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The map label for this gene is fadD [C]

Identifier: 158315222

GI number: 158315222

Start: 4051014

End: 4052702

Strand: Reverse

Name: fadD [C]

Synonym: Franean1_3422

Alternate gene names: 158315222

Gene position: 4052702-4051014 (Counterclockwise)

Preceding gene: 158315224

Following gene: 158315221

Centisome position: 45.12

GC content: 68.21

Gene sequence:

>1689_bases
ATGATGAATCTACTCGAGGGGATTGATCGGGCTCGGCTTCAGGGCGGGCGGGTCGTCTTTCTGGACCCGGACGCGGAGGA
CGGTTTGAGCTGGTCTGATTTCTTCGTTCAGGCGGAGCGGGTCGCCGCGTGGTTGCAGGCTGCGCGTGGGATTGGACCGA
GTTCCCGTGTGGTGGTGTTGGCGACGCCGTCAAAGGCGATGGTCACCGCAGTGGTGGCGGTCTGGATGGCCGGAGGCAGT
GTGACTTGCGCCCCAACACCGGCGCGCACCGTCGACCTGCCGACCTACGTGGATCAGACCGGCAAGCGCATCGCCGCGCT
CGGTGACCCGCTCGTCCTGCTCGGTGCACCCTACGAGGAACTTGGGGAGACGCTAGCCACCGACGGGGTCCGGGTCGACC
GTCTCACCGATGCGGTGGCTGCGGAATCGGCCGGCGCGTGGAAGAGACCCGAAATCACTCCTGACGACCCCGCGATCGTG
CAGTTCACCAGCGGTACGACCGCGGCTCCGAAAAGTGTCCTGATCAGTCATGGAAACCTGGTGGCGAACATCGCGGCGAT
CCGAGAACGGATCCGTCATGACGAGGTCCACGGCCGGCTGCTGAGCTGGCTGCCGCTGTCCCACGACATGGGCCTGATTG
GCGCGCTCGCCGTGCAGCTGACGTGCGGGCGTTGCGACGTGCTGTTCGGTACGCCTGCGGACTATCTGGCCTCCCCGTCG
TCGTGGCTGGCCAATGCGGCTCGGTACCGGGCGACGATCCTACTGGGCCCGGCGTCCGCCTACGCAATGGCGGGACGGTT
GCTGGCGGTCGGCCCGCGGCTGGATCTGTCCTCGATCAAGGTCGCACTGTGCGGCGGTGAGCCGATCGAGCCCGCGGCCA
TCGAACGATTCCTCGACGCCGCGGCCCCGCACGGCTTGGACCGGAGCGCTTTCCTGCCCGCCTACGGTCTGGCGGAGGCG
ACCCTGGCGGCCGCGATGCCTCCCACGCCGGGGCTGCGGTTCGACGAGATCGACGCCGACGTCTTTACTCAGCGGCGGGT
CGCCGTTCCCGTCACCGTCCCTGGTGCTCCGGTGCGTCGGCTGGTCCGGCTCGGCCCACCGCTGCCCGGTCTAAAGGTCC
GGCTCGTCGACCCGGAGTCCGGGCAGCCGTGCCCGGAACGATCTGTCGGTGAGATTTACATCTCCGGGGAGTCCGTGACC
TCCGGATACCTCGAAGGCAGCGACGCCGAGCTCGATCGGTGCAGCGGGGCTGGTGAGGCAAGCGAGGCCAGTGGGGTCAG
GCACGTGGCGGACGGCTGGCTGGCGACCGGGGATCTGGGGTATCTCGTCGATGGCGAGCTCGTAGTCTGCGGGCGCGCCA
AGGATCTGATCATCATTGGTGGAAGGAATCTGCATCCCGAGGAAGTCGAGGAGGCCGCCGCCCGGGTTCCTGGGGTTCGG
CCAGGCAATGTCGTCGCCTACCCCACCGCCCGTGACTCCGGCGCCGCCGAAGGGATGGCCGTCGCCGTCGAGACCCGAGG
CGGCCACGAAGAGGCCACGATCCGCGCCAGGGTGACTGCCGCTGTGCTGGCCGCAGTCGGGGTCCGACCGGTCCAAGTTC
ATGTTCTGCCGCCCGGCAGCATCCCCAAGACACCGTCCGGGAAGTTGCAGCGCGCCAAGGCTGCCGGGATGTTCGAGAGC
CGACGATGA

Upstream 100 bases:

>100_bases
GCCTTCTCGCGCGCTGTAGTCGCTGTTTTCTGCCCTGTTCCTGGTGGCCGCCCGCTAGGACTGAACGGGCCGCTGTAATG
TTGGAGGAGGCAAATTTTCA

Downstream 100 bases:

>100_bases
ACAACGGATCAGTGACCCGTCAGGAGACCAGCACCGTCGGTGCCGGGCTCACCCCTGAGGCAGTCCAGGCGGTCGCCACC
GCCGCGGACCGCGTATACGA

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MMNLLEGIDRARLQGGRVVFLDPDAEDGLSWSDFFVQAERVAAWLQAARGIGPSSRVVVLATPSKAMVTAVVAVWMAGGS
VTCAPTPARTVDLPTYVDQTGKRIAALGDPLVLLGAPYEELGETLATDGVRVDRLTDAVAAESAGAWKRPEITPDDPAIV
QFTSGTTAAPKSVLISHGNLVANIAAIRERIRHDEVHGRLLSWLPLSHDMGLIGALAVQLTCGRCDVLFGTPADYLASPS
SWLANAARYRATILLGPASAYAMAGRLLAVGPRLDLSSIKVALCGGEPIEPAAIERFLDAAAPHGLDRSAFLPAYGLAEA
TLAAAMPPTPGLRFDEIDADVFTQRRVAVPVTVPGAPVRRLVRLGPPLPGLKVRLVDPESGQPCPERSVGEIYISGESVT
SGYLEGSDAELDRCSGAGEASEASGVRHVADGWLATGDLGYLVDGELVVCGRAKDLIIIGGRNLHPEEVEEAAARVPGVR
PGNVVAYPTARDSGAAEGMAVAVETRGGHEEATIRARVTAAVLAAVGVRPVQVHVLPPGSIPKTPSGKLQRAKAAGMFES
RR

Sequences:

>Translated_562_residues
MMNLLEGIDRARLQGGRVVFLDPDAEDGLSWSDFFVQAERVAAWLQAARGIGPSSRVVVLATPSKAMVTAVVAVWMAGGS
VTCAPTPARTVDLPTYVDQTGKRIAALGDPLVLLGAPYEELGETLATDGVRVDRLTDAVAAESAGAWKRPEITPDDPAIV
QFTSGTTAAPKSVLISHGNLVANIAAIRERIRHDEVHGRLLSWLPLSHDMGLIGALAVQLTCGRCDVLFGTPADYLASPS
SWLANAARYRATILLGPASAYAMAGRLLAVGPRLDLSSIKVALCGGEPIEPAAIERFLDAAAPHGLDRSAFLPAYGLAEA
TLAAAMPPTPGLRFDEIDADVFTQRRVAVPVTVPGAPVRRLVRLGPPLPGLKVRLVDPESGQPCPERSVGEIYISGESVT
SGYLEGSDAELDRCSGAGEASEASGVRHVADGWLATGDLGYLVDGELVVCGRAKDLIIIGGRNLHPEEVEEAAARVPGVR
PGNVVAYPTARDSGAAEGMAVAVETRGGHEEATIRARVTAAVLAAVGVRPVQVHVLPPGSIPKTPSGKLQRAKAAGMFES
RR
>Mature_562_residues
MMNLLEGIDRARLQGGRVVFLDPDAEDGLSWSDFFVQAERVAAWLQAARGIGPSSRVVVLATPSKAMVTAVVAVWMAGGS
VTCAPTPARTVDLPTYVDQTGKRIAALGDPLVLLGAPYEELGETLATDGVRVDRLTDAVAAESAGAWKRPEITPDDPAIV
QFTSGTTAAPKSVLISHGNLVANIAAIRERIRHDEVHGRLLSWLPLSHDMGLIGALAVQLTCGRCDVLFGTPADYLASPS
SWLANAARYRATILLGPASAYAMAGRLLAVGPRLDLSSIKVALCGGEPIEPAAIERFLDAAAPHGLDRSAFLPAYGLAEA
TLAAAMPPTPGLRFDEIDADVFTQRRVAVPVTVPGAPVRRLVRLGPPLPGLKVRLVDPESGQPCPERSVGEIYISGESVT
SGYLEGSDAELDRCSGAGEASEASGVRHVADGWLATGDLGYLVDGELVVCGRAKDLIIIGGRNLHPEEVEEAAARVPGVR
PGNVVAYPTARDSGAAEGMAVAVETRGGHEEATIRARVTAAVLAAVGVRPVQVHVLPPGSIPKTPSGKLQRAKAAGMFES
RR

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=425, Percent_Identity=24.4705882352941, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1788107, Length=398, Percent_Identity=24.3718592964824, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789201, Length=354, Percent_Identity=26.5536723163842, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=579, Percent_Identity=23.3160621761658, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=406, Percent_Identity=23.3990147783251, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24581924, Length=424, Percent_Identity=26.6509433962264, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859661, Length=446, Percent_Identity=24.8878923766816, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922652, Length=435, Percent_Identity=26.2068965517241, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355181, Length=412, Percent_Identity=22.8155339805825, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58737; Mature: 58737

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNLLEGIDRARLQGGRVVFLDPDAEDGLSWSDFFVQAERVAAWLQAARGIGPSSRVVVL
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
ATPSKAMVTAVVAVWMAGGSVTCAPTPARTVDLPTYVDQTGKRIAALGDPLVLLGAPYEE
ECCCHHHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHCCCEEEECCCCEEEEECCHHH
LGETLATDGVRVDRLTDAVAAESAGAWKRPEITPDDPAIVQFTSGTTAAPKSVLISHGNL
HHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCH
VANIAAIRERIRHDEVHGRLLSWLPLSHDMGLIGALAVQLTCGRCDVLFGTPADYLASPS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEECCCEEEEECCCHHHHCCCH
SWLANAARYRATILLGPASAYAMAGRLLAVGPRLDLSSIKVALCGGEPIEPAAIERFLDA
HHHHHHHHEEEEEEECCHHHHHHHCEEEEECCCCCHHHEEEEEECCCCCCHHHHHHHHHH
AAPHGLDRSAFLPAYGLAEATLAAAMPPTPGLRFDEIDADVFTQRRVAVPVTVPGAPVRR
HCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEECCCCHHHHCCCEEEEEEECCCHHHHH
LVRLGPPLPGLKVRLVDPESGQPCPERSVGEIYISGESVTSGYLEGSDAELDRCSGAGEA
HHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCHHHCCCCCCC
SEASGVRHVADGWLATGDLGYLVDGELVVCGRAKDLIIIGGRNLHPEEVEEAAARVPGVR
HHHCCCHHHHCCEEEECCCCEEECCCEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCC
PGNVVAYPTARDSGAAEGMAVAVETRGGHEEATIRARVTAAVLAAVGVRPVQVHVLPPGS
CCCEEEECCCCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHCCCEEEEEEECCCC
IPKTPSGKLQRAKAAGMFESRR
CCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMNLLEGIDRARLQGGRVVFLDPDAEDGLSWSDFFVQAERVAAWLQAARGIGPSSRVVVL
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
ATPSKAMVTAVVAVWMAGGSVTCAPTPARTVDLPTYVDQTGKRIAALGDPLVLLGAPYEE
ECCCHHHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHCCCEEEECCCCEEEEECCHHH
LGETLATDGVRVDRLTDAVAAESAGAWKRPEITPDDPAIVQFTSGTTAAPKSVLISHGNL
HHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCH
VANIAAIRERIRHDEVHGRLLSWLPLSHDMGLIGALAVQLTCGRCDVLFGTPADYLASPS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEECCCEEEEECCCHHHHCCCH
SWLANAARYRATILLGPASAYAMAGRLLAVGPRLDLSSIKVALCGGEPIEPAAIERFLDA
HHHHHHHHEEEEEEECCHHHHHHHCEEEEECCCCCHHHEEEEEECCCCCCHHHHHHHHHH
AAPHGLDRSAFLPAYGLAEATLAAAMPPTPGLRFDEIDADVFTQRRVAVPVTVPGAPVRR
HCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEECCCCHHHHCCCEEEEEEECCCHHHHH
LVRLGPPLPGLKVRLVDPESGQPCPERSVGEIYISGESVTSGYLEGSDAELDRCSGAGEA
HHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCHHHCCCCCCC
SEASGVRHVADGWLATGDLGYLVDGELVVCGRAKDLIIIGGRNLHPEEVEEAAARVPGVR
HHHCCCHHHHCCEEEECCCCEEECCCEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCC
PGNVVAYPTARDSGAAEGMAVAVETRGGHEEATIRARVTAAVLAAVGVRPVQVHVLPPGS
CCCEEEECCCCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHCCCEEEEEEECCCC
IPKTPSGKLQRAKAAGMFESRR
CCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA