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The map label for this gene is lcfB [H]

Identifier: 158315199

GI number: 158315199

Start: 4029790

End: 4031358

Strand: Reverse

Name: lcfB [H]

Synonym: Franean1_3399

Alternate gene names: 158315199

Gene position: 4031358-4029790 (Counterclockwise)

Preceding gene: 158315200

Following gene: 158315198

Centisome position: 44.88

GC content: 70.68

Gene sequence:

>1569_bases
ATGTACCCACCAGACTTCGCGACGCTGACCCCTGACAAGCCCGCAGTGATCCTCGCCGGTGGCCCCCGCGGGCAGGAACG
GATCCTGACCTACCGTGAGCTCGTCGAGGGCTCCAACCGGCTGGCCCGGTTACTCGTCGACTCGGGCCTGCGCCCGGGTG
ACCGCCTCGCGATTCTCGCCGAGAACCACCTGCGCTATTTCGAGCTGGTCTGGGCCGGCCTCAACTGCGGCCTCTACATC
ACCCCGGTGAACTCGCATCTCACCCCGCCCGAGGTCGCGTACCTGATCAACGACAGCGGGGCCAGGGCGTTGATCAGCAG
CCGGGCGCTCGCGGCGGTCGCCGAGGCCGTCGTCCCTGAGACCCCCGGGGTCGTCCGGCGCCTCATGCTCGACGGGGGCT
CCGAGCACTACGAGGATCTCGACGCCGCCACCGCCGGCTTCTCCGCCGAGCCCCGGGACGACGAGATCCGCGGCACGTTC
ATGCTCTACAGCTCCGGGACGACCGGCCGGCCGAAGGGAATCCAGTTCCCGCTGCCCGACTGGCCCGCCAGCGAGGGCGA
CGCCCCGCTGCTTCCCGGCGCCCGCGGGGCCTTCGGCTTCAACGCCGAAGCGGTCTACATCTCCCCCGCGCCGCTCTACC
ACGCAGCCCCGCTACGGGTGTCCGCGCTGATGCACAGCGTCGGCGGCACGGTCGTCGTCCTCCCGAAGTTCGACGCCGAG
GGGGCCCTGCACGCGATCGAACGGTACCGGGTGACCACCTCCCAGTGGGTCCCGACGATGTTCGTCCGGATGCTCAAGCT
GCCACCCGAGGTCCGCGCCCGCTACGACCTGTCGAGCCTGCGGATCGCCGTCCACGCGGCCGCGCCGTGCCCGGTCGAGG
TCAAGCGCCAGATGATCGAGTGGTGGGGCCCGATCATCTTCGAGTACTACTCCGGCTCGGAGAACGTCGGCAGCACCGGG
CTGACCAGCGAGGAGTGGCTGGCGCACCCGGGTTCGGTCGGCCGTGCGCAGGGCGGCGTGCTGCACATCTGCGGCGAGGA
CGGCGCGGAGCTCCCGGCCGGCCAGGACGGAGCCGTCTACTTCGAGGCCAAGGGCGCGGGCTTCAATTACCACAACGACC
CGGACCGCACCAGAGCCGTCAGCCATCCCGCCCACCCCGGCTGGCGCACCCTGGGCGACATCGGCCACGTCGACGAGGAC
GGCTACCTCTACCTCAGTGACCGGAAGGACTTCACGATCATCGCGGGCGGGGTGAACATCTACCCGCGCGAGATCGAGGA
CGTCCTGGTGCTGCACGACGAGGTCGTGGACGTCGCCGTGTTCGGCGTGCCGCACCCCGAGCTCGGCGAGCAGGTCAAGG
CCGTCGTCCAGCCGGTGCGGATGGCCGACGCCGGCGACGGGCTGGCCGCGCGGCTGCTGGAGCACTGCCGCACCAGGCTC
GCGCCGTTCAAGTGGCCGCGGTCGATCGACTTCGTGCCCGAACTCCCCCGCCTGGACAACGGCAAGCTCTACAAGAAGCC
GCTGCGCGACGCCTACTGGGCCACCGAGGCCTCCACCGTCGCCCGTTAG

Upstream 100 bases:

>100_bases
GCGCGGGCCATCTATCTCCACCGCCAAGAATCTGGTATCACCCATTGAAAGAGCTACGTTTCCCGTATGGCGAACCGTTG
TTCTCTCCCTGGAGGACCAG

Downstream 100 bases:

>100_bases
CCGCCGGGAACCAGTGAGAGGACTGCAATGTTTCGCAGAGAGAAGTTACTCACCACCGTCATCGCCTGTTGTGTGGCGCT
CGCCGTGGGCGCCTGCGGCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MYPPDFATLTPDKPAVILAGGPRGQERILTYRELVEGSNRLARLLVDSGLRPGDRLAILAENHLRYFELVWAGLNCGLYI
TPVNSHLTPPEVAYLINDSGARALISSRALAAVAEAVVPETPGVVRRLMLDGGSEHYEDLDAATAGFSAEPRDDEIRGTF
MLYSSGTTGRPKGIQFPLPDWPASEGDAPLLPGARGAFGFNAEAVYISPAPLYHAAPLRVSALMHSVGGTVVVLPKFDAE
GALHAIERYRVTTSQWVPTMFVRMLKLPPEVRARYDLSSLRIAVHAAAPCPVEVKRQMIEWWGPIIFEYYSGSENVGSTG
LTSEEWLAHPGSVGRAQGGVLHICGEDGAELPAGQDGAVYFEAKGAGFNYHNDPDRTRAVSHPAHPGWRTLGDIGHVDED
GYLYLSDRKDFTIIAGGVNIYPREIEDVLVLHDEVVDVAVFGVPHPELGEQVKAVVQPVRMADAGDGLAARLLEHCRTRL
APFKWPRSIDFVPELPRLDNGKLYKKPLRDAYWATEASTVAR

Sequences:

>Translated_522_residues
MYPPDFATLTPDKPAVILAGGPRGQERILTYRELVEGSNRLARLLVDSGLRPGDRLAILAENHLRYFELVWAGLNCGLYI
TPVNSHLTPPEVAYLINDSGARALISSRALAAVAEAVVPETPGVVRRLMLDGGSEHYEDLDAATAGFSAEPRDDEIRGTF
MLYSSGTTGRPKGIQFPLPDWPASEGDAPLLPGARGAFGFNAEAVYISPAPLYHAAPLRVSALMHSVGGTVVVLPKFDAE
GALHAIERYRVTTSQWVPTMFVRMLKLPPEVRARYDLSSLRIAVHAAAPCPVEVKRQMIEWWGPIIFEYYSGSENVGSTG
LTSEEWLAHPGSVGRAQGGVLHICGEDGAELPAGQDGAVYFEAKGAGFNYHNDPDRTRAVSHPAHPGWRTLGDIGHVDED
GYLYLSDRKDFTIIAGGVNIYPREIEDVLVLHDEVVDVAVFGVPHPELGEQVKAVVQPVRMADAGDGLAARLLEHCRTRL
APFKWPRSIDFVPELPRLDNGKLYKKPLRDAYWATEASTVAR
>Mature_522_residues
MYPPDFATLTPDKPAVILAGGPRGQERILTYRELVEGSNRLARLLVDSGLRPGDRLAILAENHLRYFELVWAGLNCGLYI
TPVNSHLTPPEVAYLINDSGARALISSRALAAVAEAVVPETPGVVRRLMLDGGSEHYEDLDAATAGFSAEPRDDEIRGTF
MLYSSGTTGRPKGIQFPLPDWPASEGDAPLLPGARGAFGFNAEAVYISPAPLYHAAPLRVSALMHSVGGTVVVLPKFDAE
GALHAIERYRVTTSQWVPTMFVRMLKLPPEVRARYDLSSLRIAVHAAAPCPVEVKRQMIEWWGPIIFEYYSGSENVGSTG
LTSEEWLAHPGSVGRAQGGVLHICGEDGAELPAGQDGAVYFEAKGAGFNYHNDPDRTRAVSHPAHPGWRTLGDIGHVDED
GYLYLSDRKDFTIIAGGVNIYPREIEDVLVLHDEVVDVAVFGVPHPELGEQVKAVVQPVRMADAGDGLAARLLEHCRTRL
APFKWPRSIDFVPELPRLDNGKLYKKPLRDAYWATEASTVAR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=530, Percent_Identity=26.7924528301887, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI115511026, Length=525, Percent_Identity=26.4761904761905, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI42544132, Length=510, Percent_Identity=26.2745098039216, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI157311624, Length=532, Percent_Identity=26.8796992481203, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI157311622, Length=532, Percent_Identity=26.8796992481203, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI58082049, Length=533, Percent_Identity=26.8292682926829, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI122937307, Length=523, Percent_Identity=28.2982791586998, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI156151445, Length=519, Percent_Identity=26.0115606936416, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI187761345, Length=533, Percent_Identity=26.6416510318949, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI187761343, Length=533, Percent_Identity=26.6416510318949, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI227499619, Length=529, Percent_Identity=24.3856332703214, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI8923896, Length=563, Percent_Identity=23.6234458259325, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI13375727, Length=553, Percent_Identity=22.4231464737794, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI253970489, Length=575, Percent_Identity=22.9565217391304, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=534, Percent_Identity=26.5917602996255, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI221142682, Length=533, Percent_Identity=24.765478424015, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI145693145, Length=529, Percent_Identity=24.5746691871456, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786810, Length=520, Percent_Identity=26.1538461538462, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=528, Percent_Identity=26.7045454545455, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=510, Percent_Identity=27.2549019607843, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560308, Length=540, Percent_Identity=28.1481481481481, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=26.953125, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=550, Percent_Identity=26.1818181818182, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=530, Percent_Identity=26.0377358490566, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=530, Percent_Identity=26.0377358490566, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=539, Percent_Identity=25.2319109461967, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=528, Percent_Identity=25.7575757575758, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=523, Percent_Identity=23.3269598470363, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=380, Percent_Identity=24.4736842105263, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=378, Percent_Identity=25.3968253968254, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21355181, Length=488, Percent_Identity=28.8934426229508, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24581924, Length=537, Percent_Identity=24.2085661080074, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI18859661, Length=525, Percent_Identity=23.8095238095238, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI62472339, Length=546, Percent_Identity=25.0915750915751, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24656500, Length=524, Percent_Identity=25, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24667955, Length=550, Percent_Identity=25.0909090909091, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI19922652, Length=503, Percent_Identity=23.6580516898608, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281365686, Length=335, Percent_Identity=25.9701492537313, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648260, Length=509, Percent_Identity=24.950884086444, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20130357, Length=506, Percent_Identity=23.9130434782609, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21358303, Length=508, Percent_Identity=23.0314960629921, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355225, Length=376, Percent_Identity=25.2659574468085, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550496, Length=537, Percent_Identity=24.7672253258845, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653035, Length=500, Percent_Identity=24.2, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45552801, Length=536, Percent_Identity=24.8134328358209, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356441, Length=352, Percent_Identity=25, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648253, Length=494, Percent_Identity=23.4817813765182, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648255, Length=348, Percent_Identity=26.1494252873563, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583465, Length=510, Percent_Identity=24.1176470588235, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583463, Length=510, Percent_Identity=24.1176470588235, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24667959, Length=374, Percent_Identity=25.4010695187166, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281364798, Length=510, Percent_Identity=24.1176470588235, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356947, Length=505, Percent_Identity=24.1584158415842, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648257, Length=512, Percent_Identity=23.828125, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161076582, Length=507, Percent_Identity=22.2879684418146, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56917; Mature: 56917

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPPDFATLTPDKPAVILAGGPRGQERILTYRELVEGSNRLARLLVDSGLRPGDRLAILA
CCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEE
ENHLRYFELVWAGLNCGLYITPVNSHLTPPEVAYLINDSGARALISSRALAAVAEAVVPE
CCCCEEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCC
TPGVVRRLMLDGGSEHYEDLDAATAGFSAEPRDDEIRGTFMLYSSGTTGRPKGIQFPLPD
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
WPASEGDAPLLPGARGAFGFNAEAVYISPAPLYHAAPLRVSALMHSVGGTVVVLPKFDAE
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
GALHAIERYRVTTSQWVPTMFVRMLKLPPEVRARYDLSSLRIAVHAAAPCPVEVKRQMIE
HHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHH
WWGPIIFEYYSGSENVGSTGLTSEEWLAHPGSVGRAQGGVLHICGEDGAELPAGQDGAVY
HHCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEE
FEAKGAGFNYHNDPDRTRAVSHPAHPGWRTLGDIGHVDEDGYLYLSDRKDFTIIAGGVNI
EEECCCCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCEEEEECCCEE
YPREIEDVLVLHDEVVDVAVFGVPHPELGEQVKAVVQPVRMADAGDGLAARLLEHCRTRL
CCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
APFKWPRSIDFVPELPRLDNGKLYKKPLRDAYWATEASTVAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MYPPDFATLTPDKPAVILAGGPRGQERILTYRELVEGSNRLARLLVDSGLRPGDRLAILA
CCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEE
ENHLRYFELVWAGLNCGLYITPVNSHLTPPEVAYLINDSGARALISSRALAAVAEAVVPE
CCCCEEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCC
TPGVVRRLMLDGGSEHYEDLDAATAGFSAEPRDDEIRGTFMLYSSGTTGRPKGIQFPLPD
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
WPASEGDAPLLPGARGAFGFNAEAVYISPAPLYHAAPLRVSALMHSVGGTVVVLPKFDAE
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
GALHAIERYRVTTSQWVPTMFVRMLKLPPEVRARYDLSSLRIAVHAAAPCPVEVKRQMIE
HHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHH
WWGPIIFEYYSGSENVGSTGLTSEEWLAHPGSVGRAQGGVLHICGEDGAELPAGQDGAVY
HHCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEE
FEAKGAGFNYHNDPDRTRAVSHPAHPGWRTLGDIGHVDEDGYLYLSDRKDFTIIAGGVNI
EEECCCCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCEEEEECCCEE
YPREIEDVLVLHDEVVDVAVFGVPHPELGEQVKAVVQPVRMADAGDGLAARLLEHCRTRL
CCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
APFKWPRSIDFVPELPRLDNGKLYKKPLRDAYWATEASTVAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]