Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 158315142

GI number: 158315142

Start: 3953925

End: 3955478

Strand: Direct

Name: emrB [H]

Synonym: Franean1_3341

Alternate gene names: 158315142

Gene position: 3953925-3955478 (Clockwise)

Preceding gene: 158315139

Following gene: 158315143

Centisome position: 44.02

GC content: 72.46

Gene sequence:

>1554_bases
ATGTCGGCCGAAGCTCGGGACGCGGACCGGCTCGACCCGCCGCTGCGACGCCTGATCGCCGTGGTTCTGCTCGGCGGGAT
CATGGGAATCCTCGACGGGTCCGTCGTCGCCGTCGGTGTCGACACCCTCGCCGCGCGGTTCCACGCCTCGCTGAGCACGA
TCGGATGGGTCTCGACCGGATACCTCCTGGCCCTCACCATTGCCATCCCGATCACCACCTGGGCTGTCGACAGGTTCGGC
GCGCGCCGGCTGTGGCTGACCGCCCTCGTCGTCTTCCTGTCCGCCTCCCTGGCCTCCGGTCTCGCCTGGAACATCACCAG
CCTGATCGTCTTCCGGGTCATCCAGGGCCTTGCCGCCGGCGTGCTCGACCCGCTGGTGCTGACCCTGCTCGCCCGCGCCG
CGGGACCACGCCGCGCCGGCCGGGTCATGGGGTTGATGGGCATGGTGCTCTCGGCCGGCCCGGTGCTCGGCCTGATCGTC
GGTGGGATCGTCCTGGAGAGCGCCTCCTGGCGATGGATGTTCCTGATCAACCTGCCGGTCGGGGTCGTGGCCCTGCTCGG
CGCAGCCCGTGCGGTACCTGCCGACCCGCCACGCCAGACGCCCACCAGCCGCCTCGACGTCCTCGGTGTCGCGCTGATCG
GCCCGGGGTTCGCCGCCGTGGTCCTCGCGTTGACGCAGGCGGCTGACCGCACCGCCGTCGTCGCCTGGCAGGTGCTCGTC
CCGCTCGTCGCGGCGGTGACGCTGCTCGCCGGCTACGCCGTGCACGCCCTGCGTGGACGTCCGCACCGGAGCACGCCACC
CCTGATCGACCTGCGCCTGTTCGCCAGTCCTGGATTCGCCGCGAGCGTGGCCGTCATGACTCTGGTGGGCCTGACGATGT
TCGCGAACCTCTTCGTGCTCCCGCTCTACTACCAGCAGCAGCATGGACGGGGCGCGCTCGGGGCCGGGCTGCTGGTGGCA
CCCTTCGCGGTGGCCGCCGTCATCTGCATGCCCCTGTCCGGGCGGCTGTCCGACCGGCTCGGCCCTCGGGCCCTCGTCCG
GGGCGGGGCGGTTGTCGCGGTACTCGGCCAACTGGCGTTCACCGGGATCGGCGCGCACACCAACGAGGTGTGGCCCGCGC
TCGCCGCGTTCGTGGTCGGCGCGGGGCTGAGCTTCGTCGGCGCGCCCACCATGGGATCCCTGTACCGGACGCTGCCTCCG
GAGTCCGTGTCCCAGGGCAGCTCCGTGCTCTACATCCTCAACCAGCTCGGCGCGGCCATCGGCATCGCCGTCGTCACCCT
GATCCTGGCGACCGTTGGGCCGGAGAACACCCGCGGGATGGCGGGGTTCCACGGTGTCCACTGGTTCGCGCTGGCGGCCG
TCGCCGTCATCCTCGCCATGAGCGCGTTCCTCCCCCGGCGAGACGCGACGACCGAGGCGGCGGTCCCGGCCGCAGGTGCC
GCGACCGCGACCGCGACCGCGACCGCGACCACGTCGACGTCGACGTCGACGTCGACGTCCGGGACGGTCCCGCCAGCCCT
GTCCGCCGCGACCGCCCAGGAGGGCACCCGATGA

Upstream 100 bases:

>100_bases
CCGTAGCTGCCGGCCGCCGTCGGTCTCCCGCTGCCGTGGGAACGGATACGAGCGCTAGACTGCACTCGCACGTGCAAAAC
TATAGACACTCGGAGGCATT

Downstream 100 bases:

>100_bases
GGACCATCGTGATCACCGGAGGGACCGACGGGATCGGCCAGTCCCTCGCCCGCACCCTCCTGGGCCGTGGCGACCAGGTC
GTGGTCGTCGGCCGCAACGC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSAEARDADRLDPPLRRLIAVVLLGGIMGILDGSVVAVGVDTLAARFHASLSTIGWVSTGYLLALTIAIPITTWAVDRFG
ARRLWLTALVVFLSASLASGLAWNITSLIVFRVIQGLAAGVLDPLVLTLLARAAGPRRAGRVMGLMGMVLSAGPVLGLIV
GGIVLESASWRWMFLINLPVGVVALLGAARAVPADPPRQTPTSRLDVLGVALIGPGFAAVVLALTQAADRTAVVAWQVLV
PLVAAVTLLAGYAVHALRGRPHRSTPPLIDLRLFASPGFAASVAVMTLVGLTMFANLFVLPLYYQQQHGRGALGAGLLVA
PFAVAAVICMPLSGRLSDRLGPRALVRGGAVVAVLGQLAFTGIGAHTNEVWPALAAFVVGAGLSFVGAPTMGSLYRTLPP
ESVSQGSSVLYILNQLGAAIGIAVVTLILATVGPENTRGMAGFHGVHWFALAAVAVILAMSAFLPRRDATTEAAVPAAGA
ATATATATATTSTSTSTSTSGTVPPALSAATAQEGTR

Sequences:

>Translated_517_residues
MSAEARDADRLDPPLRRLIAVVLLGGIMGILDGSVVAVGVDTLAARFHASLSTIGWVSTGYLLALTIAIPITTWAVDRFG
ARRLWLTALVVFLSASLASGLAWNITSLIVFRVIQGLAAGVLDPLVLTLLARAAGPRRAGRVMGLMGMVLSAGPVLGLIV
GGIVLESASWRWMFLINLPVGVVALLGAARAVPADPPRQTPTSRLDVLGVALIGPGFAAVVLALTQAADRTAVVAWQVLV
PLVAAVTLLAGYAVHALRGRPHRSTPPLIDLRLFASPGFAASVAVMTLVGLTMFANLFVLPLYYQQQHGRGALGAGLLVA
PFAVAAVICMPLSGRLSDRLGPRALVRGGAVVAVLGQLAFTGIGAHTNEVWPALAAFVVGAGLSFVGAPTMGSLYRTLPP
ESVSQGSSVLYILNQLGAAIGIAVVTLILATVGPENTRGMAGFHGVHWFALAAVAVILAMSAFLPRRDATTEAAVPAAGA
ATATATATATTSTSTSTSTSGTVPPALSAATAQEGTR
>Mature_516_residues
SAEARDADRLDPPLRRLIAVVLLGGIMGILDGSVVAVGVDTLAARFHASLSTIGWVSTGYLLALTIAIPITTWAVDRFGA
RRLWLTALVVFLSASLASGLAWNITSLIVFRVIQGLAAGVLDPLVLTLLARAAGPRRAGRVMGLMGMVLSAGPVLGLIVG
GIVLESASWRWMFLINLPVGVVALLGAARAVPADPPRQTPTSRLDVLGVALIGPGFAAVVLALTQAADRTAVVAWQVLVP
LVAAVTLLAGYAVHALRGRPHRSTPPLIDLRLFASPGFAASVAVMTLVGLTMFANLFVLPLYYQQQHGRGALGAGLLVAP
FAVAAVICMPLSGRLSDRLGPRALVRGGAVVAVLGQLAFTGIGAHTNEVWPALAAFVVGAGLSFVGAPTMGSLYRTLPPE
SVSQGSSVLYILNQLGAAIGIAVVTLILATVGPENTRGMAGFHGVHWFALAAVAVILAMSAFLPRRDATTEAAVPAAGAA
TATATATATTSTSTSTSTSGTVPPALSAATAQEGTR

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=410, Percent_Identity=29.2682926829268, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1790195, Length=422, Percent_Identity=29.6208530805687, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI87081983, Length=440, Percent_Identity=29.0909090909091, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788710, Length=413, Percent_Identity=27.6029055690073, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788392, Length=457, Percent_Identity=26.6958424507659, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322958, Length=369, Percent_Identity=26.0162601626016, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319770, Length=413, Percent_Identity=25.6658595641646, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=306, Percent_Identity=25.8169934640523, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=372, Percent_Identity=23.9247311827957, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=294, Percent_Identity=22.7891156462585, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52810; Mature: 52679

Theoretical pI: Translated: 11.67; Mature: 11.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEARDADRLDPPLRRLIAVVLLGGIMGILDGSVVAVGVDTLAARFHASLSTIGWVSTG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
YLLALTIAIPITTWAVDRFGARRLWLTALVVFLSASLASGLAWNITSLIVFRVIQGLAAG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VLDPLVLTLLARAAGPRRAGRVMGLMGMVLSAGPVLGLIVGGIVLESASWRWMFLINLPV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECHH
GVVALLGAARAVPADPPRQTPTSRLDVLGVALIGPGFAAVVLALTQAADRTAVVAWQVLV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHH
PLVAAVTLLAGYAVHALRGRPHRSTPPLIDLRLFASPGFAASVAVMTLVGLTMFANLFVL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
PLYYQQQHGRGALGAGLLVAPFAVAAVICMPLSGRLSDRLGPRALVRGGAVVAVLGQLAF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHHHHHHH
TGIGAHTNEVWPALAAFVVGAGLSFVGAPTMGSLYRTLPPESVSQGSSVLYILNQLGAAI
HCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHCCCCHHHCCCCHHHHHHHHHHHHH
GIAVVTLILATVGPENTRGMAGFHGVHWFALAAVAVILAMSAFLPRRDATTEAAVPAAGA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCC
ATATATATATTSTSTSTSTSGTVPPALSAATAQEGTR
CHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
SAEARDADRLDPPLRRLIAVVLLGGIMGILDGSVVAVGVDTLAARFHASLSTIGWVSTG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
YLLALTIAIPITTWAVDRFGARRLWLTALVVFLSASLASGLAWNITSLIVFRVIQGLAAG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VLDPLVLTLLARAAGPRRAGRVMGLMGMVLSAGPVLGLIVGGIVLESASWRWMFLINLPV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECHH
GVVALLGAARAVPADPPRQTPTSRLDVLGVALIGPGFAAVVLALTQAADRTAVVAWQVLV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHH
PLVAAVTLLAGYAVHALRGRPHRSTPPLIDLRLFASPGFAASVAVMTLVGLTMFANLFVL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
PLYYQQQHGRGALGAGLLVAPFAVAAVICMPLSGRLSDRLGPRALVRGGAVVAVLGQLAF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHHHHHHH
TGIGAHTNEVWPALAAFVVGAGLSFVGAPTMGSLYRTLPPESVSQGSSVLYILNQLGAAI
HCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHCCCCHHHCCCCHHHHHHHHHHHHH
GIAVVTLILATVGPENTRGMAGFHGVHWFALAAVAVILAMSAFLPRRDATTEAAVPAAGA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCC
ATATATATATTSTSTSTSTSGTVPPALSAATAQEGTR
CHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]