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The map label for this gene is hcaE [H]

Identifier: 158315107

GI number: 158315107

Start: 3917945

End: 3919216

Strand: Direct

Name: hcaE [H]

Synonym: Franean1_3306

Alternate gene names: 158315107

Gene position: 3917945-3919216 (Clockwise)

Preceding gene: 158315106

Following gene: 158315108

Centisome position: 43.62

GC content: 65.17

Gene sequence:

>1272_bases
GTGGCACGATGGCCGAAGCCGGCTGAGGGAAGCTGGACGGAACACTACCCGGAGCTGGGAACCGGGCTGGTGTCGTACGA
GGACTCCATCTCGCCCGATTTCTACGCCCTGGAACGGGATGCGATCTTCAGGCGTGCCTGGCTGAACGTGGGCCGGGTCG
AGCAGTTACCGCGCAGCGGGAGCTACTTCACCAAGGAGATCGAGGCCGCCAGGGCCTCGATCGTCGTCGTGCGCGACAAC
GACGGTCAGGTCCGTGCCTTCCACAACATCTGTCGCCATCGCGGCAACAAGCTGGTGTGGAACGACTTCCCGCGGGAGGA
GACCGCCGGTAGCTGTCGCCAGTTCACCTGCAAGTACCACGGCTGGCGGTACGGCCTGGACGGCGCCGCCACCTTCGTGC
AGCAGGAGGGCGAGTTCTTCAACCTGGACAAGAAGGACTTCGGCCTCGTCCCGGTGCACTGCGACGTCTGGTCCGGATTC
ATTTTCGTCAATCTGGCGAAGGAGCCCGAACAGTCGCTGCCCGAGTTCCTCGGCCCGATGGTCACCGCGCTCGGCGGATA
TCCGTTCGACACGATGACCGAGCGCTTTTACTACCGTGCTGAGGTCGGCGCGAACTGGAAGCTGTTCATGGACGCCTTCC
AGGAGTTTTACCATGCGCCCATTCTGCACGCCCGGCAGACGCCGTCGAAGTTCTCGACGGCGGCGCAGCAGGCGGGGTTC
GAGGCGCCGCACTACCGCATCGACGGCCCGCACCGGCTGGTCAGCACGGCCGGTATCAAGGCGTGGGAACTGGACCCGGA
GATGCGCAAGCCGATGGAGGACATCACCCGCAGCGGCCTGTTCGGGCCCTGGGACGAACCCGATCTCGGCGTCGAGAAGA
TGCCGGCGGGTCTCAACCCGGCCGGCTGCGAGCCGTGGGGTCTGGACTCGTTCAATCTCTGGCCGAACTTCGTGATCCTG
ATCTGGGCGGGTGGCTGGTATCTCACCTACCATTACTGGCCGACGTCGCACAACACGCACACCTTCGAGGGGAACCTGTA
TTTCGTTCCCTCCCGGAACGCGCGGGACCGGGTTGCCCGCGAGATGGCCGCGGTGACCTTCAAGGAGTACGCGTTGCAGG
ACGCCAACACGCTGGAGGCGACGCAGTCGATGCTCGAGTCCCGGGCTGTCGCCGAGTTCCCACTGAACGACCAGGAGGTT
CTCTGCCGGCACCTGCACAAGGTCGCGGCCGACTGGGTCGGGGAATACCAGCACAACGGCGCGGGGGCGTGA

Upstream 100 bases:

>100_bases
CGCTACCTGGCAGCCGTGCCCGGCCTACCTGACCGGAGCCGGACCCGCGTCCGGCAGCTGACCCATCCGAAACGGACCCA
TCCGAAACGGAGGAATGACT

Downstream 100 bases:

>100_bases
GCCCGATGCCGACCCTGCTGCCGGGCGAGTTCGCCGATCTCGAGCCCTACGCCACGGTCTGGTGCCTGGCGACGGAGGCC
GAGCGTTACGGGAGGCGGCT

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MARWPKPAEGSWTEHYPELGTGLVSYEDSISPDFYALERDAIFRRAWLNVGRVEQLPRSGSYFTKEIEAARASIVVVRDN
DGQVRAFHNICRHRGNKLVWNDFPREETAGSCRQFTCKYHGWRYGLDGAATFVQQEGEFFNLDKKDFGLVPVHCDVWSGF
IFVNLAKEPEQSLPEFLGPMVTALGGYPFDTMTERFYYRAEVGANWKLFMDAFQEFYHAPILHARQTPSKFSTAAQQAGF
EAPHYRIDGPHRLVSTAGIKAWELDPEMRKPMEDITRSGLFGPWDEPDLGVEKMPAGLNPAGCEPWGLDSFNLWPNFVIL
IWAGGWYLTYHYWPTSHNTHTFEGNLYFVPSRNARDRVAREMAAVTFKEYALQDANTLEATQSMLESRAVAEFPLNDQEV
LCRHLHKVAADWVGEYQHNGAGA

Sequences:

>Translated_423_residues
MARWPKPAEGSWTEHYPELGTGLVSYEDSISPDFYALERDAIFRRAWLNVGRVEQLPRSGSYFTKEIEAARASIVVVRDN
DGQVRAFHNICRHRGNKLVWNDFPREETAGSCRQFTCKYHGWRYGLDGAATFVQQEGEFFNLDKKDFGLVPVHCDVWSGF
IFVNLAKEPEQSLPEFLGPMVTALGGYPFDTMTERFYYRAEVGANWKLFMDAFQEFYHAPILHARQTPSKFSTAAQQAGF
EAPHYRIDGPHRLVSTAGIKAWELDPEMRKPMEDITRSGLFGPWDEPDLGVEKMPAGLNPAGCEPWGLDSFNLWPNFVIL
IWAGGWYLTYHYWPTSHNTHTFEGNLYFVPSRNARDRVAREMAAVTFKEYALQDANTLEATQSMLESRAVAEFPLNDQEV
LCRHLHKVAADWVGEYQHNGAGA
>Mature_422_residues
ARWPKPAEGSWTEHYPELGTGLVSYEDSISPDFYALERDAIFRRAWLNVGRVEQLPRSGSYFTKEIEAARASIVVVRDND
GQVRAFHNICRHRGNKLVWNDFPREETAGSCRQFTCKYHGWRYGLDGAATFVQQEGEFFNLDKKDFGLVPVHCDVWSGFI
FVNLAKEPEQSLPEFLGPMVTALGGYPFDTMTERFYYRAEVGANWKLFMDAFQEFYHAPILHARQTPSKFSTAAQQAGFE
APHYRIDGPHRLVSTAGIKAWELDPEMRKPMEDITRSGLFGPWDEPDLGVEKMPAGLNPAGCEPWGLDSFNLWPNFVILI
WAGGWYLTYHYWPTSHNTHTFEGNLYFVPSRNARDRVAREMAAVTFKEYALQDANTLEATQSMLESRAVAEFPLNDQEVL
CRHLHKVAADWVGEYQHNGAGA

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788103, Length=189, Percent_Identity=31.2169312169312, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788888, Length=217, Percent_Identity=32.258064516129, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 48297; Mature: 48165

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARWPKPAEGSWTEHYPELGTGLVSYEDSISPDFYALERDAIFRRAWLNVGRVEQLPRSG
CCCCCCCCCCCCHHHCHHHCCCEEECCCCCCCCCEEHHHHHHHHHHHHCCHHHHHCCCCC
SYFTKEIEAARASIVVVRDNDGQVRAFHNICRHRGNKLVWNDFPREETAGSCRQFTCKYH
CHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCHHHCCCHHHEEEEEC
GWRYGLDGAATFVQQEGEFFNLDKKDFGLVPVHCDVWSGFIFVNLAKEPEQSLPEFLGPM
CEEECCCHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
VTALGGYPFDTMTERFYYRAEVGANWKLFMDAFQEFYHAPILHARQTPSKFSTAAQQAGF
HHHHCCCCHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCCC
EAPHYRIDGPHRLVSTAGIKAWELDPEMRKPMEDITRSGLFGPWDEPDLGVEKMPAGLNP
CCCCEEECCHHHHHHHCCCEEEECCHHHHCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
AGCEPWGLDSFNLWPNFVILIWAGGWYLTYHYWPTSHNTHTFEGNLYFVPSRNARDRVAR
CCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEECCCCCCEEECCEEEEEECCCHHHHHHH
EMAAVTFKEYALQDANTLEATQSMLESRAVAEFPLNDQEVLCRHLHKVAADWVGEYQHNG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
AGA
CCC
>Mature Secondary Structure 
ARWPKPAEGSWTEHYPELGTGLVSYEDSISPDFYALERDAIFRRAWLNVGRVEQLPRSG
CCCCCCCCCCCHHHCHHHCCCEEECCCCCCCCCEEHHHHHHHHHHHHCCHHHHHCCCCC
SYFTKEIEAARASIVVVRDNDGQVRAFHNICRHRGNKLVWNDFPREETAGSCRQFTCKYH
CHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCHHHCCCHHHEEEEEC
GWRYGLDGAATFVQQEGEFFNLDKKDFGLVPVHCDVWSGFIFVNLAKEPEQSLPEFLGPM
CEEECCCHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
VTALGGYPFDTMTERFYYRAEVGANWKLFMDAFQEFYHAPILHARQTPSKFSTAAQQAGF
HHHHCCCCHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCCC
EAPHYRIDGPHRLVSTAGIKAWELDPEMRKPMEDITRSGLFGPWDEPDLGVEKMPAGLNP
CCCCEEECCHHHHHHHCCCEEEECCHHHHCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
AGCEPWGLDSFNLWPNFVILIWAGGWYLTYHYWPTSHNTHTFEGNLYFVPSRNARDRVAR
CCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEECCCCCCEEECCEEEEEECCCHHHHHHH
EMAAVTFKEYALQDANTLEATQSMLESRAVAEFPLNDQEVLCRHLHKVAADWVGEYQHNG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
AGA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]