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The map label for this gene is gsiB [H]

Identifier: 158315198

GI number: 158315198

Start: 4028176

End: 4029762

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_3398

Alternate gene names: 158315198

Gene position: 4029762-4028176 (Counterclockwise)

Preceding gene: 158315199

Following gene: 158315192

Centisome position: 44.86

GC content: 68.24

Gene sequence:

>1587_bases
ATGTTTCGCAGAGAGAAGTTACTCACCACCGTCATCGCCTGTTGTGTGGCGCTCGCCGTGGGCGCCTGCGGCGGCGGTGA
CGGCTCGTCCACGACGACCGGCACCACCACCACCGCCACCGGACCCACCGGCGACCCGGTCGCCGGCGGCTCGCTGCGGG
TCATGCAGCTCCGCGAGCCCCGTTCGATGGATCCGGTCACCATGACGAACGCGTGGCAGGTCAACGCGTTCGTGGGCAAC
GCCCTGTACGGCACGTTGATGATCAACGATGAGCACACCAACGAGATCAAGTACACGATGGCGGAGTCGTTCGCCACCAC
CGACAAGGGCGCCACGTTCGCGCTGAAACTGCGACCGGGCCTGAAGTTCACCGACGGCACCCCGCTCGACGCCGCCGCGG
TGAAGTTCAACTGGGATCGCCATCGGGATCCGGCGATGGCCTCGCCCTACCTGCCGGAGGCGTCCCTCATCGCCTCCACC
GACGTCGTGGACGCCACGACCCTCAAGGTGACGATGACCGAGCCGGTGCCCAGCTTCCCGGCCGCGCTGCTCACCACGTC
CATGAACTGGATCGCCTCCCCCACCGCCCTGCAGAAGGGCAAGGCGTCCTTCGACGCGAAACCGGTCGGCGCGGGGCCGT
ACACCGTGCGGACCTGGACCCGCCAGGACCGCATCGAGCTGGCGAAGAACCCCGGCTACTGGGACGCCCCCCGGCCCTAC
CTCGACAGCATCACCATCCGCACGTCGGACGACTCGGGCCAGCGGTACAACACGCTGATCAGCGGCGGCGCCGACGTGGT
CATCGACACGAACGCGGACAGCGTCGCCAAGGCCGGGAAGGCCGGCTACTCGACGGACGTCGCGCCGCTGAGCGGCGGCC
TGTTCCTCGCGATGAACATGCGCAGGGCGCCGTTCGACGACCTGCGGGCCCGGCAGGCGATCGCGGCCGCCGTCGACCTG
GACGCGCTGAACCTGGCCGTGTACAACGGCGCCGCCCAGACCGCCGACACGCTGTTCACCAAGTCCTCGCCGTTCTACTC
CGACAAGCCGCTGCGGACGTACGACCGGGCGAAGGCGCAGCAGCTGTTCGACGAGCTGGCCGCCGACGGCAAGCCTGTCA
CCTTCACGTTCACCAGCTTCGCCTCGTCGGAGATCAGGGACACGACCGAGAACGTCCAGGCCCAGCTCAGCTCGTACAAG
AACGTCAAGGTTCAGGTGCGGGTGGTCGACTTCTCCGAGTATCCCAAGATCCAGGCGAGCAACGACTTCGACATGGTCGT
CTGGTCGGCGAACTTCATCGACCCGGACCCACGGATGTGGACGGCGTTCCGCAGCGACTCCCGCGGCAACTTCCCGGGCG
TCAAGGACGAGCGGATCGACGCCGCCCTGAAGGCGGGCCGCACGGCGACGACCGAACAGGAGCGCAAGGCCGCGTACGCC
ACCCTCCAGGAGCGGCTGACCGCGCTGGTTCCCGCGGTCTTCACCGTCCGGGTGAACCCGAGCGTCATGGCCGCCAGGAA
CGTCGGCGGCGTCACCCAGTACGGCCTCGGATCACTCCTGCCCGAGCCCCTGTGGCTCCAGCCATGA

Upstream 100 bases:

>100_bases
CAACGGCAAGCTCTACAAGAAGCCGCTGCGCGACGCCTACTGGGCCACCGAGGCCTCCACCGTCGCCCGTTAGCCGCCGG
GAACCAGTGAGAGGACTGCA

Downstream 100 bases:

>100_bases
CCTGACCTCAGACGCTTGAGGTGGATGTCGCCGCGGGCTGGGCCGCCTGCGGCGACGTCCGGCGTGGTTCCGGGAGCGTG
ACGGTCGACTTCGACGCCGC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MFRREKLLTTVIACCVALAVGACGGGDGSSTTTGTTTTATGPTGDPVAGGSLRVMQLREPRSMDPVTMTNAWQVNAFVGN
ALYGTLMINDEHTNEIKYTMAESFATTDKGATFALKLRPGLKFTDGTPLDAAAVKFNWDRHRDPAMASPYLPEASLIAST
DVVDATTLKVTMTEPVPSFPAALLTTSMNWIASPTALQKGKASFDAKPVGAGPYTVRTWTRQDRIELAKNPGYWDAPRPY
LDSITIRTSDDSGQRYNTLISGGADVVIDTNADSVAKAGKAGYSTDVAPLSGGLFLAMNMRRAPFDDLRARQAIAAAVDL
DALNLAVYNGAAQTADTLFTKSSPFYSDKPLRTYDRAKAQQLFDELAADGKPVTFTFTSFASSEIRDTTENVQAQLSSYK
NVKVQVRVVDFSEYPKIQASNDFDMVVWSANFIDPDPRMWTAFRSDSRGNFPGVKDERIDAALKAGRTATTEQERKAAYA
TLQERLTALVPAVFTVRVNPSVMAARNVGGVTQYGLGSLLPEPLWLQP

Sequences:

>Translated_528_residues
MFRREKLLTTVIACCVALAVGACGGGDGSSTTTGTTTTATGPTGDPVAGGSLRVMQLREPRSMDPVTMTNAWQVNAFVGN
ALYGTLMINDEHTNEIKYTMAESFATTDKGATFALKLRPGLKFTDGTPLDAAAVKFNWDRHRDPAMASPYLPEASLIAST
DVVDATTLKVTMTEPVPSFPAALLTTSMNWIASPTALQKGKASFDAKPVGAGPYTVRTWTRQDRIELAKNPGYWDAPRPY
LDSITIRTSDDSGQRYNTLISGGADVVIDTNADSVAKAGKAGYSTDVAPLSGGLFLAMNMRRAPFDDLRARQAIAAAVDL
DALNLAVYNGAAQTADTLFTKSSPFYSDKPLRTYDRAKAQQLFDELAADGKPVTFTFTSFASSEIRDTTENVQAQLSSYK
NVKVQVRVVDFSEYPKIQASNDFDMVVWSANFIDPDPRMWTAFRSDSRGNFPGVKDERIDAALKAGRTATTEQERKAAYA
TLQERLTALVPAVFTVRVNPSVMAARNVGGVTQYGLGSLLPEPLWLQP
>Mature_528_residues
MFRREKLLTTVIACCVALAVGACGGGDGSSTTTGTTTTATGPTGDPVAGGSLRVMQLREPRSMDPVTMTNAWQVNAFVGN
ALYGTLMINDEHTNEIKYTMAESFATTDKGATFALKLRPGLKFTDGTPLDAAAVKFNWDRHRDPAMASPYLPEASLIAST
DVVDATTLKVTMTEPVPSFPAALLTTSMNWIASPTALQKGKASFDAKPVGAGPYTVRTWTRQDRIELAKNPGYWDAPRPY
LDSITIRTSDDSGQRYNTLISGGADVVIDTNADSVAKAGKAGYSTDVAPLSGGLFLAMNMRRAPFDDLRARQAIAAAVDL
DALNLAVYNGAAQTADTLFTKSSPFYSDKPLRTYDRAKAQQLFDELAADGKPVTFTFTSFASSEIRDTTENVQAQLSSYK
NVKVQVRVVDFSEYPKIQASNDFDMVVWSANFIDPDPRMWTAFRSDSRGNFPGVKDERIDAALKAGRTATTEQERKAAYA
TLQERLTALVPAVFTVRVNPSVMAARNVGGVTQYGLGSLLPEPLWLQP

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=338, Percent_Identity=32.2485207100592, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789966, Length=462, Percent_Identity=26.1904761904762, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787495, Length=476, Percent_Identity=23.5294117647059, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789397, Length=488, Percent_Identity=23.7704918032787, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787762, Length=475, Percent_Identity=23.3684210526316, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789887, Length=469, Percent_Identity=23.6673773987207, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081878, Length=523, Percent_Identity=21.6061185468451, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787551, Length=446, Percent_Identity=23.3183856502242, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57010; Mature: 57010

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRREKLLTTVIACCVALAVGACGGGDGSSTTTGTTTTATGPTGDPVAGGSLRVMQLREP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEECCC
RSMDPVTMTNAWQVNAFVGNALYGTLMINDEHTNEIKYTMAESFATTDKGATFALKLRPG
CCCCCEEECCCEEEEEEECCEEEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCC
LKFTDGTPLDAAAVKFNWDRHRDPAMASPYLPEASLIASTDVVDATTLKVTMTEPVPSFP
CEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHEECCCCEEEEEEEEEECCCCCCCC
AALLTTSMNWIASPTALQKGKASFDAKPVGAGPYTVRTWTRQDRIELAKNPGYWDAPRPY
HHHHHHCHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCCCCCCCCCC
LDSITIRTSDDSGQRYNTLISGGADVVIDTNADSVAKAGKAGYSTDVAPLSGGLFLAMNM
CCEEEEEECCCCCCHHHHEECCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCEEEEEEC
RRAPFDDLRARQAIAAAVDLDALNLAVYNGAAQTADTLFTKSSPFYSDKPLRTYDRAKAQ
CCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHEECCCCCCCCCCCCHHHHHHHH
QLFDELAADGKPVTFTFTSFASSEIRDTTENVQAQLSSYKNVKVQVRVVDFSEYPKIQAS
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEECC
NDFDMVVWSANFIDPDPRMWTAFRSDSRGNFPGVKDERIDAALKAGRTATTEQERKAAYA
CCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
TLQERLTALVPAVFTVRVNPSVMAARNVGGVTQYGLGSLLPEPLWLQP
HHHHHHHHHHCEEEEEEECCCEEEECCCCCCHHHCHHHHCCCCCCCCC
>Mature Secondary Structure
MFRREKLLTTVIACCVALAVGACGGGDGSSTTTGTTTTATGPTGDPVAGGSLRVMQLREP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEECCC
RSMDPVTMTNAWQVNAFVGNALYGTLMINDEHTNEIKYTMAESFATTDKGATFALKLRPG
CCCCCEEECCCEEEEEEECCEEEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCC
LKFTDGTPLDAAAVKFNWDRHRDPAMASPYLPEASLIASTDVVDATTLKVTMTEPVPSFP
CEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHEECCCCEEEEEEEEEECCCCCCCC
AALLTTSMNWIASPTALQKGKASFDAKPVGAGPYTVRTWTRQDRIELAKNPGYWDAPRPY
HHHHHHCHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCCCCCCCCCC
LDSITIRTSDDSGQRYNTLISGGADVVIDTNADSVAKAGKAGYSTDVAPLSGGLFLAMNM
CCEEEEEECCCCCCHHHHEECCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCEEEEEEC
RRAPFDDLRARQAIAAAVDLDALNLAVYNGAAQTADTLFTKSSPFYSDKPLRTYDRAKAQ
CCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHEECCCCCCCCCCCCHHHHHHHH
QLFDELAADGKPVTFTFTSFASSEIRDTTENVQAQLSSYKNVKVQVRVVDFSEYPKIQAS
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEECC
NDFDMVVWSANFIDPDPRMWTAFRSDSRGNFPGVKDERIDAALKAGRTATTEQERKAAYA
CCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
TLQERLTALVPAVFTVRVNPSVMAARNVGGVTQYGLGSLLPEPLWLQP
HHHHHHHHHHCEEEEEEECCCEEEECCCCCCHHHCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA