYQTPDGAFEPWLAKSWKISDDGRTYTFELRDDVTFHDGAKLD
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCCCCEEEEEECCCCEECCCCCCC
AEAVKANFDHIMAKDTESQFAAGLLGPYEGVKVTGPQEIQVSFSRPYAPLLQVVSTTFLG
HHHHHHCHHHHHCCCCHHHHHHHHCCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHH
IASPASLKAGSEKLCSGTDSIGSGPFKADAYTRGQQRSYTRYADYDWAPKSAGHSGPARL
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DSVTIRFITEEATRVGALSSGQVDGAADIPANQIASVSKNPRLTTISKQVPGAVDAFYLN
CEEEEEEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEHHHCCCCEEEEEEC
TKSELFSDVRVRKAFQRSLDLGTIVKSVFQGTTERAWSPLSPTTPNSYDPSLEKTWPYDP
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCH
KLAGQLLDEAGWTGRDAEGYRTKDGRRLTVFAPIYGEATVFSQAAQAELKKIGFFLDLHA
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCEEEEECC
STDAAEISGLLDGGKYDTVELQWARPDGDILSSFFLSTETSVGGGHNFALVADPQVDEWL
CCCHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHH
KAAQAEQDPKERAKYYSQVQKWTIDQAVVVPAYIKNATVGVNKKVHGLRLSIATWPEFYP
HHHHCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCEECCCCCCCEEEEEEECCCCCCC
AWVQAD
CEEECC
>Mature Secondary Structure
MRSRRTARRAGLFAALAVTLATAACGSDGGGSGTADAQPRAGGSVTYAARQEPDCWDPHA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
SAQDVTAFAQRSVFDSLVYQTPDGAFEPWLAKSWKISDDGRTYTFELRDDVTFHDGAKLD
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCCCCEEEEEECCCCEECCCCCCC
AEAVKANFDHIMAKDTESQFAAGLLGPYEGVKVTGPQEIQVSFSRPYAPLLQVVSTTFLG
HHHHHHCHHHHHCCCCHHHHHHHHCCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHH
IASPASLKAGSEKLCSGTDSIGSGPFKADAYTRGQQRSYTRYADYDWAPKSAGHSGPARL
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DSVTIRFITEEATRVGALSSGQVDGAADIPANQIASVSKNPRLTTISKQVPGAVDAFYLN
CEEEEEEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEHHHCCCCEEEEEEC
TKSELFSDVRVRKAFQRSLDLGTIVKSVFQGTTERAWSPLSPTTPNSYDPSLEKTWPYDP
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCH
KLAGQLLDEAGWTGRDAEGYRTKDGRRLTVFAPIYGEATVFSQAAQAELKKIGFFLDLHA
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCEEEEECC
STDAAEISGLLDGGKYDTVELQWARPDGDILSSFFLSTETSVGGGHNFALVADPQVDEWL
CCCHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHH
KAAQAEQDPKERAKYYSQVQKWTIDQAVVVPAYIKNATVGVNKKVHGLRLSIATWPEFYP
HHHHCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCEECCCCCCCEEEEEEECCCCCCC
AWVQAD
CEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]